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The map label for this gene is thyA [H]

Identifier: 238026573

GI number: 238026573

Start: 993404

End: 994375

Strand: Direct

Name: thyA [H]

Synonym: bglu_1g09170

Alternate gene names: 238026573

Gene position: 993404-994375 (Clockwise)

Preceding gene: 238026571

Following gene: 238026576

Centisome position: 25.43

GC content: 67.18

Gene sequence:

>972_bases
ATGAAACAGTATCTCGATCTCGTCCGCACCATCCTCGACACCGGCTCCTGGCAGGAAAACCGCACCGGCGTGCGCACCAT
CGGCATTCCCGGCGCGATGCTGCGCTTCGATCTCCAGCAGGGCTTTCCGGCGGTCACCACCAAGAAGCTCGCGTTCAAGT
CCGCCATCGGCGAACTGGTCGGCTTCCTGCGCGCCTCGCGCAGCGCCGCCGAGTTCCGCGCGCTCGGTTGCAAGGTCTGG
GATGCCAATGCGAACGAGAACGCCGCCTGGCTCGCTAACCCGTATCGCACCGGCGTGGACGACCTCGGCGACGTGTATGG
CGTGCAGTGGCGCCGCTGGCCCGGCTACAAGGTGCTCGACGCGCAGGCCACGGCGCAGCTCGAGGACGCGCTCGCGCGCG
GCTATCGCATCGTCACGCGCTTCGACGAGGCGGGCGCGCAAAAGCTGCTGTTGCACAAGGCGATCGACCAGCTGCGCGAC
TGCCTCGACACGATCATGCGCGACCCGTCGAGCCGGCGCATCCTGTTCCACGGCTGGAATCCCGTCAAGCTCGAGGAAAT
CGCGCTGCCGGCCTGTCATCTGCTCTATCAGTTCCTGCCGAATCTGGCGAAGCGCGAGATCTCGCTCTGCCTGTACATCC
GCAGCAACGACGTGGGGCTCGGCACGCCGTTCAACCTCGCGGAAGGTGCGGCGCTGCTGGAGCTCGTCGGCCGGCTGACG
GGATACCAGCCGCGCTGGTTCAGCTATTTCATCGGCGACGCGCACATCTACGAGAACCAGCTCGACATGCTGCGCCAGCA
GCTCGAGCGCGAGCCCTACGAGAGCCCGCGCCTCGAGATCGCCGAGCGCGTGCCCGACTACGCGAAGACGGGCGTCTACG
CGCCCGAATGGCTGGAGCGGATCGAGCCGGCCGATTTCACGCTCGCCGGTTACCGCCACCACGCGCCGCTGAGCGCGCCG
ATGGCGGTCTGA

Upstream 100 bases:

>100_bases
GGTGGCGCGTTGCCGGCTCGCTGCGCCCGCCGCCGGCCGCGCGGCGCGAGGCTTGTTAGAATCGCCGGGTGTGGCCGCGC
TCGCGGCCGAAGGAACCCGC

Downstream 100 bases:

>100_bases
GGCAGGCGCCGGGCAGGCGGCCCGGCAGCGCAGCGTCACCCGCCCGCGCGAGGTCGGCGCGGGCGGGTTGCTGCCGGCTC
GTTGCCGGAGCGGACCGGAC

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKQYLDLVRTILDTGSWQENRTGVRTIGIPGAMLRFDLQQGFPAVTTKKLAFKSAIGELVGFLRASRSAAEFRALGCKVW
DANANENAAWLANPYRTGVDDLGDVYGVQWRRWPGYKVLDAQATAQLEDALARGYRIVTRFDEAGAQKLLLHKAIDQLRD
CLDTIMRDPSSRRILFHGWNPVKLEEIALPACHLLYQFLPNLAKREISLCLYIRSNDVGLGTPFNLAEGAALLELVGRLT
GYQPRWFSYFIGDAHIYENQLDMLRQQLEREPYESPRLEIAERVPDYAKTGVYAPEWLERIEPADFTLAGYRHHAPLSAP
MAV

Sequences:

