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The map label for this gene is ytsJ [H]

Identifier: 23099631

GI number: 23099631

Start: 2211188

End: 2212423

Strand: Reverse

Name: ytsJ [H]

Synonym: OB2176

Alternate gene names: 23099631

Gene position: 2212423-2211188 (Counterclockwise)

Preceding gene: 23099632

Following gene: 23099630

Centisome position: 60.94

GC content: 37.94

Gene sequence:

>1236_bases
ATGGCAAACTTAAGAGATGAAGCTTTGCATTTACATAAAGTAAAGCAAGGTAAATTAGAAATAAATACAAAGGTTCCCGT
AAAAGATGCAAAGGATTTAAGTTTAGCTTATTCTCCTGGTGTAGCAGAACCATGTAAAGAAATTTATGACCGTAAAGAAA
CAGTATATGACTATACGATGAAAGGTAACATGGTTGCGGTAGTGAGTGACGGATCAGCGGTATTGGGATTAGGGAATATC
GGACCTGAAGCTGCACTACCGGTTATGGAAGGGAAAGCAGTATTATTTAAAGGTTTTGCTGGAGTAGATGCTTTTCCAAT
TTGTGTAGATACACACGATGTAGAGCAAATAGTTCAAACTGTTAAATTACTAGAACCGAATTTCGGGGGAGTAAATTTAG
AAGATATTGCAGCACCAAACTGTTTTATTATTGAAGATAGATTAAAGAGTGAGACAAATATTCCTATTTTTCACGATGAT
CAACATGGAACAGCTATTGTTACAGTAGCTGGATTAATTAATGCATTAAAACTAGTAGGAAAGAAATTCTCTGATATTAA
AGTTGTAGCGAATGGAGCAGGTGCAGCTGGTATTGCAATTATTCGTCTATTACAAAGCTTCGGTGTACGAGATATGATCA
TGTGCGACTCTAAAGGTGCAATATATGAAGGACGGCCATACGGTATGAATACTGTAAAAGACAGTGTTGCTAAGTATACC
AACGCCAATAAGGTTGAAGGTAGTCTTTCAGATGTGCTAGAAGGTGCAGATGTATTTATTGGTGTTTCTGTTGGCGGTGC
TTTAACAAAAGATATGGTAAGAAGCATGAATGATGACGCGATAATTTTTGCAATGGCAAATCCAGATCCAGAAATCCTTC
CAGAGGATGCGAAAGAAGCAGGAGCTAGAGTGATTGGAACAGGACGATCTGATTTTGCCAATCAAGTTAATAATGTTCTA
GCTTTTCCTGGTATTTTCCGCGGAGCATTGGATGTTAGAGCGACTAGAATAAATGAAGAAATGAAAGTAGCAGCCGCTGA
AGGGATAGCTACGCTTATTTCCGATGATGAATTACACGATGATTATGTTATTCCGTCACCGTTTGATGAAAGAGTAGCAC
CATTAGTAGCTTCTCATGTTGCAAAAGCAGCAATGAGTACTGGAGTCGCTAGAATGAATGTAGATCCTGAAGAAGTAGCA
GAAAAGACAAGGAAATTAACACAGCAGGAAAAATAA

Upstream 100 bases:

>100_bases
GTTATAATATATAGAGGTCACAAGTGGTCTGACCACTTGCGGTATCGCTATTTTAAAATATGTATTATCGATAACGAAGT
TTAAAAGGAGAGGTACTCTT

Downstream 100 bases:

>100_bases
GGAGTGAATCCCCGCATGACAGTAACAAATAAACAAAAGGTTTATCAAAGCGTAATTAAAGAGTTACAGCGCTATATAGA
GATAAACCATTTAAATGCCG

Product: malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME 4 [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MANLRDEALHLHKVKQGKLEINTKVPVKDAKDLSLAYSPGVAEPCKEIYDRKETVYDYTMKGNMVAVVSDGSAVLGLGNI
GPEAALPVMEGKAVLFKGFAGVDAFPICVDTHDVEQIVQTVKLLEPNFGGVNLEDIAAPNCFIIEDRLKSETNIPIFHDD
QHGTAIVTVAGLINALKLVGKKFSDIKVVANGAGAAGIAIIRLLQSFGVRDMIMCDSKGAIYEGRPYGMNTVKDSVAKYT
NANKVEGSLSDVLEGADVFIGVSVGGALTKDMVRSMNDDAIIFAMANPDPEILPEDAKEAGARVIGTGRSDFANQVNNVL
AFPGIFRGALDVRATRINEEMKVAAAEGIATLISDDELHDDYVIPSPFDERVAPLVASHVAKAAMSTGVARMNVDPEEVA
EKTRKLTQQEK

Sequences:

>Translated_411_residues
MANLRDEALHLHKVKQGKLEINTKVPVKDAKDLSLAYSPGVAEPCKEIYDRKETVYDYTMKGNMVAVVSDGSAVLGLGNI
GPEAALPVMEGKAVLFKGFAGVDAFPICVDTHDVEQIVQTVKLLEPNFGGVNLEDIAAPNCFIIEDRLKSETNIPIFHDD
QHGTAIVTVAGLINALKLVGKKFSDIKVVANGAGAAGIAIIRLLQSFGVRDMIMCDSKGAIYEGRPYGMNTVKDSVAKYT
NANKVEGSLSDVLEGADVFIGVSVGGALTKDMVRSMNDDAIIFAMANPDPEILPEDAKEAGARVIGTGRSDFANQVNNVL
AFPGIFRGALDVRATRINEEMKVAAAEGIATLISDDELHDDYVIPSPFDERVAPLVASHVAKAAMSTGVARMNVDPEEVA
EKTRKLTQQEK
>Mature_410_residues
ANLRDEALHLHKVKQGKLEINTKVPVKDAKDLSLAYSPGVAEPCKEIYDRKETVYDYTMKGNMVAVVSDGSAVLGLGNIG
PEAALPVMEGKAVLFKGFAGVDAFPICVDTHDVEQIVQTVKLLEPNFGGVNLEDIAAPNCFIIEDRLKSETNIPIFHDDQ
HGTAIVTVAGLINALKLVGKKFSDIKVVANGAGAAGIAIIRLLQSFGVRDMIMCDSKGAIYEGRPYGMNTVKDSVAKYTN
ANKVEGSLSDVLEGADVFIGVSVGGALTKDMVRSMNDDAIIFAMANPDPEILPEDAKEAGARVIGTGRSDFANQVNNVLA
FPGIFRGALDVRATRINEEMKVAAAEGIATLISDDELHDDYVIPSPFDERVAPLVASHVAKAAMSTGVARMNVDPEEVAE
KTRKLTQQEK

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=396, Percent_Identity=25.2525252525253, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI62420882, Length=416, Percent_Identity=26.9230769230769, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI62420880, Length=416, Percent_Identity=26.9230769230769, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI239049447, Length=416, Percent_Identity=26.9230769230769, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI4505145, Length=415, Percent_Identity=23.6144578313253, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI1788806, Length=406, Percent_Identity=50.4926108374384, Blast_Score=386, Evalue=1e-108,
Organism=Escherichia coli, GI87081919, Length=448, Percent_Identity=28.3482142857143, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17537199, Length=434, Percent_Identity=25.1152073732719, Blast_Score=78, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6322823, Length=349, Percent_Identity=27.7936962750716, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI78707242, Length=476, Percent_Identity=24.3697478991597, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI78707238, Length=476, Percent_Identity=24.3697478991597, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI281362674, Length=459, Percent_Identity=26.5795206971678, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI281362672, Length=459, Percent_Identity=26.5795206971678, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21356279, Length=459, Percent_Identity=26.5795206971678, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24646388, Length=393, Percent_Identity=27.4809160305344, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24646386, Length=393, Percent_Identity=27.4809160305344, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 43969; Mature: 43838

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANLRDEALHLHKVKQGKLEINTKVPVKDAKDLSLAYSPGVAEPCKEIYDRKETVYDYTM
CCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEECCCCHHHHHHHHCCHHHEEEEEE
KGNMVAVVSDGSAVLGLGNIGPEAALPVMEGKAVLFKGFAGVDAFPICVDTHDVEQIVQT
CCCEEEEEECCCEEEEECCCCCHHCCCHHCCCEEEEECCCCCCCCEEEECCHHHHHHHHH
VKLLEPNFGGVNLEDIAAPNCFIIEDRLKSETNIPIFHDDQHGTAIVTVAGLINALKLVG
HHHHCCCCCCCCHHHHCCCCEEEEEHHHCCCCCCCEEECCCCCEEEEEHHHHHHHHHHHC
KKFSDIKVVANGAGAAGIAIIRLLQSFGVRDMIMCDSKGAIYEGRPYGMNTVKDSVAKYT
CCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCEECCCCCCCHHHHHHHHHHC
NANKVEGSLSDVLEGADVFIGVSVGGALTKDMVRSMNDDAIIFAMANPDPEILPEDAKEA
CCCCCCCCHHHHHCCCCEEEEEECCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHC
GARVIGTGRSDFANQVNNVLAFPGIFRGALDVRATRINEEMKVAAAEGIATLISDDELHD
CCEEEECCCHHHHHHHHHEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCC
DYVIPSPFDERVAPLVASHVAKAAMSTGVARMNVDPEEVAEKTRKLTQQEK
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ANLRDEALHLHKVKQGKLEINTKVPVKDAKDLSLAYSPGVAEPCKEIYDRKETVYDYTM
CCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEECCCCHHHHHHHHCCHHHEEEEEE
KGNMVAVVSDGSAVLGLGNIGPEAALPVMEGKAVLFKGFAGVDAFPICVDTHDVEQIVQT
CCCEEEEEECCCEEEEECCCCCHHCCCHHCCCEEEEECCCCCCCCEEEECCHHHHHHHHH
VKLLEPNFGGVNLEDIAAPNCFIIEDRLKSETNIPIFHDDQHGTAIVTVAGLINALKLVG
HHHHCCCCCCCCHHHHCCCCEEEEEHHHCCCCCCCEEECCCCCEEEEEHHHHHHHHHHHC
KKFSDIKVVANGAGAAGIAIIRLLQSFGVRDMIMCDSKGAIYEGRPYGMNTVKDSVAKYT
CCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCEECCCCCCCHHHHHHHHHHC
NANKVEGSLSDVLEGADVFIGVSVGGALTKDMVRSMNDDAIIFAMANPDPEILPEDAKEA
CCCCCCCCHHHHHCCCCEEEEEECCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHC
GARVIGTGRSDFANQVNNVLAFPGIFRGALDVRATRINEEMKVAAAEGIATLISDDELHD
CCEEEECCCHHHHHHHHHEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCC
DYVIPSPFDERVAPLVASHVAKAAMSTGVARMNVDPEEVAEKTRKLTQQEK
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]