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The map label for this gene is puuR [H]

Identifier: 238025512

GI number: 238025512

Start: 2740851

End: 2741432

Strand: Reverse

Name: puuR [H]

Synonym: bglu_2g22080

Alternate gene names: 238025512

Gene position: 2741432-2740851 (Counterclockwise)

Preceding gene: 238025515

Following gene: 238025511

Centisome position: 96.96

GC content: 65.12

Gene sequence:

>582_bases
ATGGTTGCTTCCACTGAAAATACGCAGCCCCGCAACGCGGCGGTCGAGCTGGGCAGCAAGATCCGTGCGCTCAGGCAGCG
GCTCAAGCGTACGCTCGACGAAACCGCGAGTGCCGCGGGCATTTCCAAGCCGTTCCTGTCGCAGGTCGAGCGCGGGCTGG
CATCGCCGTCGATCACCTCGCTGGCCGGCATCGCGCAGGCGCTCGGCGTGAACGTGCAGTACTTCGTCGAGACGCCGAGC
GAGGAGCGCTCGGTGTGCCGGGGCGAGCAGTTGCGTTTCTTCAGTTTTGCCGATTCGGCCAATCTGTTCGCACGGCTGAC
CAATGTGTCGGCCGGCCGGCAGCTCGAGGCGATCCTCGTGCGCATGCCGCCGGGACAGAAGCGCTCGGAAGTGACCACGC
ACGCGGGAGAGGAGTTCCTGTACGTGATCGAAGGGGAGGTATCGCTGACGCTGGAAGGCAAGACCTTCGTCGTGCAGGCC
GGCGACAGTGCGCATTACTTGTCGACCACGCCGCATAGCTGGGTCAACACCGCCAAGCACGAATCGGTGGTGGTCTGGGT
CGGCACGCCGCGCCTGTTCTAG

Upstream 100 bases:

>100_bases
ATATTGCAGTTACCGGTCACGAAGACGAAGATTACGGCATATTAGTCAAACTTCGTTACTTTCCATCAAACTTTTTTTGA
CGACAAGATCCCGGCCTGTT

Downstream 100 bases:

>100_bases
AAAATCGGACCGCCCGCGCTCGCATGCAGCCGGCGGTTCACGCGACAACAATTCAGGATAGCTAACATGAACGGCAGCGC
TCACGGACCGCCTCCGATTC

Product: MerR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MVASTENTQPRNAAVELGSKIRALRQRLKRTLDETASAAGISKPFLSQVERGLASPSITSLAGIAQALGVNVQYFVETPS
EERSVCRGEQLRFFSFADSANLFARLTNVSAGRQLEAILVRMPPGQKRSEVTTHAGEEFLYVIEGEVSLTLEGKTFVVQA
GDSAHYLSTTPHSWVNTAKHESVVVWVGTPRLF

Sequences:

>Translated_193_residues
MVASTENTQPRNAAVELGSKIRALRQRLKRTLDETASAAGISKPFLSQVERGLASPSITSLAGIAQALGVNVQYFVETPS
EERSVCRGEQLRFFSFADSANLFARLTNVSAGRQLEAILVRMPPGQKRSEVTTHAGEEFLYVIEGEVSLTLEGKTFVVQA
GDSAHYLSTTPHSWVNTAKHESVVVWVGTPRLF
>Mature_193_residues
MVASTENTQPRNAAVELGSKIRALRQRLKRTLDETASAAGISKPFLSQVERGLASPSITSLAGIAQALGVNVQYFVETPS
EERSVCRGEQLRFFSFADSANLFARLTNVSAGRQLEAILVRMPPGQKRSEVTTHAGEEFLYVIEGEVSLTLEGKTFVVQA
GDSAHYLSTTPHSWVNTAKHESVVVWVGTPRLF

Specific function: Represses puuD [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787557, Length=179, Percent_Identity=26.2569832402235, Blast_Score=67, Evalue=8e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013096
- InterPro:   IPR011051
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR014710 [H]

Pfam domain/function: PF07883 Cupin_2; PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 21034; Mature: 21034

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVASTENTQPRNAAVELGSKIRALRQRLKRTLDETASAAGISKPFLSQVERGLASPSITS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHH
LAGIAQALGVNVQYFVETPSEERSVCRGEQLRFFSFADSANLFARLTNVSAGRQLEAILV
HHHHHHHHCCCEEEEEECCCHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCCCEEEEEEE
RMPPGQKRSEVTTHAGEEFLYVIEGEVSLTLEGKTFVVQAGDSAHYLSTTPHSWVNTAKH
ECCCCCCCCHHHHCCCCEEEEEEECEEEEEECCCEEEEECCCCCEEECCCCHHHHHCCCC
ESVVVWVGTPRLF
CEEEEEECCCCCC
>Mature Secondary Structure
MVASTENTQPRNAAVELGSKIRALRQRLKRTLDETASAAGISKPFLSQVERGLASPSITS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHH
LAGIAQALGVNVQYFVETPSEERSVCRGEQLRFFSFADSANLFARLTNVSAGRQLEAILV
HHHHHHHHCCCEEEEEECCCHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCCCEEEEEEE
RMPPGQKRSEVTTHAGEEFLYVIEGEVSLTLEGKTFVVQAGDSAHYLSTTPHSWVNTAKH
ECCCCCCCCHHHHCCCCEEEEEEECEEEEEECCCEEEEECCCCCEEECCCCHHHHHCCCC
ESVVVWVGTPRLF
CEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553 [H]