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The map label for this gene is estA [H]

Identifier: 238026977

GI number: 238026977

Start: 1487203

End: 1487874

Strand: Reverse

Name: estA [H]

Synonym: bglu_1g13540

Alternate gene names: 238026977

Gene position: 1487874-1487203 (Counterclockwise)

Preceding gene: 238026978

Following gene: 238026971

Centisome position: 38.09

GC content: 68.01

Gene sequence:

>672_bases
ATGCGCCCCCTCGACACCCTCGAAGTCGAAACCTCCGCCGATCCCCGCTACGCGGTCATCCTCATGCATGGCCTCGGCGC
CGATGCCAACGACTTCGTGCCGCTGATCCCAGAGCTGCATCTGGCCGACGCGCCCGGCGTGCGCTTCGTGTTGCCGAACG
CGCCCGAGATGGCCGTCACCGCCAATAACGGCTACGTGATGCGCGCCTGGTACGACATCCTGTCGTTCAACGGCGGCCTC
AGCCGCGAAGTCGACGAAGCGGGCATCGAGGCCTCGCGCGACGCCATCCGTGCGCTGATCGAGGCGCAGAACCAGCGCGG
CATCCCGACGTCGCGGATTTTCCTGGCCGGCTTCTCGCAAGGTGGCGCGATGACGTGGACCGCGGGCCTCACGCACCCTG
ACGCGCTCGCCGGACTGATCGTGCTGTCAGGCTATCTGCCCTCGCCGGCACTGGTCGCACGTGATTTCCAGGCGGCCAAC
CGCGACACGCCGATCTTCGCCGCGCACGGCAGCTTCGACGACGTGCTGCCGCCGCGGCTCGGCGAGGCGGCCCGCGATTT
CGCGCTCGATCGGGGATGCAAGGTGGATTGGCAGGTCTATCCGATGCCGCACTCGACCTGCCTGGAAGAAGTCGTCGCGC
TGCGCGCGTGGCTGCTCAAGCGACTCGTCTGA

Upstream 100 bases:

>100_bases
CAACGGCGCGCTTCACGATAGTGCCGCGCCGGTGTTAGCGTTAGGCTCTCGCGCGGTGCCGGCCGGCGGCACCGCCCTCT
CTTTACGCCACGGAACGACC

Downstream 100 bases:

>100_bases
AGCGGCAGCGACGAGGTGCGTCGCCGAGGCACCGGCCAGCACGCCACTCGGGCAGCGAACATGAAAAAACCCGCAGGGCC
ACCGGCCATGCGGGTTTCGA

Product: phospholipase/carboxylesterase

Products: NA

Alternate protein names: Esterase I [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MRPLDTLEVETSADPRYAVILMHGLGADANDFVPLIPELHLADAPGVRFVLPNAPEMAVTANNGYVMRAWYDILSFNGGL
SREVDEAGIEASRDAIRALIEAQNQRGIPTSRIFLAGFSQGGAMTWTAGLTHPDALAGLIVLSGYLPSPALVARDFQAAN
RDTPIFAAHGSFDDVLPPRLGEAARDFALDRGCKVDWQVYPMPHSTCLEEVVALRAWLLKRLV

Sequences:

>Translated_223_residues
MRPLDTLEVETSADPRYAVILMHGLGADANDFVPLIPELHLADAPGVRFVLPNAPEMAVTANNGYVMRAWYDILSFNGGL
SREVDEAGIEASRDAIRALIEAQNQRGIPTSRIFLAGFSQGGAMTWTAGLTHPDALAGLIVLSGYLPSPALVARDFQAAN
RDTPIFAAHGSFDDVLPPRLGEAARDFALDRGCKVDWQVYPMPHSTCLEEVVALRAWLLKRLV
>Mature_223_residues
MRPLDTLEVETSADPRYAVILMHGLGADANDFVPLIPELHLADAPGVRFVLPNAPEMAVTANNGYVMRAWYDILSFNGGL
SREVDEAGIEASRDAIRALIEAQNQRGIPTSRIFLAGFSQGGAMTWTAGLTHPDALAGLIVLSGYLPSPALVARDFQAAN
RDTPIFAAHGSFDDVLPPRLGEAARDFALDRGCKVDWQVYPMPHSTCLEEVVALRAWLLKRLV

Specific function: Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI9966764, Length=214, Percent_Identity=38.3177570093458, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI5453722, Length=211, Percent_Identity=33.6492890995261, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI20270341, Length=209, Percent_Identity=22.9665071770335, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17508057, Length=211, Percent_Identity=32.2274881516588, Blast_Score=113, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6323147, Length=227, Percent_Identity=34.3612334801762, Blast_Score=127, Evalue=2e-30,
Organism=Drosophila melanogaster, GI45553055, Length=207, Percent_Identity=31.8840579710145, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI45553057, Length=207, Percent_Identity=31.8840579710145, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21357257, Length=207, Percent_Identity=31.8840579710145, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 24207; Mature: 24207

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPLDTLEVETSADPRYAVILMHGLGADANDFVPLIPELHLADAPGVRFVLPNAPEMAVT
CCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCEEECCCCCEEEECCCCCCEEEE
ANNGYVMRAWYDILSFNGGLSREVDEAGIEASRDAIRALIEAQNQRGIPTSRIFLAGFSQ
CCCCEEEEEEHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCHHEEEEEECCC
GGAMTWTAGLTHPDALAGLIVLSGYLPSPALVARDFQAANRDTPIFAAHGSFDDVLPPRL
CCEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCH
GEAARDFALDRGCKVDWQVYPMPHSTCLEEVVALRAWLLKRLV
HHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRPLDTLEVETSADPRYAVILMHGLGADANDFVPLIPELHLADAPGVRFVLPNAPEMAVT
CCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCEEECCCCCEEEECCCCCCEEEE
ANNGYVMRAWYDILSFNGGLSREVDEAGIEASRDAIRALIEAQNQRGIPTSRIFLAGFSQ
CCCCEEEEEEHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCHHEEEEEECCC
GGAMTWTAGLTHPDALAGLIVLSGYLPSPALVARDFQAANRDTPIFAAHGSFDDVLPPRL
CCEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCH
GEAARDFALDRGCKVDWQVYPMPHSTCLEEVVALRAWLLKRLV
HHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA