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The map label for this gene is xth-1 [H]

Identifier: 23501759

GI number: 23501759

Start: 844306

End: 845088

Strand: Reverse

Name: xth-1 [H]

Synonym: BR0873

Alternate gene names: 23501759

Gene position: 845088-844306 (Counterclockwise)

Preceding gene: 23501761

Following gene: 23501756

Centisome position: 40.09

GC content: 54.92

Gene sequence:

>783_bases
ATGAAAATCGCTACATGGAACATCAACGGCGTCAAGGCGCGCATCGACAATCTCCAGCATTGGCTGAGGGAGTCGTCGCC
CGACATCGTCTGCCTGCAGGAAATCAAGTCGGTGGATGAGCAATTTCCCCGCCTTGAGATCGAAGCACTCGGCTATCATG
TCGAAACCCACGGCCAGAAGGGTTTCAATGGGGTGGCGCTGCTCTCAAAAAAATCACCGGATGAGATCAATAAAGGCCTT
CCCGGTGACGATAGCGACGAACAGGCCCGTTTCATCGAAGGCATTTATTCGACCGATACTGGTGTCGTGCGGGTCGTGTC
GCTCTATTTGCCCAACGGCAATCCGATCGACACCGAAAAGTTCCCCTACAAGCTTTCATGGATGCAGCGGCTTGAAAATT
GGGCAAAGAAGCGCCTGACACTGGAAGAACCATTGGTTCTGGCGGGTGACTATAACGTCATTCCCGAACCCGTGGACGCC
CGCAACCCGCAGGCATGGCTGGGCGATGCGCTGTTTCAGCCGCAATCCCGGCAGGCATTCCGCAGGCTGGAAAATCTGGG
GTTCACCGATGCAATCCGGGCATCGACCGATGAAAGCGGCGTCTATTCTTTCTGGGATTATCAGGCCGGGGCCTGGCAGA
AGAATAACGGCATTCGCATCGACCACCTGATGCTGTCGCCGGAAGCGACAAACCGTTTCATTTCAGCCAATATCGAAAAG
CACGTTCGTGCCTGGGAAAAGCCATCCGATCATGTGCCGGTAACAGTCACGTTGGCGGTTTAG

Upstream 100 bases:

>100_bases
CCGCCGAAATACGAGCTGTGACAGGAATGTGGGAGGAAAAGCTGTGTGGGCCAAAGGGCCGTGCTTGCCCTGCCCCGCAC
GAGCGCATAATCATTATGGA

Downstream 100 bases:

>100_bases
AACGATTCCGTTTTAACCGGAAGCGCCAGAGACATCAGATTGAGACATCGGGCTGTTTTCAGTAACTGCCTTTTACAGCG
TAATCACTTGCAAGCGCGAT

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKIATWNINGVKARIDNLQHWLRESSPDIVCLQEIKSVDEQFPRLEIEALGYHVETHGQKGFNGVALLSKKSPDEINKGL
PGDDSDEQARFIEGIYSTDTGVVRVVSLYLPNGNPIDTEKFPYKLSWMQRLENWAKKRLTLEEPLVLAGDYNVIPEPVDA
RNPQAWLGDALFQPQSRQAFRRLENLGFTDAIRASTDESGVYSFWDYQAGAWQKNNGIRIDHLMLSPEATNRFISANIEK
HVRAWEKPSDHVPVTVTLAV

Sequences:

>Translated_260_residues
MKIATWNINGVKARIDNLQHWLRESSPDIVCLQEIKSVDEQFPRLEIEALGYHVETHGQKGFNGVALLSKKSPDEINKGL
PGDDSDEQARFIEGIYSTDTGVVRVVSLYLPNGNPIDTEKFPYKLSWMQRLENWAKKRLTLEEPLVLAGDYNVIPEPVDA
RNPQAWLGDALFQPQSRQAFRRLENLGFTDAIRASTDESGVYSFWDYQAGAWQKNNGIRIDHLMLSPEATNRFISANIEK
HVRAWEKPSDHVPVTVTLAV
>Mature_260_residues
MKIATWNINGVKARIDNLQHWLRESSPDIVCLQEIKSVDEQFPRLEIEALGYHVETHGQKGFNGVALLSKKSPDEINKGL
PGDDSDEQARFIEGIYSTDTGVVRVVSLYLPNGNPIDTEKFPYKLSWMQRLENWAKKRLTLEEPLVLAGDYNVIPEPVDA
RNPQAWLGDALFQPQSRQAFRRLENLGFTDAIRASTDESGVYSFWDYQAGAWQKNNGIRIDHLMLSPEATNRFISANIEK
HVRAWEKPSDHVPVTVTLAV

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=267, Percent_Identity=32.2097378277154, Blast_Score=131, Evalue=5e-31,
Organism=Homo sapiens, GI18375503, Length=267, Percent_Identity=32.2097378277154, Blast_Score=131, Evalue=5e-31,
Organism=Homo sapiens, GI18375501, Length=267, Percent_Identity=32.2097378277154, Blast_Score=131, Evalue=5e-31,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=36.8421052631579, Blast_Score=174, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI71989536, Length=270, Percent_Identity=30, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI221330655, Length=262, Percent_Identity=32.0610687022901, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI17136678, Length=262, Percent_Identity=32.0610687022901, Blast_Score=123, Evalue=1e-28,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29514; Mature: 29514

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIATWNINGVKARIDNLQHWLRESSPDIVCLQEIKSVDEQFPRLEIEALGYHVETHGQK
CEEEEECCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCEEEEEEEEEEECCCCC
GFNGVALLSKKSPDEINKGLPGDDSDEQARFIEGIYSTDTGVVRVVSLYLPNGNPIDTEK
CCCCEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHEEEEEEECCCCCCCCCCC
FPYKLSWMQRLENWAKKRLTLEEPLVLAGDYNVIPEPVDARNPQAWLGDALFQPQSRQAF
CCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHH
RRLENLGFTDAIRASTDESGVYSFWDYQAGAWQKNNGIRIDHLMLSPEATNRFISANIEK
HHHHHCCCCHHHHCCCCCCCCEEEECCCCCCEECCCCEEEEEEEECCHHHCCEEECCHHH
HVRAWEKPSDHVPVTVTLAV
HHHHHCCCCCCCCEEEEEEC
>Mature Secondary Structure
MKIATWNINGVKARIDNLQHWLRESSPDIVCLQEIKSVDEQFPRLEIEALGYHVETHGQK
CEEEEECCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCEEEEEEEEEEECCCCC
GFNGVALLSKKSPDEINKGLPGDDSDEQARFIEGIYSTDTGVVRVVSLYLPNGNPIDTEK
CCCCEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHEEEEEEECCCCCCCCCCC
FPYKLSWMQRLENWAKKRLTLEEPLVLAGDYNVIPEPVDARNPQAWLGDALFQPQSRQAF
CCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHH
RRLENLGFTDAIRASTDESGVYSFWDYQAGAWQKNNGIRIDHLMLSPEATNRFISANIEK
HHHHHCCCCHHHHCCCCCCCCEEEECCCCCCEECCCCEEEEEEEECCHHHCCEEECCHHH
HVRAWEKPSDHVPVTVTLAV
HHHHHCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]