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The map label for this gene is emrB [H]

Identifier: 238027052

GI number: 238027052

Start: 1574911

End: 1576470

Strand: Reverse

Name: emrB [H]

Synonym: bglu_1g14310

Alternate gene names: 238027052

Gene position: 1576470-1574911 (Counterclockwise)

Preceding gene: 238027053

Following gene: 238027051

Centisome position: 40.35

GC content: 68.78

Gene sequence:

>1560_bases
ATGAGCAGCCTCCTCTCCGCCCGGTCGCCGCACGGCAGTCTCGCCGCACCGTGCGGCGGGCTGTTTCGCTGGGCGCTGGC
GGTCGTCACCGGCCTCGACTATTTCGACAACGCGCTGTTCTCGTTCTTCGCCAGCTACATCGCCGGCGGGATCAACGCCT
CGCCCGACGAACTCGTCTGGGCGTCGAGCGCCTATGCGATCACGGCGGTGCTCGGCATCCTGCAGCAGCAATGGTGGATC
GACCGCGTCGGCCATCGGCGCTACGTGAGCGGCTGCCTGCTGCTCTACGCGCTCGGCGCGAGCGCCGCGGCGCTGGCCGA
CACCTCGCTCGAACTCGCGTTCGCTCGGGGCTTCCAGGGCTACTTCATCGGCCCGATGATGGGTGCCTGCCGCATCCTGA
TCCAAATCACCTACCCGCCGCAGGAGCGCGCGCCGGCATTGCGCGCGTTCCTCGTCATGCTGCTGCTCGGCACCTCGCTC
GCGCCGATCGTCGGCGGCGAACTGATCGCGCGCTTCACTTGGCGCGCCGTGTTCGCCTGCACGGTGCCGATGGCGCTCGC
GAGCTCCGCGCTGGCCTACGCCACGCTGCCCGATGCCGGCCATGCGCCGCCCGCCGAGCGCACCCCGAGCCAGATCTGGC
CCTACGTGATCTTCGCCTTCGCGCAGGGCTCGCTGCAGATCGTGATGCAGCAGGTGCGCTACCAGCTGTTCCTGTCGTCG
CCTTTGCTGATCCTGCTGACGTTGGCCGGCTTCGCGGCGCTGGCGCTGTTCGCGCGCCACCAATGGAGCCAGCCTGCGCC
GCTGGTGCGCATGTCGGCGCTGCGGCTGCGCACGTTCCAGGTCGGCCTGCTGCTCTACGTGCTCTATTACTACATCACCA
CCTCGTTCAGCTATCTGACCTCGCGCCTTCTCGAGGCGGGGCTCGACTACCCCGTCGAGAACGCCGGGCGGCTGACGGGC
GCCACCTCGCTGATCGCGTCCGCCGCGCTGTTCGTCTACCTGCGCTACGCGAAGCACGTCACGCACAAGAAATGGTTCAT
CGTGCCGGGCTTCGCGCTCGCGGCCGGCGCCGCGCTCGCGATGGCGCGGCTGTCGCCCGGCGTCGGCGAGGCCGAGCTGA
TCGTGCCGCTGCTGATGCGCGGCCTGTTGCTGCTGTTCATCGTGATGCCGGTCGCGAACCTGACGTTTCGTGCATTCCCG
ATCGACGACTACACGCACAGCTACCGCCTGAAGAACATCGTGCGGCAACTGACGCTGTCGTTCGCGACCTCCTCGGTGAT
CATCATCGAGCAGCATCGCGACGCGCTGCACCGCGCACGGCTTTTCGAGCAGGCGAGCGTGTTCAACCCCACGTTCGCGC
AGACCGTCGACGCGCTGGCGCGCGGCTACGCCGGCGCCGGTCAGGCGGCGGGCGACGCGCACGCGCTCGCGCTGGCGTCG
GTCAGCGCAACGATCGGGCGCCAGGCCGCGTTCCTGACTTCGCTGGACGGCTTCCTGTTCATCGCCGGCGTCGCGCTCTG
CGGCGGCCTGTTCGCCGCCTGGCAAAAATCCATCGATTGA

Upstream 100 bases:

>100_bases
TCGACTACGTCGAGCGACGCCTGGCCGGCGAAGATCCCGATACGCTGATCGCGCGCCGCCAGGCGGCCGCGAAGCAGGGC
CGCGGCCGCAGGTCACGGCC

Downstream 100 bases:

>100_bases
GGTACCGTGCGCTGTCTCGTCACACCCCTCCCGCATCGATGCTCTCCCGACTCACCCGGTGGCTCGACGCCCGCCGCCGC
GACCGCGCGCTGCGCGCGTT

Product: major facilitator family transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MSSLLSARSPHGSLAAPCGGLFRWALAVVTGLDYFDNALFSFFASYIAGGINASPDELVWASSAYAITAVLGILQQQWWI
DRVGHRRYVSGCLLLYALGASAAALADTSLELAFARGFQGYFIGPMMGACRILIQITYPPQERAPALRAFLVMLLLGTSL
APIVGGELIARFTWRAVFACTVPMALASSALAYATLPDAGHAPPAERTPSQIWPYVIFAFAQGSLQIVMQQVRYQLFLSS
PLLILLTLAGFAALALFARHQWSQPAPLVRMSALRLRTFQVGLLLYVLYYYITTSFSYLTSRLLEAGLDYPVENAGRLTG
ATSLIASAALFVYLRYAKHVTHKKWFIVPGFALAAGAALAMARLSPGVGEAELIVPLLMRGLLLLFIVMPVANLTFRAFP
IDDYTHSYRLKNIVRQLTLSFATSSVIIIEQHRDALHRARLFEQASVFNPTFAQTVDALARGYAGAGQAAGDAHALALAS
VSATIGRQAAFLTSLDGFLFIAGVALCGGLFAAWQKSID

