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The map label for this gene is sbcD [H]

Identifier: 23099706

GI number: 23099706

Start: 2293014

End: 2294180

Strand: Reverse

Name: sbcD [H]

Synonym: OB2251

Alternate gene names: 23099706

Gene position: 2294180-2293014 (Counterclockwise)

Preceding gene: 23099707

Following gene: 23099705

Centisome position: 63.19

GC content: 35.99

Gene sequence:

>1167_bases
ATGAAAATATTCCATACTGCAGATTGGCATCTGGGGAAGCTGGTTCAAGGAATATATATGACAGAAGACCAAAATTATAT
ACTAAATCAATTTGTTGCAGAAGTTGAAAGGGAACAACCGGATGTAGTAATTATTGCCGGAGATCTATATGATCGGGCAG
TTCCCCCAGTTGATGCAGTTCATTTATTAGATCAGATATTAGATAAAATAATTCACGGACTTCAGATCCCGGTATTAGCA
ATTGCTGGGAACCATGATAGTCCTGGAAGGTTACATTTTGGAAGTGGATTAATGAAAGAGAATGGTTACCATGTTATCGG
TAAAATATCCCGAGAACTAGAACCAGTCATTTTGAAAGACTCGTATGGAGAAGTGCATTTTCATTTCATTCCTTACGCAG
ATCCTAGTATGGTACGCAATACTTTTGAAAATGAAGACATACGTACACATGATGATGCTGCAAAATATATTACAGATAAA
ATTCGTGAAAATATGGACCCTCATGCAAGGCATGTCGCGATTGGACATGCTTTTGTAACGCCATTTGGTGAAGAACGTGA
GAATACAAGTGATTCAGAACGGCCACTTTCCATAGGTGGAGCAGAATATGTAAATGCACATCATTTTAAAATATTTAACT
ATACAGCACTTGGGCATCTCCACCAGGCGCATCATGTATTGGATGAGAAAATTCGCTACGCTGGATCTCCTTTAAAATAC
TCGATTTCTGAAGAAAATCATAAAAAGGGTTTTCTTATAGTTGACTTGGCTGAAAATGGGGAAGTCTCTGTTGAGAAGAA
GTTTTTAAATCCACGACGAGATATGCGTCGTGTGAAAATGACGATGGAAGAAATATTTAACGAAAAGCCTAGCGATGATT
ATGTATTTGTTCAATTAATGGATCAAACTCCAGTATTATCACCAATGGAAAAAATCCGGTCTATTTATCCAAATGCAATG
CATGTTGAACGAGTACTGATATCACCGATTTCTGTAAAAGAAGAAGATTCAATACCGAAGACAAAAATGAATGAAATGGA
ACTTTTCCATGCTTTTTATAAAGAAGTAAAAGGTGAAGGACCTTCCGAAGAGGCTTTAGAAATTATTAAAACAATAATGG
ATGATTTAAATAAAGAAGAAGAGCATATAGAAGTTAATACAAAATGA

Upstream 100 bases:

>100_bases
CAAGAGGGACTGTTATAAGAGAAAGCTAAAATTAGTAGTTAAATATTTTTAACTGAGAGTTTAATAATCGGAATAAAAAG
CACGTACAAGGGGTGAAATA

Downstream 100 bases:

>100_bases
CTGGAGGTGTGAACTTTGCGACCGTTGTATCTAAAACTAACAGCGTTTGGACCATATAAAGATACAGAAATTATTGATTT
CACAAAATTAGGTGACGTAC

Product: exonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MKIFHTADWHLGKLVQGIYMTEDQNYILNQFVAEVEREQPDVVIIAGDLYDRAVPPVDAVHLLDQILDKIIHGLQIPVLA
IAGNHDSPGRLHFGSGLMKENGYHVIGKISRELEPVILKDSYGEVHFHFIPYADPSMVRNTFENEDIRTHDDAAKYITDK
IRENMDPHARHVAIGHAFVTPFGEERENTSDSERPLSIGGAEYVNAHHFKIFNYTALGHLHQAHHVLDEKIRYAGSPLKY
SISEENHKKGFLIVDLAENGEVSVEKKFLNPRRDMRRVKMTMEEIFNEKPSDDYVFVQLMDQTPVLSPMEKIRSIYPNAM
HVERVLISPISVKEEDSIPKTKMNEMELFHAFYKEVKGEGPSEEALEIIKTIMDDLNKEEEHIEVNTK

Sequences:

>Translated_388_residues
MKIFHTADWHLGKLVQGIYMTEDQNYILNQFVAEVEREQPDVVIIAGDLYDRAVPPVDAVHLLDQILDKIIHGLQIPVLA
IAGNHDSPGRLHFGSGLMKENGYHVIGKISRELEPVILKDSYGEVHFHFIPYADPSMVRNTFENEDIRTHDDAAKYITDK
IRENMDPHARHVAIGHAFVTPFGEERENTSDSERPLSIGGAEYVNAHHFKIFNYTALGHLHQAHHVLDEKIRYAGSPLKY
SISEENHKKGFLIVDLAENGEVSVEKKFLNPRRDMRRVKMTMEEIFNEKPSDDYVFVQLMDQTPVLSPMEKIRSIYPNAM
HVERVLISPISVKEEDSIPKTKMNEMELFHAFYKEVKGEGPSEEALEIIKTIMDDLNKEEEHIEVNTK
>Mature_388_residues
MKIFHTADWHLGKLVQGIYMTEDQNYILNQFVAEVEREQPDVVIIAGDLYDRAVPPVDAVHLLDQILDKIIHGLQIPVLA
IAGNHDSPGRLHFGSGLMKENGYHVIGKISRELEPVILKDSYGEVHFHFIPYADPSMVRNTFENEDIRTHDDAAKYITDK
IRENMDPHARHVAIGHAFVTPFGEERENTSDSERPLSIGGAEYVNAHHFKIFNYTALGHLHQAHHVLDEKIRYAGSPLKY
SISEENHKKGFLIVDLAENGEVSVEKKFLNPRRDMRRVKMTMEEIFNEKPSDDYVFVQLMDQTPVLSPMEKIRSIYPNAM
HVERVLISPISVKEEDSIPKTKMNEMELFHAFYKEVKGEGPSEEALEIIKTIMDDLNKEEEHIEVNTK

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=276, Percent_Identity=26.8115942028986, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 44524; Mature: 44524

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFHTADWHLGKLVQGIYMTEDQNYILNQFVAEVEREQPDVVIIAGDLYDRAVPPVDAV
CCEEECCCCHHHHHHHHHEEECCCHHHHHHHHHHHHCCCCCEEEEECCHHHCCCCCHHHH
HLLDQILDKIIHGLQIPVLAIAGNHDSPGRLHFGSGLMKENGYHVIGKISRELEPVILKD
HHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEECCCCCCCCCCEEEEEHHCCCCCEEEEC
SYGEVHFHFIPYADPSMVRNTFENEDIRTHDDAAKYITDKIRENMDPHARHVAIGHAFVT
CCCCEEEEEECCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHEEEECHHEEC
PFGEERENTSDSERPLSIGGAEYVNAHHFKIFNYTALGHLHQAHHVLDEKIRYAGSPLKY
CCCCCCCCCCCCCCCEECCCHHHCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEE
SISEENHKKGFLIVDLAENGEVSVEKKFLNPRRDMRRVKMTMEEIFNEKPSDDYVFVQLM
ECCCCCCCCCEEEEEECCCCCEEEHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
DQTPVLSPMEKIRSIYPNAMHVERVLISPISVKEEDSIPKTKMNEMELFHAFYKEVKGEG
CCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
PSEEALEIIKTIMDDLNKEEEHIEVNTK
CCHHHHHHHHHHHHHCCCCCCCEEECCC
>Mature Secondary Structure
MKIFHTADWHLGKLVQGIYMTEDQNYILNQFVAEVEREQPDVVIIAGDLYDRAVPPVDAV
CCEEECCCCHHHHHHHHHEEECCCHHHHHHHHHHHHCCCCCEEEEECCHHHCCCCCHHHH
HLLDQILDKIIHGLQIPVLAIAGNHDSPGRLHFGSGLMKENGYHVIGKISRELEPVILKD
HHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEECCCCCCCCCCEEEEEHHCCCCCEEEEC
SYGEVHFHFIPYADPSMVRNTFENEDIRTHDDAAKYITDKIRENMDPHARHVAIGHAFVT
CCCCEEEEEECCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHEEEECHHEEC
PFGEERENTSDSERPLSIGGAEYVNAHHFKIFNYTALGHLHQAHHVLDEKIRYAGSPLKY
CCCCCCCCCCCCCCCEECCCHHHCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEE
SISEENHKKGFLIVDLAENGEVSVEKKFLNPRRDMRRVKMTMEEIFNEKPSDDYVFVQLM
ECCCCCCCCCEEEEEECCCCCEEEHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
DQTPVLSPMEKIRSIYPNAMHVERVLISPISVKEEDSIPKTKMNEMELFHAFYKEVKGEG
CCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
PSEEALEIIKTIMDDLNKEEEHIEVNTK
CCHHHHHHHHHHHHHCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA