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The map label for this gene is thrS

Identifier: 23099609

GI number: 23099609

Start: 2185413

End: 2187353

Strand: Reverse

Name: thrS

Synonym: OB2154

Alternate gene names: 23099609

Gene position: 2187353-2185413 (Counterclockwise)

Preceding gene: 23099610

Following gene: 23099608

Centisome position: 60.25

GC content: 34.88

Gene sequence:

>1941_bases
ATGGCAGATTTATCGATTATTTTCCCAGATGGGTCAGAGAAGCAATTTCCAGTCGGAACTACGGGTGAAGAAATAGCAGC
ATCAATATCACCAGGTTTAAAAAAACAAGCACTTGCTATCAAACTTGATGGTGAACTTGTAGATTTACGAAGAGAATTAT
CATCTGGTGGAAGCATTGAAATCATCACATATAAAAATAATGAGGGCCTTGAAGTGTTACGTCATTCAAGTGCACATTTG
TTAGCTCAAGCTATAAAACGTTTGTTTAACGATGTGAAACTTGGTGTCGGTCCTGTCATAGAAGAAGGATTTTATTATGA
TATTGATATGGAGCACAAATTAACTCCAGAAGATTTACCTAAAATTGAAAAAGAAATGAAGCGTATTATTGATGAAAATT
TAGAAATCAAAAGGGTAGAAGTTTCTCGTAATAAAGCTCAAGAAATGTTTAGCGATATTGGCGATGATTTAAAATTAGAA
CTTATCGACGCTATTCCGGAAAATGAACAAGTAACGATTTATGAACAAGGAGAGTTTTTCGATTTATGCCGAGGGATTCA
TGTCCCTTCTACAAGTAAAATTAAAGCATTTAAATTATTAAGTATTTCTGGAGCTTATTGGCGTGGAGATAGTAACAATA
AACAATTACAACGAATTTATGGCACAGCTTTTGAGAAAAAAGGACAATTAGAAGAACATTTACAAATACTAGAAGAAAGA
AAAGAAAGAGATCATCGTAAATTAGGAAAAGAATTAGGTATATTTACAGTATCACAAAAAGTTGGGCAAGGCTTACCGCT
TTGGTTACCAAAAGGAGCAACGATTCGTCGTAATGTAGAACGCTATATTGTTGATTTAGAAGAAAGATTAGGATATAGCC
ATGTTTATACTCCGGTCCTGGGAAATGTAGAACTGTATAAAACAAGTGGACATTGGGATCACTATCAGGACGATATGTTT
CCAACAATGGAGATGGATAACGAAGAACTGGTACTTCGTCCAATGAATTGTCCTCATCATATGATGGTATTTAAAAATCA
ACTCTGGAGTTATCGTAATCTGCCAGTTCGAATCGCTGAGCTAGGAACAATGCATCGACATGAAATGAGTGGTGCGTTAG
CTGGCTTACAACGTGTACGAGCAATGACATTAAATGATGCACATATTTTTGCTCGTCCTGATCAGTTAAAAGAAGAATTT
ATTCGTGTAGTTGAATTAGTACAACATGTATATAAAGATTTTGGTATAGACGATTACTATTTCCGATTATCTTATCGTGA
TCCGGAGGATAAAGAAAAGTATGTTGATAATGACGAGATGTGGGAAAAAGCACAAGCGATGTTAAAAGAAACAATGGAAG
ATATGAATTTAGAATATGTAGAAGCAGAAGGTGAAGCTGCATTTTATGGTCCAAAATTAGATGTACAAGTCAAAACTGCT
CTAGGCAAAGACGAAACCCTATCGACAATCCAATTAGATTTCCATTTACCAGAGCGATTTGATCTAACATATATTGGAGA
AGATGGTAATCAACATCGCCCAGTAGTTATCCATCGCGGTGTAGTATCAACAATGGAACGATTTGTTGCTTTCTTAATTG
AAGAATATAAAGGTGCTTTTCCAACTTGGCTTGCACCGGTACAAGTAAAAATTATCCCAGTTTCATTACAAGCTCATTTG
GATTATGCGAAGGATATTGAAGAAAAGCTTCGATACCAAGGAGTTCGTGTAGAACTTGATGAACGAGATGAGAAAATTGG
ATATAAAATTCGAGAAGCACAGACACAAAAAGTTCCATTTGCTCTAGTATTAGGAGACAAAGAAATGGAAAGTAACAGTG
TTAATTATCGTAGATACGGAGAACAAGATACCGAGACATTGGAATTTGATCAATTCTTAAATCTTATTAAAGAAGAAGTA
GATAATAAGAAGATTAAATAA

