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The map label for this gene is nusA [H]

Identifier: 23099050

GI number: 23099050

Start: 1634394

End: 1635488

Strand: Direct

Name: nusA [H]

Synonym: OB1595

Alternate gene names: 23099050

Gene position: 1634394-1635488 (Clockwise)

Preceding gene: 23099049

Following gene: 23099051

Centisome position: 45.02

GC content: 37.63

Gene sequence:

>1095_bases
GTGAGCATGCAGTTATTTGATGCGATTGAGAATTTAGCAAAAGAAAAGGGAATTGATAAGGATATCTTAATGGAGGCCTT
AGAAGCAGCTTTAATCTCAGCGTATAAGAAAAATTTCAAATCTGCTTCCAATGTAAGAGTAGAGTTGAATGAAGAAACAG
GAAAAATGGCAGTTTATGCACGTAAAACAGTGGTAGATGAAATAGAAGATGTACAGCAAGAAATTCCATTAGATGAAGCA
AAGAAAATTGATCCAAACTATGAAGTTGATGATGTGATCGAAGTAGAAGTAACACCGAAAGATTTTGGACGTATTGCTGC
ACAAGCTGCAAAACAAGTTGTGACTCAACGAGTTCGAGAAGCGGAGAGAGGCGTGATTTTCTCAGAATATGTTGATCGAG
AAGAAGATGTCATGACTGGTATAATACAACGAAAAGATCCACGCTTTGTTTACGTTAATTTAGGTAAAATTGAAGCAAAA
TTAGCTGAGTCTGAGCAGATGCCTACGGAAGATTATCATGTGCATGATCGACTAAAAGTGTTTGTTACGAAAGTAGAAAA
TACAAGTAAGGGTCCACAAATTTTTATTTCTAGATCACACCCTGGTTTGTTGAAAAGACTATTTGAAATGGAAGTACCTG
AAATTTATGATGGAACTGTAGAAATTAAGTCAGTAGCTCGTGAAGCAGGAGATCGCTCAAAAATTTCTGTGTATGCCCCA
GATCCTGAAATAGATCCAGTTGGTTCTTGTGTTGGACAACGTGGACAACGAGTGCAGGCAATAGTAACAGAACTCAAAGG
TGAGAAAATAGACATTGTTCAATGGTCAGAAGATCCAGTGGTATATGTATCTAATGCGTTGAGTCCATCTAAAGTGGTTA
AGGTTTCGGTAAACGAGGAAGAGAAATCAACGACAGTAGTCGTTCCAGATTACCAATTATCCCTAGCTATTGGTAAGAGA
GGCCAAAATGCCAGATTAGCTGCTAAACTAACAGGATGGAAGATAGATATTAAAAGTGAATCCGAAGCAAGAGAAGAAGG
ATTGCTAGACGATGGTGAATCTTATTCTGACAATGAAAACGACTTGTTTGAATAA

Upstream 100 bases:

>100_bases
TAAAGTGAAAACGCGTAAGAAGCAAGTAGAGATACCTTATAGTAAAATTGCCAAAGCAAGAATTGCTGTTACGTTTTAAT
ACAAAGGGGGAAAAGAAAAA

Downstream 100 bases:

>100_bases
GGAGGTAAATGTCATTGGTTAAGAAGAAAAAAATACCAGAGCGTAAATGTATAGTCACCAATGAAATGAGACCGAAGAAA
GAATTAATTCGTGTCGTGCG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MSMQLFDAIENLAKEKGIDKDILMEALEAALISAYKKNFKSASNVRVELNEETGKMAVYARKTVVDEIEDVQQEIPLDEA
KKIDPNYEVDDVIEVEVTPKDFGRIAAQAAKQVVTQRVREAERGVIFSEYVDREEDVMTGIIQRKDPRFVYVNLGKIEAK
LAESEQMPTEDYHVHDRLKVFVTKVENTSKGPQIFISRSHPGLLKRLFEMEVPEIYDGTVEIKSVAREAGDRSKISVYAP
DPEIDPVGSCVGQRGQRVQAIVTELKGEKIDIVQWSEDPVVYVSNALSPSKVVKVSVNEEEKSTTVVVPDYQLSLAIGKR
GQNARLAAKLTGWKIDIKSESEAREEGLLDDGESYSDNENDLFE

Sequences:

>Translated_364_residues
MSMQLFDAIENLAKEKGIDKDILMEALEAALISAYKKNFKSASNVRVELNEETGKMAVYARKTVVDEIEDVQQEIPLDEA
KKIDPNYEVDDVIEVEVTPKDFGRIAAQAAKQVVTQRVREAERGVIFSEYVDREEDVMTGIIQRKDPRFVYVNLGKIEAK
LAESEQMPTEDYHVHDRLKVFVTKVENTSKGPQIFISRSHPGLLKRLFEMEVPEIYDGTVEIKSVAREAGDRSKISVYAP
DPEIDPVGSCVGQRGQRVQAIVTELKGEKIDIVQWSEDPVVYVSNALSPSKVVKVSVNEEEKSTTVVVPDYQLSLAIGKR
GQNARLAAKLTGWKIDIKSESEAREEGLLDDGESYSDNENDLFE
>Mature_363_residues
SMQLFDAIENLAKEKGIDKDILMEALEAALISAYKKNFKSASNVRVELNEETGKMAVYARKTVVDEIEDVQQEIPLDEAK
KIDPNYEVDDVIEVEVTPKDFGRIAAQAAKQVVTQRVREAERGVIFSEYVDREEDVMTGIIQRKDPRFVYVNLGKIEAKL
AESEQMPTEDYHVHDRLKVFVTKVENTSKGPQIFISRSHPGLLKRLFEMEVPEIYDGTVEIKSVAREAGDRSKISVYAPD
PEIDPVGSCVGQRGQRVQAIVTELKGEKIDIVQWSEDPVVYVSNALSPSKVVKVSVNEEEKSTTVVVPDYQLSLAIGKRG
QNARLAAKLTGWKIDIKSESEAREEGLLDDGESYSDNENDLFE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=369, Percent_Identity=36.0433604336043, Blast_Score=261, Evalue=4e-71,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 40857; Mature: 40725

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS50084 KH_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMQLFDAIENLAKEKGIDKDILMEALEAALISAYKKNFKSASNVRVELNEETGKMAVYA
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEE
RKTVVDEIEDVQQEIPLDEAKKIDPNYEVDDVIEVEVTPKDFGRIAAQAAKQVVTQRVRE
HHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
AERGVIFSEYVDREEDVMTGIIQRKDPRFVYVNLGKIEAKLAESEQMPTEDYHVHDRLKV
HHCCEEHHHHHCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCCCCCHHHEEEE
FVTKVENTSKGPQIFISRSHPGLLKRLFEMEVPEIYDGTVEIKSVAREAGDRSKISVYAP
EEEECCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEEECC
DPEIDPVGSCVGQRGQRVQAIVTELKGEKIDIVQWSEDPVVYVSNALSPSKVVKVSVNEE
CCCCCHHHHHHHHCCHHHHHHHHHHCCCEEEEEEECCCCEEEEECCCCCCCEEEEEECCC
EKSTTVVVPDYQLSLAIGKRGQNARLAAKLTGWKIDIKSESEAREEGLLDDGESYSDNEN
CCCCEEEECCCEEEEEECCCCCCCEEEEEEECEEEEECCCHHHHHCCCCCCCCCCCCCCC
DLFE
CCCC
>Mature Secondary Structure 
SMQLFDAIENLAKEKGIDKDILMEALEAALISAYKKNFKSASNVRVELNEETGKMAVYA
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEE
RKTVVDEIEDVQQEIPLDEAKKIDPNYEVDDVIEVEVTPKDFGRIAAQAAKQVVTQRVRE
HHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
AERGVIFSEYVDREEDVMTGIIQRKDPRFVYVNLGKIEAKLAESEQMPTEDYHVHDRLKV
HHCCEEHHHHHCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCCCCCHHHEEEE
FVTKVENTSKGPQIFISRSHPGLLKRLFEMEVPEIYDGTVEIKSVAREAGDRSKISVYAP
EEEECCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEEECC
DPEIDPVGSCVGQRGQRVQAIVTELKGEKIDIVQWSEDPVVYVSNALSPSKVVKVSVNEE
CCCCCHHHHHHHHCCHHHHHHHHHHCCCEEEEEEECCCCEEEEECCCCCCCEEEEEECCC
EKSTTVVVPDYQLSLAIGKRGQNARLAAKLTGWKIDIKSESEAREEGLLDDGESYSDNEN
CCCCEEEECCCEEEEEECCCCCCCEEEEEEECEEEEECCCHHHHHCCCCCCCCCCCCCCC
DLFE
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]