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The map label for this gene is pbpB [H]

Identifier: 23098919

GI number: 23098919

Start: 1500408

End: 1502648

Strand: Direct

Name: pbpB [H]

Synonym: OB1464

Alternate gene names: 23098919

Gene position: 1500408-1502648 (Clockwise)

Preceding gene: 23098918

Following gene: 23098920

Centisome position: 41.33

GC content: 37.66

Gene sequence:

>2241_bases
ATGAAAAAACATAGAACGACGCATTTTATGTCTAGTATATATATGATTATTTTCGTGGCATTATTTTTAATAATTACAGG
AAGATTCATGTATATCCAAGCAACTGGTGAAGTTAGTGGAGTTCAATTAAATGAATGGGCTGATAAACAACGAACTGCAT
CATACACCATGAATTCAGAAAGGGGAAAGATTTATGATAAGAATGGCATGGTCCTTGCCTATGACCGCCCAACTTATCGA
ATGTACGCGATCCTAGATGAAGCTTATTCTGAAAATGCTGAGGAGCCATTACATGTTTCAAACGCAGAAGAAACTGCTAA
GAAATTGGCTCCTCTTTTAGGTGTTGAGGAAGAGGAAATACAAGAACCTATACAGAACGGACTTGACAATAATCGATTTC
AAGTAGAATTTGGGAAGTTTGGAAAACAAATTTCCAAGCAAGTAAAAGATGAAATTGAAGATTTGAATATTCCAGGCATT
AATTTTGAGGAAGAACCGATGCGTTATTACCCTAATGGAATGTTTGCATCCCATATTATCGGTTTTGCACGAGAAGAAGA
AAAGGAAACGGATGAAGGTGTGGAACATACCATTACAGGAGTTACTGGAATGGAAGATGTCATGAATGACATCTTAGGCG
GCAAAGATGGTTATATTTCATATCAAAGAGATCTGTATAATAAAAAATTATTGGATCCAAGTGAAGTTATTCAGTTGCCA
GAAGACGGAGACGATATTTACTTAACGATAGATCAAAAGATACAAACTTTATTAGAAGATGTAATGACACAAGCAGATGA
GAAGTATAATCCGACTAAAATGAATGCGATTATAATGGATCCAAAATCAGGTGAAATTGTTGCAATGAGTTCTCGTCCAA
GCTATGATCCGAATAATCCAACCGATGTAGAAAATTGGTACAATGACCCAATATCTACTCCGTTCGAGCCAGGCTCTACG
ATGAAAATATTCACCTGGGCAGCAGCTATAGAAGAAGGAGTATATAATGGTTCTGAAGGATTTAAATCGGGAACATATCA
GCCGAATGAGAAAATAAGAGCAATTGGCGATCATAATAATGGTGAAGGATGGGGGACGATTTCTTATGATGAGGGATTTG
AACGTTCTTCGAATGTAGCAGCTTCGAAATTATTATGGGAAAAATTAGATTCAGAATCATATTATGAATATCTACAAGCA
TTTGGTTTTGAGGAACCAACTGGAATTGATCTCCCAGGGGAAGCTGTAGGGAAAATATCGTATAATTGGCCGTCAGATAA
GTTGAGAACGGCTTTTGGACAAAGTTCAACGGTAACCCCTATACAGCAAATGAAAGCAGCATCTGCGATTGTTAATGGTG
GGAAAATGGTTAAACCCTATGTAGTAGATAAAGTAGTAGATTCAACATCAGGAGATACTATCTCAGAAACAGAGCAAGAG
TATGTAGGTCAGCCAATATCTGAAGAAACGGCTGAACAGATGAAAAAATTAATGTCATCTGTGGTCAATGGGGAACACGG
AACAGGAAAACCGTATCAATTAGAAGATTATATGGTTGGTGGTAAAACTGGTACTGCAGAGATTCCAAATCCTGATGGTG
GCGGGTATCTCCAAGGGAGAGAAAATTATGTGTTTTCTTTCATGGGAATGGCGCCAATAGACGATCCTCAATTAATGGTA
TATGTATCAATTCAACAACCTGAGTTAGAGCCGGATGAGTCTGGTTCAACACCACTCTCGTTTATATTTAAAAATGTAGT
AGAGAATAGTTTGCATTACATGGATATTAATCCTGAGAAGAATGAAGCTCCAGATATACAACAGATGGAGATGCCAGATG
TTGTTAATAAATCGACTTCAGAAGTAGAAGAAGAAATGACAGAAACAGGATTAAATACGATTGTGGTTGGGGAAGGTTCT
AAAGTGGTCTCCAGCAGTATTGAAGCTGGGGCATCTGTTCTTCCAAATGAAAAAGTTATACTGATAACTGATGAACCGAC
TATGCCGGACATTAAAGGCTGGTCTCTTCGTGAGGTAATGACCTTAGCTAATTTATTAGATTTACAAGTAGAAAGTTTTG
GTTCAGGCTATGTTACGACTCAAAATATTGATGTTGGAAATCCGTTGCAATCTGGAGATTATTTAGGAGTTGAACTAGAG
GGACCGAGCTCTACCGATGACGAGGAAGAATCTTCAGAAGAAGAAAATGAAGAAGAAACAGGAGAAGACGAATCTGAGTA
A