Upstream 100 bases:

>100_bases
TAGGAATTAGGGTGGAACCACGACGTTACCAACGCTCGTCCCTTTGCTATAGTGCAAAGGGATGGGCGTTTTTTAATTAT
TTGAAAAAGGAGAATGAAAC

Downstream 100 bases:

>100_bases
GCGTTATTGATAAGGAATATTATAAAAACCGAACTGTAACAAAATGTTATTCATTTTCGCTACAGTTCGGTTTTTTGTGA
GCTCAGGTTGGGCTTTATCA

Product: threonine-tRNA ligase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 646; Mature: 645

Protein sequence:

>646_residues
MADLSIIFPDGSEKQFPVGTTGEEIAASISPGLKKQALAIKLDGELVDLRRELSSGGSIEIITYKNNEGLEVLRHSSAHL
LAQAIKRLFNDVKLGVGPVIEEGFYYDIDMEHKLTPEDLPKIEKEMKRIIDENLEIKRVEVSRNKAQEMFSDIGDDLKLE
LIDAIPENEQVTIYEQGEFFDLCRGIHVPSTSKIKAFKLLSISGAYWRGDSNNKQLQRIYGTAFEKKGQLEEHLQILEER
KERDHRKLGKELGIFTVSQKVGQGLPLWLPKGATIRRNVERYIVDLEERLGYSHVYTPVLGNVELYKTSGHWDHYQDDMF
PTMEMDNEELVLRPMNCPHHMMVFKNQLWSYRNLPVRIAELGTMHRHEMSGALAGLQRVRAMTLNDAHIFARPDQLKEEF
IRVVELVQHVYKDFGIDDYYFRLSYRDPEDKEKYVDNDEMWEKAQAMLKETMEDMNLEYVEAEGEAAFYGPKLDVQVKTA
LGKDETLSTIQLDFHLPERFDLTYIGEDGNQHRPVVIHRGVVSTMERFVAFLIEEYKGAFPTWLAPVQVKIIPVSLQAHL
DYAKDIEEKLRYQGVRVELDERDEKIGYKIREAQTQKVPFALVLGDKEMESNSVNYRRYGEQDTETLEFDQFLNLIKEEV
DNKKIK

Sequences:

