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The map label for this gene is folC [H]

Identifier: 23099513

GI number: 23099513

Start: 2083516

End: 2084796

Strand: Reverse

Name: folC [H]

Synonym: OB2058

Alternate gene names: 23099513

Gene position: 2084796-2083516 (Counterclockwise)

Preceding gene: 23099516

Following gene: 23099512

Centisome position: 57.42

GC content: 32.94

Gene sequence:

>1281_bases
ATGTTATTTGAACAAATAGAACAATATTTTAATAATAGAGAAACAATAGGGGTACAACCTGGATTAGAAAGAGTGAAGGA
ATTATTGAAGTTTGTCGACTATCCAGATAAAGACCTTCCCACTATTCATATAGCTGGTACAAATGGAAAAGGTTCTACGA
GTCAATTCATTCAATCTGCTCTCATAGCTAATGATTATAAGGTTGGATCTTTTACATCTCCTAGTTTTTATGGAATAAGA
GGATTTATTCAAATGAATAACGAGCCAGTATCGGAGAAGGTATTTTCACAAGCATTTCAAAAAATGTTGCCTGCCATTGA
AATGCTAGACAAAGATGGGAACTCCCCGAGTAACTTTGAGATATTAACGGTATTAAGCTTTGTTTGTTTTGAAAATAATG
TAGATATCGCAGTTGTCGAAGCGGGCATGGGAGGAATGTGGGATACGACTAATGTAATTTCGCCAATTGTAACTGTTATC
ACTACTATTTCTTATGACCATACTGCATTGTTAGGTGAAACGTTAGTAGAAATTGCTGAACAAAAAGCGGGTATAATCAA
GCCGTTAATTCCAATGATAACTGGTGTTAACGGAACTGCGTATGACATAATAGAGAAGAAAACGGAAGAGATGAATTCAC
CATTATTTGTTTTAGACAAAGATTTTACGTATGAATCTATAACTATGAATCATTTTAAATTTAATATATCTAATCAACAA
TTTGATATAGATTTAGATATGAATGGAAAACATCAACACTATAATGCTTCTATAGCCCTTTATGTATTACATATACTTCA
TGAACAAGGCTGGCAGTTAACGGAAGATAAGATAATTAAAGGGGTAGAACAAGCTAGATTACCTGGAAGATTAGAGAAAA
TCCATAGTAATCCGTCAATAATTGTAGATGGCGCACATAATATGGAAGGTATACATTCTTTGATAGACACGATAAAACGA
CAGGATTCTAAAAGCGGTCATCATATTTTATTTGCAGCTTTTAAGGATAAAGACGTAAGAAACATGATAAATTGTCTGCA
ATCAGACTTGTCGAACGTTAATGTAACAACATTTGATCATGTTCGCGCTCCCTCCTTTGAGGAATTAGAACAATTAGAAA
CAAAGCAGCCAAAACGAATCGTTTATGATTGGAAAAAATACTTGGAAACAATTTTTGAAAATAAGGATGAATGCTATTAT
ATTACTGGATCTTTACATTTCATATCATTAGTAAAACAATATGTTAATGATCTGCAGCGAAATATGTTGAACGATTCTTA
G

Upstream 100 bases:

>100_bases
TTTAGATTGCCATTGACAATAATCCACCCAGTCTTTTTCATTAAACGAATCATCCTTTCTGCTATAATTATTATATGATG
GAATGGAAAGTAGGGAAAGT

Downstream 100 bases:

>100_bases
AACTAGTGTCAGATTTCGTCTTACTTTTTATATCTGCTGTGCTAGAGTGAGGGACATACTACTGGGCACAGGAGGAACAT
ATTGTGGAGAAAAAGAAACC

Product: folyl-polyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MLFEQIEQYFNNRETIGVQPGLERVKELLKFVDYPDKDLPTIHIAGTNGKGSTSQFIQSALIANDYKVGSFTSPSFYGIR
GFIQMNNEPVSEKVFSQAFQKMLPAIEMLDKDGNSPSNFEILTVLSFVCFENNVDIAVVEAGMGGMWDTTNVISPIVTVI
TTISYDHTALLGETLVEIAEQKAGIIKPLIPMITGVNGTAYDIIEKKTEEMNSPLFVLDKDFTYESITMNHFKFNISNQQ
FDIDLDMNGKHQHYNASIALYVLHILHEQGWQLTEDKIIKGVEQARLPGRLEKIHSNPSIIVDGAHNMEGIHSLIDTIKR
QDSKSGHHILFAAFKDKDVRNMINCLQSDLSNVNVTTFDHVRAPSFEELEQLETKQPKRIVYDWKKYLETIFENKDECYY
ITGSLHFISLVKQYVNDLQRNMLNDS

Sequences:

