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The map label for this gene is glnA [H]

Identifier: 23501884

GI number: 23501884

Start: 967120

End: 968529

Strand: Reverse

Name: glnA [H]

Synonym: BR1004

Alternate gene names: 23501884

Gene position: 968529-967120 (Counterclockwise)

Preceding gene: 23501885

Following gene: 23501879

Centisome position: 45.95

GC content: 55.53

Gene sequence:

>1410_bases
ATGACGACTGCCAACGATATTCTGAAACAGATCAAAGATAACGACGTCAAGTTCGTCGACCTTCGCTTCACCGATCCGAA
GGGCAAGCTCCATCACGTTACGATGGATATCGGCCTCGTGGACGAAGACATGTTCGTCGACGGTGTCATGTTCGACGGCT
CTTCGATCGCAGGCTGGAAGGCCATCAACGAATCCGACATGGTTTTGATGCCCGATCCGGAAACGGCTCATATCGACCCG
TTCTTCGCCCAGTCCACGCTTGTCATTCTCTGCGATATCCTTGAGCCGCTTTCCGGCGAAAGCTACAGCCGTGATCCGCG
TACGACTGCCAAGAAGGCCGAAGCCTATCTGAAATCGCTCGGCATTGGCGATACGGTCTATGTCGGCCCGGAAGCCGAAT
TCTTCGTCTTCGACGATGTGAAGTTCAAGGCCGATCCGTTCAATACGGGCTTCAAGCTCGATAGCGCCGAATTGCCATCC
AACGACGACACCGATTATGAAACCGGCAATCTCGGCCATCGTCCGCGCGTCAAGGGCGGCTATTTCCCCGTTCCGCCAAT
CGACAGCTTGCAGGACATGCGTTCCGAAATGCTGACCGTGCTCTCGGAAATGGGTGTGACGGTTGAAAAGCACCACCATG
AAGTCGCTTCCGCGCAGCACGAACTGGGCACGAAATTCGACACGCTGGTGCGCAACGCCGACAAGATGCAGATTCAGAAA
TATGTCGTGCATCAGGTTGCCAATGCCTATGGCAAAACGGCAACCTTCATGCCGAAGCCGGTCTTTGGCGACAATGGCTC
CGGCATGCATGTTCACTTCTCGATCTGGAAAGACGGCAAGCCGACCTTCGCAGGCAATGAATATGCTGGTCTGTCGGAAA
ACTGCCTCTACTTCATTGGCGGCGTCATCAAGCACGCAAAAGCTGTGAATGCCTTCACCAACCCGTCCACCAACTCCTAC
AAGCGTCTGGTGCCGGGCTATGAAGCGCCTGTTCTGCTGGCCTATTCCGCACGCAACCGTTCGGCTTCCTGCCGCATTCC
ATTCGGCTCCTCGCCGAAATCGAAGCGTCTCGAAGTCCGCTTCCCGGATCCGTCCGCCAACCCTTATCTCTGCTTCTCGG
CCCTTCTGATGGCGGGCCTTGACGGCATCAAGAACAAGATCCATCCGGGCCAGGCCATGGACAAGGATCTCTACGACCTG
CCGCCGAAGGAACTGAAGCAGATTCCGACCGTCTGCGGCTCCCTGCGTGAAGCTCTACAGTCGCTCGACAAGGATCGTGA
ATTCCTCAAGGCTGGCGGCGTGTTCTCCGACGACCAGATCGACAGCTTCATCGAGCTGAAAATGGCTGAAGTGATGCGTT
TTGAAATGACGCCGCACCCGGTCGAGTTCGATATGTATTACTCGGTATAA

Upstream 100 bases:

>100_bases
TGAAAACAAACAGATAGAGCCTTTCCAATCACTTACATGAAACAGGAAATGCTCTAACCGAACCTGCTACCAATCATCGT
CGTGCGAACAGGAATTTAAA

Downstream 100 bases:

>100_bases
AGCTATAACGACCCATTACCGAGGGGAACGACGCGCTAAAAAGCGCGCGCAAAAGAGAAACCCCGGCTTTCGCCGGGGTT
TCTTCATTTTATCAATAAGT

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase I; Glutamine synthetase I; GSI [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTTANDILKQIKDNDVKFVDLRFTDPKGKLHHVTMDIGLVDEDMFVDGVMFDGSSIAGWKAINESDMVLMPDPETAHIDP
FFAQSTLVILCDILEPLSGESYSRDPRTTAKKAEAYLKSLGIGDTVYVGPEAEFFVFDDVKFKADPFNTGFKLDSAELPS
NDDTDYETGNLGHRPRVKGGYFPVPPIDSLQDMRSEMLTVLSEMGVTVEKHHHEVASAQHELGTKFDTLVRNADKMQIQK
YVVHQVANAYGKTATFMPKPVFGDNGSGMHVHFSIWKDGKPTFAGNEYAGLSENCLYFIGGVIKHAKAVNAFTNPSTNSY
KRLVPGYEAPVLLAYSARNRSASCRIPFGSSPKSKRLEVRFPDPSANPYLCFSALLMAGLDGIKNKIHPGQAMDKDLYDL
PPKELKQIPTVCGSLREALQSLDKDREFLKAGGVFSDDQIDSFIELKMAEVMRFEMTPHPVEFDMYYSV

Sequences:

>Translated_469_residues
MTTANDILKQIKDNDVKFVDLRFTDPKGKLHHVTMDIGLVDEDMFVDGVMFDGSSIAGWKAINESDMVLMPDPETAHIDP
FFAQSTLVILCDILEPLSGESYSRDPRTTAKKAEAYLKSLGIGDTVYVGPEAEFFVFDDVKFKADPFNTGFKLDSAELPS
NDDTDYETGNLGHRPRVKGGYFPVPPIDSLQDMRSEMLTVLSEMGVTVEKHHHEVASAQHELGTKFDTLVRNADKMQIQK
YVVHQVANAYGKTATFMPKPVFGDNGSGMHVHFSIWKDGKPTFAGNEYAGLSENCLYFIGGVIKHAKAVNAFTNPSTNSY
KRLVPGYEAPVLLAYSARNRSASCRIPFGSSPKSKRLEVRFPDPSANPYLCFSALLMAGLDGIKNKIHPGQAMDKDLYDL
PPKELKQIPTVCGSLREALQSLDKDREFLKAGGVFSDDQIDSFIELKMAEVMRFEMTPHPVEFDMYYSV
>Mature_468_residues
TTANDILKQIKDNDVKFVDLRFTDPKGKLHHVTMDIGLVDEDMFVDGVMFDGSSIAGWKAINESDMVLMPDPETAHIDPF
FAQSTLVILCDILEPLSGESYSRDPRTTAKKAEAYLKSLGIGDTVYVGPEAEFFVFDDVKFKADPFNTGFKLDSAELPSN
DDTDYETGNLGHRPRVKGGYFPVPPIDSLQDMRSEMLTVLSEMGVTVEKHHHEVASAQHELGTKFDTLVRNADKMQIQKY
VVHQVANAYGKTATFMPKPVFGDNGSGMHVHFSIWKDGKPTFAGNEYAGLSENCLYFIGGVIKHAKAVNAFTNPSTNSYK
RLVPGYEAPVLLAYSARNRSASCRIPFGSSPKSKRLEVRFPDPSANPYLCFSALLMAGLDGIKNKIHPGQAMDKDLYDLP
PKELKQIPTVCGSLREALQSLDKDREFLKAGGVFSDDQIDSFIELKMAEVMRFEMTPHPVEFDMYYSV

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=468, Percent_Identity=66.4529914529915, Blast_Score=656, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=315, Percent_Identity=25.7142857142857, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52161; Mature: 52030

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTANDILKQIKDNDVKFVDLRFTDPKGKLHHVTMDIGLVDEDMFVDGVMFDGSSIAGWK
CCCHHHHHHHHCCCCEEEEEEEEECCCCCEEEEEEEEEEECCCCEEEEEEECCCCCCCCE
AINESDMVLMPDPETAHIDPFFAQSTLVILCDILEPLSGESYSRDPRTTAKKAEAYLKSL
ECCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHC
GIGDTVYVGPEAEFFVFDDVKFKADPFNTGFKLDSAELPSNDDTDYETGNLGHRPRVKGG
CCCCEEEECCCCCEEEEECCEEECCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCC
YFPVPPIDSLQDMRSEMLTVLSEMGVTVEKHHHEVASAQHELGTKFDTLVRNADKMQIQK
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHH
YVVHQVANAYGKTATFMPKPVFGDNGSGMHVHFSIWKDGKPTFAGNEYAGLSENCLYFIG
HHHHHHHHHHCCCEECCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHH
GVIKHAKAVNAFTNPSTNSYKRLVPGYEAPVLLAYSARNRSASCRIPFGSSPKSKRLEVR
HHHHHHHHHHCCCCCCCCCHHHCCCCCCCCEEEEEECCCCCCEEECCCCCCCCCCEEEEE
FPDPSANPYLCFSALLMAGLDGIKNKIHPGQAMDKDLYDLPPKELKQIPTVCGSLREALQ
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHH
SLDKDREFLKAGGVFSDDQIDSFIELKMAEVMRFEMTPHPVEFDMYYSV
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECC
>Mature Secondary Structure 
TTANDILKQIKDNDVKFVDLRFTDPKGKLHHVTMDIGLVDEDMFVDGVMFDGSSIAGWK
CCHHHHHHHHCCCCEEEEEEEEECCCCCEEEEEEEEEEECCCCEEEEEEECCCCCCCCE
AINESDMVLMPDPETAHIDPFFAQSTLVILCDILEPLSGESYSRDPRTTAKKAEAYLKSL
ECCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHC
GIGDTVYVGPEAEFFVFDDVKFKADPFNTGFKLDSAELPSNDDTDYETGNLGHRPRVKGG
CCCCEEEECCCCCEEEEECCEEECCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCC
YFPVPPIDSLQDMRSEMLTVLSEMGVTVEKHHHEVASAQHELGTKFDTLVRNADKMQIQK
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHH
YVVHQVANAYGKTATFMPKPVFGDNGSGMHVHFSIWKDGKPTFAGNEYAGLSENCLYFIG
HHHHHHHHHHCCCEECCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHH
GVIKHAKAVNAFTNPSTNSYKRLVPGYEAPVLLAYSARNRSASCRIPFGSSPKSKRLEVR
HHHHHHHHHHCCCCCCCCCHHHCCCCCCCCEEEEEECCCCCCEEECCCCCCCCCCEEEEE
FPDPSANPYLCFSALLMAGLDGIKNKIHPGQAMDKDLYDLPPKELKQIPTVCGSLREALQ
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHH
SLDKDREFLKAGGVFSDDQIDSFIELKMAEVMRFEMTPHPVEFDMYYSV
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2882467 [H]