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The map label for this gene is truB

Identifier: 23099056

GI number: 23099056

Start: 1638987

End: 1639898

Strand: Direct

Name: truB

Synonym: OB1601

Alternate gene names: 23099056

Gene position: 1638987-1639898 (Clockwise)

Preceding gene: 23099055

Following gene: 23099057

Centisome position: 45.14

GC content: 35.75

Gene sequence:

>912_bases
ATGCATGGTATTCTTCCGTTATGGAAACCAAAAGGATTAACTTCACATGACTGTGTGATGCGTTGTCGTCGATATTTTAA
GACGAAAAAGGTTGGGCATACTGGAACTTTGGATCCAGAAGTTGAGGGTGTGTTACCTATATGTATTGGACAAGCAACAA
AAATTGTTCCGTTCTTGACAGATACAAAAAAAGTATATGAAGCTACGGTTCAACTAGGATATTCAACCGAGACAGAGGAT
GCAACGGGAAAAATAGTAGAAACAAAGGAAGTTTCTGATTTTCCAACGAACAAGAATTTGGAAGAAGTACTTCAGACTTT
TATAGGAAGAACAAAGCAAATTCCACCAATGTACTCTGCTGTAAAGGTAAACGGAAAAAAGTTATATGAATATGCTAGAG
CAAATGAATCAGTGGAAAGACCTGTACGTGAAATTGAGATATTTGAATTAACGCTTACCAGTGTCGATGAGAAAAATCAT
TCCTTTGACATTCGCATCGTTTGTTCAAAAGGCACTTATATACGTACTCTTTGTGTTGATATAGGAAAAGCACTGGGATA
TCCAGCACATATGTCTTTACTTACTCGTACAAAGACAGGGGCATTTTCTGAGAAAAATACGGTAACTTTTGATATGATAG
AAGAAGCCGTTTCCAATCAATCAGAAGAACGGTTACTTGAACCAATTATAAACGGTTTACAGCATTTGGAACAAATAGAA
GTAAATGAAGATATGGAAAAAAGAATTTTAAATGGTCAGAAACTTTCATTACAAAGAAATCAACCCAAGCAAACAGATCC
GTTTGTATTTGTCAGGGAAGGAAATGTACTGGCTATCTATCAATCTCACCCAACTAATGAAGATCAGATAAAACCAGTAC
GTGTATTTGCAATTGAAGATAAAAAAGTGTAG

Upstream 100 bases:

>100_bases
TTACATAATCGTTCGTGTACATTGAAATCAAATAGGAAAACATCAAATTTTTTACATAATATCTGAACTTGATTCAAGTA
AAGTAGAAGGGAGCTTTACA

Downstream 100 bases:

>100_bases
GTGAAGAAGTTGAGGACAATAGAGTTAACATATCCACATAGCTTGCATATGGAAGAATTACCAAAAACCGTAAGTGCGAT
TGGATTCTTTGATGGTATCC

Product: tRNA-pseudouridine 5S synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MHGILPLWKPKGLTSHDCVMRCRRYFKTKKVGHTGTLDPEVEGVLPICIGQATKIVPFLTDTKKVYEATVQLGYSTETED
ATGKIVETKEVSDFPTNKNLEEVLQTFIGRTKQIPPMYSAVKVNGKKLYEYARANESVERPVREIEIFELTLTSVDEKNH
SFDIRIVCSKGTYIRTLCVDIGKALGYPAHMSLLTRTKTGAFSEKNTVTFDMIEEAVSNQSEERLLEPIINGLQHLEQIE
VNEDMEKRILNGQKLSLQRNQPKQTDPFVFVREGNVLAIYQSHPTNEDQIKPVRVFAIEDKKV

Sequences:

>Translated_303_residues
MHGILPLWKPKGLTSHDCVMRCRRYFKTKKVGHTGTLDPEVEGVLPICIGQATKIVPFLTDTKKVYEATVQLGYSTETED
ATGKIVETKEVSDFPTNKNLEEVLQTFIGRTKQIPPMYSAVKVNGKKLYEYARANESVERPVREIEIFELTLTSVDEKNH
SFDIRIVCSKGTYIRTLCVDIGKALGYPAHMSLLTRTKTGAFSEKNTVTFDMIEEAVSNQSEERLLEPIINGLQHLEQIE
VNEDMEKRILNGQKLSLQRNQPKQTDPFVFVREGNVLAIYQSHPTNEDQIKPVRVFAIEDKKV
>Mature_303_residues
MHGILPLWKPKGLTSHDCVMRCRRYFKTKKVGHTGTLDPEVEGVLPICIGQATKIVPFLTDTKKVYEATVQLGYSTETED
ATGKIVETKEVSDFPTNKNLEEVLQTFIGRTKQIPPMYSAVKVNGKKLYEYARANESVERPVREIEIFELTLTSVDEKNH
SFDIRIVCSKGTYIRTLCVDIGKALGYPAHMSLLTRTKTGAFSEKNTVTFDMIEEAVSNQSEERLLEPIINGLQHLEQIE
VNEDMEKRILNGQKLSLQRNQPKQTDPFVFVREGNVLAIYQSHPTNEDQIKPVRVFAIEDKKV

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=220, Percent_Identity=35.4545454545455, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI215599015, Length=238, Percent_Identity=30.672268907563, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI4503337, Length=238, Percent_Identity=30.672268907563, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI2367200, Length=235, Percent_Identity=40, Blast_Score=177, Evalue=8e-46,
Organism=Caenorhabditis elegans, GI17553978, Length=238, Percent_Identity=27.7310924369748, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323204, Length=261, Percent_Identity=31.8007662835249, Blast_Score=109, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6324037, Length=194, Percent_Identity=32.4742268041237, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI281364189, Length=263, Percent_Identity=27.7566539923954, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281364187, Length=263, Percent_Identity=27.7566539923954, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281364185, Length=263, Percent_Identity=27.7566539923954, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281364183, Length=263, Percent_Identity=27.7566539923954, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI62471759, Length=263, Percent_Identity=27.7566539923954, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17975520, Length=263, Percent_Identity=27.7566539923954, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_OCEIH (Q8CXG9)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692522.1
- ProteinModelPortal:   Q8CXG9
- SMR:   Q8CXG9
- GeneID:   1017989
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1601
- NMPDR:   fig|221109.1.peg.1603
- HOGENOM:   HBG397258
- OMA:   ASTKAYD
- BioCyc:   OIHE221109:OB1601-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 34417; Mature: 34417

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: ACT_SITE 38-38

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGILPLWKPKGLTSHDCVMRCRRYFKTKKVGHTGTLDPEVEGVLPICIGQATKIVPFLT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCHHEEEEEC
DTKKVYEATVQLGYSTETEDATGKIVETKEVSDFPTNKNLEEVLQTFIGRTKQIPPMYSA
CHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHE
VKVNGKKLYEYARANESVERPVREIEIFELTLTSVDEKNHSFDIRIVCSKGTYIRTLCVD
EEECHHHHHHHHHCCCHHHHHHHHEEEEEEEEECCCCCCCCEEEEEEECCCCCHHHHHHH
IGKALGYPAHMSLLTRTKTGAFSEKNTVTFDMIEEAVSNQSEERLLEPIINGLQHLEQIE
HHHHHCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
VNEDMEKRILNGQKLSLQRNQPKQTDPFVFVREGNVLAIYQSHPTNEDQIKPVRVFAIED
CCCHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCEEEEEEECC
KKV
CCC
>Mature Secondary Structure
MHGILPLWKPKGLTSHDCVMRCRRYFKTKKVGHTGTLDPEVEGVLPICIGQATKIVPFLT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCHHEEEEEC
DTKKVYEATVQLGYSTETEDATGKIVETKEVSDFPTNKNLEEVLQTFIGRTKQIPPMYSA
CHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHE
VKVNGKKLYEYARANESVERPVREIEIFELTLTSVDEKNHSFDIRIVCSKGTYIRTLCVD
EEECHHHHHHHHHCCCHHHHHHHHEEEEEEEEECCCCCCCCEEEEEEECCCCCHHHHHHH
IGKALGYPAHMSLLTRTKTGAFSEKNTVTFDMIEEAVSNQSEERLLEPIINGLQHLEQIE
HHHHHCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
VNEDMEKRILNGQKLSLQRNQPKQTDPFVFVREGNVLAIYQSHPTNEDQIKPVRVFAIED
CCCHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCEEEEEEECC
KKV
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376