>Translated_646_residues
MADLSIIFPDGSEKQFPVGTTGEEIAASISPGLKKQALAIKLDGELVDLRRELSSGGSIEIITYKNNEGLEVLRHSSAHL
LAQAIKRLFNDVKLGVGPVIEEGFYYDIDMEHKLTPEDLPKIEKEMKRIIDENLEIKRVEVSRNKAQEMFSDIGDDLKLE
LIDAIPENEQVTIYEQGEFFDLCRGIHVPSTSKIKAFKLLSISGAYWRGDSNNKQLQRIYGTAFEKKGQLEEHLQILEER
KERDHRKLGKELGIFTVSQKVGQGLPLWLPKGATIRRNVERYIVDLEERLGYSHVYTPVLGNVELYKTSGHWDHYQDDMF
PTMEMDNEELVLRPMNCPHHMMVFKNQLWSYRNLPVRIAELGTMHRHEMSGALAGLQRVRAMTLNDAHIFARPDQLKEEF
IRVVELVQHVYKDFGIDDYYFRLSYRDPEDKEKYVDNDEMWEKAQAMLKETMEDMNLEYVEAEGEAAFYGPKLDVQVKTA
LGKDETLSTIQLDFHLPERFDLTYIGEDGNQHRPVVIHRGVVSTMERFVAFLIEEYKGAFPTWLAPVQVKIIPVSLQAHL
DYAKDIEEKLRYQGVRVELDERDEKIGYKIREAQTQKVPFALVLGDKEMESNSVNYRRYGEQDTETLEFDQFLNLIKEEV
DNKKIK
>Mature_645_residues
ADLSIIFPDGSEKQFPVGTTGEEIAASISPGLKKQALAIKLDGELVDLRRELSSGGSIEIITYKNNEGLEVLRHSSAHLL
AQAIKRLFNDVKLGVGPVIEEGFYYDIDMEHKLTPEDLPKIEKEMKRIIDENLEIKRVEVSRNKAQEMFSDIGDDLKLEL
IDAIPENEQVTIYEQGEFFDLCRGIHVPSTSKIKAFKLLSISGAYWRGDSNNKQLQRIYGTAFEKKGQLEEHLQILEERK
ERDHRKLGKELGIFTVSQKVGQGLPLWLPKGATIRRNVERYIVDLEERLGYSHVYTPVLGNVELYKTSGHWDHYQDDMFP
TMEMDNEELVLRPMNCPHHMMVFKNQLWSYRNLPVRIAELGTMHRHEMSGALAGLQRVRAMTLNDAHIFARPDQLKEEFI
RVVELVQHVYKDFGIDDYYFRLSYRDPEDKEKYVDNDEMWEKAQAMLKETMEDMNLEYVEAEGEAAFYGPKLDVQVKTAL
GKDETLSTIQLDFHLPERFDLTYIGEDGNQHRPVVIHRGVVSTMERFVAFLIEEYKGAFPTWLAPVQVKIIPVSLQAHLD
YAKDIEEKLRYQGVRVELDERDEKIGYKIREAQTQKVPFALVLGDKEMESNSVNYRRYGEQDTETLEFDQFLNLIKEEVD
NKKIK

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38570111, Length=618, Percent_Identity=37.378640776699, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI38202255, Length=635, Percent_Identity=36.0629921259843, Blast_Score=414, Evalue=1e-115,
Organism=Homo sapiens, GI20070344, Length=628, Percent_Identity=35.1910828025478, Blast_Score=366, Evalue=1e-101,
Organism=Escherichia coli, GI1788013, Length=642, Percent_Identity=41.1214953271028, Blast_Score=536, Evalue=1e-153,
Organism=Escherichia coli, GI1786392, Length=160, Percent_Identity=25, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71984184, Length=618, Percent_Identity=39.1585760517799, Blast_Score=449, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI71984192, Length=618, Percent_Identity=39.1585760517799, Blast_Score=449, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6322112, Length=634, Percent_Identity=35.4889589905363, Blast_Score=406, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6322654, Length=404, Percent_Identity=34.6534653465347, Blast_Score=249, Evalue=8e-67,
Organism=Drosophila melanogaster, GI24583839, Length=617, Percent_Identity=37.2771474878444, Blast_Score=441, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24583842, Length=617, Percent_Identity=37.2771474878444, Blast_Score=441, Evalue=1e-123,
Organism=Drosophila melanogaster, GI28574141, Length=617, Percent_Identity=37.2771474878444, Blast_Score=441, Evalue=1e-123,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_OCEIH (Q8EPF3)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693075.1
- ProteinModelPortal:   Q8EPF3
- SMR:   Q8EPF3
- GeneID:   1015610
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2154
- NMPDR:   fig|221109.1.peg.2152
- HOGENOM:   HBG352811
- OMA:   GRKWQLG
- ProtClustDB:   CLSK2755913
- BioCyc:   OIHE221109:OB2154-MONOMER
- BRENDA:   6.1.1.3
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF81271 TGS-like; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 74787; Mature: 74656

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50862 AA_TRNA_LIGASE_II; PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLSIIFPDGSEKQFPVGTTGEEIAASISPGLKKQALAIKLDGELVDLRRELSSGGSIE
CCCEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHCCCCCEE
IITYKNNEGLEVLRHSSAHLLAQAIKRLFNDVKLGVGPVIEEGFYYDIDMEHKLTPEDLP
EEEEECCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHCCEEEEECCCCCCCHHHHH
KIEKEMKRIIDENLEIKRVEVSRNKAQEMFSDIGDDLKLELIDAIPENEQVTIYEQGEFF
HHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCHH
DLCRGIHVPSTSKIKAFKLLSISGAYWRGDSNNKQLQRIYGTAFEKKGQLEEHLQILEER
HHHCCCCCCCCCCEEEEEEEEECCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
KERDHRKLGKELGIFTVSQKVGQGLPLWLPKGATIRRNVERYIVDLEERLGYSHVYTPVL
HHHHHHHHHHHHCEEEEEHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCEECCCCC
GNVELYKTSGHWDHYQDDMFPTMEMDNEELVLRPMNCPHHMMVFKNQLWSYRNLPVRIAE
CCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEHH
LGTMHRHEMSGALAGLQRVRAMTLNDAHIFARPDQLKEEFIRVVELVQHVYKDFGIDDYY
HCCHHHHHHHHHHHHHHHHHHHEECCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEE
FRLSYRDPEDKEKYVDNDEMWEKAQAMLKETMEDMNLEYVEAEGEAAFYGPKLDVQVKTA
EEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEECCEEEEEEEEC
LGKDETLSTIQLDFHLPERFDLTYIGEDGNQHRPVVIHRGVVSTMERFVAFLIEEYKGAF
CCCCCCEEEEEEEEECCCCCCEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCC
PTWLAPVQVKIIPVSLQAHLDYAKDIEEKLRYQGVRVELDERDEKIGYKIREAQTQKVPF
CCEECCEEEEEEEEEHHHHCCHHHHHHHHHHHCCCEEEECCCCCHHCEEEHHCCCCCCCE
ALVLGDKEMESNSVNYRRYGEQDTETLEFDQFLNLIKEEVDNKKIK
EEEECCCCCCCCCCCHHCCCCCCCHHCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ADLSIIFPDGSEKQFPVGTTGEEIAASISPGLKKQALAIKLDGELVDLRRELSSGGSIE
CCEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHCCCCCEE
IITYKNNEGLEVLRHSSAHLLAQAIKRLFNDVKLGVGPVIEEGFYYDIDMEHKLTPEDLP
EEEEECCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHCCEEEEECCCCCCCHHHHH
KIEKEMKRIIDENLEIKRVEVSRNKAQEMFSDIGDDLKLELIDAIPENEQVTIYEQGEFF
HHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCHH
DLCRGIHVPSTSKIKAFKLLSISGAYWRGDSNNKQLQRIYGTAFEKKGQLEEHLQILEER
HHHCCCCCCCCCCEEEEEEEEECCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
KERDHRKLGKELGIFTVSQKVGQGLPLWLPKGATIRRNVERYIVDLEERLGYSHVYTPVL
HHHHHHHHHHHHCEEEEEHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCEECCCCC
GNVELYKTSGHWDHYQDDMFPTMEMDNEELVLRPMNCPHHMMVFKNQLWSYRNLPVRIAE
CCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEHH
LGTMHRHEMSGALAGLQRVRAMTLNDAHIFARPDQLKEEFIRVVELVQHVYKDFGIDDYY
HCCHHHHHHHHHHHHHHHHHHHEECCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEE
FRLSYRDPEDKEKYVDNDEMWEKAQAMLKETMEDMNLEYVEAEGEAAFYGPKLDVQVKTA
EEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEECCEEEEEEEEC
LGKDETLSTIQLDFHLPERFDLTYIGEDGNQHRPVVIHRGVVSTMERFVAFLIEEYKGAF
CCCCCCEEEEEEEEECCCCCCEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCC
PTWLAPVQVKIIPVSLQAHLDYAKDIEEKLRYQGVRVELDERDEKIGYKIREAQTQKVPF
CCEECCEEEEEEEEEHHHHCCHHHHHHHHHHHCCCEEEECCCCCHHCEEEHHCCCCCCCE
ALVLGDKEMESNSVNYRRYGEQDTETLEFDQFLNLIKEEVDNKKIK
EEEECCCCCCCCCCCHHCCCCCCCHHCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376