>Translated_323_residues
MKQYLDLVRTILDTGSWQENRTGVRTIGIPGAMLRFDLQQGFPAVTTKKLAFKSAIGELVGFLRASRSAAEFRALGCKVW
DANANENAAWLANPYRTGVDDLGDVYGVQWRRWPGYKVLDAQATAQLEDALARGYRIVTRFDEAGAQKLLLHKAIDQLRD
CLDTIMRDPSSRRILFHGWNPVKLEEIALPACHLLYQFLPNLAKREISLCLYIRSNDVGLGTPFNLAEGAALLELVGRLT
GYQPRWFSYFIGDAHIYENQLDMLRQQLEREPYESPRLEIAERVPDYAKTGVYAPEWLERIEPADFTLAGYRHHAPLSAP
MAV
>Mature_323_residues
MKQYLDLVRTILDTGSWQENRTGVRTIGIPGAMLRFDLQQGFPAVTTKKLAFKSAIGELVGFLRASRSAAEFRALGCKVW
DANANENAAWLANPYRTGVDDLGDVYGVQWRRWPGYKVLDAQATAQLEDALARGYRIVTRFDEAGAQKLLLHKAIDQLRD
CLDTIMRDPSSRRILFHGWNPVKLEEIALPACHLLYQFLPNLAKREISLCLYIRSNDVGLGTPFNLAEGAALLELVGRLT
GYQPRWFSYFIGDAHIYENQLDMLRQQLEREPYESPRLEIAERVPDYAKTGVYAPEWLERIEPADFTLAGYRHHAPLSAP
MAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=322, Percent_Identity=34.1614906832298, Blast_Score=186, Evalue=3e-47,
Organism=Escherichia coli, GI1789191, Length=323, Percent_Identity=34.0557275541796, Blast_Score=175, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI71993377, Length=324, Percent_Identity=32.4074074074074, Blast_Score=161, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6324648, Length=330, Percent_Identity=26.969696969697, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI17137556, Length=321, Percent_Identity=34.5794392523364, Blast_Score=192, Evalue=3e-49,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 36599; Mature: 36599

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLDLVRTILDTGSWQENRTGVRTIGIPGAMLRFDLQQGFPAVTTKKLAFKSAIGELV
CHHHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
GFLRASRSAAEFRALGCKVWDANANENAAWLANPYRTGVDDLGDVYGVQWRRWPGYKVLD
HHHHHCCHHHHHHHHCCEEEECCCCCCCEEECCCHHCCHHHHHHHHCCCEEECCCCEEEC
AQATAQLEDALARGYRIVTRFDEAGAQKLLLHKAIDQLRDCLDTIMRDPSSRRILFHGWN
HHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
PVKLEEIALPACHLLYQFLPNLAKREISLCLYIRSNDVGLGTPFNLAEGAALLELVGRLT
CCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHC
GYQPRWFSYFIGDAHIYENQLDMLRQQLEREPYESPRLEIAERVPDYAKTGVYAPEWLER
CCCCCHHHEECCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHCCCCCHHHHHH
IEPADFTLAGYRHHAPLSAPMAV
CCCCCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MKQYLDLVRTILDTGSWQENRTGVRTIGIPGAMLRFDLQQGFPAVTTKKLAFKSAIGELV
CHHHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
GFLRASRSAAEFRALGCKVWDANANENAAWLANPYRTGVDDLGDVYGVQWRRWPGYKVLD
HHHHHCCHHHHHHHHCCEEEECCCCCCCEEECCCHHCCHHHHHHHHCCCEEECCCCEEEC
AQATAQLEDALARGYRIVTRFDEAGAQKLLLHKAIDQLRDCLDTIMRDPSSRRILFHGWN
HHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
PVKLEEIALPACHLLYQFLPNLAKREISLCLYIRSNDVGLGTPFNLAEGAALLELVGRLT
CCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHC
GYQPRWFSYFIGDAHIYENQLDMLRQQLEREPYESPRLEIAERVPDYAKTGVYAPEWLER
CCCCCHHHEECCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHCCCCCHHHHHH
IEPADFTLAGYRHHAPLSAPMAV
CCCCCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]