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The map label for this gene is infB

Identifier: 23099053

GI number: 23099053

Start: 1636093

End: 1638171

Strand: Direct

Name: infB

Synonym: OB1598

Alternate gene names: 23099053

Gene position: 1636093-1638171 (Clockwise)

Preceding gene: 23099052

Following gene: 23099054

Centisome position: 45.06

GC content: 37.09

Gene sequence:

>2079_bases
ATGAGTAAAATACGTATATATGAATATGCAAAACAAAATAATTTAGCAAGTAAAGATGTAATTAACTTCTTAAAAACGGA
AAATGTAGAAGTATCAAATCATATGTCTGCAATTTCTGATGAAGTTGTTAAAAAACTAGATAATAAATTTAAGGCAAAAC
CAGAGAATGCTAAAAAAGGGCAAAATCAAAAGCAATCTAACAACCAACAACAGAATAGACAGAAACAAAATCAAAAAAAT
CAGTCTAAACCGAATAAAAACAAGAAACAAAAAGGTCCCAAGAAAAATCAACAACAAGAGAGACCTGCCGCTAAACCAGC
AGAAACTCCTGGAAAAATAACGTACCATGGTACGTTAACTGTGCAAGATTTAGCTGGAAAGCTTAATAAGGAGCCTGCTG
AACTTATTAAAAAGCTAATGTTCCTTGGAGTAATGGCTACGAAAAACCAAGATATAGATGATGATGCGATAGAGTTAATT
TGCGGTGAGTATGATGTAGAGGTTGAAAAAGAAATCATACTAGAAGATACAGACTTAGATAAATATATTGAAGAAGATGC
AGAAGAAAATCTACAAGAACGTCCAGCTGTTGTAACGATTATGGGACATGTTGACCACGGTAAAACAACTTTGTTAGATT
CTATTCGTCATACGAAAGTTACAGCAGGTGAAGCAGGTGGTATAACACAGCATATCGGTGCGTATCAAGTAGAAAACGAC
GGTAAAAAGATTACTTTCTTAGATACACCTGGACACGCTGCATTTACTAGTATGCGATCTCGTGGTGCACAGGTTACGGA
TATAGCTATATTAGTTGTTGCTGCTGATGATGGTGTAATGCCACAAACAGTAGAAGCGATTAACCACGCTAAAGCAGCAG
AGGTTCCAATTATTGTTGCGGTTAATAAAATGGATAAAGAAGGAGCAAACCCTGATCGTGTCATGCAAGAACTGACTGAG
CATCAATTAATTCCTGAAGATTGGGGCGGCAACACGATTTTTGTTAATCTTTCTGCGATTAAAAATGAAGGTATCGATGA
TTTATTGGAAATGATTCTTCTTGTTTCTGAAGTGGAAGAATTAAAGGCGAATCCGAATGCAAAAGCGTTTGGTAGTGTTA
TTGATGCTCAATTAGATAAAGGAAGAGGTTCTGTTGCAACATTACTCGTTCAAAATGGTACACTTCATGTTGGAGATCCA
TTAGTTGTAGGAAGTACTTTTGGTAAAGTTCGTGCTATGGTAAATGACTTGGGTAATCGTGTAACTGAAGTTGGTCCGTC
TACTCCAGTAGAAATCACTGGTTTACATGGTGTTCCTCAAGCAGGGGACCAGTTCCTTGTCTTTAAAGATGAGAAAAAAG
CTCGTCAAATCGGAGAGGCACGTGAGCAAAAACAAATTGATGAGAATCGTGGAACTCAATCTACCGTAAGCTTAGACGAT
CTATTTGAGCAAATTAAGCAAGGTGAAATGAAAGAATTAAATATTATTGTCAAAGCAGATGTACAAGGTTCTGTGGAAGC
ACTAGCTGCATCTTTACAAAAAATTGAAGTAGAAGGCGTTAACGTTAAAATCATTCACACAGGAGTAGGTGCTATCACAG
AATCAGATATTATACTTGCTTCTGCGTCTAAAGCGATTGTTATTGGATTTAATGTTCGTCCAGACGTGAATGCGAAAAAC
GCTGCAGAGTCAGAAAAAGTAGATCTGCGCCTTCATCGTGTTATCTACAACGCGATTGAAGAAATCGAATCTGCAATGAA
AGGGTTACTCGATCCTGAATATCAAGAGAAAGTAATCGGTCAAGCAGAAGTACGCGAAATCTTTAAAGTTTCTCGAATTG
GTACCATTGCAGGAAGTTATGTAACGGATGGGAAAATTACAAGAGATGCTGGCGTTCGTTTAATTCGTGACGGAGTTGTA
TTATATGAGGGTGAGCTTCAGGCTCTTAAACGATTTAAAGACGATGTGAAAGAGGTTCAAACGAACTACGAGTGTGGTAT
TACCATCTCCAATTTTAATGATATTAAAGAAGGGGATACAATCGAAGCATTCGTTATGGAAGAAATTGAACGTAAATGA

Upstream 100 bases:

>100_bases
TCAATCGGAAAGACCCATAGAGTAGCTGTCGCTATTTTAGATGCAGGCTTTGCCAAGAAGCTCATATCTCTCATTGGGGA
ATAAATTCGGGGGTGAACGT

Downstream 100 bases:

>100_bases
TTGTCTACGCAGAAGTAGAATGTATGCTATATGATGGTCATTCCTTAAAGGATAAGCGTTCGATTATTAAGAAGGTAATA
TCTAAACTTCGCCAGACATC

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 692; Mature: 691

Protein sequence:

>692_residues
MSKIRIYEYAKQNNLASKDVINFLKTENVEVSNHMSAISDEVVKKLDNKFKAKPENAKKGQNQKQSNNQQQNRQKQNQKN
QSKPNKNKKQKGPKKNQQQERPAAKPAETPGKITYHGTLTVQDLAGKLNKEPAELIKKLMFLGVMATKNQDIDDDAIELI
CGEYDVEVEKEIILEDTDLDKYIEEDAEENLQERPAVVTIMGHVDHGKTTLLDSIRHTKVTAGEAGGITQHIGAYQVEND
GKKITFLDTPGHAAFTSMRSRGAQVTDIAILVVAADDGVMPQTVEAINHAKAAEVPIIVAVNKMDKEGANPDRVMQELTE
HQLIPEDWGGNTIFVNLSAIKNEGIDDLLEMILLVSEVEELKANPNAKAFGSVIDAQLDKGRGSVATLLVQNGTLHVGDP
LVVGSTFGKVRAMVNDLGNRVTEVGPSTPVEITGLHGVPQAGDQFLVFKDEKKARQIGEAREQKQIDENRGTQSTVSLDD
LFEQIKQGEMKELNIIVKADVQGSVEALAASLQKIEVEGVNVKIIHTGVGAITESDIILASASKAIVIGFNVRPDVNAKN
AAESEKVDLRLHRVIYNAIEEIESAMKGLLDPEYQEKVIGQAEVREIFKVSRIGTIAGSYVTDGKITRDAGVRLIRDGVV
LYEGELQALKRFKDDVKEVQTNYECGITISNFNDIKEGDTIEAFVMEEIERK

Sequences:

>Translated_692_residues
MSKIRIYEYAKQNNLASKDVINFLKTENVEVSNHMSAISDEVVKKLDNKFKAKPENAKKGQNQKQSNNQQQNRQKQNQKN
QSKPNKNKKQKGPKKNQQQERPAAKPAETPGKITYHGTLTVQDLAGKLNKEPAELIKKLMFLGVMATKNQDIDDDAIELI
CGEYDVEVEKEIILEDTDLDKYIEEDAEENLQERPAVVTIMGHVDHGKTTLLDSIRHTKVTAGEAGGITQHIGAYQVEND
GKKITFLDTPGHAAFTSMRSRGAQVTDIAILVVAADDGVMPQTVEAINHAKAAEVPIIVAVNKMDKEGANPDRVMQELTE
HQLIPEDWGGNTIFVNLSAIKNEGIDDLLEMILLVSEVEELKANPNAKAFGSVIDAQLDKGRGSVATLLVQNGTLHVGDP
LVVGSTFGKVRAMVNDLGNRVTEVGPSTPVEITGLHGVPQAGDQFLVFKDEKKARQIGEAREQKQIDENRGTQSTVSLDD
LFEQIKQGEMKELNIIVKADVQGSVEALAASLQKIEVEGVNVKIIHTGVGAITESDIILASASKAIVIGFNVRPDVNAKN
AAESEKVDLRLHRVIYNAIEEIESAMKGLLDPEYQEKVIGQAEVREIFKVSRIGTIAGSYVTDGKITRDAGVRLIRDGVV
LYEGELQALKRFKDDVKEVQTNYECGITISNFNDIKEGDTIEAFVMEEIERK
>Mature_691_residues
SKIRIYEYAKQNNLASKDVINFLKTENVEVSNHMSAISDEVVKKLDNKFKAKPENAKKGQNQKQSNNQQQNRQKQNQKNQ
SKPNKNKKQKGPKKNQQQERPAAKPAETPGKITYHGTLTVQDLAGKLNKEPAELIKKLMFLGVMATKNQDIDDDAIELIC
GEYDVEVEKEIILEDTDLDKYIEEDAEENLQERPAVVTIMGHVDHGKTTLLDSIRHTKVTAGEAGGITQHIGAYQVENDG
KKITFLDTPGHAAFTSMRSRGAQVTDIAILVVAADDGVMPQTVEAINHAKAAEVPIIVAVNKMDKEGANPDRVMQELTEH
QLIPEDWGGNTIFVNLSAIKNEGIDDLLEMILLVSEVEELKANPNAKAFGSVIDAQLDKGRGSVATLLVQNGTLHVGDPL
VVGSTFGKVRAMVNDLGNRVTEVGPSTPVEITGLHGVPQAGDQFLVFKDEKKARQIGEAREQKQIDENRGTQSTVSLDDL
FEQIKQGEMKELNIIVKADVQGSVEALAASLQKIEVEGVNVKIIHTGVGAITESDIILASASKAIVIGFNVRPDVNAKNA
AESEKVDLRLHRVIYNAIEEIESAMKGLLDPEYQEKVIGQAEVREIFKVSRIGTIAGSYVTDGKITRDAGVRLIRDGVVL
YEGELQALKRFKDDVKEVQTNYECGITISNFNDIKEGDTIEAFVMEEIERK

Specific function: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 7

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family

Homologues:

Organism=Homo sapiens, GI53729339, Length=665, Percent_Identity=36.8421052631579, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI53729337, Length=665, Percent_Identity=36.8421052631579, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI84043963, Length=469, Percent_Identity=27.5053304904051, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI157426893, Length=227, Percent_Identity=27.3127753303965, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI18390331, Length=143, Percent_Identity=33.5664335664336, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1789559, Length=631, Percent_Identity=51.188589540412, Blast_Score=606, Evalue=1e-174,
Organism=Escherichia coli, GI2367247, Length=240, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788922, Length=232, Percent_Identity=31.0344827586207, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI48994988, Length=158, Percent_Identity=32.9113924050633, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1790412, Length=236, Percent_Identity=32.6271186440678, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1789737, Length=236, Percent_Identity=32.6271186440678, Blast_Score=70, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71994658, Length=553, Percent_Identity=35.9855334538879, Blast_Score=315, Evalue=4e-86,
Organism=Caenorhabditis elegans, GI212656558, Length=458, Percent_Identity=26.6375545851528, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17557151, Length=246, Percent_Identity=29.2682926829268, Blast_Score=86, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=252, Percent_Identity=33.3333333333333, Blast_Score=76, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6324550, Length=635, Percent_Identity=35.5905511811024, Blast_Score=333, Evalue=5e-92,
Organism=Saccharomyces cerevisiae, GI6319282, Length=482, Percent_Identity=25.7261410788382, Blast_Score=125, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6324761, Length=245, Percent_Identity=31.4285714285714, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI28572034, Length=555, Percent_Identity=38.7387387387387, Blast_Score=342, Evalue=4e-94,
Organism=Drosophila melanogaster, GI24656849, Length=466, Percent_Identity=26.6094420600858, Blast_Score=121, Evalue=2e-27,
Organism=Drosophila melanogaster, GI160714833, Length=96, Percent_Identity=62.5, Blast_Score=117, Evalue=3e-26,
Organism=Drosophila melanogaster, GI78706572, Length=223, Percent_Identity=29.5964125560538, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI281363316, Length=257, Percent_Identity=31.5175097276265, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI17864358, Length=257, Percent_Identity=31.5175097276265, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): IF2_OCEIH (Q8EQU1)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692519.1
- ProteinModelPortal:   Q8EQU1
- SMR:   Q8EQU1
- GeneID:   1017986
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1598
- NMPDR:   fig|221109.1.peg.1600
- HOGENOM:   HBG635083
- OMA:   VRKKRTV
- ProtClustDB:   PRK05306
- BioCyc:   OIHE221109:OB1598-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00100_B
- InterPro:   IPR011989
- InterPro:   IPR006847
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000178
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:1.25.10.10
- Gene3D:   G3DSA:3.40.50.10050
- PANTHER:   PTHR23115:SF41
- TIGRFAMs:   TIGR00487
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; PF04760 IF2_N; SSF52156 TIF_IF2_dom3; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 76148; Mature: 76017

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS01176 IF2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIRIYEYAKQNNLASKDVINFLKTENVEVSNHMSAISDEVVKKLDNKFKAKPENAKKG
CCCEEEEEHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCC
QNQKQSNNQQQNRQKQNQKNQSKPNKNKKQKGPKKNQQQERPAAKPAETPGKITYHGTLT
CCHHHCCHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHCCCCCCCCCCCEEEEEEEEE
VQDLAGKLNKEPAELIKKLMFLGVMATKNQDIDDDAIELICGEYDVEVEKEIILEDTDLD
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHEECCCCCCCCCEEEEECCCHH
KYIEEDAEENLQERPAVVTIMGHVDHGKTTLLDSIRHTKVTAGEAGGITQHIGAYQVEND
HHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCEECCCCCCCCHHHCCCEEEECC
GKKITFLDTPGHAAFTSMRSRGAQVTDIAILVVAADDGVMPQTVEAINHAKAAEVPIIVA
CCEEEEEECCCHHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHHHHHHCCHHCCCCEEEE
VNKMDKEGANPDRVMQELTEHQLIPEDWGGNTIFVNLSAIKNEGIDDLLEMILLVSEVEE
EECCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHH
LKANPNAKAFGSVIDAQLDKGRGSVATLLVQNGTLHVGDPLVVGSTFGKVRAMVNDLGNR
HHCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCEEEECCCEEECCHHHHHHHHHHHHHHH
VTEVGPSTPVEITGLHGVPQAGDQFLVFKDEKKARQIGEAREQKQIDENRGTQSTVSLDD
HHHCCCCCCEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEECHHH
LFEQIKQGEMKELNIIVKADVQGSVEALAASLQKIEVEGVNVKIIHTGVGAITESDIILA
HHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHEEECCCEEEEEEECCCCCCCCCEEEE
SASKAIVIGFNVRPDVNAKNAAESEKVDLRLHRVIYNAIEEIESAMKGLLDPEYQEKVIG
ECCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCC
QAEVREIFKVSRIGTIAGSYVTDGKITRDAGVRLIRDGVVLYEGELQALKRFKDDVKEVQ
HHHHHHHHHHHHHHHHCCCEECCCEEEHHHCCEEEECCEEEECCHHHHHHHHHHHHHHHH
TNYECGITISNFNDIKEGDTIEAFVMEEIERK
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SKIRIYEYAKQNNLASKDVINFLKTENVEVSNHMSAISDEVVKKLDNKFKAKPENAKKG
CCEEEEEHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCC
QNQKQSNNQQQNRQKQNQKNQSKPNKNKKQKGPKKNQQQERPAAKPAETPGKITYHGTLT
CCHHHCCHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHCCCCCCCCCCCEEEEEEEEE
VQDLAGKLNKEPAELIKKLMFLGVMATKNQDIDDDAIELICGEYDVEVEKEIILEDTDLD
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHEECCCCCCCCCEEEEECCCHH
KYIEEDAEENLQERPAVVTIMGHVDHGKTTLLDSIRHTKVTAGEAGGITQHIGAYQVEND
HHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCEECCCCCCCCHHHCCCEEEECC
GKKITFLDTPGHAAFTSMRSRGAQVTDIAILVVAADDGVMPQTVEAINHAKAAEVPIIVA
CCEEEEEECCCHHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHHHHHHCCHHCCCCEEEE
VNKMDKEGANPDRVMQELTEHQLIPEDWGGNTIFVNLSAIKNEGIDDLLEMILLVSEVEE
EECCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHH
LKANPNAKAFGSVIDAQLDKGRGSVATLLVQNGTLHVGDPLVVGSTFGKVRAMVNDLGNR
HHCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCEEEECCCEEECCHHHHHHHHHHHHHHH
VTEVGPSTPVEITGLHGVPQAGDQFLVFKDEKKARQIGEAREQKQIDENRGTQSTVSLDD
HHHCCCCCCEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEECHHH
LFEQIKQGEMKELNIIVKADVQGSVEALAASLQKIEVEGVNVKIIHTGVGAITESDIILA
HHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHEEECCCEEEEEEECCCCCCCCCEEEE
SASKAIVIGFNVRPDVNAKNAAESEKVDLRLHRVIYNAIEEIESAMKGLLDPEYQEKVIG
ECCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCC
QAEVREIFKVSRIGTIAGSYVTDGKITRDAGVRLIRDGVVLYEGELQALKRFKDDVKEVQ
HHHHHHHHHHHHHHHHCCCEECCCEEEHHHCCEEEECCEEEECCHHHHHHHHHHHHHHHH
TNYECGITISNFNDIKEGDTIEAFVMEEIERK
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376