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The map label for this gene is bphA1 [H]

Identifier: 238025338

GI number: 238025338

Start: 2499364

End: 2500623

Strand: Reverse

Name: bphA1 [H]

Synonym: bglu_2g20120

Alternate gene names: 238025338

Gene position: 2500623-2499364 (Counterclockwise)

Preceding gene: 238025339

Following gene: 238025337

Centisome position: 88.44

GC content: 65.16

Gene sequence:

>1260_bases
TTGAACTCACCCATCGATATCGCACACGCGCTCGACTGGAAGGGGGACGGCGCGAGCCGGACACCGTTCGAGGCCTATAC
GAGCGATGCGCTTTACCAGCGCGAACTGGAGCGCTTGTTCTATTCGAAACACTGGTGCTACATCGGCCTGGAGGCGGAGG
TGGCGTCTCCCGGCGATTTCCAGCGCACCGAGGTCGGCGAGCGGTCGGTCGTGATGACGCGCACCGAGGACGGCGAGGTG
GTCGTGTTCGAGAACGCCTGCGCGCATCGCGGCATGCAGTTCTGCCGCGAGCGCGCGGGCAAAGCGAAGGACTTCCACTG
CCCGTATCACCAGTGGAACTACGACCTGAAGGGCAATCTGATCGGCGTGCCGTTTCGCCGTGGGGTACGGCAGAGCGGCA
AGATCAACGGCGGCATGCCGCCGGATTTCGATCCCAGGCATCACGCGTTGCGCCGGCTGCGGGTGTCGACGCGTGGCGGT
GCGGTGTTCGCGTCGTTCGACCAGGACGTCGAGTCCTTCGAGGATTTCCTCGGCCCCGACATCCTGCGCTATTACGACCG
CCTGTTCCACGGGCCGAAGCTGACCGTGCTCGGCTACACGCGCCAGCGAGTGCCGAGCAACTGGAAGCTGATGGTGGAGA
ACATCAAGGACCCGTATCACGCGGGACTGCTGCACACCTGGTTCGTCAACTTCGGGCTGTGGCGCGCGGACAACCCGGCC
CAGCTCGTCACCGACGCGCATCACCGCCACGGCGCGATGATCTCGATCCGCAGCGGCGGGGGCGGAGGCGAGGTCACGCA
CAACGTGACGAGCTTCAAGGAGCGCATGACGCTGAACGACGACCGCTTCCTCGACGTCGTGCACGAGCCGTGGTGGGGGG
AGCCGACCGTCGTGATGCTGACCCTGATGCCGAGCGTGATTATCCAGCAGCAGGTGAATGCGCTGTCGACGCGGCGCATC
CGCCCGGTCAGCCCGGGCGTGTTCGATTACGTATGGACGCATTTCGGCTTCGAGACCGACGACGATGACATGACGCGCCG
GCGGCTGCGCCAGGCAAACCTGTTCGGTCCTGCCGGCTTCGTCTCCGCGGACGACGGCGAGGTGATCGAATGCGTGCAGC
AGAGCTTCGAGCAGATCCGGCACGGCAACGTGTACAACGAGCTCGGCGGCCGCCAGGTCGACGAGCGCACCGACCATATG
GTCAGCGAAACGCTCGTGCGCGGCATGTACCAGTACTGGCGCCACGTGATGGAGGCATGA

Upstream 100 bases:

>100_bases
CCGCCGCGATTGCGTCGCGGCGGCACTCCCGAGTCGGCTTTCCGCTACCCGGCGTTCCGGCCGGCGCCGTGCAAGCCATT
TCTTACTGAAAGGAGCTTTC

Downstream 100 bases:

>100_bases
ATGACGATTTCATCCGTATTGGCCGACGCGCGGGTCGCGAGGCGCGGCACCGCAGCGGTGGCGGCGAGCTTCGCCGACTT
CTACGGCATCGTGGAGCTGA

Product: salicylate-5-hydroxylase large oxygenase component

Products: NA

Alternate protein names: Biphenyl 2,3-dioxygenase [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MNSPIDIAHALDWKGDGASRTPFEAYTSDALYQRELERLFYSKHWCYIGLEAEVASPGDFQRTEVGERSVVMTRTEDGEV
VVFENACAHRGMQFCRERAGKAKDFHCPYHQWNYDLKGNLIGVPFRRGVRQSGKINGGMPPDFDPRHHALRRLRVSTRGG
AVFASFDQDVESFEDFLGPDILRYYDRLFHGPKLTVLGYTRQRVPSNWKLMVENIKDPYHAGLLHTWFVNFGLWRADNPA
QLVTDAHHRHGAMISIRSGGGGGEVTHNVTSFKERMTLNDDRFLDVVHEPWWGEPTVVMLTLMPSVIIQQQVNALSTRRI
RPVSPGVFDYVWTHFGFETDDDDMTRRRLRQANLFGPAGFVSADDGEVIECVQQSFEQIRHGNVYNELGGRQVDERTDHM
VSETLVRGMYQYWRHVMEA

Sequences:

>Translated_419_residues
MNSPIDIAHALDWKGDGASRTPFEAYTSDALYQRELERLFYSKHWCYIGLEAEVASPGDFQRTEVGERSVVMTRTEDGEV
VVFENACAHRGMQFCRERAGKAKDFHCPYHQWNYDLKGNLIGVPFRRGVRQSGKINGGMPPDFDPRHHALRRLRVSTRGG
AVFASFDQDVESFEDFLGPDILRYYDRLFHGPKLTVLGYTRQRVPSNWKLMVENIKDPYHAGLLHTWFVNFGLWRADNPA
QLVTDAHHRHGAMISIRSGGGGGEVTHNVTSFKERMTLNDDRFLDVVHEPWWGEPTVVMLTLMPSVIIQQQVNALSTRRI
RPVSPGVFDYVWTHFGFETDDDDMTRRRLRQANLFGPAGFVSADDGEVIECVQQSFEQIRHGNVYNELGGRQVDERTDHM
VSETLVRGMYQYWRHVMEA
>Mature_419_residues
MNSPIDIAHALDWKGDGASRTPFEAYTSDALYQRELERLFYSKHWCYIGLEAEVASPGDFQRTEVGERSVVMTRTEDGEV
VVFENACAHRGMQFCRERAGKAKDFHCPYHQWNYDLKGNLIGVPFRRGVRQSGKINGGMPPDFDPRHHALRRLRVSTRGG
AVFASFDQDVESFEDFLGPDILRYYDRLFHGPKLTVLGYTRQRVPSNWKLMVENIKDPYHAGLLHTWFVNFGLWRADNPA
QLVTDAHHRHGAMISIRSGGGGGEVTHNVTSFKERMTLNDDRFLDVVHEPWWGEPTVVMLTLMPSVIIQQQVNALSTRRI
RPVSPGVFDYVWTHFGFETDDDDMTRRRLRQANLFGPAGFVSADDGEVIECVQQSFEQIRHGNVYNELGGRQVDERTDHM
VSETLVRGMYQYWRHVMEA

Specific function: Part Of The Multicomponent 3-Phenylpropionate Dioxygenase. Converts 3-Phenylpropionic Acid (Pp) Into Cis-3-(3- Carboxyethyl)-3,5-Cyclohexadiene-1,2-Diol (Pp-Dihydrodiol). [C]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=425, Percent_Identity=27.0588235294118, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1788103, Length=211, Percent_Identity=31.7535545023697, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.18 [H]

Molecular weight: Translated: 48142; Mature: 48142

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSPIDIAHALDWKGDGASRTPFEAYTSDALYQRELERLFYSKHWCYIGLEAEVASPGDF
CCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCC
QRTEVGERSVVMTRTEDGEVVVFENACAHRGMQFCRERAGKAKDFHCPYHQWNYDLKGNL
CHHCCCCCCEEEEECCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCE
IGVPFRRGVRQSGKINGGMPPDFDPRHHALRRLRVSTRGGAVFASFDQDVESFEDFLGPD
EECCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHCHH
ILRYYDRLFHGPKLTVLGYTRQRVPSNWKLMVENIKDPYHAGLLHTWFVNFGLWRADNPA
HHHHHHHHHCCCCEEEEECHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCH
QLVTDAHHRHGAMISIRSGGGGGEVTHNVTSFKERMTLNDDRFLDVVHEPWWGEPTVVML
HHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCCEEHH
TLMPSVIIQQQVNALSTRRIRPVSPGVFDYVWTHFGFETDDDDMTRRRLRQANLFGPAGF
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCC
VSADDGEVIECVQQSFEQIRHGNVYNELGGRQVDERTDHMVSETLVRGMYQYWRHVMEA
CCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNSPIDIAHALDWKGDGASRTPFEAYTSDALYQRELERLFYSKHWCYIGLEAEVASPGDF
CCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCC
QRTEVGERSVVMTRTEDGEVVVFENACAHRGMQFCRERAGKAKDFHCPYHQWNYDLKGNL
CHHCCCCCCEEEEECCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCE
IGVPFRRGVRQSGKINGGMPPDFDPRHHALRRLRVSTRGGAVFASFDQDVESFEDFLGPD
EECCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHCHH
ILRYYDRLFHGPKLTVLGYTRQRVPSNWKLMVENIKDPYHAGLLHTWFVNFGLWRADNPA
HHHHHHHHHCCCCEEEEECHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCH
QLVTDAHHRHGAMISIRSGGGGGEVTHNVTSFKERMTLNDDRFLDVVHEPWWGEPTVVML
HHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCCEEHH
TLMPSVIIQQQVNALSTRRIRPVSPGVFDYVWTHFGFETDDDDMTRRRLRQANLFGPAGF
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCC
VSADDGEVIECVQQSFEQIRHGNVYNELGGRQVDERTDHMVSETLVRGMYQYWRHVMEA
CCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8048958 [H]