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The map label for this gene is artJ [H]

Identifier: 23501838

GI number: 23501838

Start: 926796

End: 927602

Strand: Reverse

Name: artJ [H]

Synonym: BR0955

Alternate gene names: 23501838

Gene position: 927602-926796 (Counterclockwise)

Preceding gene: 23501839

Following gene: 23501837

Centisome position: 44.01

GC content: 55.27

Gene sequence:

>807_bases
ATGCGCGTATTGAAGCGTATCGCTATGGCGACATCGGTTGCAGCCTTGGCAATCACGGTTGGCAGCATGGCTGCGGGCGT
CGCCAATGCAGAAGAACCGCTCAAGCTCACAATCGGCACCGAAGGCGCCTATCCTCCTTTCAATTTCATCAAGCCGGATG
GCACGCTATCGGGCTTCGACGTTGACATTGCCCGCGCGCTCTGTGACCAAATGAAAGCCAAGTGCGAGTTCGTGACCCAG
GACTGGGATGGCGCAATTCCAGCACTTCAGTCCGGCAAGTTCGATGCCTTCATCGCCTCCATGTCCATCACGGACGAGCG
CAAGAAGCAGGTGGACTTCACCAATAAATATTACAATACGCCACCCGGCATCGCCGCTCCGAAGGATACGGATATCAAGG
GCGTAACCAAGGAAGACCTTGCAGGCAAGACCATCGGTGTGCAGGGTTCCACGACGCACGCCAACTATTCGGAAAAGACC
TATACCGACAGCACCATCAAGTCCTATCCGACAGCGGAAGAATATCGCCTTGATCTTGCCAATGGCCGCCTTGATGCCGT
CAATGATGACAGTGTTACGCTTGCTGAATGGCTGAAGTCACCGGATGGCGCCTGCTGCAAGATGGTCGGCACCTTCCCGC
CCGTGACGGAAATTCATGGCCCGGGCGCAGGCATCGCCATCAAGAAGGGCCGCCCGGAACTTGTCGCGAAATTCAACGCC
GCGATCAAGGCAATCCGGGCCAACGGCAAGTATAAGGAAATCAACGATAAATATTTCGACTTCGATGCTTACGGTTCCGA
AAACTGA

Upstream 100 bases:

>100_bases
CGTGCAATTTGAAGATTTCACGGTCGTTATTGCCCTACTAAACGGCTGAACCAGAACAAAAAATCAGGGAACCAGCCAGA
ACAAAACGAGAGGACTGAAA

Downstream 100 bases:

>100_bases
TCATCAACGACCTTTGTGCGCCGGTTTGCTTCCAGGCCAGCCGGCGCATGGAATTTTGCATGAGCGCCCGACAGGCCGTA
TGTCTAATTCCAGGAACAGC

Product: amino acid ABC transporter periplasmic amino acid-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MRVLKRIAMATSVAALAITVGSMAAGVANAEEPLKLTIGTEGAYPPFNFIKPDGTLSGFDVDIARALCDQMKAKCEFVTQ
DWDGAIPALQSGKFDAFIASMSITDERKKQVDFTNKYYNTPPGIAAPKDTDIKGVTKEDLAGKTIGVQGSTTHANYSEKT
YTDSTIKSYPTAEEYRLDLANGRLDAVNDDSVTLAEWLKSPDGACCKMVGTFPPVTEIHGPGAGIAIKKGRPELVAKFNA
AIKAIRANGKYKEINDKYFDFDAYGSEN

Sequences:

>Translated_268_residues
MRVLKRIAMATSVAALAITVGSMAAGVANAEEPLKLTIGTEGAYPPFNFIKPDGTLSGFDVDIARALCDQMKAKCEFVTQ
DWDGAIPALQSGKFDAFIASMSITDERKKQVDFTNKYYNTPPGIAAPKDTDIKGVTKEDLAGKTIGVQGSTTHANYSEKT
YTDSTIKSYPTAEEYRLDLANGRLDAVNDDSVTLAEWLKSPDGACCKMVGTFPPVTEIHGPGAGIAIKKGRPELVAKFNA
AIKAIRANGKYKEINDKYFDFDAYGSEN
>Mature_268_residues
MRVLKRIAMATSVAALAITVGSMAAGVANAEEPLKLTIGTEGAYPPFNFIKPDGTLSGFDVDIARALCDQMKAKCEFVTQ
DWDGAIPALQSGKFDAFIASMSITDERKKQVDFTNKYYNTPPGIAAPKDTDIKGVTKEDLAGKTIGVQGSTTHANYSEKT
YTDSTIKSYPTAEEYRLDLANGRLDAVNDDSVTLAEWLKSPDGACCKMVGTFPPVTEIHGPGAGIAIKKGRPELVAKFNA
AIKAIRANGKYKEINDKYFDFDAYGSEN

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=245, Percent_Identity=38.7755102040816, Blast_Score=167, Evalue=6e-43,
Organism=Escherichia coli, GI1788649, Length=260, Percent_Identity=35.3846153846154, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1787088, Length=256, Percent_Identity=34.375, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI1788648, Length=236, Percent_Identity=36.0169491525424, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1787031, Length=249, Percent_Identity=32.1285140562249, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1788228, Length=258, Percent_Identity=29.8449612403101, Blast_Score=113, Evalue=1e-26,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28857; Mature: 28857

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLKRIAMATSVAALAITVGSMAAGVANAEEPLKLTIGTEGAYPPFNFIKPDGTLSGFD
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCH
VDIARALCDQMKAKCEFVTQDWDGAIPALQSGKFDAFIASMSITDERKKQVDFTNKYYNT
HHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCEEEEEEECCCHHHHHHCCHHHCCCCC
PPGIAAPKDTDIKGVTKEDLAGKTIGVQGSTTHANYSEKTYTDSTIKSYPTAEEYRLDLA
CCCCCCCCCCCCCCCCHHHCCCCEEECCCCCCCCCCCCCCCCCCHHHCCCCHHHEEEEEC
NGRLDAVNDDSVTLAEWLKSPDGACCKMVGTFPPVTEIHGPGAGIAIKKGRPELVAKFNA
CCEEECCCCCCCHHHHHHCCCCCCCEEEECCCCCHHHCCCCCCCEEEECCCHHHHHHHHH
AIKAIRANGKYKEINDKYFDFDAYGSEN
HHHHHHCCCCEEECCCCEEEECCCCCCC
>Mature Secondary Structure
MRVLKRIAMATSVAALAITVGSMAAGVANAEEPLKLTIGTEGAYPPFNFIKPDGTLSGFD
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCH
VDIARALCDQMKAKCEFVTQDWDGAIPALQSGKFDAFIASMSITDERKKQVDFTNKYYNT
HHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCEEEEEEECCCHHHHHHCCHHHCCCCC
PPGIAAPKDTDIKGVTKEDLAGKTIGVQGSTTHANYSEKTYTDSTIKSYPTAEEYRLDLA
CCCCCCCCCCCCCCCCHHHCCCCEEECCCCCCCCCCCCCCCCCCHHHCCCCHHHEEEEEC
NGRLDAVNDDSVTLAEWLKSPDGACCKMVGTFPPVTEIHGPGAGIAIKKGRPELVAKFNA
CCEEECCCCCCCHHHHHHCCCCCCCEEEECCCCCHHHCCCCCCCEEEECCCHHHHHHHHH
AIKAIRANGKYKEINDKYFDFDAYGSEN
HHHHHHCCCCEEECCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]