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The map label for this gene is tyrS

Identifier: 23099673

GI number: 23099673

Start: 2254798

End: 2256084

Strand: Reverse

Name: tyrS

Synonym: OB2218

Alternate gene names: 23099673

Gene position: 2256084-2254798 (Counterclockwise)

Preceding gene: 23099675

Following gene: 23099672

Centisome position: 62.14

GC content: 35.51

Gene sequence:

>1287_bases
ATGGATATTTTACAGGATTTAGAAAGCAGAGGTTTAATTCATCAAACAACTGACCGTGAAAGGTTAGAGAAACATTTACA
AAATAATCAAGTAACATTATATTGTGGATTTGACCCAACAGCAGATAGTTTGCATATTGGACATTTAGTTCCGTTGACGA
TGTTAAAACGATTTCAACAAGCTGGCCATAAACCTATTGCTCTTGTTGGTGGGGGAACAGGGATGATTGGAGATCCTAGT
GGACGATCAAGTGAAAGAAGTCTAAATGCTCCAGAAGTGGTACATGGATTCACTTCAAAACTACAACAGCAAATTGCAAA
GTTAGTTGATATGGAAAGTAATGCAAGCGAAAATACGGTAGTAGCACGTAATAATCATGACTGGTTAGGAAATATGACAA
TAATTGATTTTCTACGTGATGCAGGGAAGCATTTTGGCATAAATTATATGTTAGCAAAGGAATCTGTATCAGCAAGAATT
GAACAAGGTATTACTTTTACAGAATTCAGTTATATGATTTTACAATCATTAGACTACTTAAAACTTTATGAGGAAGAAAA
CTGTACATTACAAATTGGTGGAAGTGACCAGTGGGGGAATATCACTGCAGGTATGGAACTCATCCGCCGTTCACGAGAAA
ATCTGGATGAAAATATTGAAGTCTTCGGTTTAACAGTGCCACTGATCACAAAAGCAGATGGAACGAAATTTGGTAAAACA
GCTGGCAATGCTATTTGGCTTGATGCATCGAAAACGTCACCATACGAATTTTATCAATTTTGGTTTAATACGGATGATCG
TGATGTTATTCGTTTTATTCGCTATTTTACCTTCTTATCGGTAGAAGAAATAACTGAACTGGAAAAAGAAGTAGAAACAC
AACCAGAAAAGCGCGTTGCCCAAAAACGTTTAGCTGAAGAAATGACAAAAACCGTTCATGATGAAGCTGCATTAGAACAA
GCGAAGCGTATTACAAATGCATTGTTTAGTGGAGATATTAAACAATTAAACGTGGAAGAGGTAGAACAAGCATTTAAAGA
TGTTCCAAACCATATTATTACACGTGAACCTGTTGGTTTAATCGATTTACTTGTCGATGCGAAAATATCATCTTCAAAAC
GACAAGCAAGAGAAGATGTAAAAAATGGTGCAATTTATATTAACGGTGAACGCGTGCAGGATATGAATTATCATGTAGAA
GAAAAAGATCATTTGGGTAATAAATTCACTGTTGTTCGTCGCGGGAAGAAAAAGTATTTCCTTATTCAAGTAGAATCAAT
TTCATAA

Upstream 100 bases:

>100_bases
TCCAGCAACAAGGGTGGTACCGCGAATACAACTCGTCCCTTCCATTTTAGGAGGAGACGAGTTTTTTATTTGAAAAATAT
AAATTTTGGAGTGAGATTAC

Downstream 100 bases:

>100_bases
TAGGATATTTTAGTTAAACCCAAATGTTGACTGTTTTGATTTTCATTACAGACGGTCGCTTCATAGTAGGCACAGCCTCA
GCTAACTTAATGACCTTTAG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MDILQDLESRGLIHQTTDRERLEKHLQNNQVTLYCGFDPTADSLHIGHLVPLTMLKRFQQAGHKPIALVGGGTGMIGDPS
GRSSERSLNAPEVVHGFTSKLQQQIAKLVDMESNASENTVVARNNHDWLGNMTIIDFLRDAGKHFGINYMLAKESVSARI
EQGITFTEFSYMILQSLDYLKLYEEENCTLQIGGSDQWGNITAGMELIRRSRENLDENIEVFGLTVPLITKADGTKFGKT
AGNAIWLDASKTSPYEFYQFWFNTDDRDVIRFIRYFTFLSVEEITELEKEVETQPEKRVAQKRLAEEMTKTVHDEAALEQ
AKRITNALFSGDIKQLNVEEVEQAFKDVPNHIITREPVGLIDLLVDAKISSSKRQAREDVKNGAIYINGERVQDMNYHVE
EKDHLGNKFTVVRRGKKKYFLIQVESIS

Sequences:

>Translated_428_residues
MDILQDLESRGLIHQTTDRERLEKHLQNNQVTLYCGFDPTADSLHIGHLVPLTMLKRFQQAGHKPIALVGGGTGMIGDPS
GRSSERSLNAPEVVHGFTSKLQQQIAKLVDMESNASENTVVARNNHDWLGNMTIIDFLRDAGKHFGINYMLAKESVSARI
EQGITFTEFSYMILQSLDYLKLYEEENCTLQIGGSDQWGNITAGMELIRRSRENLDENIEVFGLTVPLITKADGTKFGKT
AGNAIWLDASKTSPYEFYQFWFNTDDRDVIRFIRYFTFLSVEEITELEKEVETQPEKRVAQKRLAEEMTKTVHDEAALEQ
AKRITNALFSGDIKQLNVEEVEQAFKDVPNHIITREPVGLIDLLVDAKISSSKRQAREDVKNGAIYINGERVQDMNYHVE
EKDHLGNKFTVVRRGKKKYFLIQVESIS
>Mature_428_residues
MDILQDLESRGLIHQTTDRERLEKHLQNNQVTLYCGFDPTADSLHIGHLVPLTMLKRFQQAGHKPIALVGGGTGMIGDPS
GRSSERSLNAPEVVHGFTSKLQQQIAKLVDMESNASENTVVARNNHDWLGNMTIIDFLRDAGKHFGINYMLAKESVSARI
EQGITFTEFSYMILQSLDYLKLYEEENCTLQIGGSDQWGNITAGMELIRRSRENLDENIEVFGLTVPLITKADGTKFGKT
AGNAIWLDASKTSPYEFYQFWFNTDDRDVIRFIRYFTFLSVEEITELEKEVETQPEKRVAQKRLAEEMTKTVHDEAALEQ
AKRITNALFSGDIKQLNVEEVEQAFKDVPNHIITREPVGLIDLLVDAKISSSKRQAREDVKNGAIYINGERVQDMNYHVE
EKDHLGNKFTVVRRGKKKYFLIQVESIS

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=404, Percent_Identity=37.6237623762376, Blast_Score=259, Evalue=3e-69,
Organism=Escherichia coli, GI1787925, Length=424, Percent_Identity=52.122641509434, Blast_Score=459, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI25145802, Length=433, Percent_Identity=34.8729792147806, Blast_Score=242, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI25145805, Length=333, Percent_Identity=32.1321321321321, Blast_Score=171, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6325160, Length=439, Percent_Identity=34.1685649202733, Blast_Score=281, Evalue=1e-76,
Organism=Drosophila melanogaster, GI20130383, Length=439, Percent_Identity=38.7243735763098, Blast_Score=333, Evalue=2e-91,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_OCEIH (Q8CXA5)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693139.1
- ProteinModelPortal:   Q8CXA5
- SMR:   Q8CXA5
- GeneID:   1017584
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2218
- NMPDR:   fig|221109.1.peg.2216
- HOGENOM:   HBG288125
- OMA:   TFYIGFD
- BioCyc:   OIHE221109:OB2218-MONOMER
- BRENDA:   6.1.1.1
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 48663; Mature: 48663

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 34-34 BINDING 171-171 BINDING 175-175 BINDING 239-239

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDILQDLESRGLIHQTTDRERLEKHLQNNQVTLYCGFDPTADSLHIGHLVPLTMLKRFQQ
CCHHHHHHHCCCEECCCCHHHHHHHHCCCCEEEEECCCCCCCCEECHHHHHHHHHHHHHH
AGHKPIALVGGGTGMIGDPSGRSSERSLNAPEVVHGFTSKLQQQIAKLVDMESNASENTV
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
VARNNHDWLGNMTIIDFLRDAGKHFGINYMLAKESVSARIEQGITFTEFSYMILQSLDYL
EEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHH
KLYEEENCTLQIGGSDQWGNITAGMELIRRSRENLDENIEVFGLTVPLITKADGTKFGKT
HHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCCCC
AGNAIWLDASKTSPYEFYQFWFNTDDRDVIRFIRYFTFLSVEEITELEKEVETQPEKRVA
CCCEEEEECCCCCCHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
QKRLAEEMTKTVHDEAALEQAKRITNALFSGDIKQLNVEEVEQAFKDVPNHIITREPVGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHCCCCCCHH
IDLLVDAKISSSKRQAREDVKNGAIYINGERVQDMNYHVEEKDHLGNKFTVVRRGKKKYF
HHHHHHCCCCHHHHHHHHHHHCCEEEECCCEEECCCCCCCHHHHCCCEEEEEECCCCEEE
LIQVESIS
EEEEECCC
>Mature Secondary Structure
MDILQDLESRGLIHQTTDRERLEKHLQNNQVTLYCGFDPTADSLHIGHLVPLTMLKRFQQ
CCHHHHHHHCCCEECCCCHHHHHHHHCCCCEEEEECCCCCCCCEECHHHHHHHHHHHHHH
AGHKPIALVGGGTGMIGDPSGRSSERSLNAPEVVHGFTSKLQQQIAKLVDMESNASENTV
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
VARNNHDWLGNMTIIDFLRDAGKHFGINYMLAKESVSARIEQGITFTEFSYMILQSLDYL
EEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHH
KLYEEENCTLQIGGSDQWGNITAGMELIRRSRENLDENIEVFGLTVPLITKADGTKFGKT
HHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCCCC
AGNAIWLDASKTSPYEFYQFWFNTDDRDVIRFIRYFTFLSVEEITELEKEVETQPEKRVA
CCCEEEEECCCCCCHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
QKRLAEEMTKTVHDEAALEQAKRITNALFSGDIKQLNVEEVEQAFKDVPNHIITREPVGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHCCCCCCHH
IDLLVDAKISSSKRQAREDVKNGAIYINGERVQDMNYHVEEKDHLGNKFTVVRRGKKKYF
HHHHHHCCCCHHHHHHHHHHHCCEEEECCCEEECCCCCCCHHHHCCCEEEEEECCCCEEE
LIQVESIS
EEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376