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The map label for this gene is prpB [H]

Identifier: 238025479

GI number: 238025479

Start: 2696078

End: 2696971

Strand: Reverse

Name: prpB [H]

Synonym: bglu_2g21710

Alternate gene names: 238025479

Gene position: 2696971-2696078 (Counterclockwise)

Preceding gene: 238025482

Following gene: 238025478

Centisome position: 95.39

GC content: 67.9

Gene sequence:

>894_bases
ATGGGCCATTCCCAACTTTCCAGCGCCGGCGCCAAGTTCCGCGCCGCGGTCGCAGCCGAGGCGCCGCTGCAGGTGGTCGG
TGCGATCACCGCCTATGCGGCCAAGATGGCCGAGGCCACCGGCTTCAAGGCCGTCTATCTGTCGGGCGGCGGGGTGGCCG
CGAATTCGCTGGGCATGCCCGACCTCGGCATCAGCACCATGGAAGACGTGCTGATCGACGCAAACCGCATCACCAACGCC
ACCAGCCTGCCGCTGCTGGTGGACATCGACACCGGCTGGGGCGGCGCCTTCAACATCGCGCGCACCATCCGCTCGTTCAT
CAAGGCCGGGGTGGCCGCGGTCCACCTCGAGGACCAGGTCGGCCAGAAGCGCTGCGGGCACCGCCCGGGCAAGGAGTGCG
TGCCGGCCGAGGAGATGGTCGATCGCATCAAGGCCGCCGTGGACGCGCGCACCGACGACCAGTTCGTGATCATGGCGCGC
ACCGACGTGGCGGCCGCCGAAGGGCTCGACGCCGCGATCGAGCGCGCGGTGGCCTACGTCGAGGCCGGCGCCGACATGAT
CTTCCCCGAGGCCATGAAGACGCTCGACGACTACCGGCGCGTCAAGGAGGCGGTGAAGGTGCCGATCCTCGCGAACCTGA
CCGAGTTCGGCTCGACGCCGATGTTCACCATCGACGAGCTGAAGCGCGCCGACGTCGATATCGCGCTCTACTGCTGCGGC
GCGTATCGTGCGATGAACAAGGCGGCGCTGAACTTCTACGAAACGGTGCGCCGCGACGGCACGCAGAAGGCCGCCGTGCC
GACCATGCAGACGCGCGCGGAGCTGTACGAGTTTCTCGGCTACCACGCCTACGAGGACAAACTCGACGAACTGTTCAGCC
AGGGCAGGCACTGA

Upstream 100 bases:

>100_bases
TTTGGGTTGCGAATCAGCGGTGTAGCGGCGGGCGGCGGGCCTGGCCCGATCCTTGCTGCAGCCATGGCGACAACGTCATT
CGCCAGGAGGCAAGTTCGCG

Downstream 100 bases:

>100_bases
GCGGCGGATCGGTTCAGAATCCCGAATCACGGCCGGCGCCGACCGGAAACGGACCCGAAGACGGCCGAACGAACCCGAAG
AACGACCAGGAGAACATCAC

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MGHSQLSSAGAKFRAAVAAEAPLQVVGAITAYAAKMAEATGFKAVYLSGGGVAANSLGMPDLGISTMEDVLIDANRITNA
TSLPLLVDIDTGWGGAFNIARTIRSFIKAGVAAVHLEDQVGQKRCGHRPGKECVPAEEMVDRIKAAVDARTDDQFVIMAR
TDVAAAEGLDAAIERAVAYVEAGADMIFPEAMKTLDDYRRVKEAVKVPILANLTEFGSTPMFTIDELKRADVDIALYCCG
AYRAMNKAALNFYETVRRDGTQKAAVPTMQTRAELYEFLGYHAYEDKLDELFSQGRH

Sequences:

>Translated_297_residues
MGHSQLSSAGAKFRAAVAAEAPLQVVGAITAYAAKMAEATGFKAVYLSGGGVAANSLGMPDLGISTMEDVLIDANRITNA
TSLPLLVDIDTGWGGAFNIARTIRSFIKAGVAAVHLEDQVGQKRCGHRPGKECVPAEEMVDRIKAAVDARTDDQFVIMAR
TDVAAAEGLDAAIERAVAYVEAGADMIFPEAMKTLDDYRRVKEAVKVPILANLTEFGSTPMFTIDELKRADVDIALYCCG
AYRAMNKAALNFYETVRRDGTQKAAVPTMQTRAELYEFLGYHAYEDKLDELFSQGRH
>Mature_296_residues
GHSQLSSAGAKFRAAVAAEAPLQVVGAITAYAAKMAEATGFKAVYLSGGGVAANSLGMPDLGISTMEDVLIDANRITNAT
SLPLLVDIDTGWGGAFNIARTIRSFIKAGVAAVHLEDQVGQKRCGHRPGKECVPAEEMVDRIKAAVDARTDDQFVIMART
DVAAAEGLDAAIERAVAYVEAGADMIFPEAMKTLDDYRRVKEAVKVPILANLTEFGSTPMFTIDELKRADVDIALYCCGA
YRAMNKAALNFYETVRRDGTQKAAVPTMQTRAELYEFLGYHAYEDKLDELFSQGRH

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=292, Percent_Identity=63.013698630137, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI1790445, Length=259, Percent_Identity=27.7992277992278, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71982926, Length=231, Percent_Identity=29.004329004329, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17561814, Length=231, Percent_Identity=29.004329004329, Blast_Score=74, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6320908, Length=105, Percent_Identity=34.2857142857143, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 31980; Mature: 31849

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHSQLSSAGAKFRAAVAAEAPLQVVGAITAYAAKMAEATGFKAVYLSGGGVAANSLGMP
CCCHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCCC
DLGISTMEDVLIDANRITNATSLPLLVDIDTGWGGAFNIARTIRSFIKAGVAAVHLEDQV
CCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCHHEEEEHHHH
GQKRCGHRPGKECVPAEEMVDRIKAAVDARTDDQFVIMARTDVAAAEGLDAAIERAVAYV
HHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
EAGADMIFPEAMKTLDDYRRVKEAVKVPILANLTEFGSTPMFTIDELKRADVDIALYCCG
HCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEHHHHHHCCCCEEEEEHH
AYRAMNKAALNFYETVRRDGTQKAAVPTMQTRAELYEFLGYHAYEDKLDELFSQGRH
HHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GHSQLSSAGAKFRAAVAAEAPLQVVGAITAYAAKMAEATGFKAVYLSGGGVAANSLGMP
CCHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCCC
DLGISTMEDVLIDANRITNATSLPLLVDIDTGWGGAFNIARTIRSFIKAGVAAVHLEDQV
CCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCHHEEEEHHHH
GQKRCGHRPGKECVPAEEMVDRIKAAVDARTDDQFVIMARTDVAAAEGLDAAIERAVAYV
HHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
EAGADMIFPEAMKTLDDYRRVKEAVKVPILANLTEFGSTPMFTIDELKRADVDIALYCCG
HCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEHHHHHHCCCCEEEEEHH
AYRAMNKAALNFYETVRRDGTQKAAVPTMQTRAELYEFLGYHAYEDKLDELFSQGRH
HHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9325432 [H]