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The map label for this gene is secDF [H]

Identifier: 23099482

GI number: 23099482

Start: 2055149

End: 2057395

Strand: Reverse

Name: secDF [H]

Synonym: OB2027

Alternate gene names: 23099482

Gene position: 2057395-2055149 (Counterclockwise)

Preceding gene: 23099483

Following gene: 23099481

Centisome position: 56.67

GC content: 35.78

Gene sequence:

>2247_bases
ATGAAAAAAAGAGGTAGAATCGTTGCCTTTTTTCTTGTTGTATTAGTATTGGCAGGAGTAATTGGAACAACGTTTACCAA
TATCACAAAGGATGTAAACCTCGGGCTCGATTTGCAAGGAGGTTTTGAGGTTTTATATGAAGTAGAACCAGTAGATCAAT
CTCAAGAAGTGTCACAAAGTTTATTAGAAGGAACAGTTCAAACACTGAATGATAGGGTAAACCGTCTAGGTATCAGTGAA
GCTGTAATTGATATCGAAGGTGATAATCGAATTCGTGTTCAATTAGCAGGAGTAGATAATCAGGCAGAAGCTAGAGAAAT
GCTATCAACCTCTGCAAATCTAACCTTTCGTGACGTCAATGATAATTTGTATTTGGACGGTACTGACCTCGTGGAGGGAA
GTGCAAAGCAAGATTTTGATCCGAATACAAACGCTCCCATAGTAACATTACAATTAAAAGATGCATCTAAATTTGGTGAT
GTTACAACTGAAATTAGTGAAATGCAAGATAGTCGTTTAGTCATTTGGATGGATTATCAAGAAGGAGATTCATTTGAAGA
AGAAGCTGCTAAAGCTGAACCTAAATATGTATCAGCTCCAAATGTAGATGGTCCCATTAATTCTAGTAATGTAATGATTA
ATGGTAATTTTACGGTTGAGTCTGCACAGCAAATGGCAGATATTATTAATTCTGGTTCCTTACCAGTACATATGACTGAA
ATATTTTCAACTTCTGTTGGTGCCCAGTTTGGTGAACAGGCATTAAACGAGACAGTGTTTGCAGGTATTGTTGGTGTAGG
ATTAATTATCTTATTTATGATTGGTTTCTATCGTTTTACTGGAATAATTGCTGCAATAAATCTTGCAGTATACGTATATT
TGGTTATCTTAGTTTTTGAGATGCTAAATGGCGTTTTAACATTACCAGGTATTGCTGCATTAATACTTGGTGTTGGTATG
GCAGTTGACGCTAATGTAATCACGTTTGAACGAATAAAAGAAGAACTTCGACTAGGGAAATCCGTGAAGGCTGCCTTTAA
ATCAGGTAATTCAAATTCTTTTATCACTATACTAGATGCGAATATAACAACTTTAATCGCAGCTGTTGTATTATTCATTT
TCGGGACAAGTTCCGTAAAGGGATTTGCGACAATGCTAATAATCAGTATAATTTTAAGCTTCCTCACAGCAGTCCTTGGG
ACACGAGTATTACTCGGATTATGGGTGCAAAGTGGATTTCTTAAAAACCGTAAAGCATGGCTAGGCGTTAATAAAACACA
AATCCAAGATATTGCAAAAGCGGAAGAAGAACCAAAAATCTTAGGACGTTCCATCAATTTTGTCAAACATCGAAAGAAAT
TTTTTATTACAACTTCGATTATTGTAATTGTTGGTGCAATCGCAATTGGGATTTTTCGAATAAATCCAGGTGTTGATTTT
ACAAGTGGTTCTAGGGTAGAGGTGGTTGCTGATCAGTCTCTAACCGCTGAAGAAGTGGAAGATCAATTTGCAGAGCTTGG
TCATGAAGTTAACTCCGTAAATTTATCAGGAGAAAATAATCAAATCGCAGTTGCACGTTTTGACACTGTACTTGATGAAT
CTGGTGTGGATGAAGTTCAACAGCATTTTAATGAACTCTATGGTCAAACACCAAGTATAAGTGTTGTTTCACCAATAGTA
GGACAAGAATTAGTAAAAAATGCAATTTATGCAGTATCAATAGCATCTATTTTCATGATAGCTTACGTAACCATTCGATT
TGAATTTTTCTTTGGTATTACCGCGGTTGTTGCATTATTGCATGATGTATTTCTAATGCTTGTATTATTCAGTGTCACTC
AAATTGAATTTAACGTTACGATTGTCGCTGCAATCTTAACTATTGTTGGTTACTCGATTAATAATACGATTGTTATATTT
GATCGTATTCGTGAAAATATACGTCGTGAAAAACGAGTGAAAACCTTCAAAGTATTAGCACGAGTTGTTAACAGAAGTAT
TGTACAATCTTTTACAAGAACGATGAATACTTCAGTGACAACAATTATCGCTGTACTAGCATTTCTGGTTTTAGGCGCTA
CGTCTATTTGGAGTTTTGCACTTGCTTTACTCATCGGGTTATTAGCCGGAACTTATTCTTCTCTATTCTTTGCATCACAA
CTGTGGATGGTTTGGAGAGGGCGTACAGTGAAAAATAAACCAGTAGATTTCACGAAAAAAGAAAAAGTAGAAGGACCGCA
AGTATAA

Upstream 100 bases:

>100_bases
AAAATGAGGACTTGCTGGCATCTATTGCTGCATTGACGGAGAATAATGTAGTTGAAGACGAATAACAATCGCACTAGATT
TGAAGGAGGGAATGGGTTCA

Downstream 100 bases:

>100_bases
TTTTTTAGCCTAGGTTGGGACATACAAAAAGTTTTATTCAAATGATGAACAATTTTAAAAAGTAGCTATGGTATTATCTA
GATTGATCTCAAAGCTATGT

Product: bifunctional preprotein translocase subunit SecD/SecF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 748; Mature: 748

Protein sequence:

>748_residues
MKKRGRIVAFFLVVLVLAGVIGTTFTNITKDVNLGLDLQGGFEVLYEVEPVDQSQEVSQSLLEGTVQTLNDRVNRLGISE
AVIDIEGDNRIRVQLAGVDNQAEAREMLSTSANLTFRDVNDNLYLDGTDLVEGSAKQDFDPNTNAPIVTLQLKDASKFGD
VTTEISEMQDSRLVIWMDYQEGDSFEEEAAKAEPKYVSAPNVDGPINSSNVMINGNFTVESAQQMADIINSGSLPVHMTE
IFSTSVGAQFGEQALNETVFAGIVGVGLIILFMIGFYRFTGIIAAINLAVYVYLVILVFEMLNGVLTLPGIAALILGVGM
AVDANVITFERIKEELRLGKSVKAAFKSGNSNSFITILDANITTLIAAVVLFIFGTSSVKGFATMLIISIILSFLTAVLG
TRVLLGLWVQSGFLKNRKAWLGVNKTQIQDIAKAEEEPKILGRSINFVKHRKKFFITTSIIVIVGAIAIGIFRINPGVDF
TSGSRVEVVADQSLTAEEVEDQFAELGHEVNSVNLSGENNQIAVARFDTVLDESGVDEVQQHFNELYGQTPSISVVSPIV
GQELVKNAIYAVSIASIFMIAYVTIRFEFFFGITAVVALLHDVFLMLVLFSVTQIEFNVTIVAAILTIVGYSINNTIVIF
DRIRENIRREKRVKTFKVLARVVNRSIVQSFTRTMNTSVTTIIAVLAFLVLGATSIWSFALALLIGLLAGTYSSLFFASQ
LWMVWRGRTVKNKPVDFTKKEKVEGPQV

Sequences:

>Translated_748_residues
MKKRGRIVAFFLVVLVLAGVIGTTFTNITKDVNLGLDLQGGFEVLYEVEPVDQSQEVSQSLLEGTVQTLNDRVNRLGISE
AVIDIEGDNRIRVQLAGVDNQAEAREMLSTSANLTFRDVNDNLYLDGTDLVEGSAKQDFDPNTNAPIVTLQLKDASKFGD
VTTEISEMQDSRLVIWMDYQEGDSFEEEAAKAEPKYVSAPNVDGPINSSNVMINGNFTVESAQQMADIINSGSLPVHMTE
IFSTSVGAQFGEQALNETVFAGIVGVGLIILFMIGFYRFTGIIAAINLAVYVYLVILVFEMLNGVLTLPGIAALILGVGM
AVDANVITFERIKEELRLGKSVKAAFKSGNSNSFITILDANITTLIAAVVLFIFGTSSVKGFATMLIISIILSFLTAVLG
TRVLLGLWVQSGFLKNRKAWLGVNKTQIQDIAKAEEEPKILGRSINFVKHRKKFFITTSIIVIVGAIAIGIFRINPGVDF
TSGSRVEVVADQSLTAEEVEDQFAELGHEVNSVNLSGENNQIAVARFDTVLDESGVDEVQQHFNELYGQTPSISVVSPIV
GQELVKNAIYAVSIASIFMIAYVTIRFEFFFGITAVVALLHDVFLMLVLFSVTQIEFNVTIVAAILTIVGYSINNTIVIF
DRIRENIRREKRVKTFKVLARVVNRSIVQSFTRTMNTSVTTIIAVLAFLVLGATSIWSFALALLIGLLAGTYSSLFFASQ
LWMVWRGRTVKNKPVDFTKKEKVEGPQV
>Mature_748_residues
MKKRGRIVAFFLVVLVLAGVIGTTFTNITKDVNLGLDLQGGFEVLYEVEPVDQSQEVSQSLLEGTVQTLNDRVNRLGISE
AVIDIEGDNRIRVQLAGVDNQAEAREMLSTSANLTFRDVNDNLYLDGTDLVEGSAKQDFDPNTNAPIVTLQLKDASKFGD
VTTEISEMQDSRLVIWMDYQEGDSFEEEAAKAEPKYVSAPNVDGPINSSNVMINGNFTVESAQQMADIINSGSLPVHMTE
IFSTSVGAQFGEQALNETVFAGIVGVGLIILFMIGFYRFTGIIAAINLAVYVYLVILVFEMLNGVLTLPGIAALILGVGM
AVDANVITFERIKEELRLGKSVKAAFKSGNSNSFITILDANITTLIAAVVLFIFGTSSVKGFATMLIISIILSFLTAVLG
TRVLLGLWVQSGFLKNRKAWLGVNKTQIQDIAKAEEEPKILGRSINFVKHRKKFFITTSIIVIVGAIAIGIFRINPGVDF
TSGSRVEVVADQSLTAEEVEDQFAELGHEVNSVNLSGENNQIAVARFDTVLDESGVDEVQQHFNELYGQTPSISVVSPIV
GQELVKNAIYAVSIASIFMIAYVTIRFEFFFGITAVVALLHDVFLMLVLFSVTQIEFNVTIVAAILTIVGYSINNTIVIF
DRIRENIRREKRVKTFKVLARVVNRSIVQSFTRTMNTSVTTIIAVLAFLVLGATSIWSFALALLIGLLAGTYSSLFFASQ
LWMVWRGRTVKNKPVDFTKKEKVEGPQV

Specific function: Required for efficient translocation of secretory pre- proteins under conditions of hypersecretion but is not required for the release of mature proteins from the membrane [H]

COG id: COG0342

COG function: function code U; Preprotein translocase subunit SecD

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the secD/secF family. SecF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786609, Length=391, Percent_Identity=30.4347826086957, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI1786610, Length=288, Percent_Identity=31.25, Blast_Score=139, Evalue=6e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005791
- InterPro:   IPR022813
- InterPro:   IPR022646
- InterPro:   IPR022645
- InterPro:   IPR005665 [H]

Pfam domain/function: PF07549 Sec_GG; PF02355 SecD_SecF [H]