Sequences:

>Translated_519_residues
MSSLLSARSPHGSLAAPCGGLFRWALAVVTGLDYFDNALFSFFASYIAGGINASPDELVWASSAYAITAVLGILQQQWWI
DRVGHRRYVSGCLLLYALGASAAALADTSLELAFARGFQGYFIGPMMGACRILIQITYPPQERAPALRAFLVMLLLGTSL
APIVGGELIARFTWRAVFACTVPMALASSALAYATLPDAGHAPPAERTPSQIWPYVIFAFAQGSLQIVMQQVRYQLFLSS
PLLILLTLAGFAALALFARHQWSQPAPLVRMSALRLRTFQVGLLLYVLYYYITTSFSYLTSRLLEAGLDYPVENAGRLTG
ATSLIASAALFVYLRYAKHVTHKKWFIVPGFALAAGAALAMARLSPGVGEAELIVPLLMRGLLLLFIVMPVANLTFRAFP
IDDYTHSYRLKNIVRQLTLSFATSSVIIIEQHRDALHRARLFEQASVFNPTFAQTVDALARGYAGAGQAAGDAHALALAS
VSATIGRQAAFLTSLDGFLFIAGVALCGGLFAAWQKSID
>Mature_518_residues
SSLLSARSPHGSLAAPCGGLFRWALAVVTGLDYFDNALFSFFASYIAGGINASPDELVWASSAYAITAVLGILQQQWWID
RVGHRRYVSGCLLLYALGASAAALADTSLELAFARGFQGYFIGPMMGACRILIQITYPPQERAPALRAFLVMLLLGTSLA
PIVGGELIARFTWRAVFACTVPMALASSALAYATLPDAGHAPPAERTPSQIWPYVIFAFAQGSLQIVMQQVRYQLFLSSP
LLILLTLAGFAALALFARHQWSQPAPLVRMSALRLRTFQVGLLLYVLYYYITTSFSYLTSRLLEAGLDYPVENAGRLTGA
TSLIASAALFVYLRYAKHVTHKKWFIVPGFALAAGAALAMARLSPGVGEAELIVPLLMRGLLLLFIVMPVANLTFRAFPI
DDYTHSYRLKNIVRQLTLSFATSSVIIIEQHRDALHRARLFEQASVFNPTFAQTVDALARGYAGAGQAAGDAHALALASV
SATIGRQAAFLTSLDGFLFIAGVALCGGLFAAWQKSID

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=480, Percent_Identity=22.2916666666667, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI1788710, Length=488, Percent_Identity=21.7213114754098, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1790195, Length=434, Percent_Identity=23.0414746543779, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI87081983, Length=280, Percent_Identity=25, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56037; Mature: 55906

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLLSARSPHGSLAAPCGGLFRWALAVVTGLDYFDNALFSFFASYIAGGINASPDELVW
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
ASSAYAITAVLGILQQQWWIDRVGHRRYVSGCLLLYALGASAAALADTSLELAFARGFQG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
YFIGPMMGACRILIQITYPPQERAPALRAFLVMLLLGTSLAPIVGGELIARFTWRAVFAC
EEHHHHHHHEEEEEEEECCCHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
TVPMALASSALAYATLPDAGHAPPAERTPSQIWPYVIFAFAQGSLQIVMQQVRYQLFLSS
HHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
PLLILLTLAGFAALALFARHQWSQPAPLVRMSALRLRTFQVGLLLYVLYYYITTSFSYLT
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRLLEAGLDYPVENAGRLTGATSLIASAALFVYLRYAKHVTHKKWFIVPGFALAAGAALA
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHH
MARLSPGVGEAELIVPLLMRGLLLLFIVMPVANLTFRAFPIDDYTHSYRLKNIVRQLTLS
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHH
FATSSVIIIEQHRDALHRARLFEQASVFNPTFAQTVDALARGYAGAGQAAGDAHALALAS
HHCCEEEEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
VSATIGRQAAFLTSLDGFLFIAGVALCGGLFAAWQKSID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSLLSARSPHGSLAAPCGGLFRWALAVVTGLDYFDNALFSFFASYIAGGINASPDELVW
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
ASSAYAITAVLGILQQQWWIDRVGHRRYVSGCLLLYALGASAAALADTSLELAFARGFQG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
YFIGPMMGACRILIQITYPPQERAPALRAFLVMLLLGTSLAPIVGGELIARFTWRAVFAC
EEHHHHHHHEEEEEEEECCCHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
TVPMALASSALAYATLPDAGHAPPAERTPSQIWPYVIFAFAQGSLQIVMQQVRYQLFLSS
HHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
PLLILLTLAGFAALALFARHQWSQPAPLVRMSALRLRTFQVGLLLYVLYYYITTSFSYLT
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRLLEAGLDYPVENAGRLTGATSLIASAALFVYLRYAKHVTHKKWFIVPGFALAAGAALA
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHH
MARLSPGVGEAELIVPLLMRGLLLLFIVMPVANLTFRAFPIDDYTHSYRLKNIVRQLTLS
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHH
FATSSVIIIEQHRDALHRARLFEQASVFNPTFAQTVDALARGYAGAGQAAGDAHALALAS
HHCCEEEEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
VSATIGRQAAFLTSLDGFLFIAGVALCGGLFAAWQKSID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]