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The map label for this gene is lptD [H]

Identifier: 23501572

GI number: 23501572

Start: 671639

End: 674017

Strand: Reverse

Name: lptD [H]

Synonym: BR0685

Alternate gene names: 23501572

Gene position: 674017-671639 (Counterclockwise)

Preceding gene: 23501573

Following gene: 23501571

Centisome position: 31.98

GC content: 55.57

Gene sequence:

>2379_bases
ATGGTATTGCCCCATACGCTCTCGCGCCTTGCGCGCGGGACTGCATTGGCGTGCGTCCTTGCTTTGCCTTTTGTTTCTGT
CGCAATTCTCTCCTCGCCTGCCCAGGCGCAGGATGCCCTGTCAGCCAATTACCAGAGCGATCCAAACGCCCGCATGCTGC
TTCAGGCAGATGAACTCGTCTATGATCGCGATGTGAACACCGTTACCGCGCAAGGTAAGGTTCGCATTGAATATGACGGC
AACCGGCTGGTCGCCGACAAGGTTACCTATAACCAGCAAACCCGCCGCATGACCGCGACCGGCAATGTCGAAATCGTGGA
GCGCGACGGCAACCGGATCTATTCCGACCATATCGATGTGACCGACAGTTTCCGCGATGGCTTTGTGAACGGCCTGCGCG
TCGAGACGACGGATAATACGCGGTTTGTCGCTGAGAGCGCCGAACGCAGCAACGGTGAAATCACCACATTCAACAATGGC
GCCTATACCGCCTGCGAACCCTGCGCCAAGAATCCAGACAAGCCCGTTCTGTGGCAGATCAAGGCGCGCAAGATCATCTG
GAACAGTGCAACGAAAACCGTTCGTTTCGAGCGGGGCCGTTTTGAGCTGTTTGGCATGCCGCTTGCCTATCTGCCTGCTT
TCGAGATGGCCGACCCGACGGTCAAACGCAAGAGCGGCTTTCTTTTCCCGGGTTTCGCCTATAAGGACGATCTGGGTTTC
GGCATCAAGAATTCCTATTTCTGGGCGCTTGCGCCCAATTATGACCTGACGCTTTCCACGACAGCCTATACCAAACAAGG
TTTCCTTACCGAAGCCGAATGGCGCCATCGTCTGGAAAACGGCGAGTATGATTTCCGCATTGCCGGTATTCATCAGTTGA
AGCCGGAGGAATTCGGCGTCACCACGATCGACCGCGAGAAAACGAATCGCGGTATGGTGGCCTCCAAGGGTAATTTCGAT
ATTAATTCGCGCTGGCATTTCGGTTGGGATGTGCTGGCCCAGACCGACCATAATTTCAGCCGCACCTATGAAATCCAGGG
CTACAATGCCCAGACGCAGGTTTCAAAAATCTACCTGACGGGCATCAACAACCGTAACTATTTCGATCTGAATTTCTATC
GCTTCAACGTTCAGGAATCCTACCTGGCGGGCGATCCGAATGAAATGTATTCAAAGCAGCCCTGGGTATTCCCAAGCCTG
GATTATTCCTACACCATGCCGGAGCCGGTCTATGGTGGTGAGTTGAATTTCACGACCAATTTGCAGGCGCTTTATCGAAA
GAATGCGGATTATACGAATCCTTTCATCAGCGTTGACGAGAATGGTTCCTGGGTCACAAAGCCCAATCCCTACCCTCGCA
TTCCGGGCTTCAGCGGCACCAATCTACGCTTCACCAGTGAAGCCGAATGGAAGCGGACCTTCATCACGCCTTCGGGTCTT
GTGATTACGCCGCTTATGGCGCTGCGGGGCGATGCCATTCGCGTAGACACGAATTTTGATCCGGCAAATGCGGGTTTCAC
CGATGCTGTCGTTCGGTCAGAAGCACTGCGCGCCATGGCGACGGCCGGGCTTGAATTGCGCTGGCCGATCCTGTTCTCAA
CCACCAGTTCCACACATATTCTGGAGCCGGTTGCACAGATCTTTGTCCGCAACAATGAACGCTATGCCGGCCAGCTTCCC
AATGAAGATGCGCAGAGCTTCGTTTTCGACGCCTCCAATCTCTTCTCGCGTGACAAATTCTCCGGCTATGACCGTGTGGA
AGGCGGTACGCGCGCCAATCTCGGCCTGCGCTATTCTGGTAATTTCAAGGATAGCGACTGGGCACTTTATGCTCTTGGCG
GCCAGTCCTTCCAGCTTGGCGGCCTGAACTCCTACGCCGCCAGCGATTTCGTCAATGTCGGTGCGGATTCCGGCCTGGAG
GATGCGCGCTCCGATTATGTCGCCATGATCGGCACGTCGAACTCGACCGGTCTGGCGCTTGCCGCCCGCGGACGCTTCGG
CAAGGATGATTTCGCCGTCCAGCGCGGCGAATTCGAGGCGCAGCAAAGCTGGGAGAAGCTAACCGTTTCGGGCCAATATG
CCTATATTGCTCCGCAGCCAGCCTATGGCTATTCCGATCTCCGACAGGAAGTTACAGGGTCTGCCACGGCGCGGATCAAT
ACCAACTGGCGGGTTTTCGGCTCCGGCACCTATGATCTCGTATCGGATACGCTGGTGCGGGCCTCATCTGGCCTTGCCTA
TGACGATGAGTGCTTCACCTATTCGATGGCCTATATCCAGACCCGCAATCCGGGTGACGAAAAGGCATCGCATAGCGTTG
GCTTCACGATTTCGCTGCGCACGCTCGGCGATTTCGGCAACGGTTCGCAGACTTTCTAG

Upstream 100 bases:

>100_bases
CATTCGTGGCCGCTTGGGTTCCTGTAGTTATTGCGACATTCTTTGGTGTCTCCTTTTTGTTGCATAAGGAGGATGGTTAG
TGGGTTTGAGTAACACCCGC

Downstream 100 bases:

>100_bases
GGAAACGGGCTGGAAGTACGGTCAATGCATCCGGCATTTTCTTTTGTTTGCGCAACAATCTCCAGCCTGCCATTGGAAAC
CTGTTTTCAGTTGGAGCGGC

Product: organic solvent tolerance

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 792; Mature: 792

Protein sequence:

>792_residues
MVLPHTLSRLARGTALACVLALPFVSVAILSSPAQAQDALSANYQSDPNARMLLQADELVYDRDVNTVTAQGKVRIEYDG
NRLVADKVTYNQQTRRMTATGNVEIVERDGNRIYSDHIDVTDSFRDGFVNGLRVETTDNTRFVAESAERSNGEITTFNNG
AYTACEPCAKNPDKPVLWQIKARKIIWNSATKTVRFERGRFELFGMPLAYLPAFEMADPTVKRKSGFLFPGFAYKDDLGF
GIKNSYFWALAPNYDLTLSTTAYTKQGFLTEAEWRHRLENGEYDFRIAGIHQLKPEEFGVTTIDREKTNRGMVASKGNFD
INSRWHFGWDVLAQTDHNFSRTYEIQGYNAQTQVSKIYLTGINNRNYFDLNFYRFNVQESYLAGDPNEMYSKQPWVFPSL
DYSYTMPEPVYGGELNFTTNLQALYRKNADYTNPFISVDENGSWVTKPNPYPRIPGFSGTNLRFTSEAEWKRTFITPSGL
VITPLMALRGDAIRVDTNFDPANAGFTDAVVRSEALRAMATAGLELRWPILFSTTSSTHILEPVAQIFVRNNERYAGQLP
NEDAQSFVFDASNLFSRDKFSGYDRVEGGTRANLGLRYSGNFKDSDWALYALGGQSFQLGGLNSYAASDFVNVGADSGLE
DARSDYVAMIGTSNSTGLALAARGRFGKDDFAVQRGEFEAQQSWEKLTVSGQYAYIAPQPAYGYSDLRQEVTGSATARIN
TNWRVFGSGTYDLVSDTLVRASSGLAYDDECFTYSMAYIQTRNPGDEKASHSVGFTISLRTLGDFGNGSQTF

Sequences:

>Translated_792_residues
MVLPHTLSRLARGTALACVLALPFVSVAILSSPAQAQDALSANYQSDPNARMLLQADELVYDRDVNTVTAQGKVRIEYDG
NRLVADKVTYNQQTRRMTATGNVEIVERDGNRIYSDHIDVTDSFRDGFVNGLRVETTDNTRFVAESAERSNGEITTFNNG
AYTACEPCAKNPDKPVLWQIKARKIIWNSATKTVRFERGRFELFGMPLAYLPAFEMADPTVKRKSGFLFPGFAYKDDLGF
GIKNSYFWALAPNYDLTLSTTAYTKQGFLTEAEWRHRLENGEYDFRIAGIHQLKPEEFGVTTIDREKTNRGMVASKGNFD
INSRWHFGWDVLAQTDHNFSRTYEIQGYNAQTQVSKIYLTGINNRNYFDLNFYRFNVQESYLAGDPNEMYSKQPWVFPSL
DYSYTMPEPVYGGELNFTTNLQALYRKNADYTNPFISVDENGSWVTKPNPYPRIPGFSGTNLRFTSEAEWKRTFITPSGL
VITPLMALRGDAIRVDTNFDPANAGFTDAVVRSEALRAMATAGLELRWPILFSTTSSTHILEPVAQIFVRNNERYAGQLP
NEDAQSFVFDASNLFSRDKFSGYDRVEGGTRANLGLRYSGNFKDSDWALYALGGQSFQLGGLNSYAASDFVNVGADSGLE
DARSDYVAMIGTSNSTGLALAARGRFGKDDFAVQRGEFEAQQSWEKLTVSGQYAYIAPQPAYGYSDLRQEVTGSATARIN
TNWRVFGSGTYDLVSDTLVRASSGLAYDDECFTYSMAYIQTRNPGDEKASHSVGFTISLRTLGDFGNGSQTF
>Mature_792_residues
MVLPHTLSRLARGTALACVLALPFVSVAILSSPAQAQDALSANYQSDPNARMLLQADELVYDRDVNTVTAQGKVRIEYDG
NRLVADKVTYNQQTRRMTATGNVEIVERDGNRIYSDHIDVTDSFRDGFVNGLRVETTDNTRFVAESAERSNGEITTFNNG
AYTACEPCAKNPDKPVLWQIKARKIIWNSATKTVRFERGRFELFGMPLAYLPAFEMADPTVKRKSGFLFPGFAYKDDLGF
GIKNSYFWALAPNYDLTLSTTAYTKQGFLTEAEWRHRLENGEYDFRIAGIHQLKPEEFGVTTIDREKTNRGMVASKGNFD
INSRWHFGWDVLAQTDHNFSRTYEIQGYNAQTQVSKIYLTGINNRNYFDLNFYRFNVQESYLAGDPNEMYSKQPWVFPSL
DYSYTMPEPVYGGELNFTTNLQALYRKNADYTNPFISVDENGSWVTKPNPYPRIPGFSGTNLRFTSEAEWKRTFITPSGL
VITPLMALRGDAIRVDTNFDPANAGFTDAVVRSEALRAMATAGLELRWPILFSTTSSTHILEPVAQIFVRNNERYAGQLP
NEDAQSFVFDASNLFSRDKFSGYDRVEGGTRANLGLRYSGNFKDSDWALYALGGQSFQLGGLNSYAASDFVNVGADSGLE
DARSDYVAMIGTSNSTGLALAARGRFGKDDFAVQRGEFEAQQSWEKLTVSGQYAYIAPQPAYGYSDLRQEVTGSATARIN
TNWRVFGSGTYDLVSDTLVRASSGLAYDDECFTYSMAYIQTRNPGDEKASHSVGFTISLRTLGDFGNGSQTF

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=184, Percent_Identity=23.9130434782609, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 88554; Mature: 88554

