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The map label for this gene is holB [H]

Identifier: 238027002

GI number: 238027002

Start: 1521813

End: 1522841

Strand: Direct

Name: holB [H]

Synonym: bglu_1g13800

Alternate gene names: 238027002

Gene position: 1521813-1522841 (Clockwise)

Preceding gene: 238027001

Following gene: 238027003

Centisome position: 38.96

GC content: 73.66

Gene sequence:

>1029_bases
ATGATCTATCCGTGGCAAACCGACGACTGGGACCGGCTGCAGGCGCTGCGCGCGCACTGGCCGCATGCGCTGCTGCTGCA
CGGCCAGGCCGGCATCGGCAAGCTGCGCTTCGCCCAGCACCTGGCGCAGGGCCTGCTCTGCGAGTCGCCGCAGGGTAACG
GCGAGCCCTGCGGCACCTGCCCGGCCTGCACCTGGTTCGGGCAGGGCAACCATCCCGATTACCGGATCGTGCTGCCCGAG
GCGCTGGCGGGCGAAGCGCCGGGCGCCGCGGCCGAGGAGCCGAAGGCCGATGCCGACGAGGGCGGCAAGAAAACCCGCAC
GCCCAGCAAGGAGATCAAGATCGAGCAGGTGCGCGGCTTGCTGGACTTCGTCGGGGTCGGCTCGCATCGCGGCGGCGCGC
GCGTGGTGGTGCTCTATCCTGCCGAGGCGCTCAACGTCGCGGCCGCCAACGCGCTGTTGAAGACGCTCGAGGAGCCCCCC
GCGGGCGTCGTGTTCCTGCTCGTCTCGGCGCGGCTCGACCGGCTGCTGCCGACCATCGTCAGCCGCTGCCGGCAGTGGCC
GATGACGGTGCCCTCGCACGCGGCGGCGGTGGCCTGGCTCGAGGCCCAGCAGGTGGGCGACGCGGCCGTGCTGCTGGCCG
AGGCCGGCGGCGCGCCGCTGGCCGCGCTCGCGCTGGCGAGCGACGAGCACCGGCCGCTGCGCGACTGGACGCTCGCGCAG
CTCGCGGCCGGCGCGCGCTGCGATCCGTTCGCCTGCGGCGAGTCGCTGCAGAAGCTGCCGGTGCCGCTCGTGCTCGGCTG
GCTGCAGCGCTGGCTCTACGACCTGCTCGCCGAGCGCTGCGCCGGCCGGCCGCGCTATTTCCCGGCGCAGCGCGACGCGC
TCGCGCGCTGCGCGGCCGCGCTCGACGCCGAGGCCTTCACACGCTTCATGAAGACCGTCACGCGGCAGCGCATGGTGGAG
AACCACCCGCTCAACGCGCGGCTCGTGTTCGAGGAACTGTTCCTCGGCTACCGCGCGCTCTACGCCTGA

Upstream 100 bases:

>100_bases
CGCAGATCCAGCGGCAGCTCGAAGGCGTGCTGGCCGCGCTCTGATGCCGGCCGCCGGCAACCCTTAAGACCCGCTCATCC
GACCAGAGGCCGACAAACCG

Downstream 100 bases:

>100_bases
GCCCGGCCGCCGGCCGGCCCCGAACCCAGACAGGAAGCGCAGATGTTCGTCGATTCCCATTGCCACATCAATTTCGAAGG
CCTCGCCGAGCGCCTGCCCG

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MIYPWQTDDWDRLQALRAHWPHALLLHGQAGIGKLRFAQHLAQGLLCESPQGNGEPCGTCPACTWFGQGNHPDYRIVLPE
ALAGEAPGAAAEEPKADADEGGKKTRTPSKEIKIEQVRGLLDFVGVGSHRGGARVVVLYPAEALNVAAANALLKTLEEPP
AGVVFLLVSARLDRLLPTIVSRCRQWPMTVPSHAAAVAWLEAQQVGDAAVLLAEAGGAPLAALALASDEHRPLRDWTLAQ
LAAGARCDPFACGESLQKLPVPLVLGWLQRWLYDLLAERCAGRPRYFPAQRDALARCAAALDAEAFTRFMKTVTRQRMVE
NHPLNARLVFEELFLGYRALYA

Sequences:

>Translated_342_residues
MIYPWQTDDWDRLQALRAHWPHALLLHGQAGIGKLRFAQHLAQGLLCESPQGNGEPCGTCPACTWFGQGNHPDYRIVLPE
ALAGEAPGAAAEEPKADADEGGKKTRTPSKEIKIEQVRGLLDFVGVGSHRGGARVVVLYPAEALNVAAANALLKTLEEPP
AGVVFLLVSARLDRLLPTIVSRCRQWPMTVPSHAAAVAWLEAQQVGDAAVLLAEAGGAPLAALALASDEHRPLRDWTLAQ
LAAGARCDPFACGESLQKLPVPLVLGWLQRWLYDLLAERCAGRPRYFPAQRDALARCAAALDAEAFTRFMKTVTRQRMVE
NHPLNARLVFEELFLGYRALYA
>Mature_342_residues
MIYPWQTDDWDRLQALRAHWPHALLLHGQAGIGKLRFAQHLAQGLLCESPQGNGEPCGTCPACTWFGQGNHPDYRIVLPE
ALAGEAPGAAAEEPKADADEGGKKTRTPSKEIKIEQVRGLLDFVGVGSHRGGARVVVLYPAEALNVAAANALLKTLEEPP
AGVVFLLVSARLDRLLPTIVSRCRQWPMTVPSHAAAVAWLEAQQVGDAAVLLAEAGGAPLAALALASDEHRPLRDWTLAQ
LAAGARCDPFACGESLQKLPVPLVLGWLQRWLYDLLAERCAGRPRYFPAQRDALARCAAALDAEAFTRFMKTVTRQRMVE
NHPLNARLVFEELFLGYRALYA

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=283, Percent_Identity=34.982332155477, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37223; Mature: 37223

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYPWQTDDWDRLQALRAHWPHALLLHGQAGIGKLRFAQHLAQGLLCESPQGNGEPCGTC
CCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCC
PACTWFGQGNHPDYRIVLPEALAGEAPGAAAEEPKADADEGGKKTRTPSKEIKIEQVRGL
CCEEECCCCCCCCEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LDFVGVGSHRGGARVVVLYPAEALNVAAANALLKTLEEPPAGVVFLLVSARLDRLLPTIV
HHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
SRCRQWPMTVPSHAAAVAWLEAQQVGDAAVLLAEAGGAPLAALALASDEHRPLRDWTLAQ
HHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHCCCCCCCCHHHHHHH
LAAGARCDPFACGESLQKLPVPLVLGWLQRWLYDLLAERCAGRPRYFPAQRDALARCAAA
HHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
LDAEAFTRFMKTVTRQRMVENHPLNARLVFEELFLGYRALYA
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIYPWQTDDWDRLQALRAHWPHALLLHGQAGIGKLRFAQHLAQGLLCESPQGNGEPCGTC
CCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCC
PACTWFGQGNHPDYRIVLPEALAGEAPGAAAEEPKADADEGGKKTRTPSKEIKIEQVRGL
CCEEECCCCCCCCEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LDFVGVGSHRGGARVVVLYPAEALNVAAANALLKTLEEPPAGVVFLLVSARLDRLLPTIV
HHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
SRCRQWPMTVPSHAAAVAWLEAQQVGDAAVLLAEAGGAPLAALALASDEHRPLRDWTLAQ
HHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHCCCCCCCCHHHHHHH
LAAGARCDPFACGESLQKLPVPLVLGWLQRWLYDLLAERCAGRPRYFPAQRDALARCAAA
HHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
LDAEAFTRFMKTVTRQRMVENHPLNARLVFEELFLGYRALYA
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]