Click here to switch to the map view.

The map label for this gene is miaB

Identifier: 23099083

GI number: 23099083

Start: 1667702

End: 1669261

Strand: Direct

Name: miaB

Synonym: OB1628

Alternate gene names: 23099083

Gene position: 1667702-1669261 (Clockwise)

Preceding gene: 23099082

Following gene: 23099084

Centisome position: 45.94

GC content: 36.41

Gene sequence:

>1560_bases
ATGAACGAACAGCAACGCAAACAGCAGTCCCAGATTCGTACAGAACAAGCAAATGTAGATAGAATTAAAAATACGTCAAG
TGAAGATATGATTGCTAAGTATTTCCAACAAGAGTATGAGCCGCAATCGCCTGATATAAAACAAGCTAGAAAGCGTAAAA
GAGAAGATGTTCAATTTCATTATGACTTCTCAATCCCAGAGGATATGGAGAAAATCGGGCACGGGAAGAAATTTTTAATT
CGTACTTACGGTTGTCAAATGAACGAGCATGATACCGAAGTAATGGCAGGAATTCTATCTGAGATGGGATATGAATCGAC
TACTGTTACAGAAGAAGCTGATATAATTCTGTTAAACACTTGTGCAATCCGTGAAAATGCAGAAAATAAAGTGTTTGGTG
AGATTGGTCATTTAAAACCTTTGAAATTAGAAAATCCAGATCTCATCATTGGGGTTTGTGGTTGTATGTCACAGGAAGAA
TCAGTAGTAGATCGAATATTACAGAAACATCAACATATCGATTTGATTTTTGGAACACATAATATTCATCGCCTACCACA
CCTTGTGAAAGAAGCATTGTTTGGAAAAGAAATGATTGTAGAGGTATGGTCTAAAGAAGGCGATATTATTGAAAATTTAC
CAAAAGCTCGTAAAGGCAAAATTAAAGCTTGGGTCAATATTATGTATGGATGTGATAAATTCTGTACGTACTGTATAGTT
CCAATGACCCGCGGGAAAGAAAGAAGTCGTCGACCTAAAGACATTATTCAAGAAGTCCGTCATTTAGTTGCTCAAGGTTA
CCAAGAAGTAACTCTCCTCGGCCAAAATGTAAATGCCTACGGGAAAGATTTTGAAGATATCGAATATGGCCTAGGGGACT
TAATGAATGATATTCATAAAATTGATATCCCGCGTGTTCGATTTACAACATCACATCCTAGAGACTTTGATGATCGATTA
ATTGAAGTATTAGCTCAGGGAGGCAACTTACTAGATCATATACACTTGCCTGTTCAGTCAGGAAGTAGTGAAATCTTGAA
AAAGATGAATCGTAAATATACAAGAGAAGAATATTTAGAGTTGGTTCGAAAAATACGTATTGCAATGCCAAATGCAACGT
TAACTACGGATATTATTGTAGGATTCCCGAATGAAACGGAAGAACAATTTGAAGAAACCATGACCTTAGTAGAAGAGGTT
GGGTTTGAAGCTGCATATACATTTATATACTCCCCTCGTGAAGGCACACCTGCTGCTCGAAAAAAAGATGATGTGCCTGA
GGAAGTAAAAAAACAACGTTTATACCGCTTAAACGAACTAGTTAACAAACAATCTGCGGCATCCATGAAAGATTATGCTG
GAAAGAAAGTAAAAGTACTCGTTGAAGGTGAAAGTAAGAAGGATCCTGAAGTATTAGCTGGTTATACTGAAAAAAATAAA
CTTGTTAATTTCAAGGGACCAAAATCATCAATTGGTAAAATTGTAGAAGTAGAAATAACAGAAACCAAAACATGGTCTTT
AAACGGCGTGATGGTTGAAAATACAGTTGAGGTGAACTAG

Upstream 100 bases:

>100_bases
TTGTGTATAGGCTTACAATATTGTACTATTTGGAAAGAAGCTTTATACTATAGTATTGTACATATGTAACTATGATTCGA
ATGAAGAAAGGGGATACCTG

Downstream 100 bases:

>100_bases
TATGACAGAATATACTCGTAAAGAAGTGTTGGATGAAGCAAAGAAGTTAGCTAATATGTTAGCAAGCACAGAAGAAATTG
ATCGTTTTAAACAAGTAGAA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MNEQQRKQQSQIRTEQANVDRIKNTSSEDMIAKYFQQEYEPQSPDIKQARKRKREDVQFHYDFSIPEDMEKIGHGKKFLI
RTYGCQMNEHDTEVMAGILSEMGYESTTVTEEADIILLNTCAIRENAENKVFGEIGHLKPLKLENPDLIIGVCGCMSQEE
SVVDRILQKHQHIDLIFGTHNIHRLPHLVKEALFGKEMIVEVWSKEGDIIENLPKARKGKIKAWVNIMYGCDKFCTYCIV
PMTRGKERSRRPKDIIQEVRHLVAQGYQEVTLLGQNVNAYGKDFEDIEYGLGDLMNDIHKIDIPRVRFTTSHPRDFDDRL
IEVLAQGGNLLDHIHLPVQSGSSEILKKMNRKYTREEYLELVRKIRIAMPNATLTTDIIVGFPNETEEQFEETMTLVEEV
GFEAAYTFIYSPREGTPAARKKDDVPEEVKKQRLYRLNELVNKQSAASMKDYAGKKVKVLVEGESKKDPEVLAGYTEKNK
LVNFKGPKSSIGKIVEVEITETKTWSLNGVMVENTVEVN

Sequences:

>Translated_519_residues
MNEQQRKQQSQIRTEQANVDRIKNTSSEDMIAKYFQQEYEPQSPDIKQARKRKREDVQFHYDFSIPEDMEKIGHGKKFLI
RTYGCQMNEHDTEVMAGILSEMGYESTTVTEEADIILLNTCAIRENAENKVFGEIGHLKPLKLENPDLIIGVCGCMSQEE
SVVDRILQKHQHIDLIFGTHNIHRLPHLVKEALFGKEMIVEVWSKEGDIIENLPKARKGKIKAWVNIMYGCDKFCTYCIV
PMTRGKERSRRPKDIIQEVRHLVAQGYQEVTLLGQNVNAYGKDFEDIEYGLGDLMNDIHKIDIPRVRFTTSHPRDFDDRL
IEVLAQGGNLLDHIHLPVQSGSSEILKKMNRKYTREEYLELVRKIRIAMPNATLTTDIIVGFPNETEEQFEETMTLVEEV
GFEAAYTFIYSPREGTPAARKKDDVPEEVKKQRLYRLNELVNKQSAASMKDYAGKKVKVLVEGESKKDPEVLAGYTEKNK
LVNFKGPKSSIGKIVEVEITETKTWSLNGVMVENTVEVN
>Mature_519_residues
MNEQQRKQQSQIRTEQANVDRIKNTSSEDMIAKYFQQEYEPQSPDIKQARKRKREDVQFHYDFSIPEDMEKIGHGKKFLI
RTYGCQMNEHDTEVMAGILSEMGYESTTVTEEADIILLNTCAIRENAENKVFGEIGHLKPLKLENPDLIIGVCGCMSQEE
SVVDRILQKHQHIDLIFGTHNIHRLPHLVKEALFGKEMIVEVWSKEGDIIENLPKARKGKIKAWVNIMYGCDKFCTYCIV
PMTRGKERSRRPKDIIQEVRHLVAQGYQEVTLLGQNVNAYGKDFEDIEYGLGDLMNDIHKIDIPRVRFTTSHPRDFDDRL
IEVLAQGGNLLDHIHLPVQSGSSEILKKMNRKYTREEYLELVRKIRIAMPNATLTTDIIVGFPNETEEQFEETMTLVEEV
GFEAAYTFIYSPREGTPAARKKDDVPEEVKKQRLYRLNELVNKQSAASMKDYAGKKVKVLVEGESKKDPEVLAGYTEKNK
LVNFKGPKSSIGKIVEVEITETKTWSLNGVMVENTVEVN

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=490, Percent_Identity=36.530612244898, Blast_Score=303, Evalue=3e-82,
Organism=Homo sapiens, GI28872782, Length=490, Percent_Identity=36.530612244898, Blast_Score=303, Evalue=4e-82,
Organism=Homo sapiens, GI93277076, Length=484, Percent_Identity=30.1652892561983, Blast_Score=184, Evalue=1e-46,
Organism=Escherichia coli, GI1786882, Length=440, Percent_Identity=47.9545454545455, Blast_Score=410, Evalue=1e-116,
Organism=Escherichia coli, GI1787057, Length=465, Percent_Identity=27.5268817204301, Blast_Score=160, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17553146, Length=503, Percent_Identity=33.9960238568588, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI71996771, Length=366, Percent_Identity=28.9617486338798, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI21356207, Length=512, Percent_Identity=35.15625, Blast_Score=301, Evalue=7e-82,
Organism=Drosophila melanogaster, GI19922432, Length=515, Percent_Identity=30.2912621359223, Blast_Score=188, Evalue=6e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_OCEIH (Q8EQR4)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692549.1
- ProteinModelPortal:   Q8EQR4
- SMR:   Q8EQR4
- GeneID:   1018123
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1628
- NMPDR:   fig|221109.1.peg.1630
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- ProtClustDB:   PRK14327
- BioCyc:   OIHE221109:OB1628-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 59630; Mature: 59630

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEQQRKQQSQIRTEQANVDRIKNTSSEDMIAKYFQQEYEPQSPDIKQARKRKREDVQFH
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEEE
YDFSIPEDMEKIGHGKKFLIRTYGCQMNEHDTEVMAGILSEMGYESTTVTEEADIILLNT
ECCCCCHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEECCCCCEEEEEE
CAIRENAENKVFGEIGHLKPLKLENPDLIIGVCGCMSQEESVVDRILQKHQHIDLIFGTH
EECCCCCCCCHHHHHCCCCCCEECCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCC
NIHRLPHLVKEALFGKEMIVEVWSKEGDIIENLPKARKGKIKAWVNIMYGCDKFCTYCIV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHCCCHHHHHHHHHHHHHHHHHEEE
PMTRGKERSRRPKDIIQEVRHLVAQGYQEVTLLGQNVNAYGKDFEDIEYGLGDLMNDIHK
ECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
IDIPRVRFTTSHPRDFDDRLIEVLAQGGNLLDHIHLPVQSGSSEILKKMNRKYTREEYLE
CCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCHHHHHH
LVRKIRIAMPNATLTTDIIVGFPNETEEQFEETMTLVEEVGFEAAYTFIYSPREGTPAAR
HHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCC
KKDDVPEEVKKQRLYRLNELVNKQSAASMKDYAGKKVKVLVEGESKKDPEVLAGYTEKNK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHCCCCCCCC
LVNFKGPKSSIGKIVEVEITETKTWSLNGVMVENTVEVN
EEECCCCHHHCCCEEEEEEECCEEEEECCEEEECEEECC
>Mature Secondary Structure
MNEQQRKQQSQIRTEQANVDRIKNTSSEDMIAKYFQQEYEPQSPDIKQARKRKREDVQFH
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEEE
YDFSIPEDMEKIGHGKKFLIRTYGCQMNEHDTEVMAGILSEMGYESTTVTEEADIILLNT
ECCCCCHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEECCCCCEEEEEE
CAIRENAENKVFGEIGHLKPLKLENPDLIIGVCGCMSQEESVVDRILQKHQHIDLIFGTH
EECCCCCCCCHHHHHCCCCCCEECCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCC
NIHRLPHLVKEALFGKEMIVEVWSKEGDIIENLPKARKGKIKAWVNIMYGCDKFCTYCIV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHCCCHHHHHHHHHHHHHHHHHEEE
PMTRGKERSRRPKDIIQEVRHLVAQGYQEVTLLGQNVNAYGKDFEDIEYGLGDLMNDIHK
ECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
IDIPRVRFTTSHPRDFDDRLIEVLAQGGNLLDHIHLPVQSGSSEILKKMNRKYTREEYLE
CCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCHHHHHH
LVRKIRIAMPNATLTTDIIVGFPNETEEQFEETMTLVEEVGFEAAYTFIYSPREGTPAAR
HHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCC
KKDDVPEEVKKQRLYRLNELVNKQSAASMKDYAGKKVKVLVEGESKKDPEVLAGYTEKNK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHCCCCCCCC
LVNFKGPKSSIGKIVEVEITETKTWSLNGVMVENTVEVN
EEECCCCHHHCCCEEEEEEECCEEEEECCEEEECEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376