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The map label for this gene is pucI [H]

Identifier: 238026938

GI number: 238026938

Start: 1433386

End: 1434870

Strand: Direct

Name: pucI [H]

Synonym: bglu_1g13100

Alternate gene names: 238026938

Gene position: 1433386-1434870 (Clockwise)

Preceding gene: 238026937

Following gene: 238026939

Centisome position: 36.69

GC content: 67.27

Gene sequence:

>1485_bases
ATGGCTCAGTTCAGCATTGCGCCGGAAAGCCCCCCCTATCATCCGGACTCCACGAACCGTGCGGACCCTGGCCCGGGTTC
GCCGGGCCGCTACAGCGAGCGGCTCTACAACGACGATCTCGCGCCGCTCGAGCGGCAGGGCTGGGGCGCCTATAACATCT
TCGCGTTCTGGATGTCGGACGTCCACAGCGTGGGCGGCTACGTGTTCGCGGGCAGCCTGTTCGCACTCGGCCTGACGAGC
TGGCAGGTGCTGGTGTCGCTGCTGGTCGGGATCACCATCGTCAACCTGCTCTGCAACCTGATCGCCAAGCCGAGCCAGCA
GTTGGGCGTGCCGTATCCGGTGGCCTGCCGCGCCACCTTCGGCGTACTCGGCGCGAACGTGCCGGCCGTGATCCGCGGGC
TGATCGCCGTGGCCTGGTACGGCATCCAGACCTATCTGGCCTCGAGCGCGCTGGTGGTGGTGGTGCTGAAGTTCGTGCCG
CAGTGGATGCCCTACGCGGATGTTCATCAGCACGGCCTGCTCGGGCTGTCCGCGCTCGGCTGGGCCGGCTTCATGCTGCT
CTGGGTGCTGCAGGCGCTGGTGTTCTGGAACGGCATGGAGACCATCAAGAAGTTCATCGACTTCGCGGGGCCGGCCGTCT
ACGTGGTGATGTTCGCGCTGGCCGGCTACATGGTCTGGCGCGCCGGCTGGCGCAACATCGGCATCAACCTGGGCGGCGTG
CGCTACCACGGTGCCGAGGCGATCCCGGTGATGCTCACGGCGATCGCGCTGGTGGTCTCGTACTTCTCCGGGCCGATGCT
CAATTTCGGCGATTTCTCGCGCTACTGCGGCAGCTTCGGCGCGGTGAAGCGCGGCAATTTCTGGGGCCTGCCGGTCAATT
TCCTGGCGTTCTCGCTGGTCACCGTGATCACCACGGCCGCCACGCTGCCGGTGTTCGGCGAACTGCTGACCGACCCGGTC
GCCACCGTCGGACGCATCGATCATCCGACCGCCGTGATCCTCGGCGCGCTGACCTTCACGGTCGCCACCATCGGCATCAA
CATCGTCGCGAACTTCGTGTCGCCCGCCTTCGATTTCTCCAACGTCGCGCCGCGGCGCATCAGCTGGCGCGCGGGCGGCA
TGCTGGCCGCGGTCGCCTCGGTGTTCATCACGCCCTGGAACCTGTTCAACAATCCGGCGGTGATCCACTATACGCTCGAC
GTGCTCGGCAGCTTCATCGGGCCGCTGTACGGCGTGCTGATCGTCGATTTCTACCTGATCAAGCGCGGCCGGCTGCTGCG
CGACGAGCTCTACACGCTGTCGGAGACGGGCGCCTACTGGTATCGCGGCGGCGTGAACCGGCGCGCGGTGCTGGCGCTGC
TGCCGGCCGCCGCGCTGGCCGTGGCCTGCGTGATGGTGCCGGCGCTGGCCGGCCTCGCGAACTTCTCGTGGTTCGTCGGC
GCGCTGCTGGGCGGCCTGTTCTATCGGATCATGGCGCGGCACTGA

Upstream 100 bases:

>100_bases
GGCAGCCTCGCGCTGCCGCAGGCGGGCGCGGCCCCACACGAGGCGGGCCGCGGTTTCAACGACGATCGCGCGGCCCGACC
GCGGCAGGCAAGGAGATGTC

Downstream 100 bases:

>100_bases
ACCAGGACGACCACATGAAGATCAAGCTGATCAACCCGAACACCACGCAGCGCATGACCGAGGCGATGGGGCGCTGCGCG
CGCGAGACGGTGAACGCCGG

Product: NCS1 nucleoside transporter family protein

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MAQFSIAPESPPYHPDSTNRADPGPGSPGRYSERLYNDDLAPLERQGWGAYNIFAFWMSDVHSVGGYVFAGSLFALGLTS
WQVLVSLLVGITIVNLLCNLIAKPSQQLGVPYPVACRATFGVLGANVPAVIRGLIAVAWYGIQTYLASSALVVVVLKFVP
QWMPYADVHQHGLLGLSALGWAGFMLLWVLQALVFWNGMETIKKFIDFAGPAVYVVMFALAGYMVWRAGWRNIGINLGGV
RYHGAEAIPVMLTAIALVVSYFSGPMLNFGDFSRYCGSFGAVKRGNFWGLPVNFLAFSLVTVITTAATLPVFGELLTDPV
ATVGRIDHPTAVILGALTFTVATIGINIVANFVSPAFDFSNVAPRRISWRAGGMLAAVASVFITPWNLFNNPAVIHYTLD
VLGSFIGPLYGVLIVDFYLIKRGRLLRDELYTLSETGAYWYRGGVNRRAVLALLPAAALAVACVMVPALAGLANFSWFVG
ALLGGLFYRIMARH

Sequences:

>Translated_494_residues
MAQFSIAPESPPYHPDSTNRADPGPGSPGRYSERLYNDDLAPLERQGWGAYNIFAFWMSDVHSVGGYVFAGSLFALGLTS
WQVLVSLLVGITIVNLLCNLIAKPSQQLGVPYPVACRATFGVLGANVPAVIRGLIAVAWYGIQTYLASSALVVVVLKFVP
QWMPYADVHQHGLLGLSALGWAGFMLLWVLQALVFWNGMETIKKFIDFAGPAVYVVMFALAGYMVWRAGWRNIGINLGGV
RYHGAEAIPVMLTAIALVVSYFSGPMLNFGDFSRYCGSFGAVKRGNFWGLPVNFLAFSLVTVITTAATLPVFGELLTDPV
ATVGRIDHPTAVILGALTFTVATIGINIVANFVSPAFDFSNVAPRRISWRAGGMLAAVASVFITPWNLFNNPAVIHYTLD
VLGSFIGPLYGVLIVDFYLIKRGRLLRDELYTLSETGAYWYRGGVNRRAVLALLPAAALAVACVMVPALAGLANFSWFVG
ALLGGLFYRIMARH
>Mature_493_residues
AQFSIAPESPPYHPDSTNRADPGPGSPGRYSERLYNDDLAPLERQGWGAYNIFAFWMSDVHSVGGYVFAGSLFALGLTSW
QVLVSLLVGITIVNLLCNLIAKPSQQLGVPYPVACRATFGVLGANVPAVIRGLIAVAWYGIQTYLASSALVVVVLKFVPQ
WMPYADVHQHGLLGLSALGWAGFMLLWVLQALVFWNGMETIKKFIDFAGPAVYVVMFALAGYMVWRAGWRNIGINLGGVR
YHGAEAIPVMLTAIALVVSYFSGPMLNFGDFSRYCGSFGAVKRGNFWGLPVNFLAFSLVTVITTAATLPVFGELLTDPVA
TVGRIDHPTAVILGALTFTVATIGINIVANFVSPAFDFSNVAPRRISWRAGGMLAAVASVFITPWNLFNNPAVIHYTLDV
LGSFIGPLYGVLIVDFYLIKRGRLLRDELYTLSETGAYWYRGGVNRRAVLALLPAAALAVACVMVPALAGLANFSWFVGA
LLGGLFYRIMARH

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=472, Percent_Identity=27.3305084745763, Blast_Score=180, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6319495, Length=452, Percent_Identity=25, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6322219, Length=453, Percent_Identity=23.841059602649, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6319429, Length=506, Percent_Identity=24.3083003952569, Blast_Score=129, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6323266, Length=472, Percent_Identity=22.0338983050847, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324766, Length=454, Percent_Identity=21.1453744493392, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6324645, Length=474, Percent_Identity=21.5189873417722, Blast_Score=88, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 53464; Mature: 53333

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHCC
>Mature Secondary Structure 
AQFSIAPESPPYHPDSTNRADPGPGSPGRYSERLYNDDLAPLERQGWGAYNIFAFWMSD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHCCCCHHHHHHHHHHH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HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]