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The map label for this gene is phaZ1 [H]

Identifier: 238024966

GI number: 238024966

Start: 2044326

End: 2045360

Strand: Reverse

Name: phaZ1 [H]

Synonym: bglu_2g16230

Alternate gene names: 238024966

Gene position: 2045360-2044326 (Counterclockwise)

Preceding gene: 238024968

Following gene: 238024965

Centisome position: 72.34

GC content: 71.98

Gene sequence:

>1035_bases
ATGCGATCTTTGCCGCCCCGCCTGCCACCGAAGCCGTGCCGCCTCGCGCGGCGAGCGCCGAGGGTGCGCCCCCATTTCCC
CTGGCCGCCGCGCGAGTCGCGCGTGCGGCCGCGCGCGGCCGCCTGGGCGGAGGGCGAGTGGTCGCGCGGCGAGCATCCGC
TGCCGTTCGTGGTCGGGCGCTTCGTGAGCCACCTCGGCTATGCGCTCTACGTGCCCGATGGGCTGCCCGCGGGGGCGCCG
CTCGTCGTGATGCTGCATGGCTGCAAGCAGGACGCCGCGCAGTTCGCGCAGGGCACGCGGATGAACCTGCTGGCCGATCG
TCATGGTTTCGCGGTGCTCTATCCGGAACAGTCGATCCAGGCGCATTCGCATGGCTGCTGGCACTGGTACGACGACACCG
AGCGCGGCGGACGCGGCGAGGCGCGTGCCGTGGTCGATCTGGTCGATACGCTGGTGGCGGCCCGCGGTCTCGACGCCTCG
CGCGTCTATGCCGCCGGCCTGTCGGCCGGAGCCGGGCTCGCCACGCTGCTGGCGCTGCATTTCCCCGACCGGTTCGCGGC
GATCGCGCTGCACTCGGGGCCGGCCTTCGGCGACGCGCATTCCGGCATCACCGCGATGGACGTGATGCGCCGCGGGCTGC
ATCGCGCACCGGCGGCGATTGCCGACAGCTACGCCGCGCCCGGAACCTATCCGGGCATGCCGGCGCTGATCGTGCACGGC
GACGACGACTCGGTGGTGTCGCCGGCCAATGCCGACCACCTCGCGATCGAATTCCTGCGCCTGAACGGCCTGGCCGACGC
CGACGGCGAGCCGAGCGGCGTCGAGCGGATCGACACGCAGGCTGGCGAGATCCGGCTGCTCGATTACCGGCGCGACGGCA
GGCCGGTGGTGCGGGTCTGCCATGTCGCCGGGCTGGATCACGCCTGGGCGGGCGGAGACGACACGGTGCCGTTCCATGCC
TCGGCCGGCCCCGACGCGAGCACGATGGTCTGGGGCTTTTTTGCGGATAAACAACGGCAGACTGCGACAATCTGA

Upstream 100 bases:

>100_bases
GCGAGCCGATCGATGACCAAGCGTCTGACCAAACTGTGGCTGGGCGGGATGAAGAAAATGCTGCGCACGCCAGCGTCGCG
GCTGCCGGCCGTCATCGCGG

Downstream 100 bases:

>100_bases
CGGTTTTTTTTGAAACGCTGGCGGCGCCTACGGGTTTTCCCTATAATTCGGGTTAATACCTAAGCGATAACCCTTGGATT
GGAGAATTCGTATGATGTAC

Product: polyhydroxybutyrate depolymerase domain-containing protein

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MRSLPPRLPPKPCRLARRAPRVRPHFPWPPRESRVRPRAAAWAEGEWSRGEHPLPFVVGRFVSHLGYALYVPDGLPAGAP
LVVMLHGCKQDAAQFAQGTRMNLLADRHGFAVLYPEQSIQAHSHGCWHWYDDTERGGRGEARAVVDLVDTLVAARGLDAS
RVYAAGLSAGAGLATLLALHFPDRFAAIALHSGPAFGDAHSGITAMDVMRRGLHRAPAAIADSYAAPGTYPGMPALIVHG
DDDSVVSPANADHLAIEFLRLNGLADADGEPSGVERIDTQAGEIRLLDYRRDGRPVVRVCHVAGLDHAWAGGDDTVPFHA
SAGPDASTMVWGFFADKQRQTATI

Sequences:

>Translated_344_residues
MRSLPPRLPPKPCRLARRAPRVRPHFPWPPRESRVRPRAAAWAEGEWSRGEHPLPFVVGRFVSHLGYALYVPDGLPAGAP
LVVMLHGCKQDAAQFAQGTRMNLLADRHGFAVLYPEQSIQAHSHGCWHWYDDTERGGRGEARAVVDLVDTLVAARGLDAS
RVYAAGLSAGAGLATLLALHFPDRFAAIALHSGPAFGDAHSGITAMDVMRRGLHRAPAAIADSYAAPGTYPGMPALIVHG
DDDSVVSPANADHLAIEFLRLNGLADADGEPSGVERIDTQAGEIRLLDYRRDGRPVVRVCHVAGLDHAWAGGDDTVPFHA
SAGPDASTMVWGFFADKQRQTATI
>Mature_344_residues
MRSLPPRLPPKPCRLARRAPRVRPHFPWPPRESRVRPRAAAWAEGEWSRGEHPLPFVVGRFVSHLGYALYVPDGLPAGAP
LVVMLHGCKQDAAQFAQGTRMNLLADRHGFAVLYPEQSIQAHSHGCWHWYDDTERGGRGEARAVVDLVDTLVAARGLDAS
RVYAAGLSAGAGLATLLALHFPDRFAAIALHSGPAFGDAHSGITAMDVMRRGLHRAPAAIADSYAAPGTYPGMPALIVHG
DDDSVVSPANADHLAIEFLRLNGLADADGEPSGVERIDTQAGEIRLLDYRRDGRPVVRVCHVAGLDHAWAGGDDTVPFHA
SAGPDASTMVWGFFADKQRQTATI

Specific function: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P- nitrophenylbutyrate as substrates [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 37041; Mature: 37041

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSLPPRLPPKPCRLARRAPRVRPHFPWPPRESRVRPRAAAWAEGEWSRGEHPLPFVVGR
CCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCHHHHHHH
FVSHLGYALYVPDGLPAGAPLVVMLHGCKQDAAQFAQGTRMNLLADRHGFAVLYPEQSIQ
HHHHCCEEEECCCCCCCCCCEEEEEECCHHHHHHHHCCCCEEEEECCCCEEEECCCHHHH
AHSHGCWHWYDDTERGGRGEARAVVDLVDTLVAARGLDASRVYAAGLSAGAGLATLLALH
HCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHH
FPDRFAAIALHSGPAFGDAHSGITAMDVMRRGLHRAPAAIADSYAAPGTYPGMPALIVHG
CCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCEEEEEC
DDDSVVSPANADHLAIEFLRLNGLADADGEPSGVERIDTQAGEIRLLDYRRDGRPVVRVC
CCCCEECCCCCCCEEEEEEEECCCCCCCCCCCCHHHHCCCCCCEEEEEECCCCCHHEEHH
HVAGLDHAWAGGDDTVPFHASAGPDASTMVWGFFADKQRQTATI
HHHCCCCCCCCCCCCCCEECCCCCCCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MRSLPPRLPPKPCRLARRAPRVRPHFPWPPRESRVRPRAAAWAEGEWSRGEHPLPFVVGR
CCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCHHHHHHH
FVSHLGYALYVPDGLPAGAPLVVMLHGCKQDAAQFAQGTRMNLLADRHGFAVLYPEQSIQ
HHHHCCEEEECCCCCCCCCCEEEEEECCHHHHHHHHCCCCEEEEECCCCEEEECCCHHHH
AHSHGCWHWYDDTERGGRGEARAVVDLVDTLVAARGLDASRVYAAGLSAGAGLATLLALH
HCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHH
FPDRFAAIALHSGPAFGDAHSGITAMDVMRRGLHRAPAAIADSYAAPGTYPGMPALIVHG
CCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCEEEEEC
DDDSVVSPANADHLAIEFLRLNGLADADGEPSGVERIDTQAGEIRLLDYRRDGRPVVRVC
CCCCEECCCCCCCEEEEEEEECCCCCCCCCCCCHHHHCCCCCCEEEEEECCCCCHHEEHH
HVAGLDHAWAGGDDTVPFHASAGPDASTMVWGFFADKQRQTATI
HHHCCCCCCCCCCCCCCEECCCCCCCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8269961 [H]