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The map label for this gene is rpoN [H]

Identifier: 23099896

GI number: 23099896

Start: 2496390

End: 2497682

Strand: Reverse

Name: rpoN [H]

Synonym: OB2441

Alternate gene names: 23099896

Gene position: 2497682-2496390 (Counterclockwise)

Preceding gene: 23099897

Following gene: 23099895

Centisome position: 68.8

GC content: 33.64

Gene sequence:

>1293_bases
TTGAAGCAAAAACTTGTAATCAATCAATCATTGCAGTGGAAAATGAATCAATCCTTATATCAATCTATTGAAATATTACA
ATTAACTGGGATGGAACTTTATGATTATATGAAACAAGTTGCTGAAGAGAACCCGCTCATTGAAGAAGTACAACCAATAG
ATAAGGAACTCATCTCCCAATTTCGGACAACCGATTATTCTATTGATGAAATGAATACAGCTGAACAGTCATTATATGAT
CAGTTAAAGTCAAATTTGTATGTTCTAGAATTGGAAGATAAATCTTTATTACCAGTAATTGTTTTTGGGATTGACTCATT
AAATGAGGATGGTTATTTAGAAATAACAATGGAAGACTGGGCATATTACTGTGAAGTAGAATTAACTACAGTGGAACGAG
CACTGTCATATATTCAATCATTGGAGCCGGCTGGTATTGGAGCTCGGAATCTTACAGAATGTATTCAGCTACAGTTGAAT
CAAATGAATATTGATGCTCCTTATATATCGTCGTTAATGAACGAAGGTATTACATTACTTGCAGAAGAAAACTCACATCA
AATTGCATTAAATTATGGGATTACCGAAGAAGCAGCACAGAAATTAATTGAATCAATCAAATTATGCCATCCAAAACCAG
GAAAGCTATTATCACCAACTAAAACGGAATTTATTATACCTGAAGCATCTATATATAAAGACGATACAGAGTGGAAAATT
TCCTTTTTTAATTGGTCCTCACCTGCAATACAAATAAACAGTGATTATAGTAAGCATGATTTAGACAGAGAGGCTGCTTC
ATTTGTGAAGAAAAAACAAAAAGAGGTGGATATGCTACAACAGGCAATAGATTATCGAAGCTCTACTTTAGAGAAAATAA
TACGAATAATAGTAGAGAAGCAACTGCCGTTTTTTGAAAGAGGTCCCAAAATGTTAAAACCGCTAACATTGAGTGAGCTT
TCCGCACAGTTGGACCTTCACATATCAACAATTAGTCGTGCCATTCGTCAGAAGTATGTTCAAACAGCTCATGGAGTTTT
ACCATTAAAGTATTTTCTACAACGTGAAGTGCAAAAAGGAGCAGGAGTTGCTTCCATAGTTATTAAACAATTACTTAAAG
AATTAGTAGATCTCGAAGATAAAAGTAAACCACTGTCAGATCAAAAAATTAAACAGTTGATGGAAGAACGTTATCAAATT
AAAATTGCACGTAGAACTATTGTGAAATACCGTGAAGAATTGCAAATTGCTTCTTCTGTTAAACGAAAAGGGGCTCAAGA
TAATGAAGAATGA

Upstream 100 bases:

>100_bases
AGAAGGTTATGTAAAGGACAAGTTTTGTTGAACTGTTCGTGAAGGTGACAGAAATGATAAGTAATGGCAAAATAAAAGTA
GAAAAAAGGGGTGAGATGAG

Downstream 100 bases:

>100_bases
AAATATATATTTTTATACAAAAGAAAATTGTCCACTATGTGTGGAAGCCGAAGAGTTATTGATCATGATGAATAAACAAG
ATATCCCAATTATTAAAAAA

Product: RNA polymerase sigma-54 factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MKQKLVINQSLQWKMNQSLYQSIEILQLTGMELYDYMKQVAEENPLIEEVQPIDKELISQFRTTDYSIDEMNTAEQSLYD
QLKSNLYVLELEDKSLLPVIVFGIDSLNEDGYLEITMEDWAYYCEVELTTVERALSYIQSLEPAGIGARNLTECIQLQLN
QMNIDAPYISSLMNEGITLLAEENSHQIALNYGITEEAAQKLIESIKLCHPKPGKLLSPTKTEFIIPEASIYKDDTEWKI
SFFNWSSPAIQINSDYSKHDLDREAASFVKKKQKEVDMLQQAIDYRSSTLEKIIRIIVEKQLPFFERGPKMLKPLTLSEL
SAQLDLHISTISRAIRQKYVQTAHGVLPLKYFLQREVQKGAGVASIVIKQLLKELVDLEDKSKPLSDQKIKQLMEERYQI
KIARRTIVKYREELQIASSVKRKGAQDNEE

Sequences:

>Translated_430_residues
MKQKLVINQSLQWKMNQSLYQSIEILQLTGMELYDYMKQVAEENPLIEEVQPIDKELISQFRTTDYSIDEMNTAEQSLYD
QLKSNLYVLELEDKSLLPVIVFGIDSLNEDGYLEITMEDWAYYCEVELTTVERALSYIQSLEPAGIGARNLTECIQLQLN
QMNIDAPYISSLMNEGITLLAEENSHQIALNYGITEEAAQKLIESIKLCHPKPGKLLSPTKTEFIIPEASIYKDDTEWKI
SFFNWSSPAIQINSDYSKHDLDREAASFVKKKQKEVDMLQQAIDYRSSTLEKIIRIIVEKQLPFFERGPKMLKPLTLSEL
SAQLDLHISTISRAIRQKYVQTAHGVLPLKYFLQREVQKGAGVASIVIKQLLKELVDLEDKSKPLSDQKIKQLMEERYQI
KIARRTIVKYREELQIASSVKRKGAQDNEE
>Mature_430_residues
MKQKLVINQSLQWKMNQSLYQSIEILQLTGMELYDYMKQVAEENPLIEEVQPIDKELISQFRTTDYSIDEMNTAEQSLYD
QLKSNLYVLELEDKSLLPVIVFGIDSLNEDGYLEITMEDWAYYCEVELTTVERALSYIQSLEPAGIGARNLTECIQLQLN
QMNIDAPYISSLMNEGITLLAEENSHQIALNYGITEEAAQKLIESIKLCHPKPGKLLSPTKTEFIIPEASIYKDDTEWKI
SFFNWSSPAIQINSDYSKHDLDREAASFVKKKQKEVDMLQQAIDYRSSTLEKIIRIIVEKQLPFFERGPKMLKPLTLSEL
SAQLDLHISTISRAIRQKYVQTAHGVLPLKYFLQREVQKGAGVASIVIKQLLKELVDLEDKSKPLSDQKIKQLMEERYQI
KIARRTIVKYREELQIASSVKRKGAQDNEE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the xylCAB operon and the xylS gene. The open complex (sigma-54 a

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=481, Percent_Identity=28.8981288981289, Blast_Score=204, Evalue=1e-53,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 49587; Mature: 49587

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQKLVINQSLQWKMNQSLYQSIEILQLTGMELYDYMKQVAEENPLIEEVQPIDKELISQ
CCCCEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FRTTDYSIDEMNTAEQSLYDQLKSNLYVLELEDKSLLPVIVFGIDSLNEDGYLEITMEDW
HHCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEECCCCCCCCEEEEEEECC
AYYCEVELTTVERALSYIQSLEPAGIGARNLTECIQLQLNQMNIDAPYISSLMNEGITLL
EEEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEE
AEENSHQIALNYGITEEAAQKLIESIKLCHPKPGKLLSPTKTEFIIPEASIYKDDTEWKI
EECCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCHHHCCCCCCEEE
SFFNWSSPAIQINSDYSKHDLDREAASFVKKKQKEVDMLQQAIDYRSSTLEKIIRIIVEK
EEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLPFFERGPKMLKPLTLSELSAQLDLHISTISRAIRQKYVQTAHGVLPLKYFLQREVQKG
CCCHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
AGVASIVIKQLLKELVDLEDKSKPLSDQKIKQLMEERYQIKIARRTIVKYREELQIASSV
CCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRKGAQDNEE
HHCCCCCCCC
>Mature Secondary Structure
MKQKLVINQSLQWKMNQSLYQSIEILQLTGMELYDYMKQVAEENPLIEEVQPIDKELISQ
CCCCEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FRTTDYSIDEMNTAEQSLYDQLKSNLYVLELEDKSLLPVIVFGIDSLNEDGYLEITMEDW
HHCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEECCCCCCCCEEEEEEECC
AYYCEVELTTVERALSYIQSLEPAGIGARNLTECIQLQLNQMNIDAPYISSLMNEGITLL
EEEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEE
AEENSHQIALNYGITEEAAQKLIESIKLCHPKPGKLLSPTKTEFIIPEASIYKDDTEWKI
EECCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCHHHCCCCCCEEE
SFFNWSSPAIQINSDYSKHDLDREAASFVKKKQKEVDMLQQAIDYRSSTLEKIIRIIVEK
EEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLPFFERGPKMLKPLTLSELSAQLDLHISTISRAIRQKYVQTAHGVLPLKYFLQREVQKG
CCCHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
AGVASIVIKQLLKELVDLEDKSKPLSDQKIKQLMEERYQIKIARRTIVKYREELQIASSV
CCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRKGAQDNEE
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2602128; 12534463 [H]