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The map label for this gene is iscA [H]

Identifier: 23501823

GI number: 23501823

Start: 913556

End: 913936

Strand: Reverse

Name: iscA [H]

Synonym: BR0939

Alternate gene names: 23501823

Gene position: 913936-913556 (Counterclockwise)

Preceding gene: 23501833

Following gene: 23501820

Centisome position: 43.36

GC content: 57.48

Gene sequence:

>381_bases
ATGGGCCGTTTTGCAGTCATAACGCTTACAGACGCCGCGGCAAACCGCGTCCACGAGATCATGACCTCGCGTGAAGATGC
GCTTGGGATCCGCGTCGGCGTGAAAAAGGGCGGCTGTGCAGGTATGGAATATACGATCGAACTCGTCACCGAGGCAAAAG
CCGGCGACGACCTGATCGAAAAGGACGGCGCGAAAGTTTTCATTGCCCCGGAAGCCGTTCTTTTTCTTCTGGGAACGCAG
ATGGATTTTGAAGTCACAACGCTGCGCACGGGGTTTGTCTTCAACAACCCCAACCAGACCTCCGCCTGCGGCTGTGGCGA
ATCCGTCGAGTTGAAACCCGCCTCGCCAGAAAGCCTGCGCCAGATGCAGGGTACAGCCTGA

Upstream 100 bases:

>100_bases
CTATAGTGAAAACGTGGCAAAAGTGGTTTTAAGCCGCTTGCCTCACAGCAGACTGGATGATTCTCCAGCCATATGCCAGC
CAGTACCGGAAGAGTTTGAG

Downstream 100 bases:

>100_bases
TTCCGCCAAACGGCGCTACGCAGACGGACGACGAAACGCTCCGCGAGCTTCTCTGCATGGGCTTGAGCCAAGCCTTACTG
GAATATTCCCGCGAAAACCT

Product: HesB/YadR/YfhF family protein

Products: NA

Alternate protein names: Iron-sulfur cluster assembly protein [H]

Number of amino acids: Translated: 126; Mature: 125

Protein sequence:

>126_residues
MGRFAVITLTDAAANRVHEIMTSREDALGIRVGVKKGGCAGMEYTIELVTEAKAGDDLIEKDGAKVFIAPEAVLFLLGTQ
MDFEVTTLRTGFVFNNPNQTSACGCGESVELKPASPESLRQMQGTA

Sequences:

>Translated_126_residues
MGRFAVITLTDAAANRVHEIMTSREDALGIRVGVKKGGCAGMEYTIELVTEAKAGDDLIEKDGAKVFIAPEAVLFLLGTQ
MDFEVTTLRTGFVFNNPNQTSACGCGESVELKPASPESLRQMQGTA
>Mature_125_residues
GRFAVITLTDAAANRVHEIMTSREDALGIRVGVKKGGCAGMEYTIELVTEAKAGDDLIEKDGAKVFIAPEAVLFLLGTQM
DFEVTTLRTGFVFNNPNQTSACGCGESVELKPASPESLRQMQGTA

Specific function: Is able to transfer iron-sulfur clusters to apo- ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that iscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transi

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesB/iscA family [H]

Homologues:

Organism=Homo sapiens, GI52426768, Length=107, Percent_Identity=41.1214953271028, Blast_Score=99, Evalue=8e-22,
Organism=Homo sapiens, GI160420328, Length=107, Percent_Identity=35.5140186915888, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI1788877, Length=105, Percent_Identity=43.8095238095238, Blast_Score=96, Evalue=9e-22,
Organism=Escherichia coli, GI1787974, Length=102, Percent_Identity=43.1372549019608, Blast_Score=91, Evalue=2e-20,
Organism=Escherichia coli, GI1786351, Length=106, Percent_Identity=33.0188679245283, Blast_Score=72, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI193209025, Length=107, Percent_Identity=41.1214953271028, Blast_Score=102, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI25146411, Length=107, Percent_Identity=41.1214953271028, Blast_Score=102, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6323001, Length=104, Percent_Identity=37.5, Blast_Score=86, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24642263, Length=107, Percent_Identity=43.9252336448598, Blast_Score=102, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000361
- InterPro:   IPR016092
- InterPro:   IPR017870
- InterPro:   IPR011302 [H]

Pfam domain/function: PF01521 Fe-S_biosyn [H]

EC number: NA

Molecular weight: Translated: 13428; Mature: 13296

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRFAVITLTDAAANRVHEIMTSREDALGIRVGVKKGGCAGMEYTIELVTEAKAGDDLIE
CCCEEEEEEECHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEEECCCCCCCHHH
KDGAKVFIAPEAVLFLLGTQMDFEVTTLRTGFVFNNPNQTSACGCGESVELKPASPESLR
CCCCEEEECCCEEEEEECCCCCEEEEEEEEEEEEECCCCCCCCCCCCCEEECCCCHHHHH
QMQGTA
HHCCCC
>Mature Secondary Structure 
GRFAVITLTDAAANRVHEIMTSREDALGIRVGVKKGGCAGMEYTIELVTEAKAGDDLIE
CCEEEEEEECHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEEECCCCCCCHHH
KDGAKVFIAPEAVLFLLGTQMDFEVTTLRTGFVFNNPNQTSACGCGESVELKPASPESLR
CCCCEEEECCCEEEEEECCCCCEEEEEEEEEEEEECCCCCCCCCCCCCEEECCCCHHHHH
QMQGTA
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA