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The map label for this gene is pnpA

Identifier: 23099059

GI number: 23099059

Start: 1641436

End: 1643553

Strand: Direct

Name: pnpA

Synonym: OB1604

Alternate gene names: 23099059

Gene position: 1641436-1643553 (Clockwise)

Preceding gene: 23099058

Following gene: 23099060

Centisome position: 45.21

GC content: 38.2

Gene sequence:

>2118_bases
ATGGCAGAAGAAAAACAACTATTCTCCACAGAAATTTCTGGTAAGAAATTTACTGTAGAAGTTGGAGAATTAGCAAAACA
AGCAAATGGTGCTTGTATGATTCATTATGGCGATACATCTGTCCTATCCGTAGCAACTGCTTCCAGTGAACCAAAGGACC
TTCCTTTCTTTCCACTAACAGTAAACTACGAAGAACGACTATATGCAGTCGGTAAGATACCAGGAGGATTTATAAAGCGA
GAAGGGCGTCCGAGTGAAAAAGCGATCCTTTCATCTCGTCTAATAGACCGACCTATCCGTCCATTGTTCCCGGATGGTTT
TCGAAATGAAGTGCAAGTAATTAGTACGGTAATGAGCGTAGATCAAGACTGCCCTTCAGAGATTGCCGCTATGATTGGCT
CTTCAATTGCACTTAGTGTGTCTGATATTCCATTTGAGCAACCAATCGCTGGTGTGAATGTTGGAAGAGTAGACGGGGAA
TTTATTATAAATCCAACAATTGAACAAGAAGCAAAAAGTGACATTGAATTAACAGTCGCTGGTACAAAAGATGCGATTAA
CATGGTAGAAGCAGGAGCAAATGAAGTTCCAGAAGATATTATGTTAGAAGCAATTATGTTTGGCCATGAAGAAATTAAGC
GCTTAGTAGCTTTTCAAGAAGAACTTGTGAAGGCATGTGGACAAGATAAATTTGATGTTGTATTAGCTGAATCTGAAGAA
GAGCTAGTTGAAAATGTTCATTCAGAAGCGAAAGACAAGATTGTAAATGCAATACAGGTACAGGAAAAGCATGCACGTGA
TGAAGCGATAAAACAAGCGAAAAATGAAATTGTTGCTCATTATGAAGAACAAGAAGTCGAAGAAGATGTAATCAAACAAG
TAAAATCAATTTTAGATAGCATGGTGAAGGAAGAAGTTCGTCGACTAATCACGAAAGAAAAAATTAGACCTGATGGACGA
AAAGTGGATGAAATTCGACCACTGTCTTCTAGAATACATGTATTGCCACGTACGCATGGCTCTGGATTATTTACACGTGG
ACAAACACAGGCACTTAGTGTGTGTACTCTTGGTGCACTTGGGGATGTACAAATCTTAGACGGTTTAGACTTAGAAGAAT
CAAAAAGATTTATGCATCATTATAACTTCCCTCAGTATAGTGTCGGGGAAACAGGTCCAATTCGTGGACCAGGTCGTCGT
GAGATAGGCCACGGGGCATTAGGTGAACGTGCTTTAGAAAAAGTAATTCCGGATGACAAAGAATTTCCGTATACGATTCG
ACTTGTATCAGAAGTTTTAGAATCAAATGGTTCTACATCACAGGCAAGTATTTGTGCTAGTACATTAGCAATGATGGATG
CCGGTGTACCAATTAAAGCACCAGTTGCAGGTATTGCAATGGGGCTTGTTAAATCAGGTGATGATTATACGATTCTGACG
GATATTCAAGGTATGGAAGATGCGTTAGGAGATATGGACTTTAAAGTTGCTGGAACCGCTAATGGGGTTACTGCATTGCA
AATGGATATTAAGATTGATGGATTATCTCGTGATATCTTAGAAGAAGCACTTTCTCAAGCGAAGAAAGGTCGCATGCAGA
TTCTAGATTCTATGTTAGCAACAATTAAAGAACCGAAAGAAGAACTATCTGAGTTTGCACCAAAAATCCTAACAATGGCA
ATAGAACCAGATAAGATCCGTGATGTTATTGGACCAAGCGGAAAACAAATCAATCAAATTATTGATGAAACTGGTGTGAA
AATTGATATTGAACAAGATGGAAGTATCTTTATTTCCTCAACAGACAATGAAATGAACAAAAAAGCAAAACAAATCATTG
AAGATCTTGTCCGTGAAGTAGAAGTAGGACAGATTTATCTTGGTAAAGTAAAACGTATCGAGAAATTTGGAGCTTTCGTT
GAATTGTTTAAAGGAAAAGATGGTCTCGTTCACATTTCAGAGCTTGCAGAAGAACGCACGAATAAAGTGGAAGACGTTGT
TTCTATAGACGATCAGATTATGGTTAAAGTAAAAGAAATTGATCGTCAAGGACGTGTGAATTTATCTAGAAAAGCCGTTA
TTCAAGACGAGAAGAAAGCAAAAGAACAAGCAAAATAA

Upstream 100 bases:

>100_bases
GCTATAAAAAGAGAATGTTTTTAGATGTAACACCGAATTACATTTGAATATAGAACTGCTACAGATATATTATCGTAGCA
TGAAAGGAGAACTAGAAATA

Downstream 100 bases:

>100_bases
ATGTATAAAGGCGATAAAAAGAAGCTGAACCATTCTAATTTCAGTTTCTTTTTTGCATGCGTCTTTACTTATGAAAGAAG
AAATACATATCATAGGTTGT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 705; Mature: 704

Protein sequence:

>705_residues
MAEEKQLFSTEISGKKFTVEVGELAKQANGACMIHYGDTSVLSVATASSEPKDLPFFPLTVNYEERLYAVGKIPGGFIKR
EGRPSEKAILSSRLIDRPIRPLFPDGFRNEVQVISTVMSVDQDCPSEIAAMIGSSIALSVSDIPFEQPIAGVNVGRVDGE
FIINPTIEQEAKSDIELTVAGTKDAINMVEAGANEVPEDIMLEAIMFGHEEIKRLVAFQEELVKACGQDKFDVVLAESEE
ELVENVHSEAKDKIVNAIQVQEKHARDEAIKQAKNEIVAHYEEQEVEEDVIKQVKSILDSMVKEEVRRLITKEKIRPDGR
KVDEIRPLSSRIHVLPRTHGSGLFTRGQTQALSVCTLGALGDVQILDGLDLEESKRFMHHYNFPQYSVGETGPIRGPGRR
EIGHGALGERALEKVIPDDKEFPYTIRLVSEVLESNGSTSQASICASTLAMMDAGVPIKAPVAGIAMGLVKSGDDYTILT
DIQGMEDALGDMDFKVAGTANGVTALQMDIKIDGLSRDILEEALSQAKKGRMQILDSMLATIKEPKEELSEFAPKILTMA
IEPDKIRDVIGPSGKQINQIIDETGVKIDIEQDGSIFISSTDNEMNKKAKQIIEDLVREVEVGQIYLGKVKRIEKFGAFV
ELFKGKDGLVHISELAEERTNKVEDVVSIDDQIMVKVKEIDRQGRVNLSRKAVIQDEKKAKEQAK

Sequences:

>Translated_705_residues
MAEEKQLFSTEISGKKFTVEVGELAKQANGACMIHYGDTSVLSVATASSEPKDLPFFPLTVNYEERLYAVGKIPGGFIKR
EGRPSEKAILSSRLIDRPIRPLFPDGFRNEVQVISTVMSVDQDCPSEIAAMIGSSIALSVSDIPFEQPIAGVNVGRVDGE
FIINPTIEQEAKSDIELTVAGTKDAINMVEAGANEVPEDIMLEAIMFGHEEIKRLVAFQEELVKACGQDKFDVVLAESEE
ELVENVHSEAKDKIVNAIQVQEKHARDEAIKQAKNEIVAHYEEQEVEEDVIKQVKSILDSMVKEEVRRLITKEKIRPDGR
KVDEIRPLSSRIHVLPRTHGSGLFTRGQTQALSVCTLGALGDVQILDGLDLEESKRFMHHYNFPQYSVGETGPIRGPGRR
EIGHGALGERALEKVIPDDKEFPYTIRLVSEVLESNGSTSQASICASTLAMMDAGVPIKAPVAGIAMGLVKSGDDYTILT
DIQGMEDALGDMDFKVAGTANGVTALQMDIKIDGLSRDILEEALSQAKKGRMQILDSMLATIKEPKEELSEFAPKILTMA
IEPDKIRDVIGPSGKQINQIIDETGVKIDIEQDGSIFISSTDNEMNKKAKQIIEDLVREVEVGQIYLGKVKRIEKFGAFV
ELFKGKDGLVHISELAEERTNKVEDVVSIDDQIMVKVKEIDRQGRVNLSRKAVIQDEKKAKEQAK
>Mature_704_residues
AEEKQLFSTEISGKKFTVEVGELAKQANGACMIHYGDTSVLSVATASSEPKDLPFFPLTVNYEERLYAVGKIPGGFIKRE
GRPSEKAILSSRLIDRPIRPLFPDGFRNEVQVISTVMSVDQDCPSEIAAMIGSSIALSVSDIPFEQPIAGVNVGRVDGEF
IINPTIEQEAKSDIELTVAGTKDAINMVEAGANEVPEDIMLEAIMFGHEEIKRLVAFQEELVKACGQDKFDVVLAESEEE
LVENVHSEAKDKIVNAIQVQEKHARDEAIKQAKNEIVAHYEEQEVEEDVIKQVKSILDSMVKEEVRRLITKEKIRPDGRK
VDEIRPLSSRIHVLPRTHGSGLFTRGQTQALSVCTLGALGDVQILDGLDLEESKRFMHHYNFPQYSVGETGPIRGPGRRE
IGHGALGERALEKVIPDDKEFPYTIRLVSEVLESNGSTSQASICASTLAMMDAGVPIKAPVAGIAMGLVKSGDDYTILTD
IQGMEDALGDMDFKVAGTANGVTALQMDIKIDGLSRDILEEALSQAKKGRMQILDSMLATIKEPKEELSEFAPKILTMAI
EPDKIRDVIGPSGKQINQIIDETGVKIDIEQDGSIFISSTDNEMNKKAKQIIEDLVREVEVGQIYLGKVKRIEKFGAFVE
LFKGKDGLVHISELAEERTNKVEDVVSIDDQIMVKVKEIDRQGRVNLSRKAVIQDEKKAKEQAK

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=710, Percent_Identity=36.4788732394366, Blast_Score=424, Evalue=1e-118,
Organism=Homo sapiens, GI9506689, Length=235, Percent_Identity=26.3829787234043, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI145693187, Length=679, Percent_Identity=50.0736377025037, Blast_Score=653, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=648, Percent_Identity=34.5679012345679, Blast_Score=342, Evalue=4e-94,
Organism=Drosophila melanogaster, GI281362905, Length=710, Percent_Identity=37.3239436619718, Blast_Score=428, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24651641, Length=710, Percent_Identity=37.3239436619718, Blast_Score=428, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24651643, Length=710, Percent_Identity=37.3239436619718, Blast_Score=428, Evalue=1e-120,
Organism=Drosophila melanogaster, GI161079377, Length=660, Percent_Identity=37.1212121212121, Blast_Score=394, Evalue=1e-109,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_OCEIH (Q8EQT7)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692525.1
- HSSP:   P05055
- ProteinModelPortal:   Q8EQT7
- GeneID:   1017992
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1604
- NMPDR:   fig|221109.1.peg.1606
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   OIHE221109:OB1604-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 77678; Mature: 77547

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEEKQLFSTEISGKKFTVEVGELAKQANGACMIHYGDTSVLSVATASSEPKDLPFFPLT
CCCCHHHHHHCCCCCEEEEEHHHHHHHCCCEEEEEECCCCEEEEEECCCCCCCCCEEEEE
VNYEERLYAVGKIPGGFIKREGRPSEKAILSSRLIDRPIRPLFPDGFRNEVQVISTVMSV
ECHHHHEEEEECCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
DQDCPSEIAAMIGSSIALSVSDIPFEQPIAGVNVGRVDGEFIINPTIEQEAKSDIELTVA
CCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCEECCCCCCEEECCCHHHHCCCCEEEEEE
GTKDAINMVEAGANEVPEDIMLEAIMFGHEEIKRLVAFQEELVKACGQDKFDVVLAESEE
CCHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHH
ELVENVHSEAKDKIVNAIQVQEKHARDEAIKQAKNEIVAHYEEQEVEEDVIKQVKSILDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVKEEVRRLITKEKIRPDGRKVDEIRPLSSRIHVLPRTHGSGLFTRGQTQALSVCTLGAL
HHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHEEEEECCCCCCCEECCCCHHHHHEEECCC
GDVQILDGLDLEESKRFMHHYNFPQYSVGETGPIRGPGRREIGHGALGERALEKVIPDDK
CCCCEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
EFPYTIRLVSEVLESNGSTSQASICASTLAMMDAGVPIKAPVAGIAMGLVKSGDDYTILT
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEE
DIQGMEDALGDMDFKVAGTANGVTALQMDIKIDGLSRDILEEALSQAKKGRMQILDSMLA
CCCCHHHHHCCCCEEEECCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
TIKEPKEELSEFAPKILTMAIEPDKIRDVIGPSGKQINQIIDETGVKIDIEQDGSIFISS
HHHCHHHHHHHHHHHHEEEEECCHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCCEEEEC
TDNEMNKKAKQIIEDLVREVEVGQIYLGKVKRIEKFGAFVELFKGKDGLVHISELAEERT
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHH
NKVEDVVSIDDQIMVKVKEIDRQGRVNLSRKAVIQDEKKAKEQAK
HHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHCC
>Mature Secondary Structure 
AEEKQLFSTEISGKKFTVEVGELAKQANGACMIHYGDTSVLSVATASSEPKDLPFFPLT
CCCHHHHHHCCCCCEEEEEHHHHHHHCCCEEEEEECCCCEEEEEECCCCCCCCCEEEEE
VNYEERLYAVGKIPGGFIKREGRPSEKAILSSRLIDRPIRPLFPDGFRNEVQVISTVMSV
ECHHHHEEEEECCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
DQDCPSEIAAMIGSSIALSVSDIPFEQPIAGVNVGRVDGEFIINPTIEQEAKSDIELTVA
CCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCEECCCCCCEEECCCHHHHCCCCEEEEEE
GTKDAINMVEAGANEVPEDIMLEAIMFGHEEIKRLVAFQEELVKACGQDKFDVVLAESEE
CCHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHH
ELVENVHSEAKDKIVNAIQVQEKHARDEAIKQAKNEIVAHYEEQEVEEDVIKQVKSILDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MVKEEVRRLITKEKIRPDGRKVDEIRPLSSRIHVLPRTHGSGLFTRGQTQALSVCTLGAL
HHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHEEEEECCCCCCCEECCCCHHHHHEEECCC
GDVQILDGLDLEESKRFMHHYNFPQYSVGETGPIRGPGRREIGHGALGERALEKVIPDDK
CCCCEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
EFPYTIRLVSEVLESNGSTSQASICASTLAMMDAGVPIKAPVAGIAMGLVKSGDDYTILT
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEE
DIQGMEDALGDMDFKVAGTANGVTALQMDIKIDGLSRDILEEALSQAKKGRMQILDSMLA
CCCCHHHHHCCCCEEEECCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
TIKEPKEELSEFAPKILTMAIEPDKIRDVIGPSGKQINQIIDETGVKIDIEQDGSIFISS
HHHCHHHHHHHHHHHHEEEEECCHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCCEEEEC
TDNEMNKKAKQIIEDLVREVEVGQIYLGKVKRIEKFGAFVELFKGKDGLVHISELAEERT
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHH
NKVEDVVSIDDQIMVKVKEIDRQGRVNLSRKAVIQDEKKAKEQAK
HHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376