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The map label for this gene is asnO [H]

Identifier: 23100063

GI number: 23100063

Start: 2668950

End: 2670794

Strand: Reverse

Name: asnO [H]

Synonym: OB2608

Alternate gene names: 23100063

Gene position: 2670794-2668950 (Counterclockwise)

Preceding gene: 23100064

Following gene: 23100062

Centisome position: 73.56

GC content: 37.94

Gene sequence:

>1845_bases
TTGTGTGGAATTACAGGTTTTGTGAATTGGGGTAAAACAGCAGACGAACAACGGTCTGTATTAGGAAAAATGACGGATAC
GTTATCACTACGTGGTCCAGATGATACACAACTTTGGACGAAAGGACATACAGGTTTTGGCCATAAGCGTTTGTCGGTTG
TAGATTTAGAGGGCGGAAAGCAACCAATGAGCAAGACAGTGAATGGAATAACCTATACAGTTTGTTATAACGGAGAACTA
TATAATACAGAGGATCTTCGTAAGGAGCTACTAAAAAGAGGATATACATTTTCTACAACATCCGATACAGAAGTATTATT
AACAAGTTATATCGAATGGAAAGAAGCATGTGTGGACCATTTTAATGGTATTTTTGCATTTGGAATTTGGGATGAGGAAA
AAGAGCAACTGTTTATGTCACGCGATCGATTAGGTGTAAAGCCTCTATTCTATGCAGAAAAAGCAAAAGGATTCATTTTT
GGTTCGGAGTTAAAAGCAATATTAGCTCATCCAGATATTTCTACAAAAGTTGATCGTACAGGACTGTCTGAAATATTTGG
CCTCGGACCATCGCGTACACCTGGTCATGGACTATTTAAAGACATTGATGAACTAAGAGCGGCACACTCTTTAACTTTCT
CAAAACATGGTTTGAAAGTATGGAGATATTGGAATATGGTAAGTAATCATCATACGGATTCGGAAGAAGAAACCGCAGAA
AAAGTGAGAGAGCTTTTTGTAGATGCAGTACAACGGCAACTCGTAGCGGATGTACCGGTCTCTACTTTGTTGTCAGGTGG
ATTGGATTCAAGTGCAATAACTGCAATTGCAGCAAATTATTATAAAGAAAAAGGTTATGGACCATTAACAACATTTTCTA
TCGATTATCAAGGAAATGAGCAACATTTCCAGAAAAGTAAATTCCAGCCTTCTAGTGACCGTGCTTGGATTGAAAAAATG
GTAGAAGGTTTCTCGACAGATCATCATGATGAGATAATTGCAGGTCAAGAACTTGCGATTTTACTAAAGGAAGCAGTGGA
GGTCCGTGATCAGCCAGGGCAAGCGGATATTGATTCTTCGCTACTTTGGTTTTGTCGTGAAATTAAACAGCATACCACAG
TAGCTTTATCTGGAGAATGTGCGGATGAAATATTTGGAGGATATCCTTGGTTCCATGATCCTGCTGCAGCTGGTAATAAT
TTTCCTTGGATACGTTCTTTAGATTCTAGAATTGATTTACTGCATGGGGATTGGAAGAAAAAATTAGATTTAGAGAGTTA
TGTACAAGAACAATATCAATCTACGATAGCTGAAACACCTCGTTTAGATGGAGAGGATAAAATAGATGCTAAACGTAGAG
AGTTATTTTATTTGAATATGCAATGGTTTATGTCCCAATTGTTAGATCGTAAGGACAGAATGAGTATGGGGGCAAGCTTA
GAAGTACGTGTGCCTTTTGCGGATCATCGTCTTGTGGAATATGTTTGGAACATTCCTTGGGATATCAAAATGGTAAATGG
TCATGAGAAAGGAATTCTACGTAAAGCAATGGAAGGTATTCTTCCAAATGAAGTGCTTTATCGAAAAAAGAGTCCCTATC
CACGAACTTTTCAACCGGAATATACAGAGCAAGTCAGTCAATGGATGAATCGAATACTGGTTGATCCGAATGCAAGATTA
TTTGAATTTATGAAAAAAGAAAAAGTAGAAGCAATAGTAAAAAGTAAAGGAAAAGAATTTAAGGATCCTTGGTATGGTCA
ACTAATGCGTGGCCCACAGTTGATTGCGCACCTTTGTCAAATTGATCATTGGTTGCGTCAGTATGATGTTGAAGTAGTAG
ATTGA

Upstream 100 bases:

>100_bases
TGAAATTCAATTGAAGAAAAATGTTTTTCTAATCACTTCATGGGCACTCATGCATAAAATGTGTAGGAGGCCTAAGGTTC
ATTAGAAAGGGTGGTTGGAT

Downstream 100 bases:

>100_bases
TGTAGTGATCACATGTACTGGTTTGTTGAAAAGACCAGTTAGGTTCTTGCAGGGGAAGAGGTTTAGGTGAGCTTCCGGAT
TAACTATTTAATGGAGGTTC

Product: asparagine synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 614; Mature: 614

Protein sequence:

>614_residues
MCGITGFVNWGKTADEQRSVLGKMTDTLSLRGPDDTQLWTKGHTGFGHKRLSVVDLEGGKQPMSKTVNGITYTVCYNGEL
YNTEDLRKELLKRGYTFSTTSDTEVLLTSYIEWKEACVDHFNGIFAFGIWDEEKEQLFMSRDRLGVKPLFYAEKAKGFIF
GSELKAILAHPDISTKVDRTGLSEIFGLGPSRTPGHGLFKDIDELRAAHSLTFSKHGLKVWRYWNMVSNHHTDSEEETAE
KVRELFVDAVQRQLVADVPVSTLLSGGLDSSAITAIAANYYKEKGYGPLTTFSIDYQGNEQHFQKSKFQPSSDRAWIEKM
VEGFSTDHHDEIIAGQELAILLKEAVEVRDQPGQADIDSSLLWFCREIKQHTTVALSGECADEIFGGYPWFHDPAAAGNN
FPWIRSLDSRIDLLHGDWKKKLDLESYVQEQYQSTIAETPRLDGEDKIDAKRRELFYLNMQWFMSQLLDRKDRMSMGASL
EVRVPFADHRLVEYVWNIPWDIKMVNGHEKGILRKAMEGILPNEVLYRKKSPYPRTFQPEYTEQVSQWMNRILVDPNARL
FEFMKKEKVEAIVKSKGKEFKDPWYGQLMRGPQLIAHLCQIDHWLRQYDVEVVD

Sequences:

>Translated_614_residues
MCGITGFVNWGKTADEQRSVLGKMTDTLSLRGPDDTQLWTKGHTGFGHKRLSVVDLEGGKQPMSKTVNGITYTVCYNGEL
YNTEDLRKELLKRGYTFSTTSDTEVLLTSYIEWKEACVDHFNGIFAFGIWDEEKEQLFMSRDRLGVKPLFYAEKAKGFIF
GSELKAILAHPDISTKVDRTGLSEIFGLGPSRTPGHGLFKDIDELRAAHSLTFSKHGLKVWRYWNMVSNHHTDSEEETAE
KVRELFVDAVQRQLVADVPVSTLLSGGLDSSAITAIAANYYKEKGYGPLTTFSIDYQGNEQHFQKSKFQPSSDRAWIEKM
VEGFSTDHHDEIIAGQELAILLKEAVEVRDQPGQADIDSSLLWFCREIKQHTTVALSGECADEIFGGYPWFHDPAAAGNN
FPWIRSLDSRIDLLHGDWKKKLDLESYVQEQYQSTIAETPRLDGEDKIDAKRRELFYLNMQWFMSQLLDRKDRMSMGASL
EVRVPFADHRLVEYVWNIPWDIKMVNGHEKGILRKAMEGILPNEVLYRKKSPYPRTFQPEYTEQVSQWMNRILVDPNARL
FEFMKKEKVEAIVKSKGKEFKDPWYGQLMRGPQLIAHLCQIDHWLRQYDVEVVD
>Mature_614_residues
MCGITGFVNWGKTADEQRSVLGKMTDTLSLRGPDDTQLWTKGHTGFGHKRLSVVDLEGGKQPMSKTVNGITYTVCYNGEL
YNTEDLRKELLKRGYTFSTTSDTEVLLTSYIEWKEACVDHFNGIFAFGIWDEEKEQLFMSRDRLGVKPLFYAEKAKGFIF
GSELKAILAHPDISTKVDRTGLSEIFGLGPSRTPGHGLFKDIDELRAAHSLTFSKHGLKVWRYWNMVSNHHTDSEEETAE
KVRELFVDAVQRQLVADVPVSTLLSGGLDSSAITAIAANYYKEKGYGPLTTFSIDYQGNEQHFQKSKFQPSSDRAWIEKM
VEGFSTDHHDEIIAGQELAILLKEAVEVRDQPGQADIDSSLLWFCREIKQHTTVALSGECADEIFGGYPWFHDPAAAGNN
FPWIRSLDSRIDLLHGDWKKKLDLESYVQEQYQSTIAETPRLDGEDKIDAKRRELFYLNMQWFMSQLLDRKDRMSMGASL
EVRVPFADHRLVEYVWNIPWDIKMVNGHEKGILRKAMEGILPNEVLYRKKSPYPRTFQPEYTEQVSQWMNRILVDPNARL
FEFMKKEKVEAIVKSKGKEFKDPWYGQLMRGPQLIAHLCQIDHWLRQYDVEVVD

Specific function: Asparagine synthetase involved in sporulation [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=409, Percent_Identity=28.1173594132029, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI168229252, Length=409, Percent_Identity=28.1173594132029, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI168229250, Length=409, Percent_Identity=28.1173594132029, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI296010848, Length=382, Percent_Identity=28.2722513089005, Blast_Score=117, Evalue=5e-26,
Organism=Homo sapiens, GI296010852, Length=311, Percent_Identity=26.6881028938907, Blast_Score=89, Evalue=2e-17,
Organism=Homo sapiens, GI296010850, Length=311, Percent_Identity=26.6881028938907, Blast_Score=89, Evalue=2e-17,
Organism=Escherichia coli, GI1786889, Length=548, Percent_Identity=27.3722627737226, Blast_Score=157, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17560178, Length=545, Percent_Identity=26.7889908256881, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI25147557, Length=527, Percent_Identity=26.1859582542694, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI71993933, Length=527, Percent_Identity=26.1859582542694, Blast_Score=125, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6321563, Length=567, Percent_Identity=25.7495590828924, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6325403, Length=570, Percent_Identity=25.4385964912281, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI45553209, Length=524, Percent_Identity=25.381679389313, Blast_Score=129, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 70517; Mature: 70517

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGITGFVNWGKTADEQRSVLGKMTDTLSLRGPDDTQLWTKGHTGFGHKRLSVVDLEGGK
CCCCCCCCCCCCCCHHHHHHHHHHHHHEEECCCCCCCEEECCCCCCCCCEEEEEECCCCC
QPMSKTVNGITYTVCYNGELYNTEDLRKELLKRGYTFSTTSDTEVLLTSYIEWKEACVDH
CHHHHHCCCEEEEEEECCCEECHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHH
FNGIFAFGIWDEEKEQLFMSRDRLGVKPLFYAEKAKGFIFGSELKAILAHPDISTKVDRT
CCCEEEEECCCCHHHHHHHHHHCCCCCCEEEHHHCCCCEEHHHHHHHHCCCCCCCHHHHC
GLSEIFGLGPSRTPGHGLFKDIDELRAAHSLTFSKHGLKVWRYWNMVSNHHTDSEEETAE
CHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHH
KVRELFVDAVQRQLVADVPVSTLLSGGLDSSAITAIAANYYKEKGYGPLTTFSIDYQGNE
HHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCH
QHFQKSKFQPSSDRAWIEKMVEGFSTDHHDEIIAGQELAILLKEAVEVRDQPGQADIDSS
HHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCHHHH
LLWFCREIKQHTTVALSGECADEIFGGYPWFHDPAAAGNNFPWIRSLDSRIDLLHGDWKK
HHHHHHHHHHHCEEEECCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHH
KLDLESYVQEQYQSTIAETPRLDGEDKIDAKRRELFYLNMQWFMSQLLDRKDRMSMGASL
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEEEEHHHHHHHHHCHHHHHHCCCCE
EVRVPFADHRLVEYVWNIPWDIKMVNGHEKGILRKAMEGILPNEVLYRKKSPYPRTFQPE
EEECCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCH
YTEQVSQWMNRILVDPNARLFEFMKKEKVEAIVKSKGKEFKDPWYGQLMRGPQLIAHLCQ
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHH
IDHWLRQYDVEVVD
HHHHHHHHCCEECC
>Mature Secondary Structure
MCGITGFVNWGKTADEQRSVLGKMTDTLSLRGPDDTQLWTKGHTGFGHKRLSVVDLEGGK
CCCCCCCCCCCCCCHHHHHHHHHHHHHEEECCCCCCCEEECCCCCCCCCEEEEEECCCCC
QPMSKTVNGITYTVCYNGELYNTEDLRKELLKRGYTFSTTSDTEVLLTSYIEWKEACVDH
CHHHHHCCCEEEEEEECCCEECHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHH
FNGIFAFGIWDEEKEQLFMSRDRLGVKPLFYAEKAKGFIFGSELKAILAHPDISTKVDRT
CCCEEEEECCCCHHHHHHHHHHCCCCCCEEEHHHCCCCEEHHHHHHHHCCCCCCCHHHHC
GLSEIFGLGPSRTPGHGLFKDIDELRAAHSLTFSKHGLKVWRYWNMVSNHHTDSEEETAE
CHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHH
KVRELFVDAVQRQLVADVPVSTLLSGGLDSSAITAIAANYYKEKGYGPLTTFSIDYQGNE
HHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCH
QHFQKSKFQPSSDRAWIEKMVEGFSTDHHDEIIAGQELAILLKEAVEVRDQPGQADIDSS
HHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCHHHH
LLWFCREIKQHTTVALSGECADEIFGGYPWFHDPAAAGNNFPWIRSLDSRIDLLHGDWKK
HHHHHHHHHHHCEEEECCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHH
KLDLESYVQEQYQSTIAETPRLDGEDKIDAKRRELFYLNMQWFMSQLLDRKDRMSMGASL
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEEEEHHHHHHHHHCHHHHHHCCCCE
EVRVPFADHRLVEYVWNIPWDIKMVNGHEKGILRKAMEGILPNEVLYRKKSPYPRTFQPE
EEECCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCH
YTEQVSQWMNRILVDPNARLFEFMKKEKVEAIVKSKGKEFKDPWYGQLMRGPQLIAHLCQ
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHH
IDHWLRQYDVEVVD
HHHHHHHHCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10498721 [H]