>Translated_426_residues
MLFEQIEQYFNNRETIGVQPGLERVKELLKFVDYPDKDLPTIHIAGTNGKGSTSQFIQSALIANDYKVGSFTSPSFYGIR
GFIQMNNEPVSEKVFSQAFQKMLPAIEMLDKDGNSPSNFEILTVLSFVCFENNVDIAVVEAGMGGMWDTTNVISPIVTVI
TTISYDHTALLGETLVEIAEQKAGIIKPLIPMITGVNGTAYDIIEKKTEEMNSPLFVLDKDFTYESITMNHFKFNISNQQ
FDIDLDMNGKHQHYNASIALYVLHILHEQGWQLTEDKIIKGVEQARLPGRLEKIHSNPSIIVDGAHNMEGIHSLIDTIKR
QDSKSGHHILFAAFKDKDVRNMINCLQSDLSNVNVTTFDHVRAPSFEELEQLETKQPKRIVYDWKKYLETIFENKDECYY
ITGSLHFISLVKQYVNDLQRNMLNDS
>Mature_426_residues
MLFEQIEQYFNNRETIGVQPGLERVKELLKFVDYPDKDLPTIHIAGTNGKGSTSQFIQSALIANDYKVGSFTSPSFYGIR
GFIQMNNEPVSEKVFSQAFQKMLPAIEMLDKDGNSPSNFEILTVLSFVCFENNVDIAVVEAGMGGMWDTTNVISPIVTVI
TTISYDHTALLGETLVEIAEQKAGIIKPLIPMITGVNGTAYDIIEKKTEEMNSPLFVLDKDFTYESITMNHFKFNISNQQ
FDIDLDMNGKHQHYNASIALYVLHILHEQGWQLTEDKIIKGVEQARLPGRLEKIHSNPSIIVDGAHNMEGIHSLIDTIKR
QDSKSGHHILFAAFKDKDVRNMINCLQSDLSNVNVTTFDHVRAPSFEELEQLETKQPKRIVYDWKKYLETIFENKDECYY
ITGSLHFISLVKQYVNDLQRNMLNDS

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=344, Percent_Identity=29.0697674418605, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI66932990, Length=344, Percent_Identity=29.0697674418605, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI1788654, Length=420, Percent_Identity=24.7619047619048, Blast_Score=114, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71984923, Length=451, Percent_Identity=26.1640798226164, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17553150, Length=451, Percent_Identity=26.1640798226164, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17553148, Length=451, Percent_Identity=26.1640798226164, Blast_Score=138, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6323760, Length=366, Percent_Identity=28.9617486338798, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6324815, Length=442, Percent_Identity=27.6018099547511, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6322718, Length=288, Percent_Identity=25, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24641571, Length=300, Percent_Identity=29.6666666666667, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24581568, Length=202, Percent_Identity=31.1881188118812, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 48434; Mature: 48434

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFEQIEQYFNNRETIGVQPGLERVKELLKFVDYPDKDLPTIHIAGTNGKGSTSQFIQSA
CCHHHHHHHHCCCCEECCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHH
LIANDYKVGSFTSPSFYGIRGFIQMNNEPVSEKVFSQAFQKMLPAIEMLDKDGNSPSNFE
HHHCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
ILTVLSFVCFENNVDIAVVEAGMGGMWDTTNVISPIVTVITTISYDHTALLGETLVEIAE
HHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
QKAGIIKPLIPMITGVNGTAYDIIEKKTEEMNSPLFVLDKDFTYESITMNHFKFNISNQQ
HHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEEEEEEECCCE
FDIDLDMNGKHQHYNASIALYVLHILHEQGWQLTEDKIIKGVEQARLPGRLEKIHSNPSI
EEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCE
IVDGAHNMEGIHSLIDTIKRQDSKSGHHILFAAFKDKDVRNMINCLQSDLSNVNVTTFDH
EEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCEEEECC
VRAPSFEELEQLETKQPKRIVYDWKKYLETIFENKDECYYITGSLHFISLVKQYVNDLQR
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHH
NMLNDS
HHCCCC
>Mature Secondary Structure
MLFEQIEQYFNNRETIGVQPGLERVKELLKFVDYPDKDLPTIHIAGTNGKGSTSQFIQSA
CCHHHHHHHHCCCCEECCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHH
LIANDYKVGSFTSPSFYGIRGFIQMNNEPVSEKVFSQAFQKMLPAIEMLDKDGNSPSNFE
HHHCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
ILTVLSFVCFENNVDIAVVEAGMGGMWDTTNVISPIVTVITTISYDHTALLGETLVEIAE
HHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
QKAGIIKPLIPMITGVNGTAYDIIEKKTEEMNSPLFVLDKDFTYESITMNHFKFNISNQQ
HHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEEEEEEECCCE
FDIDLDMNGKHQHYNASIALYVLHILHEQGWQLTEDKIIKGVEQARLPGRLEKIHSNPSI
EEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCE
IVDGAHNMEGIHSLIDTIKRQDSKSGHHILFAAFKDKDVRNMINCLQSDLSNVNVTTFDH
EEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCEEEECC
VRAPSFEELEQLETKQPKRIVYDWKKYLETIFENKDECYYITGSLHFISLVKQYVNDLQR
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHH
NMLNDS
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]