Click here to switch to the map view.

The map label for this gene is infC

Identifier: 23099607

GI number: 23099607

Start: 2182664

End: 2183170

Strand: Reverse

Name: infC

Synonym: OB2152

Alternate gene names: 23099607

Gene position: 2183170-2182664 (Counterclockwise)

Preceding gene: 23099608

Following gene: 23099606

Centisome position: 60.13

GC content: 38.86

Gene sequence:

>507_bases
ATGAACGTTAATGAAAAGATTCGTGCTAGAGAAGTACGACTTATCGACTCAAACGGTGATCAGCTTGGAGTAAAATCTCG
TCAGGAAGCATTAGATATTGCAACAACAAGAAATCTTGACTTAGTACTAGTTGCTCCAAATGCGAAACCGCCAGTATGTC
GTATCATGGACTACGGTAAATATCGTTTCGAGCAACAGAAAAAAGAAAAAGAGGCTCGTAAAAAGCAAAAAGTCATTAAT
GTAAAAGAGGTTCGTTTCACACCAGGAATTGGTGACCATGATTTTGAGACAAAATTGAAAAACGCTCGCAAGTTCCTTGA
AAAAGGGGACAAAGTAAAAGCTGCAGTGCGTTTCCGTGGTCGTGCAATTACCCATAAAGAGCTTGGTCGTGAAGTGTTAG
ATCGTTTCGCTGAGGAAGTAAAAGACCTTGGTACGATTGAAACAAAACCTAAAATGGAAGGCCGTAATATGTTTATGATG
GTCGCTCCAATTAATGAAAAAAATTAA

Upstream 100 bases:

>100_bases
GGGTGCGTGTATGTACCGACACTTTTTTAATTGCATAAAATAAGCTTGTCAAATAGATCAGTAACAATAAACTCGGAGGT
GGCTGGCTATTAGCAAGGAT

Downstream 100 bases:

>100_bases
TACGTAACAGTAGATAACTAGGAGGAATGAATTATGCCTAAAATGAAAACTCATAAAGGTACAGCAAAGCGTTTTAAGAA
AACGGGTTCTGGAAAAGTAA

Product: translation initiation factor IF-3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MNVNEKIRAREVRLIDSNGDQLGVKSRQEALDIATTRNLDLVLVAPNAKPPVCRIMDYGKYRFEQQKKEKEARKKQKVIN
VKEVRFTPGIGDHDFETKLKNARKFLEKGDKVKAAVRFRGRAITHKELGREVLDRFAEEVKDLGTIETKPKMEGRNMFMM
VAPINEKN

Sequences:

>Translated_168_residues
MNVNEKIRAREVRLIDSNGDQLGVKSRQEALDIATTRNLDLVLVAPNAKPPVCRIMDYGKYRFEQQKKEKEARKKQKVIN
VKEVRFTPGIGDHDFETKLKNARKFLEKGDKVKAAVRFRGRAITHKELGREVLDRFAEEVKDLGTIETKPKMEGRNMFMM
VAPINEKN
>Mature_168_residues
MNVNEKIRAREVRLIDSNGDQLGVKSRQEALDIATTRNLDLVLVAPNAKPPVCRIMDYGKYRFEQQKKEKEARKKQKVIN
VKEVRFTPGIGDHDFETKLKNARKFLEKGDKVKAAVRFRGRAITHKELGREVLDRFAEEVKDLGTIETKPKMEGRNMFMM
VAPINEKN

Specific function: IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins

COG id: COG0290

COG function: function code J; Translation initiation factor 3 (IF-3)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-3 family

Homologues:

Organism=Escherichia coli, GI1788012, Length=162, Percent_Identity=50.6172839506173, Blast_Score=157, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): IF3_OCEIH (Q8EPF5)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693073.1
- ProteinModelPortal:   Q8EPF5
- GeneID:   1015611
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2152
- NMPDR:   fig|221109.1.peg.2150
- HOGENOM:   HBG744268
- BioCyc:   OIHE221109:OB2152-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00080
- InterPro:   IPR019813
- InterPro:   IPR001288
- InterPro:   IPR019815
- InterPro:   IPR019814
- Gene3D:   G3DSA:3.10.20.80
- Gene3D:   G3DSA:3.30.110.10
- PANTHER:   PTHR10938
- TIGRFAMs:   TIGR00168

Pfam domain/function: PF00707 IF3_C; PF05198 IF3_N; SSF54364 IF3; SSF55200 IF3

EC number: NA

Molecular weight: Translated: 19396; Mature: 19396

Theoretical pI: Translated: 10.58; Mature: 10.58

Prosite motif: PS00938 IF3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVNEKIRAREVRLIDSNGDQLGVKSRQEALDIATTRNLDLVLVAPNAKPPVCRIMDYGK
CCCCHHHHHEEEEEECCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCEEEEHHCCH
YRFEQQKKEKEARKKQKVINVKEVRFTPGIGDHDFETKLKNARKFLEKGDKVKAAVRFRG
HHHHHHHHHHHHHHHHHCCCHHHEEECCCCCCCHHHHHHHHHHHHHHCCCHHEEHHHHCC
RAITHKELGREVLDRFAEEVKDLGTIETKPKMEGRNMFMMVAPINEKN
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCC
>Mature Secondary Structure
MNVNEKIRAREVRLIDSNGDQLGVKSRQEALDIATTRNLDLVLVAPNAKPPVCRIMDYGK
CCCCHHHHHEEEEEECCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCEEEEHHCCH
YRFEQQKKEKEARKKQKVINVKEVRFTPGIGDHDFETKLKNARKFLEKGDKVKAAVRFRG
HHHHHHHHHHHHHHHHHCCCHHHEEECCCCCCCHHHHHHHHHHHHHHCCCHHEEHHHHCC
RAITHKELGREVLDRFAEEVKDLGTIETKPKMEGRNMFMMVAPINEKN
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376