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The map label for this gene is oppA [H]

Identifier: 23100066

GI number: 23100066

Start: 2672663

End: 2674504

Strand: Reverse

Name: oppA [H]

Synonym: OB2611

Alternate gene names: 23100066

Gene position: 2674504-2672663 (Counterclockwise)

Preceding gene: 23100067

Following gene: 23100065

Centisome position: 73.67

GC content: 37.89

Gene sequence:

>1842_bases
ATGAGAAAATCTAGTTTAAGCAAGCTTTTACTCGCAATGATGTTAATGCTATTGTTAGTATTAGCTGCATGTAATGGAGG
TTCTGATGATGCGGACTCAGAATCAGATGCAGGTTCAGACGCAGGATCAGATACAGAGGAAAATACGGATGAAGAATCTG
AAGAGGGTATTTATTCAATTGATGATTTTTCTACAGATAAGACAAGTGAAGGGGAAGCAATTGATGGTGGTACGTTAAAC
TTCGGTTTAGTTACTGATACTGTATTTGAAGGTACCTTAAACTGGAATTTCTATTCAGGTGCGCCAGACGCAGAAATTAT
TGGACTATTTGATGAACCCCTTCTAAGTACTGATAATAATTTTAACTACACACAAGATGGAGCAGCGGAGTGGGAAGTAT
CCGATGATAATAAAACATTTACATTTACAATTCGTGATAATGTAAATTGGCATGATGGAGAGCCTGTTACAGCAGAAGAC
TGGGCATATTCTTTTGAAGTAATTGGTCATCCTGATTATACTGGTGTTCGTTATGACTCTACAATGACTAATGTTGAGGG
AATGGAAGCTTATCATAACGGAGAAGCAGAAAGTATTTCTGGAATCGAAGTAGTTGATGAGAAAACGCTGAAAATCACAT
TTATTGAAGCTAATCCATCACTTATAGCAAGTGGAATTTGGCCATATGCAATGCCTAAACATATTTTTGAAGAAATTCCA
GTTGCAGAAATGGCTGAGTCAGATGCTGTACGTCAAAACCCAATAGGTATGGGGCCGTTTAAAGTTGATTCCATCGTACC
TGGAGAGTCTGTTACATTTACAGCAAACGAAGATTACTGGCAAGGAACACCAAAACTTGATGGTTTAACTTTAAAAGTAG
TAGCACCGGAAACAGTAGTTCAAGCTTTAGAAACTGGTGAGGTTGATTTGGTTGATTCATTCCCGACTGATCAATATCCA
GATGTTGCTGAAACAATGACAAATGTGGAGTTTTTAGGTCAAACAGATCTAGCGTATACTTATATTGGATTTAAATTGGG
TAAATGGGATGGAGATGCTGGTGAGGTAGTACCAAACCCAGATGCTAAAATGGCTGATGTGAATTTAAGAAAAGCAATGT
GGCATGCTGTTGATAATGATGCCGTAGGAAAGCAGTTCTATAATGGTTTACGTTGGAATGCAAATACATTAATCATTCCA
GCATATCCAGATTATCATGATCCTTCTATTGAAGCACCAACTTATGATCCAGACTTAGCTAATCAGATTTTAGATGAAGC
AGGATATGAAGATGTTACTGGTGATGGAATTCGTGAAAATCCAGATGGTGAAGAATTAGTGATTAACTTTGCTTCAATGT
CTGGTGGAGATACAGCTGAACCCATAGCTAACTATTACATTCAGTCTTGGGAAGCTGTAGGTTTGAAAGTACAATTATTA
GATGGACGTTTACAAGAGTTCAACACTTTCTATGATCGTGTAGAAGCTGACGATCCAGATATCGATATTTATCAAGGTGC
TTGGGGTACTGGTTCTGACGTAGATCCTTCAGGATTATATGGAAGAAATGCTCAGTTCAACTATACTCGTTATGCGAGTG
AAGAAAATGATGAATTACTAGCAGAAGGTGCATCAGAAGCATCTCTTGATATCGCACATCGTCAAGAAGTATACAAAGAT
TGGCAACAATTAATGGTAGATGAAATTCCTGTATTCCCAACACTTTATCGTGCAGTATTAGTTCCAGTTAACAATCGCGT
AACAGATTACTCTATTCAATGGAGCTGGAATGGTTGGCATGAAGTTGGCGTAACAGAAGAAACACCACAAACAGTAGAAT
AA

Upstream 100 bases:

>100_bases
TAAGAAATATTTTGGTACCTACATCAAAATTGATGTTGAGTATATAAGCCGGTAGTCCTTGCTGGCAAGACAGAAACAAT
AACAAAGGGAGGTTACAAGG

Downstream 100 bases:

>100_bases
TAGTATAAAGAAAAAACCCTACGTTGATAGGGTTTTTTCTTTATGCTTAAAATCAAATTTTATTTTAGTAGGTGAAAAAA
ATGATTCGTATCGAACTTGC

Product: oligopeptide ABC transporter oligopeptide-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 613; Mature: 613

Protein sequence:

>613_residues
MRKSSLSKLLLAMMLMLLLVLAACNGGSDDADSESDAGSDAGSDTEENTDEESEEGIYSIDDFSTDKTSEGEAIDGGTLN
FGLVTDTVFEGTLNWNFYSGAPDAEIIGLFDEPLLSTDNNFNYTQDGAAEWEVSDDNKTFTFTIRDNVNWHDGEPVTAED
WAYSFEVIGHPDYTGVRYDSTMTNVEGMEAYHNGEAESISGIEVVDEKTLKITFIEANPSLIASGIWPYAMPKHIFEEIP
VAEMAESDAVRQNPIGMGPFKVDSIVPGESVTFTANEDYWQGTPKLDGLTLKVVAPETVVQALETGEVDLVDSFPTDQYP
DVAETMTNVEFLGQTDLAYTYIGFKLGKWDGDAGEVVPNPDAKMADVNLRKAMWHAVDNDAVGKQFYNGLRWNANTLIIP
AYPDYHDPSIEAPTYDPDLANQILDEAGYEDVTGDGIRENPDGEELVINFASMSGGDTAEPIANYYIQSWEAVGLKVQLL
DGRLQEFNTFYDRVEADDPDIDIYQGAWGTGSDVDPSGLYGRNAQFNYTRYASEENDELLAEGASEASLDIAHRQEVYKD
WQQLMVDEIPVFPTLYRAVLVPVNNRVTDYSIQWSWNGWHEVGVTEETPQTVE

Sequences:

>Translated_613_residues
MRKSSLSKLLLAMMLMLLLVLAACNGGSDDADSESDAGSDAGSDTEENTDEESEEGIYSIDDFSTDKTSEGEAIDGGTLN
FGLVTDTVFEGTLNWNFYSGAPDAEIIGLFDEPLLSTDNNFNYTQDGAAEWEVSDDNKTFTFTIRDNVNWHDGEPVTAED
WAYSFEVIGHPDYTGVRYDSTMTNVEGMEAYHNGEAESISGIEVVDEKTLKITFIEANPSLIASGIWPYAMPKHIFEEIP
VAEMAESDAVRQNPIGMGPFKVDSIVPGESVTFTANEDYWQGTPKLDGLTLKVVAPETVVQALETGEVDLVDSFPTDQYP
DVAETMTNVEFLGQTDLAYTYIGFKLGKWDGDAGEVVPNPDAKMADVNLRKAMWHAVDNDAVGKQFYNGLRWNANTLIIP
AYPDYHDPSIEAPTYDPDLANQILDEAGYEDVTGDGIRENPDGEELVINFASMSGGDTAEPIANYYIQSWEAVGLKVQLL
DGRLQEFNTFYDRVEADDPDIDIYQGAWGTGSDVDPSGLYGRNAQFNYTRYASEENDELLAEGASEASLDIAHRQEVYKD
WQQLMVDEIPVFPTLYRAVLVPVNNRVTDYSIQWSWNGWHEVGVTEETPQTVE
>Mature_613_residues
MRKSSLSKLLLAMMLMLLLVLAACNGGSDDADSESDAGSDAGSDTEENTDEESEEGIYSIDDFSTDKTSEGEAIDGGTLN
FGLVTDTVFEGTLNWNFYSGAPDAEIIGLFDEPLLSTDNNFNYTQDGAAEWEVSDDNKTFTFTIRDNVNWHDGEPVTAED
WAYSFEVIGHPDYTGVRYDSTMTNVEGMEAYHNGEAESISGIEVVDEKTLKITFIEANPSLIASGIWPYAMPKHIFEEIP
VAEMAESDAVRQNPIGMGPFKVDSIVPGESVTFTANEDYWQGTPKLDGLTLKVVAPETVVQALETGEVDLVDSFPTDQYP
DVAETMTNVEFLGQTDLAYTYIGFKLGKWDGDAGEVVPNPDAKMADVNLRKAMWHAVDNDAVGKQFYNGLRWNANTLIIP
AYPDYHDPSIEAPTYDPDLANQILDEAGYEDVTGDGIRENPDGEELVINFASMSGGDTAEPIANYYIQSWEAVGLKVQLL
DGRLQEFNTFYDRVEADDPDIDIYQGAWGTGSDVDPSGLYGRNAQFNYTRYASEENDELLAEGASEASLDIAHRQEVYKD
WQQLMVDEIPVFPTLYRAVLVPVNNRVTDYSIQWSWNGWHEVGVTEETPQTVE

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=492, Percent_Identity=25, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1787052, Length=501, Percent_Identity=22.5548902195609, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1789397, Length=493, Percent_Identity=23.3265720081136, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI87081878, Length=491, Percent_Identity=21.7922606924644, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1787495, Length=498, Percent_Identity=22.6907630522088, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI1787762, Length=469, Percent_Identity=24.3070362473348, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 67789; Mature: 67789

Theoretical pI: Translated: 3.69; Mature: 3.69

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKSSLSKLLLAMMLMLLLVLAACNGGSDDADSESDAGSDAGSDTEENTDEESEEGIYSI
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEEE
DDFSTDKTSEGEAIDGGTLNFGLVTDTVFEGTLNWNFYSGAPDAEIIGLFDEPLLSTDNN
CCCCCCCCCCCCCCCCCEEEEEEEEEEEEECEEEEEEECCCCCCEEEEECCCCCCCCCCC
FNYTQDGAAEWEVSDDNKTFTFTIRDNVNWHDGEPVTAEDWAYSFEVIGHPDYTGVRYDS
CCCCCCCCEEEEECCCCEEEEEEEECCCCCCCCCCCCHHCCEEEEEEECCCCCCCEEECC
TMTNVEGMEAYHNGEAESISGIEVVDEKTLKITFIEANPSLIASGIWPYAMPKHIFEEIP
CCCCCCHHHHHCCCCCCCCCCEEEECCCEEEEEEEECCCCEEECCCCCCCCHHHHHHHCC
VAEMAESDAVRQNPIGMGPFKVDSIVPGESVTFTANEDYWQGTPKLDGLTLKVVAPETVV
HHHHHHCCCHHCCCCCCCCEEECCCCCCCCEEEECCCCCCCCCCCCCCEEEEEECHHHHH
QALETGEVDLVDSFPTDQYPDVAETMTNVEFLGQTDLAYTYIGFKLGKWDGDAGEVVPNP
HHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCCC
DAKMADVNLRKAMWHAVDNDAVGKQFYNGLRWNANTLIIPAYPDYHDPSIEAPTYDPDLA
CCCEECCHHHHHHHHHCCCCHHHHHHHCCCEECCCEEEEECCCCCCCCCCCCCCCCHHHH
NQILDEAGYEDVTGDGIRENPDGEELVINFASMSGGDTAEPIANYYIQSWEAVGLKVQLL
HHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEE
DGRLQEFNTFYDRVEADDPDIDIYQGAWGTGSDVDPSGLYGRNAQFNYTRYASEENDELL
CCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHH
AEGASEASLDIAHRQEVYKDWQQLMVDEIPVFPTLYRAVLVPVNNRVTDYSIQWSWNGWH
HCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHEEEEECCCCEEEEEEEEECCCEE
EVGVTEETPQTVE
ECCCCCCCCCCCC
>Mature Secondary Structure
MRKSSLSKLLLAMMLMLLLVLAACNGGSDDADSESDAGSDAGSDTEENTDEESEEGIYSI
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEEE
DDFSTDKTSEGEAIDGGTLNFGLVTDTVFEGTLNWNFYSGAPDAEIIGLFDEPLLSTDNN
CCCCCCCCCCCCCCCCCEEEEEEEEEEEEECEEEEEEECCCCCCEEEEECCCCCCCCCCC
FNYTQDGAAEWEVSDDNKTFTFTIRDNVNWHDGEPVTAEDWAYSFEVIGHPDYTGVRYDS
CCCCCCCCEEEEECCCCEEEEEEEECCCCCCCCCCCCHHCCEEEEEEECCCCCCCEEECC
TMTNVEGMEAYHNGEAESISGIEVVDEKTLKITFIEANPSLIASGIWPYAMPKHIFEEIP
CCCCCCHHHHHCCCCCCCCCCEEEECCCEEEEEEEECCCCEEECCCCCCCCHHHHHHHCC
VAEMAESDAVRQNPIGMGPFKVDSIVPGESVTFTANEDYWQGTPKLDGLTLKVVAPETVV
HHHHHHCCCHHCCCCCCCCEEECCCCCCCCEEEECCCCCCCCCCCCCCEEEEEECHHHHH
QALETGEVDLVDSFPTDQYPDVAETMTNVEFLGQTDLAYTYIGFKLGKWDGDAGEVVPNP
HHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCCC
DAKMADVNLRKAMWHAVDNDAVGKQFYNGLRWNANTLIIPAYPDYHDPSIEAPTYDPDLA
CCCEECCHHHHHHHHHCCCCHHHHHHHCCCEECCCEEEEECCCCCCCCCCCCCCCCHHHH
NQILDEAGYEDVTGDGIRENPDGEELVINFASMSGGDTAEPIANYYIQSWEAVGLKVQLL
HHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEE
DGRLQEFNTFYDRVEADDPDIDIYQGAWGTGSDVDPSGLYGRNAQFNYTRYASEENDELL
CCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHH
AEGASEASLDIAHRQEVYKDWQQLMVDEIPVFPTLYRAVLVPVNNRVTDYSIQWSWNGWH
HCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHEEEEECCCCEEEEEEEEECCCEE
EVGVTEETPQTVE
ECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8244921 [H]