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The map label for this gene is ppaX

Identifier: 23099934

GI number: 23099934

Start: 2535913

End: 2536557

Strand: Reverse

Name: ppaX

Synonym: OB2479

Alternate gene names: 23099934

Gene position: 2536557-2535913 (Counterclockwise)

Preceding gene: 23099935

Following gene: 23099933

Centisome position: 69.87

GC content: 35.5

Gene sequence:

>645_bases
ATGAGCATTCGTACCATACTCTTTGATCTTGATGGGACCCTTATTGATACAAATACGTTAATTAAGGCTTCCTTTGAACA
TACATTTAAGGAATATAATCTAAACTTTAGTAATGAAGAAATTTTGAAGTTTAATGGTCCACCACTAGTAGATACGTTTA
ATAAAATTGATGAAACGAAAGCCGATAGGATGATTACAACTTTTCGTGAGCATAATATTCGTGAACATGATAATTTTGTC
ACGGCTTTTCCGCATGTATATGATACATTAGAAGAATTGCAGAATAGAAATATTTCTCTAGGAATAGTAAGTACAAAAAT
GCGTCATACGGTGCACATGGGACTTGAATTAACGGGGATATCTAAATTCTTTTCAACGATAATAACATATGATGATGTGA
CACATGCGAAGCCGCATCCAGAACCGGTACAGATGGCAATGCAAAAGCTAGGTGCACATCCGGAACATACGCTTATGGTG
GGAGATAATCACCATGATATTGTATCGGGTCAACGAGCAAATGTACAAACAGCAGCAGTTGCTTGGTCATTAAAAGACAC
GAACTACCTAAAAAGTTTTCATCCAGATTATATTATCGAAGATATTAAAGATATCATAACGATAGTAGGTGATTCGTTTG
CGTAA

Upstream 100 bases:

>100_bases
CCCTTGCTAATTATTCGATTATTGACGGCAATAATTCTAACAATGACCATCGCCTATTTCTGGAATAAACGTGATAAGAA
AAAAAGAAAGGAAATAATTC

Downstream 100 bases:

>100_bases
AACAGAGCGTTTTACAGTCAATCGTGCAAATTCATTATGGCAAATTTACGATACTGTTCCTTTTTGGAAGGTTATAAGAA
ATTTTATAGTAATTCAAATT

Product: pyrophosphatase PpaX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MSIRTILFDLDGTLIDTNTLIKASFEHTFKEYNLNFSNEEILKFNGPPLVDTFNKIDETKADRMITTFREHNIREHDNFV
TAFPHVYDTLEELQNRNISLGIVSTKMRHTVHMGLELTGISKFFSTIITYDDVTHAKPHPEPVQMAMQKLGAHPEHTLMV
GDNHHDIVSGQRANVQTAAVAWSLKDTNYLKSFHPDYIIEDIKDIITIVGDSFA

Sequences:

>Translated_214_residues
MSIRTILFDLDGTLIDTNTLIKASFEHTFKEYNLNFSNEEILKFNGPPLVDTFNKIDETKADRMITTFREHNIREHDNFV
TAFPHVYDTLEELQNRNISLGIVSTKMRHTVHMGLELTGISKFFSTIITYDDVTHAKPHPEPVQMAMQKLGAHPEHTLMV
GDNHHDIVSGQRANVQTAAVAWSLKDTNYLKSFHPDYIIEDIKDIITIVGDSFA
>Mature_213_residues
SIRTILFDLDGTLIDTNTLIKASFEHTFKEYNLNFSNEEILKFNGPPLVDTFNKIDETKADRMITTFREHNIREHDNFVT
AFPHVYDTLEELQNRNISLGIVSTKMRHTVHMGLELTGISKFFSTIITYDDVTHAKPHPEPVQMAMQKLGAHPEHTLMVG
DNHHDIVSGQRANVQTAAVAWSLKDTNYLKSFHPDYIIEDIKDIITIVGDSFA

Specific function: Hydrolyzes pyrophosphate formed during P-Ser-HPr dephosphorylation by HPrK/P. Might play a role in controlling the intracellular pyrophosphate pool

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. PpaX family

Homologues:

Organism=Escherichia coli, GI1789787, Length=241, Percent_Identity=28.2157676348548, Blast_Score=108, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPAX_OCEIH (Q8ENK3)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693400.1
- ProteinModelPortal:   Q8ENK3
- SMR:   Q8ENK3
- GeneID:   1015856
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2479
- NMPDR:   fig|221109.1.peg.2478
- HOGENOM:   HBG742904
- OMA:   IEEMINC
- ProtClustDB:   PRK13288
- BioCyc:   OIHE221109:OB2479-MONOMER
- BRENDA:   3.6.1.1
- HAMAP:   MF_01250
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- Gene3D:   G3DSA:3.40.50.1000
- TIGRFAMs:   TIGR01549
- TIGRFAMs:   TIGR01509

Pfam domain/function: PF00702 Hydrolase; SSF56784 SSF56784

EC number: =3.6.1.1

Molecular weight: Translated: 24442; Mature: 24311

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: ACT_SITE 9-9

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIRTILFDLDGTLIDTNTLIKASFEHTFKEYNLNFSNEEILKFNGPPLVDTFNKIDETK
CCCEEEEEECCCEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHH
ADRMITTFREHNIREHDNFVTAFPHVYDTLEELQNRNISLGIVSTKMRHTVHMGLELTGI
HHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHCCCEEEEEEHHHHHHHHCCCEEEEHH
SKFFSTIITYDDVTHAKPHPEPVQMAMQKLGAHPEHTLMVGDNHHDIVSGQRANVQTAAV
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHCCCCCCCCEEEEE
AWSLKDTNYLKSFHPDYIIEDIKDIITIVGDSFA
EEECCCCHHHHHCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SIRTILFDLDGTLIDTNTLIKASFEHTFKEYNLNFSNEEILKFNGPPLVDTFNKIDETK
CCEEEEEECCCEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHH
ADRMITTFREHNIREHDNFVTAFPHVYDTLEELQNRNISLGIVSTKMRHTVHMGLELTGI
HHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHCCCEEEEEEHHHHHHHHCCCEEEEHH
SKFFSTIITYDDVTHAKPHPEPVQMAMQKLGAHPEHTLMVGDNHHDIVSGQRANVQTAAV
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHCCCCCCCCEEEEE
AWSLKDTNYLKSFHPDYIIEDIKDIITIVGDSFA
EEECCCCHHHHHCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376