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The map label for this gene is hemA

Identifier: 23099525

GI number: 23099525

Start: 2097010

End: 2098383

Strand: Reverse

Name: hemA

Synonym: OB2070

Alternate gene names: 23099525

Gene position: 2098383-2097010 (Counterclockwise)

Preceding gene: 23099527

Following gene: 23099524

Centisome position: 57.8

GC content: 33.48

Gene sequence:

>1374_bases
GTGCATATTCTAAAAGTTGGGTTTAATTACAAAACAACCCCTGTTGACATTAGGGAGAAATTTACATTCTCTGAAGATTC
ATTGCAAGATGCTATGGTAGAATTGAAAAATCAAAAAAGCATATTAGAAGATGTAATTATCTCTACTTGCAACCGTACGG
AGATTTACGCGGTTGTAGATCAACTTCATACTGGAAGATATTATATAAAACAGTTTTTATCTAATTGGTTTGGTATTGAA
AAAGAAGAATTTTCTACTTATCTAAGAATAACAGAAGATGACGGAGCAATGGAGCACTTATTTAGAGTATCTACTGGTCT
AGATTCAATGGTGTTAGGAGAAACACAAATTCTTGGTCAAGTGAAACAAGCCTTCTTAAATTCCCAACAAGTTAATACTA
CGGGCACCATATTTAATGAGCTATTTAAACAAGCTATTACTTTTGGGAAGCGTGCACATAAGGAAACTGCTATTGGAGAA
CACGCAGTTTCTATTAGCTATGCAGCAGTGGAATTAGCAAAGAAAATTTTTGGTGATTTGCAAGAGAAACATGTTGCGAT
ACTTGGTGCAGGAAAAATGGGGGAACTAGCAGCAAAAAACATTCAAGGTTCTGGGGCTACAAAAATTACAGTAGTAAATA
GAACGCTTGAAAATGCAAATGAAATGGCAGAGAAATTTAATGCGGATGTTGAGAGTATAGATCAACTTCCAGTTATCTTA
CAGCAGGCTGATATTTTAATTAGCTCTACTGGTGCTGATTCCATTGTTGTTACAAAAGAGATGATGGAGAAAGTACAAAA
ACAGCGAAAAGGTCGCGCGCTTTTTCTTGTTGATATAGCAGTACCACGTGATATGGACCCTGCGATTTCAGAACTTGAAA
ATGTATTTTTGTATGATATTGATAATTTACAGCATATTGTTGATGACAACCTTGAGTCTAGAAAACAAGCCGCTGAAAAA
ATTGAACTATTAATAGAAGAAGAGATTGTTACCTTTAAGGAATGGTTAAAAACATTAGGTGTAATCCCAGTAATATCTGC
ATTAAGACAAAAAGCCTTAACCATTCAGGCAGAAACAATGCAGAGTATTGAACGTAAAATACCAAACCTTACTGACAGAG
AAAGAAAAGTATTAAATAAGCATACAAAAAGTATTATTAATCAATTATTAAAAGAACCTGTTACACAAGCAAAAGAGTTT
GCAGGTAAAGACAATGCAGAAGACTCATTGCAATTATTTATAAATATATTTGGTATTGAAGAAGAGGTTAAGGAAGAATT
AGTTAAACATGCAAAAAAAAATGAAACTTTAATGAAGATTGCTAAAGAAGAACCGTCTTCTTTTCCAATCATTGAAAAAA
TTACAACAGCCTGA

Upstream 100 bases:

>100_bases
TCACATTTTAAAGATTCACGATATAATTTACATGTTATAATAAATATTAGGTTATAATAATATTAAAAAAGTCCGATTTA
AAGATTTTGGGGGTAGTACT

Downstream 100 bases:

>100_bases
TCGAAAGAAAGGATACCGAGATGGTTGAAATAAAATGGCTATACGAATTGTTGTTATTGCTATATAGCTTTAGTCTAATT
GGATTTATGATTGATTTTAT

Product: glutamyl-tRNA reductase

Products: NA

Alternate protein names: GluTR

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MHILKVGFNYKTTPVDIREKFTFSEDSLQDAMVELKNQKSILEDVIISTCNRTEIYAVVDQLHTGRYYIKQFLSNWFGIE
KEEFSTYLRITEDDGAMEHLFRVSTGLDSMVLGETQILGQVKQAFLNSQQVNTTGTIFNELFKQAITFGKRAHKETAIGE
HAVSISYAAVELAKKIFGDLQEKHVAILGAGKMGELAAKNIQGSGATKITVVNRTLENANEMAEKFNADVESIDQLPVIL
QQADILISSTGADSIVVTKEMMEKVQKQRKGRALFLVDIAVPRDMDPAISELENVFLYDIDNLQHIVDDNLESRKQAAEK
IELLIEEEIVTFKEWLKTLGVIPVISALRQKALTIQAETMQSIERKIPNLTDRERKVLNKHTKSIINQLLKEPVTQAKEF
AGKDNAEDSLQLFINIFGIEEEVKEELVKHAKKNETLMKIAKEEPSSFPIIEKITTA

Sequences:

>Translated_457_residues
MHILKVGFNYKTTPVDIREKFTFSEDSLQDAMVELKNQKSILEDVIISTCNRTEIYAVVDQLHTGRYYIKQFLSNWFGIE
KEEFSTYLRITEDDGAMEHLFRVSTGLDSMVLGETQILGQVKQAFLNSQQVNTTGTIFNELFKQAITFGKRAHKETAIGE
HAVSISYAAVELAKKIFGDLQEKHVAILGAGKMGELAAKNIQGSGATKITVVNRTLENANEMAEKFNADVESIDQLPVIL
QQADILISSTGADSIVVTKEMMEKVQKQRKGRALFLVDIAVPRDMDPAISELENVFLYDIDNLQHIVDDNLESRKQAAEK
IELLIEEEIVTFKEWLKTLGVIPVISALRQKALTIQAETMQSIERKIPNLTDRERKVLNKHTKSIINQLLKEPVTQAKEF
AGKDNAEDSLQLFINIFGIEEEVKEELVKHAKKNETLMKIAKEEPSSFPIIEKITTA
>Mature_457_residues
MHILKVGFNYKTTPVDIREKFTFSEDSLQDAMVELKNQKSILEDVIISTCNRTEIYAVVDQLHTGRYYIKQFLSNWFGIE
KEEFSTYLRITEDDGAMEHLFRVSTGLDSMVLGETQILGQVKQAFLNSQQVNTTGTIFNELFKQAITFGKRAHKETAIGE
HAVSISYAAVELAKKIFGDLQEKHVAILGAGKMGELAAKNIQGSGATKITVVNRTLENANEMAEKFNADVESIDQLPVIL
QQADILISSTGADSIVVTKEMMEKVQKQRKGRALFLVDIAVPRDMDPAISELENVFLYDIDNLQHIVDDNLESRKQAAEK
IELLIEEEIVTFKEWLKTLGVIPVISALRQKALTIQAETMQSIERKIPNLTDRERKVLNKHTKSIINQLLKEPVTQAKEF
AGKDNAEDSLQLFINIFGIEEEVKEELVKHAKKNETLMKIAKEEPSSFPIIEKITTA

Specific function: Catalyzes the NADPH-dependent reduction of glutamyl- tRNA(Glu) to glutamate 1-semialdehyde (GSA)

COG id: COG0373

COG function: function code H; Glutamyl-tRNA reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamyl-tRNA reductase family

Homologues:

Organism=Escherichia coli, GI1787461, Length=420, Percent_Identity=34.2857142857143, Blast_Score=251, Evalue=5e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM1_OCEIH (Q8EPM7)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692991.1
- ProteinModelPortal:   Q8EPM7
- SMR:   Q8EPM7
- GeneID:   1017794
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2070
- NMPDR:   fig|221109.1.peg.2068
- HOGENOM:   HBG732626
- OMA:   GPILNRL
- ProtClustDB:   PRK00045
- BioCyc:   OIHE221109:OB2070-MONOMER
- BRENDA:   1.2.1.70
- GO:   GO:0005737
- HAMAP:   MF_00087
- InterPro:   IPR000343
- InterPro:   IPR015896
- InterPro:   IPR015895
- InterPro:   IPR016040
- InterPro:   IPR018214
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000445
- TIGRFAMs:   TIGR01035

Pfam domain/function: PF00745 GlutR_dimer; PF05201 GlutR_N; PF01488 Shikimate_DH; SSF69075 4pyrrol_synth_GluRdtase_C; SSF69742 GlutR

EC number: =1.2.1.70

Molecular weight: Translated: 51545; Mature: 51545

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00747 GLUTR

Important sites: ACT_SITE 50-50 BINDING 109-109 BINDING 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHILKVGFNYKTTPVDIREKFTFSEDSLQDAMVELKNQKSILEDVIISTCNRTEIYAVVD
CEEEEECCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QLHTGRYYIKQFLSNWFGIEKEEFSTYLRITEDDGAMEHLFRVSTGLDSMVLGETQILGQ
HHHCCHHHHHHHHHHHHCCCHHHHHHEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHH
VKQAFLNSQQVNTTGTIFNELFKQAITFGKRAHKETAIGEHAVSISYAAVELAKKIFGDL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QEKHVAILGAGKMGELAAKNIQGSGATKITVVNRTLENANEMAEKFNADVESIDQLPVIL
HHCCEEEEECCCHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHH
QQADILISSTGADSIVVTKEMMEKVQKQRKGRALFLVDIAVPRDMDPAISELENVFLYDI
HHHHHEEECCCCCCEEEHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHEEEEEH
DNLQHIVDDNLESRKQAAEKIELLIEEEIVTFKEWLKTLGVIPVISALRQKALTIQAETM
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
QSIERKIPNLTDRERKVLNKHTKSIINQLLKEPVTQAKEFAGKDNAEDSLQLFINIFGIE
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCH
EEVKEELVKHAKKNETLMKIAKEEPSSFPIIEKITTA
HHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHCCC
>Mature Secondary Structure
MHILKVGFNYKTTPVDIREKFTFSEDSLQDAMVELKNQKSILEDVIISTCNRTEIYAVVD
CEEEEECCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QLHTGRYYIKQFLSNWFGIEKEEFSTYLRITEDDGAMEHLFRVSTGLDSMVLGETQILGQ
HHHCCHHHHHHHHHHHHCCCHHHHHHEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHH
VKQAFLNSQQVNTTGTIFNELFKQAITFGKRAHKETAIGEHAVSISYAAVELAKKIFGDL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QEKHVAILGAGKMGELAAKNIQGSGATKITVVNRTLENANEMAEKFNADVESIDQLPVIL
HHCCEEEEECCCHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHH
QQADILISSTGADSIVVTKEMMEKVQKQRKGRALFLVDIAVPRDMDPAISELENVFLYDI
HHHHHEEECCCCCCEEEHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHEEEEEH
DNLQHIVDDNLESRKQAAEKIELLIEEEIVTFKEWLKTLGVIPVISALRQKALTIQAETM
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
QSIERKIPNLTDRERKVLNKHTKSIINQLLKEPVTQAKEFAGKDNAEDSLQLFINIFGIE
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCH
EEVKEELVKHAKKNETLMKIAKEEPSSFPIIEKITTA
HHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376