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The map label for this gene is pksG [H]

Identifier: 23099703

GI number: 23099703

Start: 2287884

End: 2289050

Strand: Reverse

Name: pksG [H]

Synonym: OB2248

Alternate gene names: 23099703

Gene position: 2289050-2287884 (Counterclockwise)

Preceding gene: 23099704

Following gene: 23099702

Centisome position: 63.05

GC content: 37.53

Gene sequence:

>1167_bases
TTGAAGATTGGAATAGATAAGATTGGTTTCTATGCTCCGCATTTATATTTAGACATGAATGAATTAGCTGAAGAACGTGG
AGTCGAGCCAGAAAAATTCACAATAGGAATCGGCCAAGAAAAAATGGCCGTACCGCCAATTACACAAGACACAGTTACGT
TAGCTGCGAATGCGGCTGCACAGATATTAGATGAAACGGATAAGCAAGATATTGACATGGTGATTTTTGGAACAGAGTCA
GGTATCGATCATTCCAAATCAGCTGCGGTAAATGTGCACCAACTATTAGGATTACAACCGCATGCACGTGCTATTGAATT
AAAACAAGCATGTTATGGTGCTACAGCAGGTATTCAGATGGCGAGAGGACATATAGCTTTAAATCCGAATCGTAAAGTAT
TAGTGTTAGGATCAGATATTGCACGATACGGATTAAGAACACCAGGAGAATCAACACAGGGAGCGGGTGCGGTTGCGTTA
TTAATTTCCGCTGATCCAAGTATTATGGCAATTGAACAAGAAAGTACCTACTATACGGATGATATTATGGATTTCTGGAG
ACCGGTTTACTCAGACATAGCATTTGTTGATGGTAAGTACTCCAATGAGCAGTATATTGCATTTTTTCAAAAAGTTTGGA
ATGCATATAAAGAGCAGACAAATCGTGGTTTAGAAGATTTTGCAGCAATTTGTTTTCATTTACCATATACGAAAATGGGC
TTAAAGGCCTTGCGTACTGTATTGGATGAGGTAGAAGAAAATACTCAAACGCATCTGAAGGAACAATATCGATTAAGTAC
CCAATATAATCGCAATATCGGTAATATCTATACAGGTTCGCTTTATTTGAGTTTACTTTCATTATTGGAAAACAGTAATG
AATTAACAGCTGGTGACAAAATAGGTATGTATAGCTACGGATCTGGTGCTGTTGGGGAATTTTTCTCTGGTGTATTACAA
GAAGGTTTTGAAAAACAATTACATCAAACAAAACATAATGCGATGTTTGAGAAAAGACAAAAAATATCGGTATCTGAATA
TGAAAAAATCTTCGAACAACAAATCCCAACGGACGGTTCAGTTACGGAATGGTCAACAAAAGAGGACCCGGCTGAGATTT
GCCTAACAGGGATACAAAATCATCAACGTATATATCAAGTGAAATAA

Upstream 100 bases:

>100_bases
TTTTTTGAGATTGATTATATTATCATTTAAGTATTGGATTTCGAGCATTTTATTAGAAATATATGAATAAGACAAGTTGT
AATAGTGAAGGAGAATGACA

Downstream 100 bases:

>100_bases
TTGATTTAAAAAATATACTAAAATTGTACGGAAGTCCAATTCTTTTATAAGAGTTGGGCTTTTTGACTTTCAAGTAGAAT
TTAACTATACCTCCTTTAAG

Product: hydroxymethylglutaryl-CoA synthase

Products: (S)-3-hydroxy-3-methylglutaryl-CoA; CoA

Alternate protein names: HMG synthase [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MKIGIDKIGFYAPHLYLDMNELAEERGVEPEKFTIGIGQEKMAVPPITQDTVTLAANAAAQILDETDKQDIDMVIFGTES
GIDHSKSAAVNVHQLLGLQPHARAIELKQACYGATAGIQMARGHIALNPNRKVLVLGSDIARYGLRTPGESTQGAGAVAL
LISADPSIMAIEQESTYYTDDIMDFWRPVYSDIAFVDGKYSNEQYIAFFQKVWNAYKEQTNRGLEDFAAICFHLPYTKMG
LKALRTVLDEVEENTQTHLKEQYRLSTQYNRNIGNIYTGSLYLSLLSLLENSNELTAGDKIGMYSYGSGAVGEFFSGVLQ
EGFEKQLHQTKHNAMFEKRQKISVSEYEKIFEQQIPTDGSVTEWSTKEDPAEICLTGIQNHQRIYQVK

Sequences:

>Translated_388_residues
MKIGIDKIGFYAPHLYLDMNELAEERGVEPEKFTIGIGQEKMAVPPITQDTVTLAANAAAQILDETDKQDIDMVIFGTES
GIDHSKSAAVNVHQLLGLQPHARAIELKQACYGATAGIQMARGHIALNPNRKVLVLGSDIARYGLRTPGESTQGAGAVAL
LISADPSIMAIEQESTYYTDDIMDFWRPVYSDIAFVDGKYSNEQYIAFFQKVWNAYKEQTNRGLEDFAAICFHLPYTKMG
LKALRTVLDEVEENTQTHLKEQYRLSTQYNRNIGNIYTGSLYLSLLSLLENSNELTAGDKIGMYSYGSGAVGEFFSGVLQ
EGFEKQLHQTKHNAMFEKRQKISVSEYEKIFEQQIPTDGSVTEWSTKEDPAEICLTGIQNHQRIYQVK
>Mature_388_residues
MKIGIDKIGFYAPHLYLDMNELAEERGVEPEKFTIGIGQEKMAVPPITQDTVTLAANAAAQILDETDKQDIDMVIFGTES
GIDHSKSAAVNVHQLLGLQPHARAIELKQACYGATAGIQMARGHIALNPNRKVLVLGSDIARYGLRTPGESTQGAGAVAL
LISADPSIMAIEQESTYYTDDIMDFWRPVYSDIAFVDGKYSNEQYIAFFQKVWNAYKEQTNRGLEDFAAICFHLPYTKMG
LKALRTVLDEVEENTQTHLKEQYRLSTQYNRNIGNIYTGSLYLSLLSLLENSNELTAGDKIGMYSYGSGAVGEFFSGVLQ
EGFEKQLHQTKHNAMFEKRQKISVSEYEKIFEQQIPTDGSVTEWSTKEDPAEICLTGIQNHQRIYQVK

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. It catalyzes the aldol condensation between the acetyl group attached to the acyl-carrier-protein AcpK (Ac- AcpK) and

COG id: COG3425

COG function: function code I; 3-hydroxy-3-methylglutaryl CoA synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA synthase family [H]

Homologues:

Organism=Homo sapiens, GI148298764, Length=370, Percent_Identity=29.1891891891892, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI148298677, Length=370, Percent_Identity=29.1891891891892, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI5031751, Length=372, Percent_Identity=26.8817204301075, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17560126, Length=361, Percent_Identity=27.9778393351801, Blast_Score=144, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6323509, Length=368, Percent_Identity=27.1739130434783, Blast_Score=113, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24654139, Length=410, Percent_Identity=26.5853658536585, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24654137, Length=410, Percent_Identity=26.5853658536585, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24654133, Length=410, Percent_Identity=26.5853658536585, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24654135, Length=410, Percent_Identity=26.5853658536585, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI17933694, Length=410, Percent_Identity=26.5853658536585, Blast_Score=119, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013746
- InterPro:   IPR013528
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF08540 HMG_CoA_synt_C; PF01154 HMG_CoA_synt_N [H]

EC number: 2.3.3.10

Molecular weight: Translated: 43378; Mature: 43378

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGIDKIGFYAPHLYLDMNELAEERGVEPEKFTIGIGQEKMAVPPITQDTVTLAANAAA
CCCCCCHHCCCCCEEEEEHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHH
QILDETDKQDIDMVIFGTESGIDHSKSAAVNVHQLLGLQPHARAIELKQACYGATAGIQM
HHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHCHHE
ARGHIALNPNRKVLVLGSDIARYGLRTPGESTQGAGAVALLISADPSIMAIEQESTYYTD
ECCEEEECCCCEEEEECCHHHHHCCCCCCCCCCCCCEEEEEEECCCCEEEEECCCCEEHH
DIMDFWRPVYSDIAFVDGKYSNEQYIAFFQKVWNAYKEQTNRGLEDFAAICFHLPYTKMG
HHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHH
LKALRTVLDEVEENTQTHLKEQYRLSTQYNRNIGNIYTGSLYLSLLSLLENSNELTAGDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCC
IGMYSYGSGAVGEFFSGVLQEGFEKQLHQTKHNAMFEKRQKISVSEYEKIFEQQIPTDGS
CCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
VTEWSTKEDPAEICLTGIQNHQRIYQVK
CCCCCCCCCHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure
MKIGIDKIGFYAPHLYLDMNELAEERGVEPEKFTIGIGQEKMAVPPITQDTVTLAANAAA
CCCCCCHHCCCCCEEEEEHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHH
QILDETDKQDIDMVIFGTESGIDHSKSAAVNVHQLLGLQPHARAIELKQACYGATAGIQM
HHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHCHHE
ARGHIALNPNRKVLVLGSDIARYGLRTPGESTQGAGAVALLISADPSIMAIEQESTYYTD
ECCEEEECCCCEEEEECCHHHHHCCCCCCCCCCCCCEEEEEEECCCCEEEEECCCCEEHH
DIMDFWRPVYSDIAFVDGKYSNEQYIAFFQKVWNAYKEQTNRGLEDFAAICFHLPYTKMG
HHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHH
LKALRTVLDEVEENTQTHLKEQYRLSTQYNRNIGNIYTGSLYLSLLSLLENSNELTAGDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCC
IGMYSYGSGAVGEFFSGVLQEGFEKQLHQTKHNAMFEKRQKISVSEYEKIFEQQIPTDGS
CCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
VTEWSTKEDPAEICLTGIQNHQRIYQVK
CCCCCCCCCHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acetyl-CoA; H2O; acetoacetyl-CoA

Specific reaction: acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377 [H]