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The map label for this gene is pycA [H]

Identifier: 238024679

GI number: 238024679

Start: 1684119

End: 1685576

Strand: Direct

Name: pycA [H]

Synonym: bglu_2g13080

Alternate gene names: 238024679

Gene position: 1684119-1685576 (Clockwise)

Preceding gene: 238024678

Following gene: 238024680

Centisome position: 59.57

GC content: 69.41

Gene sequence:

>1458_bases
ATGAAGCCGGCAACCTCCCCCGACGCGTTCTATCGTCCGTCGCGGATCACGACCGTGCTGGTCGCGAATCGCGGCGAGAT
CGCGGTGCGCGTGATCCGCGCGGCGCGCGAACTCGGCATGCGCGCGGTCGCCGTGGTCAGCGACGCCGACCGCGACAGCC
TCGCCGCGCGCATGGCCGACGAGGCGATCCGCATCGGCGCATCGCATGCCGCGAAAAGCTATCTAAATCCCGCCGTGATC
CTCGACGCTGCGCGGCAATGCGGTGCGGACGCGATCCATCCCGGCTACGGCTTCCTGTCCGAAAATGCCGCGTTCGCCGC
GCAGGTCGAGGCGGCAGGGCTGATCTTCGTCGGGCCGGATTCGCAGGTCATTGCGACGATGGGCGACAAGGCGCGCGCCC
GTGACACCGCGCGGCGCGCGAACGTGCCAACGGTGCCCGGCAGCGACGGCGTCGTGCAGTCGCTCGACGAGGCCCGCGCA
GTCGCCGGGCGAATCGGTTATCCAATTATGATCAAGGCGGCCGCGGGCGGCGGCGGGCGCGGCATTCGCGTTGCGCACGA
CGCCGCACAGCTCGACGCCGAGCTGCCGCTCGCGCAGCGCGAGGCGCAGGCCGCGTTCGGCGACGGCGGCGTCTATCTCG
AACGCTTCATCGCGTGCGCCCGGCACATCGAGGTGCAGGTGCTCGGCGACGGCCACGACGTCGTCCATCTGTTCGAGCGC
GAATGCTCGCTGCAGCGACGCCGCCAGAAGATTCTCGAGGAAGCGCCGTCGCCGTCGCTCACGCCGGAGCAGCGCGACGC
TCTGTGCACGTCGGCCACGCGCCTCGCGCGCGAAGTCGGCTATCGCAGTGCGGGCACGCTCGAATACCTGTTCGACGACG
CGCGCGGCGAATTCTACTTCATCGAGATGAACACGCGCATCCAGGTTGAGCATCCAGTAACCGAGGCGATCACCGGCATC
GACCTCGTGCGCGAGACGCTGCGCATCGCCGACGGCGAGCCGCTGCGGTTCGAGCAAACGGACATCGCGATACGGGGCGC
GGCGCTCGAATGCCGGATCAATGCGGAAGATCCGCTGCAGGATTTCCGCCCGAATCCAGGCCGGATCGACGCACTCGTAT
GGCCGACCGGCCCCGGCGTACGCATCGATTCGCTGCTCTACCCGGGCTACGTCGTGCCGCCGTTCTACGATTCGCTGCTC
GCGAAGCTAATCGTGCACGACGAGAGCCGCGGGGCCGCGCTGCGGCGTCTCGCTCGCGCGCTCGGCGAGTTGCACGTCGG
CGGCCTGAAGACCACCGCGCCGCTGCACGTCGCGCTGCTCGCGGACGACGACGTCCGCGCGGGCCGTTATCACACGAATT
TTCTCGAAGCTTGGATGCTGCGATGGCGCGAGGCCGCAACGGCCGCCGCCCGGCAGCATGAAACCGCGGACGCCGCGCGC
GTCGGGGAGGCACTGTGA

Upstream 100 bases:

>100_bases
ATCGAAGCGGACGCGGCCGGCCGCGTCGTCGAGCTGCTCGTCGACGACGGCGAACCGGTCGACGCGGGCCAGGTACTGAT
GCGGATCGAGGGGTGACGCG

Downstream 100 bases:

