Click here to switch to the map view.

The map label for this gene is pyrB [H]

Identifier: 238024829

GI number: 238024829

Start: 1867449

End: 1868741

Strand: Direct

Name: pyrB [H]

Synonym: bglu_2g14690

Alternate gene names: 238024829

Gene position: 1867449-1868741 (Clockwise)

Preceding gene: 238024827

Following gene: 238024830

Centisome position: 66.05

GC content: 66.98

Gene sequence:

>1293_bases
ATGACCACGCCCCAGCAAGCCTTCCTGCGCGATGCGATGCGTCGCCTCAACCTGACCCGCGAAGCGTTCGCGAACCGCAT
CGGCGTGAGCCGCAGGGCACTCGATACCTGGCTGCTGCCCGACGACTCCCAGGAATCGCGCTCGATGCCCGAGATCGTCG
AACGCTATGTGACGGAAATCGTCGCGCGCACTTCGGAAGAAGCAAAATATACGCAAAGCGTAGATATCTCATCGCTTGCG
AGTCAGATCCAGTTCGAGGGGCGCCCGCAGCTGCTGTCCGTCGATCAGTTCTCGCGCGACTCGGTCGAAGCGCTGTTCCG
CGTGGCCGACGTGATGCAGCCGATCGCGCGCCGCCGCAAGATCTCGCGCGTGCTGGAGGGCGCCGTGCTCGGCAACCTGT
TCTTCGAGGCCAGCACGCGCACCCGCGTGTCGTTCGGCGCCGCGTTCTGCCGGCTCGGCGGCTCGGTCTGCGACACCACC
GGCTTCACGTTCTCGTCGATGGCCAAGGGCGAATCGATCCACGACACCGGCCGCGTGATGGCCGGCTATGTCGATGCGCT
CGTGATCCGCCATCCCGAACAGGGCTCGGTGGCCGAGTTCGCGCGCGCGACCAACCTGCCGGTGATCAACGGCGGCGACG
GCCCCGGCGAGCACCCGAGCCAGGCGCTGCTCGATCTCTACACGATCCAGCGCGAGTTCTCGCGGCTCGGCAAGATCGTC
GACGGCTCGCACATCGCGTTCGTCGGCGACCTGAAATACGGCCGCACCGTCCACTCGCTGGCCAAGCTGCTGGCGCTCTA
TCGCGGCATCCGCTTCACGCTGGTCGCGCCGGCCTCGCTGGAGATGCCGCGCTACATCGTCGAGCAGATCTCGAAGAACG
GCCACGTGATCGAGGAAACCACCGATCTGTCACGCGGGCTGGCCGGCGCGGACGTGGTCTACGCCACGCGGATCCAGAAG
GAGCGCTTCACCGACGAATCGTTCGAGGGCTACACGCCCGACTTCCAGATCAACCAGGCGCTCGTCGACGCCGTGTGCCA
GCGCGACACGCTGATCATGCACCCGCTGCCGCGCGACAGCCGGCCCGGCGCCAACGATCTCGCCACCGACCTGAACCGCG
ACCCGCGGCTGGCGATCTTCCGCCAGACCGACAACGGCATTCCGGTGCGCATGGCGGTGTTCGCCGTGCTGCTCGGCGTC
GATCATCTGGTGCAGCACTCGATGCGCGACGCGACCTGGCGGTCGCCCGTGCACCTTGGCCCCGAGGACGCGGTATTCCA
CGGGATCGACTGA

Upstream 100 bases:

>100_bases
GGCGGCGAAGGTACATCCAAGGCTGGCGAGCGCCGCGGCTTGACTTCGGCCCGGCGCCGCTTAATATACGCCTTGCGTAT
ATTTTGATACCGAACCTGCC

Downstream 100 bases:

>100_bases
CTGAAGGGCGCGGCACGGCCGGGGACGCCGCCGCGCGTGCGCTGGCGGCAAGACGCACACCGGCCGCGCTGCGATCGTCT
ATAACGGAAGCGCGCGGCTG

Product: aspartate carbamoyltransferase

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MTTPQQAFLRDAMRRLNLTREAFANRIGVSRRALDTWLLPDDSQESRSMPEIVERYVTEIVARTSEEAKYTQSVDISSLA
SQIQFEGRPQLLSVDQFSRDSVEALFRVADVMQPIARRRKISRVLEGAVLGNLFFEASTRTRVSFGAAFCRLGGSVCDTT
GFTFSSMAKGESIHDTGRVMAGYVDALVIRHPEQGSVAEFARATNLPVINGGDGPGEHPSQALLDLYTIQREFSRLGKIV
DGSHIAFVGDLKYGRTVHSLAKLLALYRGIRFTLVAPASLEMPRYIVEQISKNGHVIEETTDLSRGLAGADVVYATRIQK
ERFTDESFEGYTPDFQINQALVDAVCQRDTLIMHPLPRDSRPGANDLATDLNRDPRLAIFRQTDNGIPVRMAVFAVLLGV
DHLVQHSMRDATWRSPVHLGPEDAVFHGID

Sequences:

>Translated_430_residues
MTTPQQAFLRDAMRRLNLTREAFANRIGVSRRALDTWLLPDDSQESRSMPEIVERYVTEIVARTSEEAKYTQSVDISSLA
SQIQFEGRPQLLSVDQFSRDSVEALFRVADVMQPIARRRKISRVLEGAVLGNLFFEASTRTRVSFGAAFCRLGGSVCDTT
GFTFSSMAKGESIHDTGRVMAGYVDALVIRHPEQGSVAEFARATNLPVINGGDGPGEHPSQALLDLYTIQREFSRLGKIV
DGSHIAFVGDLKYGRTVHSLAKLLALYRGIRFTLVAPASLEMPRYIVEQISKNGHVIEETTDLSRGLAGADVVYATRIQK
ERFTDESFEGYTPDFQINQALVDAVCQRDTLIMHPLPRDSRPGANDLATDLNRDPRLAIFRQTDNGIPVRMAVFAVLLGV
DHLVQHSMRDATWRSPVHLGPEDAVFHGID
>Mature_429_residues
TTPQQAFLRDAMRRLNLTREAFANRIGVSRRALDTWLLPDDSQESRSMPEIVERYVTEIVARTSEEAKYTQSVDISSLAS
QIQFEGRPQLLSVDQFSRDSVEALFRVADVMQPIARRRKISRVLEGAVLGNLFFEASTRTRVSFGAAFCRLGGSVCDTTG
FTFSSMAKGESIHDTGRVMAGYVDALVIRHPEQGSVAEFARATNLPVINGGDGPGEHPSQALLDLYTIQREFSRLGKIVD
GSHIAFVGDLKYGRTVHSLAKLLALYRGIRFTLVAPASLEMPRYIVEQISKNGHVIEETTDLSRGLAGADVVYATRIQKE
RFTDESFEGYTPDFQINQALVDAVCQRDTLIMHPLPRDSRPGANDLATDLNRDPRLAIFRQTDNGIPVRMAVFAVLLGVD
HLVQHSMRDATWRSPVHLGPEDAVFHGID

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=311, Percent_Identity=41.8006430868167, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI38788445, Length=331, Percent_Identity=27.7945619335347, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI2367364, Length=318, Percent_Identity=37.1069182389937, Blast_Score=211, Evalue=6e-56,
Organism=Escherichia coli, GI1790703, Length=277, Percent_Identity=26.7148014440433, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1786469, Length=281, Percent_Identity=27.0462633451957, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI193204318, Length=332, Percent_Identity=42.7710843373494, Blast_Score=233, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6322331, Length=391, Percent_Identity=36.5728900255754, Blast_Score=228, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6322373, Length=302, Percent_Identity=25.4966887417219, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24642586, Length=318, Percent_Identity=40.251572327044, Blast_Score=228, Evalue=9e-60,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 47779; Mature: 47648

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPQQAFLRDAMRRLNLTREAFANRIGVSRRALDTWLLPDDSQESRSMPEIVERYVTEI
CCCHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHH
VARTSEEAKYTQSVDISSLASQIQFEGRPQLLSVDQFSRDSVEALFRVADVMQPIARRRK
HHHCCHHHHHHHCCCHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
ISRVLEGAVLGNLFFEASTRTRVSFGAAFCRLGGSVCDTTGFTFSSMAKGESIHDTGRVM
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHCCCCCCHHHHHCCCCHHHHHHHH
AGYVDALVIRHPEQGSVAEFARATNLPVINGGDGPGEHPSQALLDLYTIQREFSRLGKIV
HHHHHHHHEECCCCCCHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
DGSHIAFVGDLKYGRTVHSLAKLLALYRGIRFTLVAPASLEMPRYIVEQISKNGHVIEET
CCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCEEECH
TDLSRGLAGADVVYATRIQKERFTDESFEGYTPDFQINQALVDAVCQRDTLIMHPLPRDS
HHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCC
RPGANDLATDLNRDPRLAIFRQTDNGIPVRMAVFAVLLGVDHLVQHSMRDATWRSPVHLG
CCCCHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PEDAVFHGID
CCCCCCCCCC
>Mature Secondary Structure 
TTPQQAFLRDAMRRLNLTREAFANRIGVSRRALDTWLLPDDSQESRSMPEIVERYVTEI
CCHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHH
VARTSEEAKYTQSVDISSLASQIQFEGRPQLLSVDQFSRDSVEALFRVADVMQPIARRRK
HHHCCHHHHHHHCCCHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
ISRVLEGAVLGNLFFEASTRTRVSFGAAFCRLGGSVCDTTGFTFSSMAKGESIHDTGRVM
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHCCCCCCHHHHHCCCCHHHHHHHH
AGYVDALVIRHPEQGSVAEFARATNLPVINGGDGPGEHPSQALLDLYTIQREFSRLGKIV
HHHHHHHHEECCCCCCHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
DGSHIAFVGDLKYGRTVHSLAKLLALYRGIRFTLVAPASLEMPRYIVEQISKNGHVIEET
CCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCEEECH
TDLSRGLAGADVVYATRIQKERFTDESFEGYTPDFQINQALVDAVCQRDTLIMHPLPRDS
HHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCC
RPGANDLATDLNRDPRLAIFRQTDNGIPVRMAVFAVLLGVDHLVQHSMRDATWRSPVHLG
CCCCHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PEDAVFHGID
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA