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The map label for this gene is bcr [H]

Identifier: 23501720

GI number: 23501720

Start: 811163

End: 812428

Strand: Reverse

Name: bcr [H]

Synonym: BR0833

Alternate gene names: 23501720

Gene position: 812428-811163 (Counterclockwise)

Preceding gene: 23501722

Following gene: 23501715

Centisome position: 38.54

GC content: 58.14

Gene sequence:

>1266_bases
ATGCCGCTCGACATCAAGGACGGTTCGCCAAGCCATCAGACATCCATACGCGGACGCGGTGAATTTATTGCGCTCATCGC
CGCCATCATGGCTATCAATGCCCTCGCTGTCGATATCATGTTGCCGGGCCTGCCGCAGATCGGCGCCAGCCTCGGTGTAC
ATTCTGAAAATCATGTTCAGTTCGTAATCACGGCCTATCTGCTGGGTTTCGGTGTTTCGCAGCTTTTCTATGGCCCATTG
AGCGACCGTTTCGGGCGCAGGCTGCCGCTTTTCGGCAGTCTGGCCATCTATGTTGTCGCCGCACTGGGCTCTGCCTTTGT
AACCGACTTCACGACGCTCATCATTCTGCGTGTGCTGCAAGGGCTTGGCGCTGCCGCAACGCGCGTGATTGCCGTGTCCG
TCGTTCGCGACAAGTTTTCCGGCCGCCAGATGGCGGAAGTCATGTCGCTTGTCATGATGGTTTTCATGATCCTTCCGGTC
GTGGCGCCCGCCACCGGCCAGCTCATCATGCTGTTCGGCGAATGGCACCTGATCTTCATGTTCATGGCCATTATGGCGCT
TGTCGTCGGCCTGTGGGCATTCCTTCGCCTGCCGGAAACGCTCCCCGTGAGCCGTCGCCGCCCGCTCACCATGAAAAGTA
CGCTTGGCGGTTTCGTGATTGTCCTTACGAACCGCGTGGCGCTGTTCTATATGCTGGGGACCTCGTTCATTCTCGGCGCC
CTCTTCGGCTATATCAATTCCGCACAGCAGATTTTTGTCGGCATCTATCAGCTCGGTACATTATTCCCGCTGGCCTTTGC
CGCCGTGGCCATGACGCTTGCGCTTGCCTCGTTCCTGAATTCGCGGCTGGTGGGGCGCTTCGGGATGCGGCGCATTTCCC
AGACCATGCTGCTCGTTTTCACGAGCTTCAGCCTTCTCTGGATGGTGCTGTCTATCGTCATGGATGGCCCCATTCCATTT
GCGGTTCTGATGATAATCTATATGACCATCATGCTCTCGTTCAGCCTTGTGACCGCCAATTTCAATGCGCTGGCCATGGA
ACCCCTGGGCGAAGTGGCTGGCACGGCTTCTTCCGTGCTGGGCTTCGCACAGACCGTCATCGGCGCGGCACTCGGCGCGG
TGATAGGACAGGCTTTCGACGGCACCACCACGCCCGTTGCCACCGGCTATTGCGTGCTCGGTTTCGTGGCGCTTGCCTGT
GTGCTTATCGCCGAACGCGGACGGTTGTTCAGGGTTCAAAATCCGCCAGCCGAACATGTGATCTAA

Upstream 100 bases:

>100_bases
AGCGGCTGCGCGCACAAAGGTCCGGCAAGCCGCTCTGGGATCTTTATTCCGCGCTGCACCTTTCTGAGGCGGCGCACCAA
TATGCCAGGGAATGCCAGCC

Downstream 100 bases:

>100_bases
TCACCCTTCGCAAATGTAAAAGCCCGCCTGACCGGCGGGCTCAGACTGCTGACAAACCTCGGGCAGGGTCAAAATGGCCT
CAGATTCACGGAACGTCACA

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MPLDIKDGSPSHQTSIRGRGEFIALIAAIMAINALAVDIMLPGLPQIGASLGVHSENHVQFVITAYLLGFGVSQLFYGPL
SDRFGRRLPLFGSLAIYVVAALGSAFVTDFTTLIILRVLQGLGAAATRVIAVSVVRDKFSGRQMAEVMSLVMMVFMILPV
VAPATGQLIMLFGEWHLIFMFMAIMALVVGLWAFLRLPETLPVSRRRPLTMKSTLGGFVIVLTNRVALFYMLGTSFILGA
LFGYINSAQQIFVGIYQLGTLFPLAFAAVAMTLALASFLNSRLVGRFGMRRISQTMLLVFTSFSLLWMVLSIVMDGPIPF
AVLMIIYMTIMLSFSLVTANFNALAMEPLGEVAGTASSVLGFAQTVIGAALGAVIGQAFDGTTTPVATGYCVLGFVALAC
VLIAERGRLFRVQNPPAEHVI

Sequences:

>Translated_421_residues
MPLDIKDGSPSHQTSIRGRGEFIALIAAIMAINALAVDIMLPGLPQIGASLGVHSENHVQFVITAYLLGFGVSQLFYGPL
SDRFGRRLPLFGSLAIYVVAALGSAFVTDFTTLIILRVLQGLGAAATRVIAVSVVRDKFSGRQMAEVMSLVMMVFMILPV
VAPATGQLIMLFGEWHLIFMFMAIMALVVGLWAFLRLPETLPVSRRRPLTMKSTLGGFVIVLTNRVALFYMLGTSFILGA
LFGYINSAQQIFVGIYQLGTLFPLAFAAVAMTLALASFLNSRLVGRFGMRRISQTMLLVFTSFSLLWMVLSIVMDGPIPF
AVLMIIYMTIMLSFSLVTANFNALAMEPLGEVAGTASSVLGFAQTVIGAALGAVIGQAFDGTTTPVATGYCVLGFVALAC
VLIAERGRLFRVQNPPAEHVI
>Mature_420_residues
PLDIKDGSPSHQTSIRGRGEFIALIAAIMAINALAVDIMLPGLPQIGASLGVHSENHVQFVITAYLLGFGVSQLFYGPLS
DRFGRRLPLFGSLAIYVVAALGSAFVTDFTTLIILRVLQGLGAAATRVIAVSVVRDKFSGRQMAEVMSLVMMVFMILPVV
APATGQLIMLFGEWHLIFMFMAIMALVVGLWAFLRLPETLPVSRRRPLTMKSTLGGFVIVLTNRVALFYMLGTSFILGAL
FGYINSAQQIFVGIYQLGTLFPLAFAAVAMTLALASFLNSRLVGRFGMRRISQTMLLVFTSFSLLWMVLSIVMDGPIPFA
VLMIIYMTIMLSFSLVTANFNALAMEPLGEVAGTASSVLGFAQTVIGAALGAVIGQAFDGTTTPVATGYCVLGFVALACV
LIAERGRLFRVQNPPAEHVI

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=361, Percent_Identity=30.4709141274238, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1790146, Length=352, Percent_Identity=29.8295454545455, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI87082312, Length=368, Percent_Identity=28.804347826087, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI48994889, Length=232, Percent_Identity=28.0172413793103, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1790794, Length=196, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324264, Length=168, Percent_Identity=30.3571428571429, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45251; Mature: 45120

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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C
>Mature Secondary Structure 
PLDIKDGSPSHQTSIRGRGEFIALIAAIMAINALAVDIMLPGLPQIGASLGVHSENHVQ
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHH
FVITAYLLGFGVSQLFYGPLSDRFGRRLPLFGSLAIYVVAALGSAFVTDFTTLIILRVLQ
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLGAAATRVIAVSVVRDKFSGRQMAEVMSLVMMVFMILPVVAPATGQLIMLFGEWHLIFM
HCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHH
FMAIMALVVGLWAFLRLPETLPVSRRRPLTMKSTLGGFVIVLTNRVALFYMLGTSFILGA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCEEEEHHHHHHHHHHHHHHHHHHH
LFGYINSAQQIFVGIYQLGTLFPLAFAAVAMTLALASFLNSRLVGRFGMRRISQTMLLVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSFSLLWMVLSIVMDGPIPFAVLMIIYMTIMLSFSLVTANFNALAMEPLGEVAGTASSVL
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GFAQTVIGAALGAVIGQAFDGTTTPVATGYCVLGFVALACVLIAERGRLFRVQNPPAEHV
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCC
I
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]