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The map label for this gene is mdeA [H]

Identifier: 23100094

GI number: 23100094

Start: 2705785

End: 2707080

Strand: Reverse

Name: mdeA [H]

Synonym: OB2639

Alternate gene names: 23100094

Gene position: 2707080-2705785 (Counterclockwise)

Preceding gene: 23100095

Following gene: 23100091

Centisome position: 74.56

GC content: 37.04

Gene sequence:

>1296_bases
TTGACGAATAATTTTGACGGAACATTAAATTTTAATACGATTGCGCAACATGGAGGACAGGAGTTTGATGGAGAAACAAA
TGCTAGGGCAGTACCTATTTATCAAACGACATCCTATGGTTTTGAAAGTCCGGAACAAGCTGCGTCTCTTTTTCAAATGC
AAGAAGCTGGCTACATTTATTCACGTAATGCAAATCCAACAAACCGTGTTCTTGAAAAGCGTATAGCTGAATTAGAGGGT
GGTATTGATGCATTTGCAGTTTCTTCTGGACAATCCGCTATTACAATTGCTTTATTAACGCTTGCGAAAGTAGGCGATGA
AATTGTGGCTACAAATGAGTTATATGGTGGTACTTATAATCTATTTTCGGAGACATTTAAACGCTTTGGTGTAACAGTTC
GATTTGTTGAAGGTACAAACTTCTCAGAAATATCCGCTTCCATTAACGAGAATACCAAGGCAGTCTTTACAGAAACAATT
GGAAATCCTGGGTTACAAATAGCTGATATTAAAGCATTAAGTTCTATCGCACATGAACATGGAATTCCTTTAGTCGTTGA
TTCTACCTTCACAACTCCATACTTACAGAAACCAATTTCATTTGGTGCCGATATTGTGATTCATTCGACCACTAAGTTTA
TTGGAGGGCATGGAACATCCATTGGCGGTATTATCGTTGATTCTGGAAAATTTAACTGGGATAATGGGAATTTCCCTGAA
TTTACAACACCTAAAAGTTCCTTAAACAATCAATCTTATGTCGAACTAAAAAATGACAATGCTTTTATTTCAAAAGCACG
ATTTGAATTAGGACATGATATTGGGACAGCATTATCACCATTTAATGGTTGGCTATTCATTCAAGGACTAGAATCACTAC
CACTACGTATGAAGCAACATGTATTAAATACAAAACAAATTGCAGCGTATTTAGCAGAACACGAACAGGTTACATGGGTG
AACTACCCTGGATTATCAGAGAATCATCAGTATGAACTTGCAAAAACCTATTTACCTAAAGGAACTGGAGCGATCTTAAC
ATTTGGTATAGAAGGAGGACATGAAGCGGCAAAATCGTTTATTCAATCGTTAAAGCTTTTATCACATGTTGCGAATATAG
GCGATTCAAAGACGCTTGTCATTCATCCAGCAAGTACAACACATGCAAGGTTAAGCCCAGAGGAACAAAAAAGAACAGGT
GTAACACCGGATCAGATTCGAATTTCAGTTGGATTAGAAGATGTCGAGGATATAAAATACGATATTCATCAAGCTCTTAA
AAAAAGTAGAGGCTAG

Upstream 100 bases:

>100_bases
TCAATATAAAAAGAAAGTGGTTTAATATAAATAGGAGTGAGCTAATTGCTCACCCCAACATTTGACTTAGAACCCATAGA
ATGAAGGGGGAGAAAAAAGA

Downstream 100 bases:

>100_bases
TTGTTATAGGATAGGTAATACAAAATTAGTTAGAAATGCAGAAGTCAGAGTCAAAAGCGCTAAGAATGAAATAATAAAAA
ACAGAACTACATCTTTTACG

Product: O-acetylhomoserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MTNNFDGTLNFNTIAQHGGQEFDGETNARAVPIYQTTSYGFESPEQAASLFQMQEAGYIYSRNANPTNRVLEKRIAELEG
GIDAFAVSSGQSAITIALLTLAKVGDEIVATNELYGGTYNLFSETFKRFGVTVRFVEGTNFSEISASINENTKAVFTETI
GNPGLQIADIKALSSIAHEHGIPLVVDSTFTTPYLQKPISFGADIVIHSTTKFIGGHGTSIGGIIVDSGKFNWDNGNFPE
FTTPKSSLNNQSYVELKNDNAFISKARFELGHDIGTALSPFNGWLFIQGLESLPLRMKQHVLNTKQIAAYLAEHEQVTWV
NYPGLSENHQYELAKTYLPKGTGAILTFGIEGGHEAAKSFIQSLKLLSHVANIGDSKTLVIHPASTTHARLSPEEQKRTG
VTPDQIRISVGLEDVEDIKYDIHQALKKSRG

Sequences:

>Translated_431_residues
MTNNFDGTLNFNTIAQHGGQEFDGETNARAVPIYQTTSYGFESPEQAASLFQMQEAGYIYSRNANPTNRVLEKRIAELEG
GIDAFAVSSGQSAITIALLTLAKVGDEIVATNELYGGTYNLFSETFKRFGVTVRFVEGTNFSEISASINENTKAVFTETI
GNPGLQIADIKALSSIAHEHGIPLVVDSTFTTPYLQKPISFGADIVIHSTTKFIGGHGTSIGGIIVDSGKFNWDNGNFPE
FTTPKSSLNNQSYVELKNDNAFISKARFELGHDIGTALSPFNGWLFIQGLESLPLRMKQHVLNTKQIAAYLAEHEQVTWV
NYPGLSENHQYELAKTYLPKGTGAILTFGIEGGHEAAKSFIQSLKLLSHVANIGDSKTLVIHPASTTHARLSPEEQKRTG
VTPDQIRISVGLEDVEDIKYDIHQALKKSRG
>Mature_430_residues
TNNFDGTLNFNTIAQHGGQEFDGETNARAVPIYQTTSYGFESPEQAASLFQMQEAGYIYSRNANPTNRVLEKRIAELEGG
IDAFAVSSGQSAITIALLTLAKVGDEIVATNELYGGTYNLFSETFKRFGVTVRFVEGTNFSEISASINENTKAVFTETIG
NPGLQIADIKALSSIAHEHGIPLVVDSTFTTPYLQKPISFGADIVIHSTTKFIGGHGTSIGGIIVDSGKFNWDNGNFPEF
TTPKSSLNNQSYVELKNDNAFISKARFELGHDIGTALSPFNGWLFIQGLESLPLRMKQHVLNTKQIAAYLAEHEQVTWVN
YPGLSENHQYELAKTYLPKGTGAILTFGIEGGHEAAKSFIQSLKLLSHVANIGDSKTLVIHPASTTHARLSPEEQKRTGV
TPDQIRISVGLEDVEDIKYDIHQALKKSRG

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=423, Percent_Identity=34.9881796690307, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI299473759, Length=423, Percent_Identity=32.387706855792, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI25453487, Length=422, Percent_Identity=30.0947867298578, Blast_Score=154, Evalue=2e-37,
Organism=Escherichia coli, GI1790375, Length=420, Percent_Identity=30.952380952381, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI1789383, Length=396, Percent_Identity=24.2424242424242, Blast_Score=123, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71980708, Length=403, Percent_Identity=31.7617866004963, Blast_Score=212, Evalue=4e-55,
Organism=Caenorhabditis elegans, GI17560056, Length=422, Percent_Identity=33.4123222748815, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17538055, Length=422, Percent_Identity=32.2274881516588, Blast_Score=199, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6323333, Length=433, Percent_Identity=48.0369515011547, Blast_Score=350, Evalue=2e-97,
Organism=Saccharomyces cerevisiae, GI6319307, Length=425, Percent_Identity=33.4117647058824, Blast_Score=216, Evalue=6e-57,
Organism=Saccharomyces cerevisiae, GI6321254, Length=443, Percent_Identity=25.9593679458239, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6321170, Length=318, Percent_Identity=26.1006289308176, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321904, Length=432, Percent_Identity=25, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19922554, Length=423, Percent_Identity=32.8605200945626, Blast_Score=209, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 47052; Mature: 46921

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNNFDGTLNFNTIAQHGGQEFDGETNARAVPIYQTTSYGFESPEQAASLFQMQEAGYIY
CCCCCCCEEEEHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCEEE
SRNANPTNRVLEKRIAELEGGIDAFAVSSGQSAITIALLTLAKVGDEIVATNELYGGTYN
ECCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEECCCCCCCHH
LFSETFKRFGVTVRFVEGTNFSEISASINENTKAVFTETIGNPGLQIADIKALSSIAHEH
HHHHHHHHHCCEEEEEECCCHHHHHHCCCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHC
GIPLVVDSTFTTPYLQKPISFGADIVIHSTTKFIGGHGTSIGGIIVDSGKFNWDNGNFPE
CCCEEEECCCCCHHHHHHHCCCCEEEEEECHHHCCCCCCCCCEEEEECCEEECCCCCCCC
FTTPKSSLNNQSYVELKNDNAFISKARFELGHDIGTALSPFNGWLFIQGLESLPLRMKQH
CCCCHHHCCCCEEEEECCCCCEEHHHHHHHCCHHHHHCCCCCCEEEEECHHHCHHHHHHH
VLNTKQIAAYLAEHEQVTWVNYPGLSENHQYELAKTYLPKGTGAILTFGIEGGHEAAKSF
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHCCCCCCEEEEEECCCCHHHHHHH
IQSLKLLSHVANIGDSKTLVIHPASTTHARLSPEEQKRTGVTPDQIRISVGLEDVEDIKY
HHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHCCCCHHHEEEEECCCHHHHHHH
DIHQALKKSRG
HHHHHHHHCCC
>Mature Secondary Structure 
TNNFDGTLNFNTIAQHGGQEFDGETNARAVPIYQTTSYGFESPEQAASLFQMQEAGYIY
CCCCCCEEEEHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCEEE
SRNANPTNRVLEKRIAELEGGIDAFAVSSGQSAITIALLTLAKVGDEIVATNELYGGTYN
ECCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEECCCCCCCHH
LFSETFKRFGVTVRFVEGTNFSEISASINENTKAVFTETIGNPGLQIADIKALSSIAHEH
HHHHHHHHHCCEEEEEECCCHHHHHHCCCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHC
GIPLVVDSTFTTPYLQKPISFGADIVIHSTTKFIGGHGTSIGGIIVDSGKFNWDNGNFPE
CCCEEEECCCCCHHHHHHHCCCCEEEEEECHHHCCCCCCCCCEEEEECCEEECCCCCCCC
FTTPKSSLNNQSYVELKNDNAFISKARFELGHDIGTALSPFNGWLFIQGLESLPLRMKQH
CCCCHHHCCCCEEEEECCCCCEEHHHHHHHCCHHHHHCCCCCCEEEEECHHHCHHHHHHH
VLNTKQIAAYLAEHEQVTWVNYPGLSENHQYELAKTYLPKGTGAILTFGIEGGHEAAKSF
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHCCCCCCEEEEEECCCCHHHHHHH
IQSLKLLSHVANIGDSKTLVIHPASTTHARLSPEEQKRTGVTPDQIRISVGLEDVEDIKY
HHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHCCCCHHHEEEEECCCHHHHHHH
DIHQALKKSRG
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]