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The map label for this gene is iaaH [H]

Identifier: 23100738

GI number: 23100738

Start: 3420304

End: 3421602

Strand: Reverse

Name: iaaH [H]

Synonym: OB3283

Alternate gene names: 23100738

Gene position: 3421602-3420304 (Counterclockwise)

Preceding gene: 23100739

Following gene: 23100732

Centisome position: 94.25

GC content: 41.65

Gene sequence:

>1299_bases
ATGACAGTGAAAGAGTTACGTGAGCAATTAATTACGTGGAGAAGGGATTTTCACCAATATCCAGAAAAAGGTTTTTTAGA
AATGAGAACAGCATCTATTGTGGCTGATACGCTTCATCATCTTGGTTTTTCGCTGCAATTAGGTAAAGAGGTAATGTCAG
AAGCCGAGTGCATGGGAAAGCCGAATGACGAAGAAACGAAAGCACACATCGAGTGGGCACTTGATAATGGTGCACAGGAA
GCTTATATGGATCATTTTTCTGAAGGATATACTGGCGTGGTTGCTTCCATCGATACGAACAGACCAGGGCCAACAATAGC
ACTTCGGTTTGATATGGATGCTTTGCCGATCCATGAATCAGAAGAAGCCAATCATATTCCCAATAAAGAAGGGTATCGTT
CAAAGGTTCCCAATACAATGCACGCTTGTGGACATGATTCACATACTGCGATGGGATTAGGGCTTGCCACACTGATCGCT
GAAAACAAAGAAAAGCTACAAGGTAAGATAAAGTTGATTTTCCAACCAGCAGAAGAAGGGACTAGAGGGGCGAAGTCAAT
GACAAGTGCAGGAGTGGTTGATGATGTAGATTATTTCATTGCCACTCACATCGGAACAGGTGTCCCGCATCATCATTTTG
TTGCGGCGAATAATGGTTTTTTAGCTACTTCCAAGCTGGATATACAGTTTGATGGTATCGCATCTCACGCTGGCGGTAAG
CCCGAAGAAGGAAAGAATGCACTTTTAGCAGCAGCATCAGCCATATTGAATATACACGCTATTCCGCGCCATTCCAAGGG
CTCTTCGAGAGTAAACGTTGGAGAAATTCACGGTGGATCTGGAAGAAATATTATCGCTAGTCATGCGAATCTACTAGTTG
AAACACGGGGTGAAACCTCAGAAATCAATGACTATATCAAAGATCACGTGTTTTCGATAGTAGAAGGAGCAGCAAACATG
CATCAAATCAATTACGGTATAGATGTTGTTGGTGAAGCACTAGATTGCAAGTGTTCTGTAGAGTTGGCTGAAATCGTACA
AAAATGTGCTGATTCCCACGCTGTTATCGAAAAATCGATACTTGAAAGTAATGAGAGTGCAGGTTCTGAAGACGCTACGT
ATTTTATGGAGAAGGTACAGCAAAATGGTGGACTAGCAACCTACTGCGTTTTTGGTACAGAACTTGCTGCTGGTCATCAT
AATGAAAAATTTGATATAGATGAATCCTCCTTGCTCGTAGGGGTAGAAGTATTATATCAAGCTGTAAAAGAACTTGTAGT
AACTGAAATAAAAAGATAA

Upstream 100 bases:

>100_bases
CGTGGATTATACTTATTATGATATTTTATTATGTAGGAATTCCTTTTGGACCAGGAATCTACCCACATCTATAAATTAAC
GAAAACTTGGAGGACGTTTC

Downstream 100 bases:

>100_bases
AAGTAAAGAGAAAGAGCATAGTGGAATAAAAAGTTTTCGTCAAAAGACGTATGAATGCTAGAATAAGCGTAAGAAATATA
CGGAGACTCCTTGAAAATAA

Product: indole-3-acetyl-L-aspartic acid hydrolase

Products: NA

Alternate protein names: IAA-Asp hydrolase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MTVKELREQLITWRRDFHQYPEKGFLEMRTASIVADTLHHLGFSLQLGKEVMSEAECMGKPNDEETKAHIEWALDNGAQE
AYMDHFSEGYTGVVASIDTNRPGPTIALRFDMDALPIHESEEANHIPNKEGYRSKVPNTMHACGHDSHTAMGLGLATLIA
ENKEKLQGKIKLIFQPAEEGTRGAKSMTSAGVVDDVDYFIATHIGTGVPHHHFVAANNGFLATSKLDIQFDGIASHAGGK
PEEGKNALLAAASAILNIHAIPRHSKGSSRVNVGEIHGGSGRNIIASHANLLVETRGETSEINDYIKDHVFSIVEGAANM
HQINYGIDVVGEALDCKCSVELAEIVQKCADSHAVIEKSILESNESAGSEDATYFMEKVQQNGGLATYCVFGTELAAGHH
NEKFDIDESSLLVGVEVLYQAVKELVVTEIKR

Sequences:

>Translated_432_residues
MTVKELREQLITWRRDFHQYPEKGFLEMRTASIVADTLHHLGFSLQLGKEVMSEAECMGKPNDEETKAHIEWALDNGAQE
AYMDHFSEGYTGVVASIDTNRPGPTIALRFDMDALPIHESEEANHIPNKEGYRSKVPNTMHACGHDSHTAMGLGLATLIA
ENKEKLQGKIKLIFQPAEEGTRGAKSMTSAGVVDDVDYFIATHIGTGVPHHHFVAANNGFLATSKLDIQFDGIASHAGGK
PEEGKNALLAAASAILNIHAIPRHSKGSSRVNVGEIHGGSGRNIIASHANLLVETRGETSEINDYIKDHVFSIVEGAANM
HQINYGIDVVGEALDCKCSVELAEIVQKCADSHAVIEKSILESNESAGSEDATYFMEKVQQNGGLATYCVFGTELAAGHH
NEKFDIDESSLLVGVEVLYQAVKELVVTEIKR
>Mature_431_residues
TVKELREQLITWRRDFHQYPEKGFLEMRTASIVADTLHHLGFSLQLGKEVMSEAECMGKPNDEETKAHIEWALDNGAQEA
YMDHFSEGYTGVVASIDTNRPGPTIALRFDMDALPIHESEEANHIPNKEGYRSKVPNTMHACGHDSHTAMGLGLATLIAE
NKEKLQGKIKLIFQPAEEGTRGAKSMTSAGVVDDVDYFIATHIGTGVPHHHFVAANNGFLATSKLDIQFDGIASHAGGKP
EEGKNALLAAASAILNIHAIPRHSKGSSRVNVGEIHGGSGRNIIASHANLLVETRGETSEINDYIKDHVFSIVEGAANMH
QINYGIDVVGEALDCKCSVELAEIVQKCADSHAVIEKSILESNESAGSEDATYFMEKVQQNGGLATYCVFGTELAAGHHN
EKFDIDESSLLVGVEVLYQAVKELVVTEIKR

Specific function: Hydrolyzes indole-3-acetyl-N-aspartic acid to indole-3- acetic acid [H]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=416, Percent_Identity=50.4807692307692, Blast_Score=380, Evalue=1e-106,
Organism=Escherichia coli, GI1787598, Length=224, Percent_Identity=28.5714285714286, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: NA

Molecular weight: Translated: 46992; Mature: 46861

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKELREQLITWRRDFHQYPEKGFLEMRTASIVADTLHHLGFSLQLGKEVMSEAECMGK
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
PNDEETKAHIEWALDNGAQEAYMDHFSEGYTGVVASIDTNRPGPTIALRFDMDALPIHES
CCCCCHHEEEEEEECCCHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCC
EEANHIPNKEGYRSKVPNTMHACGHDSHTAMGLGLATLIAENKEKLQGKIKLIFQPAEEG
CCCCCCCCCCCCHHHCCCHHHHCCCCCCHHHHHHHHHHHHCCHHHHCCEEEEEEECCCCC
TRGAKSMTSAGVVDDVDYFIATHIGTGVPHHHFVAANNGFLATSKLDIQFDGIASHAGGK
CCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEEEEEECCCHHCCCCC
PEEGKNALLAAASAILNIHAIPRHSKGSSRVNVGEIHGGSGRNIIASHANLLVETRGETS
CCCCCHHHHHHHHHHHHEEECCCCCCCCCCEEEEEEECCCCCCEEECCCEEEEECCCCHH
EINDYIKDHVFSIVEGAANMHQINYGIDVVGEALDCKCSVELAEIVQKCADSHAVIEKSI
HHHHHHHHHHHHHHHCCHHHEEECCCHHHHCCCCCCEECCHHHHHHHHHCCHHHHHHHHH
LESNESAGSEDATYFMEKVQQNGGLATYCVFGTELAAGHHNEKFDIDESSLLVGVEVLYQ
HHCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHH
AVKELVVTEIKR
HHHHHHHHHCCC
>Mature Secondary Structure 
TVKELREQLITWRRDFHQYPEKGFLEMRTASIVADTLHHLGFSLQLGKEVMSEAECMGK
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
PNDEETKAHIEWALDNGAQEAYMDHFSEGYTGVVASIDTNRPGPTIALRFDMDALPIHES
CCCCCHHEEEEEEECCCHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCC
EEANHIPNKEGYRSKVPNTMHACGHDSHTAMGLGLATLIAENKEKLQGKIKLIFQPAEEG
CCCCCCCCCCCCHHHCCCHHHHCCCCCCHHHHHHHHHHHHCCHHHHCCEEEEEEECCCCC
TRGAKSMTSAGVVDDVDYFIATHIGTGVPHHHFVAANNGFLATSKLDIQFDGIASHAGGK
CCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEEEEEECCCHHCCCCC
PEEGKNALLAAASAILNIHAIPRHSKGSSRVNVGEIHGGSGRNIIASHANLLVETRGETS
CCCCCHHHHHHHHHHHHEEECCCCCCCCCCEEEEEEECCCCCCEEECCCEEEEECCCCHH
EINDYIKDHVFSIVEGAANMHQINYGIDVVGEALDCKCSVELAEIVQKCADSHAVIEKSI
HHHHHHHHHHHHHHHCCHHHEEECCCHHHHCCCCCCEECCHHHHHHHHHCCHHHHHHHHH
LESNESAGSEDATYFMEKVQQNGGLATYCVFGTELAAGHHNEKFDIDESSLLVGVEVLYQ
HHCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHH
AVKELVVTEIKR
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9747708 [H]