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The map label for this gene is cobS

Identifier: 23501752

GI number: 23501752

Start: 838845

End: 839627

Strand: Reverse

Name: cobS

Synonym: BR0866

Alternate gene names: 23501752

Gene position: 839627-838845 (Counterclockwise)

Preceding gene: 23501754

Following gene: 23501751

Centisome position: 39.83

GC content: 62.32

Gene sequence:

>783_bases
TTGCAGCGAAATGGCCTGATTGGCGACACGATCAGAAGTCTGGGATTTTTGAGCCGCCTGCCACTGCCGCAAGGCTGGTT
CGACAATACGGACGATTCGCTGCCGCGAAACGCACGCGCCTTTCCGCTTGCGGGCGGCATATTGGGGCTTCTGGCCGGCG
TGGCACTTCTCATTGCCAATGCAATCAGCCTGCCGCCGCTTGCGGCTGCACTGATTGCCATCGGTGCGCTGGCAGCAATG
ACGGGTGCGCTGCATGAAGATGGCCTGGGCGATACGGCCGATGGTTTTTTCGGTGCATCCACGCCAGATCGCCGCCTCGA
TATCATGAAAGATTCGCGCATCGGCACTTTCGCGGCCCTGACGCTGGTTATCTGGACCGGCGTGAAAGCGTCTCTCCTGA
TGGCAATCATCGCGCGCGCGGGCGCAGGCTATGCGCTTCTGGCCCTGATCGGGACGGAAGCAGCCAGCCGCGCAGGAATG
CTGGCCTTCTGGCATGCCCTGCCCTCGGCGCGCCCCGGCGGGCTTGCCGATAGTATGGGCCAGCCGCAATGGGAAACCGT
GGTCTGCGGCTGCGGGCTGGGTCTTGCTCTCCTCGCCATCGGCTTTCTGCCGTCAGGCGGCATGGTGGCCCTCATCAACG
CACTGGTGCTGATGACCGTCGTGCTTTTTGGTTTTGCCAGACTGTGCATGGCAAAAATCGGCGGACAGACCGGCGATACG
CTGGGTGCGGCGCAGCAGATAGGCTCGCTTGCAGCCCTTATCGGACTTGTCATGGCCCTTTGA

Upstream 100 bases:

>100_bases
AAGCACTGACGCGAATTTTCAAATCACCTCTTTGATCTGAAACAATATTGAGGCGAAATATCGCGATGGTTCGACCCTGA
AAACAAAGCGGAGCAGTGAT

Downstream 100 bases:

>100_bases
CGCAACCACATTGCATTTCATGGACACGGCAAAAATCGAATCGCCCTGCATATTGGTATGCACGATGGATGCAAAAACCG
GCTTCTGCCTCGGTTGCGCG

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MQRNGLIGDTIRSLGFLSRLPLPQGWFDNTDDSLPRNARAFPLAGGILGLLAGVALLIANAISLPPLAAALIAIGALAAM
TGALHEDGLGDTADGFFGASTPDRRLDIMKDSRIGTFAALTLVIWTGVKASLLMAIIARAGAGYALLALIGTEAASRAGM
LAFWHALPSARPGGLADSMGQPQWETVVCGCGLGLALLAIGFLPSGGMVALINALVLMTVVLFGFARLCMAKIGGQTGDT
LGAAQQIGSLAALIGLVMAL

Sequences:

>Translated_260_residues
MQRNGLIGDTIRSLGFLSRLPLPQGWFDNTDDSLPRNARAFPLAGGILGLLAGVALLIANAISLPPLAAALIAIGALAAM
TGALHEDGLGDTADGFFGASTPDRRLDIMKDSRIGTFAALTLVIWTGVKASLLMAIIARAGAGYALLALIGTEAASRAGM
LAFWHALPSARPGGLADSMGQPQWETVVCGCGLGLALLAIGFLPSGGMVALINALVLMTVVLFGFARLCMAKIGGQTGDT
LGAAQQIGSLAALIGLVMAL
>Mature_260_residues
MQRNGLIGDTIRSLGFLSRLPLPQGWFDNTDDSLPRNARAFPLAGGILGLLAGVALLIANAISLPPLAAALIAIGALAAM
TGALHEDGLGDTADGFFGASTPDRRLDIMKDSRIGTFAALTLVIWTGVKASLLMAIIARAGAGYALLALIGTEAASRAGM
LAFWHALPSARPGGLADSMGQPQWETVVCGCGLGLALLAIGFLPSGGMVALINALVLMTVVLFGFARLCMAKIGGQTGDT
LGAAQQIGSLAALIGLVMAL

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=246, Percent_Identity=36.5853658536585, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_BRUA1 (B2S5A0)

Other databases:

- EMBL:   CP000887
- RefSeq:   YP_001934821.1
- GeneID:   6327987
- GenomeReviews:   CP000887_GR
- KEGG:   bmc:BAbS19_I08260
- HOGENOM:   HBG729392
- OMA:   WICLLEL
- ProtClustDB:   PRK00235
- HAMAP:   MF_00719
- InterPro:   IPR003805
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 26405; Mature: 26405

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xce9f640)-; HASH(0xb5d053c)-; HASH(0xc7a2e58)-; HASH(0xdb360f8)-; HASH(0xd854040)-; HASH(0xce098bc)-; HASH(0xd7e44dc)-;

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRNGLIGDTIRSLGFLSRLPLPQGWFDNTDDSLPRNARAFPLAGGILGLLAGVALLIAN
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AISLPPLAAALIAIGALAAMTGALHEDGLGDTADGFFGASTPDRRLDIMKDSRIGTFAAL
HHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHH
TLVIWTGVKASLLMAIIARAGAGYALLALIGTEAASRAGMLAFWHALPSARPGGLADSMG
HHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCC
QPQWETVVCGCGLGLALLAIGFLPSGGMVALINALVLMTVVLFGFARLCMAKIGGQTGDT
CCCHHHEEHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
LGAAQQIGSLAALIGLVMAL
HHHHHHHHHHHHHHHHHHHH
>Mature Secondary Structure
MQRNGLIGDTIRSLGFLSRLPLPQGWFDNTDDSLPRNARAFPLAGGILGLLAGVALLIAN
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AISLPPLAAALIAIGALAAMTGALHEDGLGDTADGFFGASTPDRRLDIMKDSRIGTFAAL
HHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHH
TLVIWTGVKASLLMAIIARAGAGYALLALIGTEAASRAGMLAFWHALPSARPGGLADSMG
HHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCC
QPQWETVVCGCGLGLALLAIGFLPSGGMVALINALVLMTVVLFGFARLCMAKIGGQTGDT
CCCHHHEEHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
LGAAQQIGSLAALIGLVMAL
HHHHHHHHHHHHHHHHHHHH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA