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The map label for this gene is comF [H]

Identifier: 23099972

GI number: 23099972

Start: 2573164

End: 2574588

Strand: Reverse

Name: comF [H]

Synonym: OB2517

Alternate gene names: 23099972

Gene position: 2574588-2573164 (Counterclockwise)

Preceding gene: 23099973

Following gene: 23099971

Centisome position: 70.91

GC content: 36.14

Gene sequence:

>1425_bases
ATGAAAGAAATAGATAGTAATTGTATTACATCCTCACCGTTATTCCTAACTTATTATTTTTCTAATAAACTACTATTAGA
AGAAGAGATTCCTTTCCCCAGCAAATGTCTTCAAACACTCCTATCCACTCAACAACTAATTTCCGTTAAAGCTATTATCC
GAAAAGGGTTATCTATTCAATGTGTACGATGTGGTAATAAAAATCGAAAGCTATTTTCTGTTTATCCTTGTAGCCGGTGT
AATCAGCAGAAGCATCTCTATTGTAGAAATTGTATTCAGATGGGTAAGATAAGTGAATGCAAACCTTTATATCTCTGGAA
TGGTACACAGGCAAAATACACATCGCATGCGAATGCATTAACCTGGGACGGTGAACTAACTTATGCTCAACAGACAGCAT
CAAACAGTTTAGTAAAAGCGATTCAAGGAAAAGAAAAAGAGTTTTTAATTTGGGCGGTTTGTGGATCCGGTAAAACAGAA
ATGCTATTTCCGGCTATCAATTATTGTATGCAACAAGGAAAACGTATTGCAATTGCTTCACCTAGAGTAGATGTTATTCG
TGAACTGTATCCGAGAATACAGTCAGCGTTTGAGAAGGTTACTGTGGAAGCAATGTATGGAAGTAGTGAAAGTAAATCTG
GTATGTCTCAAATTATATTGGCAACGACACATCAATTAATGCGATTTAGAGAGGCCTTTGATGTAATAGTAATTGATGAA
GTGGATGCGTTCCCTTTCTATTATGATAAGACATTAACTTACGCTGCAAATCATGCAAAAAAGCCTCATGCAATAAGCAT
TTACCTCACTGCAACCCCTCGTAAAGATCTACAAAACAGAATTCGACAACGAAAGCTTCCACATGTCTATGTACCTCTCC
GTTACCACGGTTATCCTTTACCTGAACCAACAATGAAAATGTCTCTTCGACTTCATCAATCCTTGTCTAACAAACAGTTC
CCTTCTATCTTCTATAAGTGGCTGTCCAAGCGAAATAATCCTAAAAGGCAACTATTAATTTTTGTCCCAACAGTTCGACT
AGCTAAAGATTTACAGAAAGAATTATGTGAAGAATTACTTAGCAATCGTATTATTAAAAATAAATCGGAATTTCAAGAAG
TTTATGCTGAAGATAAGCGCAGAGAAGAAAAGATAAATGACTTTCGTGTAAAGAAATTACAAGTATTACTTACTACAACA
ATCTTGGAAAGAGGAGTAACATTTCCATCTGTAGATGTAGCAGTCATGGATGCAGGCCATGAAGTTTTTGATGAAGCAGC
ACTTGTACAAATTGCAGGCCGAGCAGGGAGAAGTGCTACTGATCCAACTGGAGAAGTTGTATTCTTTCATAGCGGTAAAA
CAAAGGCAATGGAAAAAAGTAAGCAATTGATAGTTGATATGAACCGAAAAGGGAAAAAATTATGA

Upstream 100 bases:

>100_bases
AGCGGAGTATATTTCTGGAGTTTGTTTTTCAAAATTCGTGCTTATCTTTTGCGTAAATACTTTTTTTCCAGTCTCATTGA
TAATATACGGAGGTATATAG

Downstream 100 bases:

>100_bases
ATCAATGTCTGAGTTGCGGTCAATCTATGGATACAACAGTTTCCTGGGGGAATTGGTTAACCTTTAAACGAGATCAAGTC
ATTTGTTCTAACTGTTCACT

Product: late competence protein required for DNA uptake

Products: NA

Alternate protein names: ATP-dependent helicase ComFA [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MKEIDSNCITSSPLFLTYYFSNKLLLEEEIPFPSKCLQTLLSTQQLISVKAIIRKGLSIQCVRCGNKNRKLFSVYPCSRC
NQQKHLYCRNCIQMGKISECKPLYLWNGTQAKYTSHANALTWDGELTYAQQTASNSLVKAIQGKEKEFLIWAVCGSGKTE
MLFPAINYCMQQGKRIAIASPRVDVIRELYPRIQSAFEKVTVEAMYGSSESKSGMSQIILATTHQLMRFREAFDVIVIDE
VDAFPFYYDKTLTYAANHAKKPHAISIYLTATPRKDLQNRIRQRKLPHVYVPLRYHGYPLPEPTMKMSLRLHQSLSNKQF
PSIFYKWLSKRNNPKRQLLIFVPTVRLAKDLQKELCEELLSNRIIKNKSEFQEVYAEDKRREEKINDFRVKKLQVLLTTT
ILERGVTFPSVDVAVMDAGHEVFDEAALVQIAGRAGRSATDPTGEVVFFHSGKTKAMEKSKQLIVDMNRKGKKL

Sequences:

>Translated_474_residues
MKEIDSNCITSSPLFLTYYFSNKLLLEEEIPFPSKCLQTLLSTQQLISVKAIIRKGLSIQCVRCGNKNRKLFSVYPCSRC
NQQKHLYCRNCIQMGKISECKPLYLWNGTQAKYTSHANALTWDGELTYAQQTASNSLVKAIQGKEKEFLIWAVCGSGKTE
MLFPAINYCMQQGKRIAIASPRVDVIRELYPRIQSAFEKVTVEAMYGSSESKSGMSQIILATTHQLMRFREAFDVIVIDE
VDAFPFYYDKTLTYAANHAKKPHAISIYLTATPRKDLQNRIRQRKLPHVYVPLRYHGYPLPEPTMKMSLRLHQSLSNKQF
PSIFYKWLSKRNNPKRQLLIFVPTVRLAKDLQKELCEELLSNRIIKNKSEFQEVYAEDKRREEKINDFRVKKLQVLLTTT
ILERGVTFPSVDVAVMDAGHEVFDEAALVQIAGRAGRSATDPTGEVVFFHSGKTKAMEKSKQLIVDMNRKGKKL
>Mature_474_residues
MKEIDSNCITSSPLFLTYYFSNKLLLEEEIPFPSKCLQTLLSTQQLISVKAIIRKGLSIQCVRCGNKNRKLFSVYPCSRC
NQQKHLYCRNCIQMGKISECKPLYLWNGTQAKYTSHANALTWDGELTYAQQTASNSLVKAIQGKEKEFLIWAVCGSGKTE
MLFPAINYCMQQGKRIAIASPRVDVIRELYPRIQSAFEKVTVEAMYGSSESKSGMSQIILATTHQLMRFREAFDVIVIDE
VDAFPFYYDKTLTYAANHAKKPHAISIYLTATPRKDLQNRIRQRKLPHVYVPLRYHGYPLPEPTMKMSLRLHQSLSNKQF
PSIFYKWLSKRNNPKRQLLIFVPTVRLAKDLQKELCEELLSNRIIKNKSEFQEVYAEDKRREEKINDFRVKKLQVLLTTT
ILERGVTFPSVDVAVMDAGHEVFDEAALVQIAGRAGRSATDPTGEVVFFHSGKTKAMEKSKQLIVDMNRKGKKL

Specific function: Involved in transformation (competence for DNA uptake). Required for DNA uptake but not for binding. May provide the driving force for transport of DNA through an aqueous channel [H]

COG id: COG4098

COG function: function code L; Superfamily II DNA/RNA helicase required for DNA uptake (late competence protein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR006935 [H]

Pfam domain/function: PF00271 Helicase_C; PF04851 ResIII [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 54366; Mature: 54366

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEIDSNCITSSPLFLTYYFSNKLLLEEEIPFPSKCLQTLLSTQQLISVKAIIRKGLSIQ
CCCCCCCCCCCCCEEEEEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEE
CVRCGNKNRKLFSVYPCSRCNQQKHLYCRNCIQMGKISECKPLYLWNGTQAKYTSHANAL
EEECCCCCCEEEEECCCHHCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCEECCCCEE
TWDGELTYAQQTASNSLVKAIQGKEKEFLIWAVCGSGKTEMLFPAINYCMQQGKRIAIAS
EECCCEEEHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEHHHHHHHHHHCCCEEEEEC
PRVDVIRELYPRIQSAFEKVTVEAMYGSSESKSGMSQIILATTHQLMRFREAFDVIVIDE
CHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEC
VDAFPFYYDKTLTYAANHAKKPHAISIYLTATPRKDLQNRIRQRKLPHVYVPLRYHGYPL
CCCCCEEECCCHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHCCCCEEEEEEEECCCCC
PEPTMKMSLRLHQSLSNKQFPSIFYKWLSKRNNPKRQLLIFVPTVRLAKDLQKELCEELL
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHH
SNRIIKNKSEFQEVYAEDKRREEKINDFRVKKLQVLLTTTILERGVTFPSVDVAVMDAGH
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCH
EVFDEAALVQIAGRAGRSATDPTGEVVFFHSGKTKAMEKSKQLIVDMNRKGKKL
HHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHEEEECCCCCCCC
>Mature Secondary Structure
MKEIDSNCITSSPLFLTYYFSNKLLLEEEIPFPSKCLQTLLSTQQLISVKAIIRKGLSIQ
CCCCCCCCCCCCCEEEEEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEE
CVRCGNKNRKLFSVYPCSRCNQQKHLYCRNCIQMGKISECKPLYLWNGTQAKYTSHANAL
EEECCCCCCEEEEECCCHHCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCEECCCCEE
TWDGELTYAQQTASNSLVKAIQGKEKEFLIWAVCGSGKTEMLFPAINYCMQQGKRIAIAS
EECCCEEEHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEHHHHHHHHHHCCCEEEEEC
PRVDVIRELYPRIQSAFEKVTVEAMYGSSESKSGMSQIILATTHQLMRFREAFDVIVIDE
CHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEC
VDAFPFYYDKTLTYAANHAKKPHAISIYLTATPRKDLQNRIRQRKLPHVYVPLRYHGYPL
CCCCCEEECCCHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHCCCCEEEEEEEECCCCC
PEPTMKMSLRLHQSLSNKQFPSIFYKWLSKRNNPKRQLLIFVPTVRLAKDLQKELCEELL
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHH
SNRIIKNKSEFQEVYAEDKRREEKINDFRVKKLQVLLTTTILERGVTFPSVDVAVMDAGH
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCH
EVFDEAALVQIAGRAGRSATDPTGEVVFFHSGKTKAMEKSKQLIVDMNRKGKKL
HHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412657; 9384377 [H]