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The map label for this gene is trmFO [H]

Identifier: 23501780

GI number: 23501780

Start: 867369

End: 868769

Strand: Reverse

Name: trmFO [H]

Synonym: BR0894

Alternate gene names: 23501780

Gene position: 868769-867369 (Counterclockwise)

Preceding gene: 23501781

Following gene: 23501775

Centisome position: 41.22

GC content: 59.81

Gene sequence:

>1401_bases
ATGTCAAACAATACCGATCTCTCCCCCGTTCACGTCATTGGCGGCGGCCTTGCCGGCAGCGAAGCCGCCTGGCAGATCGC
ACAGGCCGGCGTACCGGTCGTGCTGCACGAAATGCGCCCGGTTCGCGGCACCGATGCGCACAAGACCGAACAGCTTGCAG
AGCTCGTCTGCTCCAACTCCTTCCGCTCCGACGACGCAGAAACCAACGCCGTCGGTGTTCTCCATGCCGAGATGCGCCTT
GCCGGATCGCTCATCATGGCTTGCGCGGATGCCCATCAGGTTCCCGCTGGCGGTGCGCTGGCCGTTGACCGTGAAGGCTT
TTCGCAGGCCGTAACCGCCAGACTGGAAGCCCATCCGCTCATCACCATCGAGCGCGAGGAGATCACCGGCCTGCCGCCGA
CCGAATGGGGAACGACGATCATCGCCACCGGCCCGCTGACCGCACCGTCGCTGGCCGAAGCCATCGCCGCTGAAACCGAT
GCGGATGCGCTTGCCTTTTTCGATGCAATTGCGCCCATCATTCATTTCGATTCAATCAATATGGATGTGTGCTGGTTCCA
GTCGCGCTATGACAAGGTTGGTCCCGGCGGTACTGGGAAAGATTATATCAACTGCCCGATGGACAAGGAGCAGTACGAAG
CCTTCGTCGCCGCCCTCATCGAAGGCGACAAGACGGACTTCAAGGAATGGGAAGGCACGCCCTATTTCGATGGTTGCCTG
CCGATCGAGGTGATGGCTGAGCGCGGACCGGAAACGCTGCGTCACGGCCCGATGAAACCGATGGGCCTTACCAATGCGCA
TAACCCGACTGTGAAGCCCTATGCGGTCGTGCAGTTGCGACAGGACAATGCGCTTGGCACGCTCTACAACATGGTGGGTT
TCCAGACGAAGCTGAAATATGGCTCGCAGACGGGCATTTTTAAAATGATACCCGGCCTGGAGAACGCGGAATTTGCACGC
CTTGGCGGCCTGCACCGCAACACCTACCTTAACTCTCCCGTATTGCTCGACAATGTTCTGCGTCTCAAATCCCGTCAGAC
ATTGCGTTTTGCAGGTCAGGTAACGGGTTGTGAGGGCTATGTCGAATCGTCGGCCATCGGCCTGCTGGCCGGTCGCTTTA
CGGCTGCGGAAAAGCTTAGTCAGGCTGCCGTGCCGCCCCCGCCCACGACGGCTTTCGGCGCGCTGCTTGGCCATATTACC
GGTGGCCATATCGTTACCGATGACGAACCCGGCAAGCGTTCCTTCCAGCCGATGAACGTGAATTTCGGCCTGTTTCCGCC
CGTTGACGTACCCAAACCCGAAGGCAAGCGCCTGCGCGGCAAGGAAAAGACCATTGCCAAAAAGCGCGCGCTTTCCGCAC
GCGCCCTTGCCGATTGCCGAAACTGGCTCAGCTTGTACTGA

Upstream 100 bases:

>100_bases
TCCTCCCGCGACGGATGCCATTTCTTTTTGCATCTCCCTTCCCGGTTTCTGGTCCCTTCTCCCATTGAAGGCGCTGATCA
AAGGCGATAAGGGAATTGCC

Downstream 100 bases:

>100_bases
AGAAAATGGGGCTTCGGCGCCTTTGCCGGAGCCGCTTCATGATGCGCGGTTTCAAAAATTTGCGCGGAACATCAGCCATT
GTTTACGGATGGCAGAAATA

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MSNNTDLSPVHVIGGGLAGSEAAWQIAQAGVPVVLHEMRPVRGTDAHKTEQLAELVCSNSFRSDDAETNAVGVLHAEMRL
AGSLIMACADAHQVPAGGALAVDREGFSQAVTARLEAHPLITIEREEITGLPPTEWGTTIIATGPLTAPSLAEAIAAETD
ADALAFFDAIAPIIHFDSINMDVCWFQSRYDKVGPGGTGKDYINCPMDKEQYEAFVAALIEGDKTDFKEWEGTPYFDGCL
PIEVMAERGPETLRHGPMKPMGLTNAHNPTVKPYAVVQLRQDNALGTLYNMVGFQTKLKYGSQTGIFKMIPGLENAEFAR
LGGLHRNTYLNSPVLLDNVLRLKSRQTLRFAGQVTGCEGYVESSAIGLLAGRFTAAEKLSQAAVPPPPTTAFGALLGHIT
GGHIVTDDEPGKRSFQPMNVNFGLFPPVDVPKPEGKRLRGKEKTIAKKRALSARALADCRNWLSLY

Sequences:

>Translated_466_residues
MSNNTDLSPVHVIGGGLAGSEAAWQIAQAGVPVVLHEMRPVRGTDAHKTEQLAELVCSNSFRSDDAETNAVGVLHAEMRL
AGSLIMACADAHQVPAGGALAVDREGFSQAVTARLEAHPLITIEREEITGLPPTEWGTTIIATGPLTAPSLAEAIAAETD
ADALAFFDAIAPIIHFDSINMDVCWFQSRYDKVGPGGTGKDYINCPMDKEQYEAFVAALIEGDKTDFKEWEGTPYFDGCL
PIEVMAERGPETLRHGPMKPMGLTNAHNPTVKPYAVVQLRQDNALGTLYNMVGFQTKLKYGSQTGIFKMIPGLENAEFAR
LGGLHRNTYLNSPVLLDNVLRLKSRQTLRFAGQVTGCEGYVESSAIGLLAGRFTAAEKLSQAAVPPPPTTAFGALLGHIT
GGHIVTDDEPGKRSFQPMNVNFGLFPPVDVPKPEGKRLRGKEKTIAKKRALSARALADCRNWLSLY
>Mature_465_residues
SNNTDLSPVHVIGGGLAGSEAAWQIAQAGVPVVLHEMRPVRGTDAHKTEQLAELVCSNSFRSDDAETNAVGVLHAEMRLA
GSLIMACADAHQVPAGGALAVDREGFSQAVTARLEAHPLITIEREEITGLPPTEWGTTIIATGPLTAPSLAEAIAAETDA
DALAFFDAIAPIIHFDSINMDVCWFQSRYDKVGPGGTGKDYINCPMDKEQYEAFVAALIEGDKTDFKEWEGTPYFDGCLP
IEVMAERGPETLRHGPMKPMGLTNAHNPTVKPYAVVQLRQDNALGTLYNMVGFQTKLKYGSQTGIFKMIPGLENAEFARL
GGLHRNTYLNSPVLLDNVLRLKSRQTLRFAGQVTGCEGYVESSAIGLLAGRFTAAEKLSQAAVPPPPTTAFGALLGHITG
GHIVTDDEPGKRSFQPMNVNFGLFPPVDVPKPEGKRLRGKEKTIAKKRALSARALADCRNWLSLY

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: =2.1.1.74 [H]

Molecular weight: Translated: 50176; Mature: 50045

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNTDLSPVHVIGGGLAGSEAAWQIAQAGVPVVLHEMRPVRGTDAHKTEQLAELVCSNS
CCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCC
FRSDDAETNAVGVLHAEMRLAGSLIMACADAHQVPAGGALAVDREGFSQAVTARLEAHPL
CCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHCCCCE
ITIEREEITGLPPTEWGTTIIATGPLTAPSLAEAIAAETDADALAFFDAIAPIIHFDSIN
EEEEHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHEECCCC
MDVCWFQSRYDKVGPGGTGKDYINCPMDKEQYEAFVAALIEGDKTDFKEWEGTPYFDGCL
CEEEEEHHHCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCC
PIEVMAERGPETLRHGPMKPMGLTNAHNPTVKPYAVVQLRQDNALGTLYNMVGFQTKLKY
CHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCC
GSQTGIFKMIPGLENAEFARLGGLHRNTYLNSPVLLDNVLRLKSRQTLRFAGQVTGCEGY
CCCCCCEEECCCCCCCCHHHHCCCHHCCCCCCCHHHHHHHHHHCCCCEEECCEECCCCCH
VESSAIGLLAGRFTAAEKLSQAAVPPPPTTAFGALLGHITGGHIVTDDEPGKRSFQPMNV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCC
NFGLFPPVDVPKPEGKRLRGKEKTIAKKRALSARALADCRNWLSLY
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNNTDLSPVHVIGGGLAGSEAAWQIAQAGVPVVLHEMRPVRGTDAHKTEQLAELVCSNS
CCCCCCCEEEEEECCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCC
FRSDDAETNAVGVLHAEMRLAGSLIMACADAHQVPAGGALAVDREGFSQAVTARLEAHPL
CCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHCCCCE
ITIEREEITGLPPTEWGTTIIATGPLTAPSLAEAIAAETDADALAFFDAIAPIIHFDSIN
EEEEHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHEECCCC
MDVCWFQSRYDKVGPGGTGKDYINCPMDKEQYEAFVAALIEGDKTDFKEWEGTPYFDGCL
CEEEEEHHHCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCC
PIEVMAERGPETLRHGPMKPMGLTNAHNPTVKPYAVVQLRQDNALGTLYNMVGFQTKLKY
CHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCC
GSQTGIFKMIPGLENAEFARLGGLHRNTYLNSPVLLDNVLRLKSRQTLRFAGQVTGCEGY
CCCCCCEEECCCCCCCCHHHHCCCHHCCCCCCCHHHHHHHHHHCCCCEEECCEECCCCCH
VESSAIGLLAGRFTAAEKLSQAAVPPPPTTAFGALLGHITGGHIVTDDEPGKRSFQPMNV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCC
NFGLFPPVDVPKPEGKRLRGKEKTIAKKRALSARALADCRNWLSLY
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA