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The map label for this gene is bsp6IM [H]

Identifier: 23100788

GI number: 23100788

Start: 3469736

End: 3470848

Strand: Reverse

Name: bsp6IM [H]

Synonym: OB3333

Alternate gene names: 23100788

Gene position: 3470848-3469736 (Counterclockwise)

Preceding gene: 23100789

Following gene: 23100784

Centisome position: 95.6

GC content: 33.6

Gene sequence:

>1113_bases
ATGACGTATTCAGTTGCAAGCTTGTTTGCTGGTATTGGCGGAATTGATATAGGATTTGAACAAGCTGGTGCAAGAGTTAT
CTGGGCTAATGAAATGGATAAGCACGCTTGTGAAACGTATCGCCAAAATTTCGAGAGTAAACTAATTGAAGATGATGTAA
GAAATATAAAAGAAAGTGACATGCCTGATGCTGATATAATAACTGCTGGATGGCCTTGTGTTGCATTTAGCATTGCAGGT
AACAGACATGGTATGAAATATAAATGTAACAATTGTGAGCATGAACACAGTGTAACTTACGATGAGTATATACATGGAGT
TACATGTCCAAAATGTGCTGGGCATACAGAAGCAATAGATCCAAGAGGGACACTTTTTTTTGATGTTATCCGCTTTATTC
GCGCTAAGAATCCTGAAGCAATTTTTTTGGAAAATGTAAAAAATTTAAGAGGTCATGACAAAGGGAATACTTTTAAAGTA
ATTACTGAAATGCTTCAAGAAAGTGGTTATTATATAGATAGCAAAATAATGAATACAATGGATTATGGAAACATACCTCA
GAACAGAGAGCGAATATTTATTGTAGGATTTAAAGATGAAGAGAAATATAATAAATTTCAATGGCCCCAAAAAATTGGAC
TTACAAAATCAATTGATAATGTAATAGATCGCAGCAAGAAGCAGGATGATGCTTATTATTATACGGAAGAAAGTCAATAC
TATTCAATGATCGTTGAGTCTATGAAAAATAGAAATACTGTATATCAGCTTAGAAGAGTCTATATGAGAGAAAATCAAAG
CAATGTTTGTCCTACACTAACTGCAAATATGGGTACTGGAGGACATAATGTACCTCTTATAATGGATGACTGGGGATATA
GAAAAATCACACCTAAAGAGGCACTGAAGTTTCAAGGTTTCCTTGTCGGTGAAAATTATAAAATTCCTGAAGATATGGCA
AAAAGTCATTTATATAAACAGGCAGGAAATGCTGTAAGTGTTCCAGTAATCAGAAGAATTGCAACTAATTTATTGGAAGC
ATTAGGATTTGGAGAAACAGAATATAAAACGATTGAGAAAAATGAAAAACATGAGCAGCTTTCATTTAACTAA

Upstream 100 bases:

>100_bases
TATAATAGTTTCCTATTAAATATTTAATTTAAGAGTGGTTTTATTATTTAGAGTAGCTAATTTCAATGTATTAGTTAAAT
ATTTTGGGAGGCTTGTCAAA

Downstream 100 bases:

>100_bases
AGCTAGTTTACTTGTATTAAAAAATCACCTTACACTTGATAAGGTGATTTTTTTAACAAAATATTAAATTTGTCCCAAAT
GAAAATAATATCTAGTTCTC

Product: cytosine-specific DNA-methyltransferase

Products: NA

Alternate protein names: M.Bsp6I; Cytosine-specific methyltransferase Bsp6I [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MTYSVASLFAGIGGIDIGFEQAGARVIWANEMDKHACETYRQNFESKLIEDDVRNIKESDMPDADIITAGWPCVAFSIAG
NRHGMKYKCNNCEHEHSVTYDEYIHGVTCPKCAGHTEAIDPRGTLFFDVIRFIRAKNPEAIFLENVKNLRGHDKGNTFKV
ITEMLQESGYYIDSKIMNTMDYGNIPQNRERIFIVGFKDEEKYNKFQWPQKIGLTKSIDNVIDRSKKQDDAYYYTEESQY
YSMIVESMKNRNTVYQLRRVYMRENQSNVCPTLTANMGTGGHNVPLIMDDWGYRKITPKEALKFQGFLVGENYKIPEDMA
KSHLYKQAGNAVSVPVIRRIATNLLEALGFGETEYKTIEKNEKHEQLSFN

Sequences:

>Translated_370_residues
MTYSVASLFAGIGGIDIGFEQAGARVIWANEMDKHACETYRQNFESKLIEDDVRNIKESDMPDADIITAGWPCVAFSIAG
NRHGMKYKCNNCEHEHSVTYDEYIHGVTCPKCAGHTEAIDPRGTLFFDVIRFIRAKNPEAIFLENVKNLRGHDKGNTFKV
ITEMLQESGYYIDSKIMNTMDYGNIPQNRERIFIVGFKDEEKYNKFQWPQKIGLTKSIDNVIDRSKKQDDAYYYTEESQY
YSMIVESMKNRNTVYQLRRVYMRENQSNVCPTLTANMGTGGHNVPLIMDDWGYRKITPKEALKFQGFLVGENYKIPEDMA
KSHLYKQAGNAVSVPVIRRIATNLLEALGFGETEYKTIEKNEKHEQLSFN
>Mature_369_residues
TYSVASLFAGIGGIDIGFEQAGARVIWANEMDKHACETYRQNFESKLIEDDVRNIKESDMPDADIITAGWPCVAFSIAGN
RHGMKYKCNNCEHEHSVTYDEYIHGVTCPKCAGHTEAIDPRGTLFFDVIRFIRAKNPEAIFLENVKNLRGHDKGNTFKVI
TEMLQESGYYIDSKIMNTMDYGNIPQNRERIFIVGFKDEEKYNKFQWPQKIGLTKSIDNVIDRSKKQDDAYYYTEESQYY
SMIVESMKNRNTVYQLRRVYMRENQSNVCPTLTANMGTGGHNVPLIMDDWGYRKITPKEALKFQGFLVGENYKIPEDMAK
SHLYKQAGNAVSVPVIRRIATNLLEALGFGETEYKTIEKNEKHEQLSFN

Specific function: This methylase recognizes the double-stranded sequence GCNGC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the Bsp6I endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=387, Percent_Identity=29.1989664082687, Blast_Score=131, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 42520; Mature: 42388

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYSVASLFAGIGGIDIGFEQAGARVIWANEMDKHACETYRQNFESKLIEDDVRNIKESD
CCCCHHHHHHCCCCCCCCHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MPDADIITAGWPCVAFSIAGNRHGMKYKCNNCEHEHSVTYDEYIHGVTCPKCAGHTEAID
CCCCCEEECCCCEEEEEECCCCCCCCEECCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCC
PRGTLFFDVIRFIRAKNPEAIFLENVKNLRGHDKGNTFKVITEMLQESGYYIDSKIMNTM
CCCHHHHHHHHHHHCCCCCEEEHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEHHHHHHHH
DYGNIPQNRERIFIVGFKDEEKYNKFQWPQKIGLTKSIDNVIDRSKKQDDAYYYTEESQY
CCCCCCCCCCEEEEEEECCCHHHHHHCCHHHCCCHHHHHHHHHHCCCCCCCEEEECCHHH
YSMIVESMKNRNTVYQLRRVYMRENQSNVCPTLTANMGTGGHNVPLIMDDWGYRKITPKE
HHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCEEECCCCCCCCCHHH
ALKFQGFLVGENYKIPEDMAKSHLYKQAGNAVSVPVIRRIATNLLEALGFGETEYKTIEK
HHHCCCEEECCCCCCCHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHCCCCHHHHHHHC
NEKHEQLSFN
CCCCCCCCCC
>Mature Secondary Structure 
TYSVASLFAGIGGIDIGFEQAGARVIWANEMDKHACETYRQNFESKLIEDDVRNIKESD
CCCHHHHHHCCCCCCCCHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MPDADIITAGWPCVAFSIAGNRHGMKYKCNNCEHEHSVTYDEYIHGVTCPKCAGHTEAID
CCCCCEEECCCCEEEEEECCCCCCCCEECCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCC
PRGTLFFDVIRFIRAKNPEAIFLENVKNLRGHDKGNTFKVITEMLQESGYYIDSKIMNTM
CCCHHHHHHHHHHHCCCCCEEEHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEHHHHHHHH
DYGNIPQNRERIFIVGFKDEEKYNKFQWPQKIGLTKSIDNVIDRSKKQDDAYYYTEESQY
CCCCCCCCCCEEEEEEECCCHHHHHHCCHHHCCCHHHHHHHHHHCCCCCCCEEEECCHHH
YSMIVESMKNRNTVYQLRRVYMRENQSNVCPTLTANMGTGGHNVPLIMDDWGYRKITPKE
HHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCEEECCCCCCCCCHHH
ALKFQGFLVGENYKIPEDMAKSHLYKQAGNAVSVPVIRRIATNLLEALGFGETEYKTIEK
HHHCCCEEECCCCCCCHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHCCCCHHHHHHHC
NEKHEQLSFN
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7607501 [H]