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLPHTLSRLARGTALACVLALPFVSVAILSSPAQAQDALSANYQSDPNARMLLQADELV
CCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEEECCEE
YDRDVNTVTAQGKVRIEYDGNRLVADKVTYNQQTRRMTATGNVEIVERDGNRIYSDHIDV
EECCCCEEEECCEEEEEECCCEEEEEEECCCCCCEEEEECCCEEEEECCCCEEEECCCCC
TDSFRDGFVNGLRVETTDNTRFVAESAERSNGEITTFNNGAYTACEPCAKNPDKPVLWQI
CCCHHHHCCCCEEEEECCCCEEEEECCCCCCCCEEEECCCCCEECCHHHCCCCCCEEEEE
KARKIIWNSATKTVRFERGRFELFGMPLAYLPAFEMADPTVKRKSGFLFPGFAYKDDLGF
EEEEEEECCCCCEEEEECCCEEEECCCHHHCCCHHCCCCCHHHCCCEECCCCCCCCCCCC
GIKNSYFWALAPNYDLTLSTTAYTKQGFLTEAEWRHRLENGEYDFRIAGIHQLKPEEFGV
CCCCCEEEEECCCCCEEEEEEEEECCCCCCCHHHHHHHCCCCEEEEEECEEECCCHHCCC
TTIDREKTNRGMVASKGNFDINSRWHFGWDVLAQTDHNFSRTYEIQGYNAQTQVSKIYLT
EEECCCCCCCCEEEECCCCCCCCEEECCEEEEEECCCCCCEEEEEECCCCCCEEEEEEEE
GINNRNYFDLNFYRFNVQESYLAGDPNEMYSKQPWVFPSLDYSYTMPEPVYGGELNFTTN
ECCCCCEEEEEEEEEECCHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECC
LQALYRKNADYTNPFISVDENGSWVTKPNPYPRIPGFSGTNLRFTSEAEWKRTFITPSGL
HHHHHHCCCCCCCCEEEECCCCCEEECCCCCCCCCCCCCCCEEEECCCCCEEEEECCCCH
VITPLMALRGDAIRVDTNFDPANAGFTDAVVRSEALRAMATAGLELRWPILFSTTSSTHI
HHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHH
LEPVAQIFVRNNERYAGQLPNEDAQSFVFDASNLFSRDKFSGYDRVEGGTRANLGLRYSG
HHHHHHHHEECCCCEECCCCCCCHHHEEEECHHHHCCCCCCCCCCCCCCCEECEEEEECC
NFKDSDWALYALGGQSFQLGGLNSYAASDFVNVGADSGLEDARSDYVAMIGTSNSTGLAL
CCCCCCEEEEEECCCEEEECCCCCHHHHHHHCCCCCCCCCHHCCCEEEEEECCCCCCEEE
AARGRFGKDDFAVQRGEFEAQQSWEKLTVSGQYAYIAPQPAYGYSDLRQEVTGSATARIN
EECCCCCCCHHEEECCCCHHHHCHHEEEECCEEEEECCCCCCCHHHHHHHHCCCEEEEEE
TNWRVFGSGTYDLVSDTLVRASSGLAYDDECFTYSMAYIQTRNPGDEKASHSVGFTISLR
CCEEEEECCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCEEEEEEE
TLGDFGNGSQTF
EECCCCCCCCCC
>Mature Secondary Structure
MVLPHTLSRLARGTALACVLALPFVSVAILSSPAQAQDALSANYQSDPNARMLLQADELV
CCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEEECCEE
YDRDVNTVTAQGKVRIEYDGNRLVADKVTYNQQTRRMTATGNVEIVERDGNRIYSDHIDV
EECCCCEEEECCEEEEEECCCEEEEEEECCCCCCEEEEECCCEEEEECCCCEEEECCCCC
TDSFRDGFVNGLRVETTDNTRFVAESAERSNGEITTFNNGAYTACEPCAKNPDKPVLWQI
CCCHHHHCCCCEEEEECCCCEEEEECCCCCCCCEEEECCCCCEECCHHHCCCCCCEEEEE
KARKIIWNSATKTVRFERGRFELFGMPLAYLPAFEMADPTVKRKSGFLFPGFAYKDDLGF
EEEEEEECCCCCEEEEECCCEEEECCCHHHCCCHHCCCCCHHHCCCEECCCCCCCCCCCC
GIKNSYFWALAPNYDLTLSTTAYTKQGFLTEAEWRHRLENGEYDFRIAGIHQLKPEEFGV
CCCCCEEEEECCCCCEEEEEEEEECCCCCCCHHHHHHHCCCCEEEEEECEEECCCHHCCC
TTIDREKTNRGMVASKGNFDINSRWHFGWDVLAQTDHNFSRTYEIQGYNAQTQVSKIYLT
EEECCCCCCCCEEEECCCCCCCCEEECCEEEEEECCCCCCEEEEEECCCCCCEEEEEEEE
GINNRNYFDLNFYRFNVQESYLAGDPNEMYSKQPWVFPSLDYSYTMPEPVYGGELNFTTN
ECCCCCEEEEEEEEEECCHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECC
LQALYRKNADYTNPFISVDENGSWVTKPNPYPRIPGFSGTNLRFTSEAEWKRTFITPSGL
HHHHHHCCCCCCCCEEEECCCCCEEECCCCCCCCCCCCCCCEEEECCCCCEEEEECCCCH
VITPLMALRGDAIRVDTNFDPANAGFTDAVVRSEALRAMATAGLELRWPILFSTTSSTHI
HHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHH
LEPVAQIFVRNNERYAGQLPNEDAQSFVFDASNLFSRDKFSGYDRVEGGTRANLGLRYSG
HHHHHHHHEECCCCEECCCCCCCHHHEEEECHHHHCCCCCCCCCCCCCCCEECEEEEECC
NFKDSDWALYALGGQSFQLGGLNSYAASDFVNVGADSGLEDARSDYVAMIGTSNSTGLAL
CCCCCCEEEEEECCCEEEECCCCCHHHHHHHCCCCCCCCCHHCCCEEEEEECCCCCCEEE
AARGRFGKDDFAVQRGEFEAQQSWEKLTVSGQYAYIAPQPAYGYSDLRQEVTGSATARIN
EECCCCCCCHHEEECCCCHHHHCHHEEEECCEEEEECCCCCCCHHHHHHHHCCCEEEEEE
TNWRVFGSGTYDLVSDTLVRASSGLAYDDECFTYSMAYIQTRNPGDEKASHSVGFTISLR
CCEEEEECCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCEEEEEEE
TLGDFGNGSQTF
EECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA