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The map label for this gene is yfbT [H]

Identifier: 23501748

GI number: 23501748

Start: 834988

End: 835671

Strand: Reverse

Name: yfbT [H]

Synonym: BR0861

Alternate gene names: 23501748

Gene position: 835671-834988 (Counterclockwise)

Preceding gene: 23501750

Following gene: 23501745

Centisome position: 39.65

GC content: 58.48

Gene sequence:

>684_bases
GTGACCTCCTTTTCCCCTTTCGGCAAATCCTTCGATGCATTTCTTTTCGATATGGACGGCACGATATTGAGTTCCATCGC
AGCGACGGAGCGCGTATGGAGCGAATGGGCAAGGCGCCATGGTATCGATCCCGTGACATTCCTGCCGACCATTCATGGCG
TGCGCGCCGTTGAAACCGTGCGCCGCCTTGCGCTTCCGGGCGTTGACCCAATCCGGGAAGCTGAAATTCTGCTGGAAGCC
GAAATGGCCGATCTGGATGGCATTGCACCGATCGAGGGGGCCTATGAATTTCTGCATTCGCTACCGGAAAACCGCTGGGC
CATCGTGACTTCGGCTCCGCTCGAACTTGCGCGCCGCCGCATCAAGGCCGCCGGGTTGCCCATGCCGAAAACAATCGTTT
CCGCCGAAGACGTGGAACGCGGCAAGCCAAGCCCCGAATGTTTCGAGCTGGGCGCAAAACGGCTGGGCTTTGCGCCGCGT
GATTGCCTTGTTTTCGAGGATGCACCGGCAGGCATCGTGGCAGGAGAAACCGCGGGCGCAAGCGTGGTTGTCGTCACAGC
CACGCATCCGCATTCACCCCGGACCTCGCATTTGAGCATAGAAAGCTATCGCTATCTTCAACTCACCATCGAAGAAAGCG
GAAAACTCAGCCTGAGCCCGACAGTGCCAGCATCAGTTGGCTAA

Upstream 100 bases:

>100_bases
TTTCAACGTGCGACCCGGACTTTCCCACGCTTGCGCATTATGATCGACTGCGGACATATTTTCCGCGATTCTTATCCCCC
GTAGTTCAAGGATATCCGGC

Downstream 100 bases:

>100_bases
AGCACACGCTGGCCTTCCCGCCAGACCGTGCGGATGATCGGCACATGTTCGGCCATCTGCACCCGCACCAGATCGGCGCG
CTTGCCAGCGGCAATCTCGC

Product: HAD superfamily hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MTSFSPFGKSFDAFLFDMDGTILSSIAATERVWSEWARRHGIDPVTFLPTIHGVRAVETVRRLALPGVDPIREAEILLEA
EMADLDGIAPIEGAYEFLHSLPENRWAIVTSAPLELARRRIKAAGLPMPKTIVSAEDVERGKPSPECFELGAKRLGFAPR
DCLVFEDAPAGIVAGETAGASVVVVTATHPHSPRTSHLSIESYRYLQLTIEESGKLSLSPTVPASVG

Sequences:

>Translated_227_residues
MTSFSPFGKSFDAFLFDMDGTILSSIAATERVWSEWARRHGIDPVTFLPTIHGVRAVETVRRLALPGVDPIREAEILLEA
EMADLDGIAPIEGAYEFLHSLPENRWAIVTSAPLELARRRIKAAGLPMPKTIVSAEDVERGKPSPECFELGAKRLGFAPR
DCLVFEDAPAGIVAGETAGASVVVVTATHPHSPRTSHLSIESYRYLQLTIEESGKLSLSPTVPASVG
>Mature_226_residues
TSFSPFGKSFDAFLFDMDGTILSSIAATERVWSEWARRHGIDPVTFLPTIHGVRAVETVRRLALPGVDPIREAEILLEAE
MADLDGIAPIEGAYEFLHSLPENRWAIVTSAPLELARRRIKAAGLPMPKTIVSAEDVERGKPSPECFELGAKRLGFAPRD
CLVFEDAPAGIVAGETAGASVVVVTATHPHSPRTSHLSIESYRYLQLTIEESGKLSLSPTVPASVG

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrate glucose 6-phosphate [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI87082080, Length=204, Percent_Identity=41.6666666666667, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1789046, Length=196, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6321833, Length=205, Percent_Identity=37.0731707317073, Blast_Score=114, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6321834, Length=205, Percent_Identity=35.609756097561, Blast_Score=104, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320905, Length=176, Percent_Identity=30.1136363636364, Blast_Score=71, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322136, Length=197, Percent_Identity=31.9796954314721, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 24560; Mature: 24429

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS01228 COF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSFSPFGKSFDAFLFDMDGTILSSIAATERVWSEWARRHGIDPVTFLPTIHGVRAVETV
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHH
RRLALPGVDPIREAEILLEAEMADLDGIAPIEGAYEFLHSLPENRWAIVTSAPLELARRR
HHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHH
IKAAGLPMPKTIVSAEDVERGKPSPECFELGAKRLGFAPRDCLVFEDAPAGIVAGETAGA
HHHCCCCCCHHHHCHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCEEECCCCCC
SVVVVTATHPHSPRTSHLSIESYRYLQLTIEESGKLSLSPTVPASVG
EEEEEEECCCCCCCCCEEEECCEEEEEEEEECCCCEEECCCCCCCCC
>Mature Secondary Structure 
TSFSPFGKSFDAFLFDMDGTILSSIAATERVWSEWARRHGIDPVTFLPTIHGVRAVETV
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHH
RRLALPGVDPIREAEILLEAEMADLDGIAPIEGAYEFLHSLPENRWAIVTSAPLELARRR
HHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHH
IKAAGLPMPKTIVSAEDVERGKPSPECFELGAKRLGFAPRDCLVFEDAPAGIVAGETAGA
HHHCCCCCCHHHHCHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCEEECCCCCC
SVVVVTATHPHSPRTSHLSIESYRYLQLTIEESGKLSLSPTVPASVG
EEEEEEECCCCCCCCCEEEECCEEEEEEEEECCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 10493123 [H]