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The map label for this gene is wcaJ [H]

Identifier: 23501668

GI number: 23501668

Start: 764634

End: 766172

Strand: Reverse

Name: wcaJ [H]

Synonym: BR0781

Alternate gene names: 23501668

Gene position: 766172-764634 (Counterclockwise)

Preceding gene: 23501669

Following gene: 23501667

Centisome position: 36.35

GC content: 55.69

Gene sequence:

>1539_bases
GTGCAAGATACACAAGCGCGCTCTCCATCCGGTGGCGAAGCGGCAATTGCTGCCGCCAGCCAGGATTCCGGACCGCAGGA
AAAGACCGGGCTCAGCGATATGGCACGCCAGATGGCGGCGCAATATCAGCGCGAAACGCTTTCTCCTGCCATGCTGAGCG
GAATGATGCGGCTCGTCGAGCTCACACTTGTCTTCGCCACCGGCATTTTAAGCTTTGCTTATTATGTCGGGCTCAACACC
AATCTTAATTTCTATTATCCGCTAATCATCATTGCGGGCAGCGCCTTGTTCGTCATGCTCATGGAGTTGAATGGCGGCTA
TCAGCCTGGCGTCCTGCGCAGCGCCACCCGCAATCTGCGGCGCGTATTCGTCAACTGGGCGACGGTTCTGGGCGCGCTGG
CGATTGCAGGCTTTCTCTTCAAAATTTCATCGCAGTTCTCGCGTGGGTGGTTCATCATCTGGGCCGTAAGCGCGTTCGGC
GTTCTCGTCATCTCCCGCCTGTTTATCGCCTGGAAAATCCGGCGCTGGGCACGAAACGGAACCATGGAACGCCGCGCCAT
CATCGTCGGTGGCGGAACGAATGCGGAATCTCTTATCCGCTCGCTTGAACAGCAGCCGGATAACGACATCCGGATTTGCG
GCATTTTTGATGATCGTGACGACCGCCGCTCACCGCCCATCGTTGCCGGCTATCCGAAAATCGGAAATATCGACGATTTG
ATAGAATTTGCGCGCATTGCGCATATCGACCTGCTCATCATCTCCCTGCCGATCAGTGCCGAATTGCGCGTGCTTTCCCT
GCTTAAAAAACTCTGGGTGCTGCCGGTGGATATCCGGCTTTCAGCACTCAACAATCATCTGCGCTTCCACCCCCGCGCCT
ATTCCTATGTCGGCTCGGTGCCGATGCTGGATATTTTCGACAAGCCAATCAACGACTGGGATTCCGTCGCCAAGCGGATT
TTCGACATTGTTTTCAGCCTGCTCGGAATAATCCTCCTGTCGCCCATCATGCTGGCGACCGCCATTGCCATCAAGCTGGA
CAGCAAAGGCCCGGTAATCTTCAAGCAGAAGCGCCATGGCTTCAATAATGAGGTCATCAATGTCTGGAAGTTCCGCTCCA
TGTATGCGGACAAATGTGATCCGGCCGCAAAGCAAGTCACGACAAAGAACGATCCGCGCGTCACACGCGTCGGCCGTTTC
ATCCGCAAGACCTCCATCGATGAACTGCCCCAGTTTTTCAATGCACTCAGCGGAACGCTCTCGCTGGTCGGCCCCCGCCC
CCATGCCGTGGCGGCCCATTCCCATAATGTGCTGTTCAATGAGGTCGTAGACGGCTATTTCGCGCGCCACCGCGTCAAAC
CCGGCGTCACCGGCTGGGCGCAGATCAATGGCTGGCGCGGTGAAATCGACAATGCGGAAAAAATCCGTATGCGAACCGAA
TATGATCTTTTCTACATCGAAAACTGGTCACTCTGGTTCGACCTCAAGATCCTGTTCCTGACGCCGATCCGGCTTCTGAA
CACGGAAAATGCTTATTGA

Upstream 100 bases:

>100_bases
TTCCAGAAGCTCCTGGACGCATGGTTTTCTTCCGCGCAGAGATTTCTTAGGGAACTGATTTTAGGTATAGTCCTGCAATT
CGAGCAGCAGGGGCAAAATC

Downstream 100 bases:

>100_bases
ACATTATCGCCCTGTTAGAGCCGTTTCGATCATAATCTTGTTTCCCCCCATTCCACTCCGGTCGGATTATGCTCGAACCT
CCATGCCCCTGATTGTCGCG

Product: sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MQDTQARSPSGGEAAIAAASQDSGPQEKTGLSDMARQMAAQYQRETLSPAMLSGMMRLVELTLVFATGILSFAYYVGLNT
NLNFYYPLIIIAGSALFVMLMELNGGYQPGVLRSATRNLRRVFVNWATVLGALAIAGFLFKISSQFSRGWFIIWAVSAFG
VLVISRLFIAWKIRRWARNGTMERRAIIVGGGTNAESLIRSLEQQPDNDIRICGIFDDRDDRRSPPIVAGYPKIGNIDDL
IEFARIAHIDLLIISLPISAELRVLSLLKKLWVLPVDIRLSALNNHLRFHPRAYSYVGSVPMLDIFDKPINDWDSVAKRI
FDIVFSLLGIILLSPIMLATAIAIKLDSKGPVIFKQKRHGFNNEVINVWKFRSMYADKCDPAAKQVTTKNDPRVTRVGRF
IRKTSIDELPQFFNALSGTLSLVGPRPHAVAAHSHNVLFNEVVDGYFARHRVKPGVTGWAQINGWRGEIDNAEKIRMRTE
YDLFYIENWSLWFDLKILFLTPIRLLNTENAY

Sequences:

>Translated_512_residues
MQDTQARSPSGGEAAIAAASQDSGPQEKTGLSDMARQMAAQYQRETLSPAMLSGMMRLVELTLVFATGILSFAYYVGLNT
NLNFYYPLIIIAGSALFVMLMELNGGYQPGVLRSATRNLRRVFVNWATVLGALAIAGFLFKISSQFSRGWFIIWAVSAFG
VLVISRLFIAWKIRRWARNGTMERRAIIVGGGTNAESLIRSLEQQPDNDIRICGIFDDRDDRRSPPIVAGYPKIGNIDDL
IEFARIAHIDLLIISLPISAELRVLSLLKKLWVLPVDIRLSALNNHLRFHPRAYSYVGSVPMLDIFDKPINDWDSVAKRI
FDIVFSLLGIILLSPIMLATAIAIKLDSKGPVIFKQKRHGFNNEVINVWKFRSMYADKCDPAAKQVTTKNDPRVTRVGRF
IRKTSIDELPQFFNALSGTLSLVGPRPHAVAAHSHNVLFNEVVDGYFARHRVKPGVTGWAQINGWRGEIDNAEKIRMRTE
YDLFYIENWSLWFDLKILFLTPIRLLNTENAY
>Mature_512_residues
MQDTQARSPSGGEAAIAAASQDSGPQEKTGLSDMARQMAAQYQRETLSPAMLSGMMRLVELTLVFATGILSFAYYVGLNT
NLNFYYPLIIIAGSALFVMLMELNGGYQPGVLRSATRNLRRVFVNWATVLGALAIAGFLFKISSQFSRGWFIIWAVSAFG
VLVISRLFIAWKIRRWARNGTMERRAIIVGGGTNAESLIRSLEQQPDNDIRICGIFDDRDDRRSPPIVAGYPKIGNIDDL
IEFARIAHIDLLIISLPISAELRVLSLLKKLWVLPVDIRLSALNNHLRFHPRAYSYVGSVPMLDIFDKPINDWDSVAKRI
FDIVFSLLGIILLSPIMLATAIAIKLDSKGPVIFKQKRHGFNNEVINVWKFRSMYADKCDPAAKQVTTKNDPRVTRVGRF
IRKTSIDELPQFFNALSGTLSLVGPRPHAVAAHSHNVLFNEVVDGYFARHRVKPGVTGWAQINGWRGEIDNAEKIRMRTE
YDLFYIENWSLWFDLKILFLTPIRLLNTENAY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=483, Percent_Identity=34.9896480331263, Blast_Score=269, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 57655; Mature: 57655

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDTQARSPSGGEAAIAAASQDSGPQEKTGLSDMARQMAAQYQRETLSPAMLSGMMRLVE
CCCCCCCCCCCCCEEEEECCCCCCCCHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LTLVFATGILSFAYYVGLNTNLNFYYPLIIIAGSALFVMLMELNGGYQPGVLRSATRNLR
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
RVFVNWATVLGALAIAGFLFKISSQFSRGWFIIWAVSAFGVLVISRLFIAWKIRRWARNG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCC
TMERRAIIVGGGTNAESLIRSLEQQPDNDIRICGIFDDRDDRRSPPIVAGYPKIGNIDDL
CCCEEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCEEECCCCCCCHHHH
IEFARIAHIDLLIISLPISAELRVLSLLKKLWVLPVDIRLSALNNHLRFHPRAYSYVGSV
HHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHCCCEEEEHHHHCCCCEECCHHHHHHCCC
PMLDIFDKPINDWDSVAKRIFDIVFSLLGIILLSPIMLATAIAIKLDSKGPVIFKQKRHG
CCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEECCCCC
FNNEVINVWKFRSMYADKCDPAAKQVTTKNDPRVTRVGRFIRKTSIDELPQFFNALSGTL
CCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCE
SLVGPRPHAVAAHSHNVLFNEVVDGYFARHRVKPGVTGWAQINGWRGEIDNAEKIRMRTE
EEECCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCHHEEEEEE
YDLFYIENWSLWFDLKILFLTPIRLLNTENAY
EEEEEEECCEEEEEEEEHHHCCHHHCCCCCCC
>Mature Secondary Structure
MQDTQARSPSGGEAAIAAASQDSGPQEKTGLSDMARQMAAQYQRETLSPAMLSGMMRLVE
CCCCCCCCCCCCCEEEEECCCCCCCCHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LTLVFATGILSFAYYVGLNTNLNFYYPLIIIAGSALFVMLMELNGGYQPGVLRSATRNLR
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
RVFVNWATVLGALAIAGFLFKISSQFSRGWFIIWAVSAFGVLVISRLFIAWKIRRWARNG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCC
TMERRAIIVGGGTNAESLIRSLEQQPDNDIRICGIFDDRDDRRSPPIVAGYPKIGNIDDL
CCCEEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCEEECCCCCCCHHHH
IEFARIAHIDLLIISLPISAELRVLSLLKKLWVLPVDIRLSALNNHLRFHPRAYSYVGSV
HHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHCCCEEEEHHHHCCCCEECCHHHHHHCCC
PMLDIFDKPINDWDSVAKRIFDIVFSLLGIILLSPIMLATAIAIKLDSKGPVIFKQKRHG
CCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEECCCCC
FNNEVINVWKFRSMYADKCDPAAKQVTTKNDPRVTRVGRFIRKTSIDELPQFFNALSGTL
CCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCE
SLVGPRPHAVAAHSHNVLFNEVVDGYFARHRVKPGVTGWAQINGWRGEIDNAEKIRMRTE
EEECCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCHHEEEEEE
YDLFYIENWSLWFDLKILFLTPIRLLNTENAY
EEEEEEECCEEEEEEEEHHHCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]