>100_bases
CGATCCGTTATACGTTCGGCGGCGACGAGTTCGTGTTCGCCGAGATCAGCGAGGCGATGTCGCTCGATGCCTTCTTCGAG
GGCGCGGCGATCACGCGCGA

Product: acetyl-CoA carboxylase biotin carboxylase subunit

Products: NA

Alternate protein names: Pyruvic carboxylase A [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MKPATSPDAFYRPSRITTVLVANRGEIAVRVIRAARELGMRAVAVVSDADRDSLAARMADEAIRIGASHAAKSYLNPAVI
LDAARQCGADAIHPGYGFLSENAAFAAQVEAAGLIFVGPDSQVIATMGDKARARDTARRANVPTVPGSDGVVQSLDEARA
VAGRIGYPIMIKAAAGGGGRGIRVAHDAAQLDAELPLAQREAQAAFGDGGVYLERFIACARHIEVQVLGDGHDVVHLFER
ECSLQRRRQKILEEAPSPSLTPEQRDALCTSATRLAREVGYRSAGTLEYLFDDARGEFYFIEMNTRIQVEHPVTEAITGI
DLVRETLRIADGEPLRFEQTDIAIRGAALECRINAEDPLQDFRPNPGRIDALVWPTGPGVRIDSLLYPGYVVPPFYDSLL
AKLIVHDESRGAALRRLARALGELHVGGLKTTAPLHVALLADDDVRAGRYHTNFLEAWMLRWREAATAAARQHETADAAR
VGEAL

Sequences:

>Translated_485_residues
MKPATSPDAFYRPSRITTVLVANRGEIAVRVIRAARELGMRAVAVVSDADRDSLAARMADEAIRIGASHAAKSYLNPAVI
LDAARQCGADAIHPGYGFLSENAAFAAQVEAAGLIFVGPDSQVIATMGDKARARDTARRANVPTVPGSDGVVQSLDEARA
VAGRIGYPIMIKAAAGGGGRGIRVAHDAAQLDAELPLAQREAQAAFGDGGVYLERFIACARHIEVQVLGDGHDVVHLFER
ECSLQRRRQKILEEAPSPSLTPEQRDALCTSATRLAREVGYRSAGTLEYLFDDARGEFYFIEMNTRIQVEHPVTEAITGI
DLVRETLRIADGEPLRFEQTDIAIRGAALECRINAEDPLQDFRPNPGRIDALVWPTGPGVRIDSLLYPGYVVPPFYDSLL
AKLIVHDESRGAALRRLARALGELHVGGLKTTAPLHVALLADDDVRAGRYHTNFLEAWMLRWREAATAAARQHETADAAR
VGEAL
>Mature_485_residues
MKPATSPDAFYRPSRITTVLVANRGEIAVRVIRAARELGMRAVAVVSDADRDSLAARMADEAIRIGASHAAKSYLNPAVI
LDAARQCGADAIHPGYGFLSENAAFAAQVEAAGLIFVGPDSQVIATMGDKARARDTARRANVPTVPGSDGVVQSLDEARA
VAGRIGYPIMIKAAAGGGGRGIRVAHDAAQLDAELPLAQREAQAAFGDGGVYLERFIACARHIEVQVLGDGHDVVHLFER
ECSLQRRRQKILEEAPSPSLTPEQRDALCTSATRLAREVGYRSAGTLEYLFDDARGEFYFIEMNTRIQVEHPVTEAITGI
DLVRETLRIADGEPLRFEQTDIAIRGAALECRINAEDPLQDFRPNPGRIDALVWPTGPGVRIDSLLYPGYVVPPFYDSLL
AKLIVHDESRGAALRRLARALGELHVGGLKTTAPLHVALLADDDVRAGRYHTNFLEAWMLRWREAATAAARQHETADAAR
VGEAL

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG0439

COG function: function code I; Biotin carboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 biotin carboxylation domain [H]

Homologues:

Organism=Homo sapiens, GI65506442, Length=450, Percent_Identity=47.7777777777778, Blast_Score=397, Evalue=1e-110,
Organism=Homo sapiens, GI295821183, Length=450, Percent_Identity=47.7777777777778, Blast_Score=397, Evalue=1e-110,
Organism=Homo sapiens, GI189095269, Length=440, Percent_Identity=48.1818181818182, Blast_Score=396, Evalue=1e-110,
Organism=Homo sapiens, GI116805327, Length=443, Percent_Identity=45.5981941309255, Blast_Score=376, Evalue=1e-104,
Organism=Homo sapiens, GI106049528, Length=463, Percent_Identity=44.9244060475162, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI106049295, Length=463, Percent_Identity=44.9244060475162, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI106049292, Length=463, Percent_Identity=44.9244060475162, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI38679977, Length=503, Percent_Identity=31.610337972167, Blast_Score=231, Evalue=9e-61,
Organism=Homo sapiens, GI38679967, Length=503, Percent_Identity=31.610337972167, Blast_Score=231, Evalue=9e-61,
Organism=Homo sapiens, GI38679974, Length=503, Percent_Identity=31.610337972167, Blast_Score=231, Evalue=1e-60,
Organism=Homo sapiens, GI38679971, Length=503, Percent_Identity=31.610337972167, Blast_Score=231, Evalue=1e-60,
Organism=Homo sapiens, GI38679960, Length=503, Percent_Identity=31.610337972167, Blast_Score=231, Evalue=1e-60,
Organism=Homo sapiens, GI134142062, Length=504, Percent_Identity=31.547619047619, Blast_Score=218, Evalue=7e-57,
Organism=Escherichia coli, GI1789654, Length=446, Percent_Identity=50.4484304932735, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1786216, Length=244, Percent_Identity=25, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17567343, Length=448, Percent_Identity=46.6517857142857, Blast_Score=383, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71987519, Length=454, Percent_Identity=46.9162995594714, Blast_Score=380, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17562816, Length=451, Percent_Identity=44.1241685144124, Blast_Score=355, Evalue=3e-98,
Organism=Caenorhabditis elegans, GI133931226, Length=486, Percent_Identity=29.2181069958848, Blast_Score=203, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI71997168, Length=509, Percent_Identity=28.4872298624754, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI71997163, Length=509, Percent_Identity=28.4872298624754, Blast_Score=187, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6319685, Length=442, Percent_Identity=43.2126696832579, Blast_Score=377, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6319695, Length=457, Percent_Identity=44.6389496717724, Blast_Score=356, Evalue=6e-99,
Organism=Saccharomyces cerevisiae, GI6321376, Length=489, Percent_Identity=43.1492842535787, Blast_Score=352, Evalue=7e-98,
Organism=Saccharomyces cerevisiae, GI6324343, Length=511, Percent_Identity=31.3111545988258, Blast_Score=220, Evalue=4e-58,
Organism=Saccharomyces cerevisiae, GI6323863, Length=469, Percent_Identity=30.4904051172708, Blast_Score=213, Evalue=7e-56,
Organism=Drosophila melanogaster, GI24651757, Length=455, Percent_Identity=45.0549450549451, Blast_Score=377, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24652212, Length=462, Percent_Identity=45.6709956709957, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24652210, Length=462, Percent_Identity=45.6709956709957, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24652214, Length=462, Percent_Identity=45.6709956709957, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI19921944, Length=462, Percent_Identity=45.6709956709957, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24652216, Length=462, Percent_Identity=45.6709956709957, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI281363050, Length=462, Percent_Identity=45.6709956709957, Blast_Score=374, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24652224, Length=462, Percent_Identity=45.6709956709957, Blast_Score=374, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24652222, Length=462, Percent_Identity=45.6709956709957, Blast_Score=374, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24652220, Length=462, Percent_Identity=45.6709956709957, Blast_Score=374, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24652218, Length=462, Percent_Identity=45.6709956709957, Blast_Score=374, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24651759, Length=405, Percent_Identity=43.7037037037037, Blast_Score=330, Evalue=2e-90,
Organism=Drosophila melanogaster, GI161076407, Length=526, Percent_Identity=31.1787072243346, Blast_Score=225, Evalue=6e-59,
Organism=Drosophila melanogaster, GI24586460, Length=526, Percent_Identity=31.1787072243346, Blast_Score=225, Evalue=6e-59,
Organism=Drosophila melanogaster, GI24586458, Length=526, Percent_Identity=31.1787072243346, Blast_Score=225, Evalue=6e-59,
Organism=Drosophila melanogaster, GI161076409, Length=526, Percent_Identity=31.1787072243346, Blast_Score=224, Evalue=7e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004549
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011764
- InterPro:   IPR005482
- InterPro:   IPR005479
- InterPro:   IPR005481
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02785 Biotin_carb_C; PF00289 CPSase_L_chain; PF02786 CPSase_L_D2 [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 52470; Mature: 52470

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50975 ATP_GRASP ; PS00866 CPSASE_1 ; PS00867 CPSASE_2 ; PS50979 BC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPATSPDAFYRPSRITTVLVANRGEIAVRVIRAARELGMRAVAVVSDADRDSLAARMAD
CCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHH
EAIRIGASHAAKSYLNPAVILDAARQCGADAIHPGYGFLSENAAFAAQVEAAGLIFVGPD
HHHHHCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECEEEEECCC
SQVIATMGDKARARDTARRANVPTVPGSDGVVQSLDEARAVAGRIGYPIMIKAAAGGGGR
CCEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
GIRVAHDAAQLDAELPLAQREAQAAFGDGGVYLERFIACARHIEVQVLGDGHDVVHLFER
CEEEECHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCEEEEEECCCCHHHHHHHH
ECSLQRRRQKILEEAPSPSLTPEQRDALCTSATRLAREVGYRSAGTLEYLFDDARGEFYF
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHEEEECCCCCEEE
IEMNTRIQVEHPVTEAITGIDLVRETLRIADGEPLRFEQTDIAIRGAALECRINAEDPLQ
EEECCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEEECEEEEEEECCCCCHH
DFRPNPGRIDALVWPTGPGVRIDSLLYPGYVVPPFYDSLLAKLIVHDESRGAALRRLARA
HCCCCCCCEEEEEECCCCCCEEEEEECCCEECCHHHHHHHHHHHHCCCCCCHHHHHHHHH
LGELHVGGLKTTAPLHVALLADDDVRAGRYHTNFLEAWMLRWREAATAAARQHETADAAR
HHHHCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGEAL
HCCCC
>Mature Secondary Structure
MKPATSPDAFYRPSRITTVLVANRGEIAVRVIRAARELGMRAVAVVSDADRDSLAARMAD
CCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHH
EAIRIGASHAAKSYLNPAVILDAARQCGADAIHPGYGFLSENAAFAAQVEAAGLIFVGPD
HHHHHCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECEEEEECCC
SQVIATMGDKARARDTARRANVPTVPGSDGVVQSLDEARAVAGRIGYPIMIKAAAGGGGR
CCEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
GIRVAHDAAQLDAELPLAQREAQAAFGDGGVYLERFIACARHIEVQVLGDGHDVVHLFER
CEEEECHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCEEEEEECCCCHHHHHHHH
ECSLQRRRQKILEEAPSPSLTPEQRDALCTSATRLAREVGYRSAGTLEYLFDDARGEFYF
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHEEEECCCCCEEE
IEMNTRIQVEHPVTEAITGIDLVRETLRIADGEPLRFEQTDIAIRGAALECRINAEDPLQ
EEECCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEEECEEEEEEECCCCCHH
DFRPNPGRIDALVWPTGPGVRIDSLLYPGYVVPPFYDSLLAKLIVHDESRGAALRRLARA
HCCCCCCCEEEEEECCCCCCEEEEEECCCEECCHHHHHHHHHHHHCCCCCCHHHHHHHHH
LGELHVGGLKTTAPLHVALLADDDVRAGRYHTNFLEAWMLRWREAATAAARQHETADAAR
HHHHCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGEAL
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463; 9478969 [H]