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The map label for this gene is tnpR [H]

Identifier: 23501960

GI number: 23501960

Start: 1053748

End: 1054365

Strand: Reverse

Name: tnpR [H]

Synonym: BR1082

Alternate gene names: 23501960

Gene position: 1054365-1053748 (Counterclockwise)

Preceding gene: 23501961

Following gene: 23501959

Centisome position: 50.02

GC content: 58.09

Gene sequence:

>618_bases
ATGAACAATGCAACCGTCGCCAACGTCGCCGCTACCTACCGGGGTCAGCTTGTGGGGTACGCTCGTACATCGACAACCGA
TCAGAAGGCAGGACTTGCCGCACAAGAGCGAGACCTTCAAGCGGCAGGGTGTGATCGCATATTCAGTGAACAGACCTCTG
CCAAGGATAGTAAGAGGCCGGAACTGCAACGCGCCCTCGATCATCTGCGAGAAGGCGACACGTTCATTGTTACCAAGCTG
GACCGTCTGGCCCGTTCGGTTGCTGACTTGGTTGATCTCACCGGGCAACTCAGCAGCAAGGGCGTCAGTTTGCGTATCCT
CGCCATGAACCTCGACACGGCAACGCCGACAGGGAAGCTTATGCTGAACCTTTTGGGATCGATTGCGGAGTTCGAGCGGG
AACTGATGCTGGAACGCCAGCGGGAAGGTATTGCAGCGGCCAAGGCAGGCGGCAAGTACAAGGGCAGGGCACCCACAGCA
CAGCGTCAGGCAGACGAGATTATCCGCTTGAAGGACGAAGGGAAGACCGTCGCGGAAATCATGGCGGCAACCAAGGCGGG
GCGGTCTAGTGTATTCAGCATTCTTAAGGAGGCAGGAAGGACGGAGCGCCAGCGTTGA

Upstream 100 bases:

>100_bases
ATGGCGTATGCAGCAATAAAGTCCAATTAGAGTGTGTATTGACTTTATGGACTATTAGTCCGATAACTCCTATAAACCTA
TTTGGATTATGGGGGCCAAC

Downstream 100 bases:

>100_bases
CCGGAAGCAACGCAACGCTGTTCAGGACATGCTTGCCATGGGAGCCGGTACATCTGAGATTGCGAAGGCGACGGGATTGA
CACGACAGACCGTTATCAGG

Product: resolvase family site-specific recombinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MNNATVANVAATYRGQLVGYARTSTTDQKAGLAAQERDLQAAGCDRIFSEQTSAKDSKRPELQRALDHLREGDTFIVTKL
DRLARSVADLVDLTGQLSSKGVSLRILAMNLDTATPTGKLMLNLLGSIAEFERELMLERQREGIAAAKAGGKYKGRAPTA
QRQADEIIRLKDEGKTVAEIMAATKAGRSSVFSILKEAGRTERQR

Sequences:

>Translated_205_residues
MNNATVANVAATYRGQLVGYARTSTTDQKAGLAAQERDLQAAGCDRIFSEQTSAKDSKRPELQRALDHLREGDTFIVTKL
DRLARSVADLVDLTGQLSSKGVSLRILAMNLDTATPTGKLMLNLLGSIAEFERELMLERQREGIAAAKAGGKYKGRAPTA
QRQADEIIRLKDEGKTVAEIMAATKAGRSSVFSILKEAGRTERQR
>Mature_205_residues
MNNATVANVAATYRGQLVGYARTSTTDQKAGLAAQERDLQAAGCDRIFSEQTSAKDSKRPELQRALDHLREGDTFIVTKL
DRLARSVADLVDLTGQLSSKGVSLRILAMNLDTATPTGKLMLNLLGSIAEFERELMLERQREGIAAAKAGGKYKGRAPTA
QRQADEIIRLKDEGKTVAEIMAATKAGRSSVFSILKEAGRTERQR

Specific function: Resolvase catalyzes the resolution (a site-specific recombination) of the cointegrated replicon to yield the final transposition products [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=142, Percent_Identity=43.6619718309859, Blast_Score=124, Evalue=3e-30,
Organism=Escherichia coli, GI1787638, Length=186, Percent_Identity=34.9462365591398, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1787827, Length=186, Percent_Identity=35.4838709677419, Blast_Score=105, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 22280; Mature: 22280

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNATVANVAATYRGQLVGYARTSTTDQKAGLAAQERDLQAAGCDRIFSEQTSAKDSKRP
CCCCHHHHHHHHHHHHEEEEECCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCH
ELQRALDHLREGDTFIVTKLDRLARSVADLVDLTGQLSSKGVSLRILAMNLDTATPTGKL
HHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHH
MLNLLGSIAEFERELMLERQREGIAAAKAGGKYKGRAPTAQRQADEIIRLKDEGKTVAEI
HHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHH
MAATKAGRSSVFSILKEAGRTERQR
HHHHHCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNNATVANVAATYRGQLVGYARTSTTDQKAGLAAQERDLQAAGCDRIFSEQTSAKDSKRP
CCCCHHHHHHHHHHHHEEEEECCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCH
ELQRALDHLREGDTFIVTKLDRLARSVADLVDLTGQLSSKGVSLRILAMNLDTATPTGKL
HHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHH
MLNLLGSIAEFERELMLERQREGIAAAKAGGKYKGRAPTAQRQADEIIRLKDEGKTVAEI
HHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHH
MAATKAGRSSVFSILKEAGRTERQR
HHHHHCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2170815; 2555186 [H]