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The map label for this gene is lutR [H]

Identifier: 23100061

GI number: 23100061

Start: 2667589

End: 2668257

Strand: Reverse

Name: lutR [H]

Synonym: OB2606

Alternate gene names: 23100061

Gene position: 2668257-2667589 (Counterclockwise)

Preceding gene: 23100062

Following gene: 23100060

Centisome position: 73.5

GC content: 33.78

Gene sequence:

>669_bases
ATGTCAAAAAAAATAAGTGCTACAGTAACAGAATATATCGTTCAGCAATTAAAAAACGGACGATATAAGATTGGTGATAA
ATTACCGTCAGAAAGAGAATTTATGGAATTGCTTCATGTTGGTCGTTCATCCATCCGTGAATCATTGAACACATTAGAAG
ATATGGGAATTGTAGAGAAAAGAATGGGTATTGGCGTATTTGTCAAACAATTGAAAGTGAATCAACTTGCTGATGCGTAT
GTAATTGCATCCTTACTAGATAATATTTGTTTAACGGAGATGATGGATTTTCGGTTCATTTTAGAAGTGGAGATGGCTGG
AAGAGCTGCTTGTTTTGCAAGTAAACAAGATCTCATAAAAATGGAAGAAGCGATTTATTTACATCGAGAAGCAATTGACC
AAAAGACTTCTTTTATAAAAGCGGATGAACTATTTCATCAATCCATTATTGTAGCATCGAATAATCATATTATTAAGCAA
GCCTATCAAAGCCTTTCTGAGTTAGTTCAACATACACTTCGTCAAAAATGGAAAACGGTAGATTCAGTCAAATGTTTTGA
AGATCATGTAAAAATCTATGAAGCAATCAAAACACACCAAGAAATCAATGCGAAACAAATGATGATGGAACATTTACACG
CTCATTGGACGATTCCATCCAACTTCTAA

Upstream 100 bases:

>100_bases
AAAATATATGTAATATAACCTAACATATTAAGTTAGGATAAATTACAGTGAAGGAAAGCTATTCAATGATGGTAATTCGT
TTGCATAGGGAGGGGCTTGG

Downstream 100 bases:

>100_bases
TTACTCCTCTAAGAACTCCATGCGCTACAAAGTTTTTTTCATTAACGCTTAAAGAAAAGTATACATTCCTTACCCATAAC
GGGAGAGTTTAAAATGATAT

Product: transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MSKKISATVTEYIVQQLKNGRYKIGDKLPSEREFMELLHVGRSSIRESLNTLEDMGIVEKRMGIGVFVKQLKVNQLADAY
VIASLLDNICLTEMMDFRFILEVEMAGRAACFASKQDLIKMEEAIYLHREAIDQKTSFIKADELFHQSIIVASNNHIIKQ
AYQSLSELVQHTLRQKWKTVDSVKCFEDHVKIYEAIKTHQEINAKQMMMEHLHAHWTIPSNF

Sequences:

>Translated_222_residues
MSKKISATVTEYIVQQLKNGRYKIGDKLPSEREFMELLHVGRSSIRESLNTLEDMGIVEKRMGIGVFVKQLKVNQLADAY
VIASLLDNICLTEMMDFRFILEVEMAGRAACFASKQDLIKMEEAIYLHREAIDQKTSFIKADELFHQSIIVASNNHIIKQ
AYQSLSELVQHTLRQKWKTVDSVKCFEDHVKIYEAIKTHQEINAKQMMMEHLHAHWTIPSNF
>Mature_221_residues
SKKISATVTEYIVQQLKNGRYKIGDKLPSEREFMELLHVGRSSIRESLNTLEDMGIVEKRMGIGVFVKQLKVNQLADAYV
IASLLDNICLTEMMDFRFILEVEMAGRAACFASKQDLIKMEEAIYLHREAIDQKTSFIKADELFHQSIIVASNNHIIKQA
YQSLSELVQHTLRQKWKTVDSVKCFEDHVKIYEAIKTHQEINAKQMMMEHLHAHWTIPSNF

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790780, Length=226, Percent_Identity=29.2035398230088, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789621, Length=223, Percent_Identity=23.3183856502242, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789353, Length=211, Percent_Identity=28.436018957346, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI48994955, Length=198, Percent_Identity=24.2424242424242, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1790032, Length=224, Percent_Identity=26.3392857142857, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 25703; Mature: 25572

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKISATVTEYIVQQLKNGRYKIGDKLPSEREFMELLHVGRSSIRESLNTLEDMGIVEK
CCCCHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMGIGVFVKQLKVNQLADAYVIASLLDNICLTEMMDFRFILEVEMAGRAACFASKQDLIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
MEEAIYLHREAIDQKTSFIKADELFHQSIIVASNNHIIKQAYQSLSELVQHTLRQKWKTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
DSVKCFEDHVKIYEAIKTHQEINAKQMMMEHLHAHWTIPSNF
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SKKISATVTEYIVQQLKNGRYKIGDKLPSEREFMELLHVGRSSIRESLNTLEDMGIVEK
CCCHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMGIGVFVKQLKVNQLADAYVIASLLDNICLTEMMDFRFILEVEMAGRAACFASKQDLIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
MEEAIYLHREAIDQKTSFIKADELFHQSIIVASNNHIIKQAYQSLSELVQHTLRQKWKTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
DSVKCFEDHVKIYEAIKTHQEINAKQMMMEHLHAHWTIPSNF
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]