EC number: NA

Molecular weight: Translated: 81884; Mature: 81884

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRGRIVAFFLVVLVLAGVIGTTFTNITKDVNLGLDLQGGFEVLYEVEPVDQSQEVSQS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEECCCCCHHHHHHH
LLEGTVQTLNDRVNRLGISEAVIDIEGDNRIRVQLAGVDNQAEAREMLSTSANLTFRDVN
HHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEECCCCHHHHHHHHHCCCCEEEEECC
DNLYLDGTDLVEGSAKQDFDPNTNAPIVTLQLKDASKFGDVTTEISEMQDSRLVIWMDYQ
CCEEECCCHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCEEEEEEECC
EGDSFEEEAAKAEPKYVSAPNVDGPINSSNVMINGNFTVESAQQMADIINSGSLPVHMTE
CCCCHHHHHHCCCCCEECCCCCCCCCCCCCEEEECCEEHHHHHHHHHHHCCCCCCEEHHH
IFSTSVGAQFGEQALNETVFAGIVGVGLIILFMIGFYRFTGIIAAINLAVYVYLVILVFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLNGVLTLPGIAALILGVGMAVDANVITFERIKEELRLGKSVKAAFKSGNSNSFITILDA
HHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHCCCCCCEEEEECC
NITTLIAAVVLFIFGTSSVKGFATMLIISIILSFLTAVLGTRVLLGLWVQSGFLKNRKAW
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LGVNKTQIQDIAKAEEEPKILGRSINFVKHRKKFFITTSIIVIVGAIAIGIFRINPGVDF
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCC
TSGSRVEVVADQSLTAEEVEDQFAELGHEVNSVNLSGENNQIAVARFDTVLDESGVDEVQ
CCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEECCCCCEEEEEEHHHHHCCCCHHHHH
QHFNELYGQTPSISVVSPIVGQELVKNAIYAVSIASIFMIAYVTIRFEFFFGITAVVALL
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HDVFLMLVLFSVTQIEFNVTIVAAILTIVGYSINNTIVIFDRIRENIRREKRVKTFKVLA
HHHHHHHHHHHHHHHEEHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
RVVNRSIVQSFTRTMNTSVTTIIAVLAFLVLGATSIWSFALALLIGLLAGTYSSLFFASQ
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LWMVWRGRTVKNKPVDFTKKEKVEGPQV
HHHHHCCCCCCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure
MKKRGRIVAFFLVVLVLAGVIGTTFTNITKDVNLGLDLQGGFEVLYEVEPVDQSQEVSQS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEECCCCCHHHHHHH
LLEGTVQTLNDRVNRLGISEAVIDIEGDNRIRVQLAGVDNQAEAREMLSTSANLTFRDVN
HHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEECCCCHHHHHHHHHCCCCEEEEECC
DNLYLDGTDLVEGSAKQDFDPNTNAPIVTLQLKDASKFGDVTTEISEMQDSRLVIWMDYQ
CCEEECCCHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCEEEEEEECC
EGDSFEEEAAKAEPKYVSAPNVDGPINSSNVMINGNFTVESAQQMADIINSGSLPVHMTE
CCCCHHHHHHCCCCCEECCCCCCCCCCCCCEEEECCEEHHHHHHHHHHHCCCCCCEEHHH
IFSTSVGAQFGEQALNETVFAGIVGVGLIILFMIGFYRFTGIIAAINLAVYVYLVILVFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLNGVLTLPGIAALILGVGMAVDANVITFERIKEELRLGKSVKAAFKSGNSNSFITILDA
HHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHCCCCCCEEEEECC
NITTLIAAVVLFIFGTSSVKGFATMLIISIILSFLTAVLGTRVLLGLWVQSGFLKNRKAW
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LGVNKTQIQDIAKAEEEPKILGRSINFVKHRKKFFITTSIIVIVGAIAIGIFRINPGVDF
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCC
TSGSRVEVVADQSLTAEEVEDQFAELGHEVNSVNLSGENNQIAVARFDTVLDESGVDEVQ
CCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEECCCCCEEEEEEHHHHHCCCCHHHHH
QHFNELYGQTPSISVVSPIVGQELVKNAIYAVSIASIFMIAYVTIRFEFFFGITAVVALL
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HDVFLMLVLFSVTQIEFNVTIVAAILTIVGYSINNTIVIFDRIRENIRREKRVKTFKVLA
HHHHHHHHHHHHHHHEEHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
RVVNRSIVQSFTRTMNTSVTTIIAVLAFLVLGATSIWSFALALLIGLLAGTYSSLFFASQ
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LWMVWRGRTVKNKPVDFTKKEKVEGPQV
HHHHHCCCCCCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9694879; 9384377 [H]