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The map label for this gene is ald [H]

Identifier: 23099643

GI number: 23099643

Start: 2223614

End: 2224729

Strand: Reverse

Name: ald [H]

Synonym: OB2188

Alternate gene names: 23099643

Gene position: 2224729-2223614 (Counterclockwise)

Preceding gene: 23099644

Following gene: 23099641

Centisome position: 61.28

GC content: 37.81

Gene sequence:

>1116_bases
ATGAAAATTGGTATTCCTAAAGAGATTATGAATAATGAAAATCGAGTGGCGATTGCCCCTTCAGGTGTTATGTCATTAAA
AGATGCAGGACATGAGATTTATGTGCAGACTGAAGCTGGACTTGGTTCGAATTTTACTGATGAACAATATATACAAGCTG
GAGCAATAATTGTTTCGACGGCACAAGAAGCATGGTCAAAAGAAATGATTATGAAAGTAAAAGAGCCTCTACAGGAAGAA
TTTATGTATTTTAAAGAAGGACAAATTTTATTTACGTATCTTCATCTGGCTAATGAACCAGAGGTAACGAAAGCTTTAAT
TGAGAAAAAAGTAATTGGAATTGCATATGAAACAGTACAACTGCAAAATCGATCCCTACCATTGCTAACACCGATGAGTG
AGGTAGCAGGGCGTATGGCTACACAAGTAGGTGCACAATTCTTGGAAAAACCTAAGGGCGGCAAAGGCATTATTCTTTCG
AGCATACCAGGTGTGAAAAGAGGAAAAGTAACGATCATAGGTGGAGGGGTAGTTGGAACAAATGCTGCGAAAATAGCAAT
TGGATTAGGAGCTGATGTAACAATTATTGATCTGAATCCGGAGAGGCTACGTCAATTAGATGATATATTTGGTAGCTCCA
TTAATACACTTATGTCTAACTCACTAAATATAGGAGAAGCAGTAAAAGAGTCTGATTTGGTTATAGGTTCTGTACTAATC
CCTGGTGCAAAAGCTCCTAAGTTAGTAAGCGAAAAAATGGTAGAGAGTATGACTGAAGGATCAGTAATAGTGGATGTCGC
TATTGATCAAGGTGGGAATTTTGAGACAAGTGATCGAATTACTTCCCATGATGCTCCAACTTATACAAAGCATGGAGTTC
TCCATTATACAGTTGCAAACATGCCGGGAGCTGTCCCTCGTACATCTACAATTGGGTTAACAAATGTTACAGTTCCTTAT
GCTTTACAATTGGCAAATAAAGGTTACAAACGAGCTTGCCTAGAAAATGAAGCTTTGTTAAGAGGTATTAACACATTAAA
CGGATTTGTAACTTATAAAGCTGTAGCAGATGCCCATCAATTGGAGTATCGTGATGTAATTAGCATTATTGGCTAG

Upstream 100 bases:

>100_bases
TTGCTTGGTGTTAAATTGAAACAATAGTGCGCTTCTTCGGAATATTTGTTACAATTTACTTATCGAAGCTCGTACATCAA
TTTGGAAAAGAGGGTTATAC

Downstream 100 bases:

>100_bases
CAGAAAAAATGCTGTTAAAAAACCGACCTTGCATTTTCGCGAGGCCGGTTTTTTAACGTAACTGTTTAATTCACTATTTG
TAGGTTTTTCGGATATTTAG

Product: alanine dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MKIGIPKEIMNNENRVAIAPSGVMSLKDAGHEIYVQTEAGLGSNFTDEQYIQAGAIIVSTAQEAWSKEMIMKVKEPLQEE
FMYFKEGQILFTYLHLANEPEVTKALIEKKVIGIAYETVQLQNRSLPLLTPMSEVAGRMATQVGAQFLEKPKGGKGIILS
SIPGVKRGKVTIIGGGVVGTNAAKIAIGLGADVTIIDLNPERLRQLDDIFGSSINTLMSNSLNIGEAVKESDLVIGSVLI
PGAKAPKLVSEKMVESMTEGSVIVDVAIDQGGNFETSDRITSHDAPTYTKHGVLHYTVANMPGAVPRTSTIGLTNVTVPY
ALQLANKGYKRACLENEALLRGINTLNGFVTYKAVADAHQLEYRDVISIIG

Sequences:

>Translated_371_residues
MKIGIPKEIMNNENRVAIAPSGVMSLKDAGHEIYVQTEAGLGSNFTDEQYIQAGAIIVSTAQEAWSKEMIMKVKEPLQEE
FMYFKEGQILFTYLHLANEPEVTKALIEKKVIGIAYETVQLQNRSLPLLTPMSEVAGRMATQVGAQFLEKPKGGKGIILS
SIPGVKRGKVTIIGGGVVGTNAAKIAIGLGADVTIIDLNPERLRQLDDIFGSSINTLMSNSLNIGEAVKESDLVIGSVLI
PGAKAPKLVSEKMVESMTEGSVIVDVAIDQGGNFETSDRITSHDAPTYTKHGVLHYTVANMPGAVPRTSTIGLTNVTVPY
ALQLANKGYKRACLENEALLRGINTLNGFVTYKAVADAHQLEYRDVISIIG
>Mature_371_residues
MKIGIPKEIMNNENRVAIAPSGVMSLKDAGHEIYVQTEAGLGSNFTDEQYIQAGAIIVSTAQEAWSKEMIMKVKEPLQEE
FMYFKEGQILFTYLHLANEPEVTKALIEKKVIGIAYETVQLQNRSLPLLTPMSEVAGRMATQVGAQFLEKPKGGKGIILS
SIPGVKRGKVTIIGGGVVGTNAAKIAIGLGADVTIIDLNPERLRQLDDIFGSSINTLMSNSLNIGEAVKESDLVIGSVLI
PGAKAPKLVSEKMVESMTEGSVIVDVAIDQGGNFETSDRITSHDAPTYTKHGVLHYTVANMPGAVPRTSTIGLTNVTVPY
ALQLANKGYKRACLENEALLRGINTLNGFVTYKAVADAHQLEYRDVISIIG

Specific function: This enzyme is a key factor in the assimilation of L- alanine as an energy source through the tricarboxylic acid cycle during sporulation [H]

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=346, Percent_Identity=29.4797687861272, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI122939153, Length=346, Percent_Identity=29.4797687861272, Blast_Score=140, Evalue=3e-33,
Organism=Escherichia coli, GI1787887, Length=378, Percent_Identity=31.4814814814815, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17550456, Length=346, Percent_Identity=28.6127167630058, Blast_Score=137, Evalue=7e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.4.1.1 [H]

Molecular weight: Translated: 39914; Mature: 39914

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGIPKEIMNNENRVAIAPSGVMSLKDAGHEIYVQTEAGLGSNFTDEQYIQAGAIIVST
CCCCCCHHHHCCCCEEEECCCCCHHHHCCCCEEEEEECCCCCCCCCCHHHHHCCEEEEEC
AQEAWSKEMIMKVKEPLQEEFMYFKEGQILFTYLHLANEPEVTKALIEKKVIGIAYETVQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHEEEEEEE
LQNRSLPLLTPMSEVAGRMATQVGAQFLEKPKGGKGIILSSIPGVKRGKVTIIGGGVVGT
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCEECC
NAAKIAIGLGADVTIIDLNPERLRQLDDIFGSSINTLMSNSLNIGEAVKESDLVIGSVLI
CCEEEEEECCCCEEEEECCHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHCCEEEEEEEC
PGAKAPKLVSEKMVESMTEGSVIVDVAIDQGGNFETSDRITSHDAPTYTKHGVLHYTVAN
CCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEECC
MPGAVPRTSTIGLTNVTVPYALQLANKGYKRACLENEALLRGINTLNGFVTYKAVADAHQ
CCCCCCCCCCCEEEECCHHHHHHHHCCCHHHHHHCCHHHHHHHHHHCCEEEEEEHHHHHH
LEYRDVISIIG
HHHHHHHHHCC
>Mature Secondary Structure
MKIGIPKEIMNNENRVAIAPSGVMSLKDAGHEIYVQTEAGLGSNFTDEQYIQAGAIIVST
CCCCCCHHHHCCCCEEEECCCCCHHHHCCCCEEEEEECCCCCCCCCCHHHHHCCEEEEEC
AQEAWSKEMIMKVKEPLQEEFMYFKEGQILFTYLHLANEPEVTKALIEKKVIGIAYETVQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHEEEEEEE
LQNRSLPLLTPMSEVAGRMATQVGAQFLEKPKGGKGIILSSIPGVKRGKVTIIGGGVVGT
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCEECC
NAAKIAIGLGADVTIIDLNPERLRQLDDIFGSSINTLMSNSLNIGEAVKESDLVIGSVLI
CCEEEEEECCCCEEEEECCHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHCCEEEEEEEC
PGAKAPKLVSEKMVESMTEGSVIVDVAIDQGGNFETSDRITSHDAPTYTKHGVLHYTVAN
CCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEECC
MPGAVPRTSTIGLTNVTVPYALQLANKGYKRACLENEALLRGINTLNGFVTYKAVADAHQ
CCCCCCCCCCCEEEECCHHHHHHHHCCCHHHHHHCCHHHHHHHHHHCCEEEEEEHHHHHH
LEYRDVISIIG
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2340274 [H]