Upstream 100 bases:

>100_bases
GAACGTATAATTAATATTGCTGAAGAGAACGGTCTAAAAATTCAAAATACAGAAGTGAAGCAAGTACAATCGTTCAACAA
CTAGGCAAGGGGAATACAAA

Downstream 100 bases:

>100_bases
ACGATGGAAAGGACAAACAGTGTTCTATTCAAAATTCTTTATTCATATAGTGGATACAAAGTAGCCCAAGAAGGAGTTGG
CTTATGAAACGTGTATCTAC

Product: penicillin-binding protein 2B

Products: NA

Alternate protein names: PBP-2B; Penicillin-binding protein B [H]

Number of amino acids: Translated: 746; Mature: 746

Protein sequence:

>746_residues
MKKHRTTHFMSSIYMIIFVALFLIITGRFMYIQATGEVSGVQLNEWADKQRTASYTMNSERGKIYDKNGMVLAYDRPTYR
MYAILDEAYSENAEEPLHVSNAEETAKKLAPLLGVEEEEIQEPIQNGLDNNRFQVEFGKFGKQISKQVKDEIEDLNIPGI
NFEEEPMRYYPNGMFASHIIGFAREEEKETDEGVEHTITGVTGMEDVMNDILGGKDGYISYQRDLYNKKLLDPSEVIQLP
EDGDDIYLTIDQKIQTLLEDVMTQADEKYNPTKMNAIIMDPKSGEIVAMSSRPSYDPNNPTDVENWYNDPISTPFEPGST
MKIFTWAAAIEEGVYNGSEGFKSGTYQPNEKIRAIGDHNNGEGWGTISYDEGFERSSNVAASKLLWEKLDSESYYEYLQA
FGFEEPTGIDLPGEAVGKISYNWPSDKLRTAFGQSSTVTPIQQMKAASAIVNGGKMVKPYVVDKVVDSTSGDTISETEQE
YVGQPISEETAEQMKKLMSSVVNGEHGTGKPYQLEDYMVGGKTGTAEIPNPDGGGYLQGRENYVFSFMGMAPIDDPQLMV
YVSIQQPELEPDESGSTPLSFIFKNVVENSLHYMDINPEKNEAPDIQQMEMPDVVNKSTSEVEEEMTETGLNTIVVGEGS
KVVSSSIEAGASVLPNEKVILITDEPTMPDIKGWSLREVMTLANLLDLQVESFGSGYVTTQNIDVGNPLQSGDYLGVELE
GPSSTDDEEESSEEENEEETGEDESE

Sequences:

>Translated_746_residues
MKKHRTTHFMSSIYMIIFVALFLIITGRFMYIQATGEVSGVQLNEWADKQRTASYTMNSERGKIYDKNGMVLAYDRPTYR
MYAILDEAYSENAEEPLHVSNAEETAKKLAPLLGVEEEEIQEPIQNGLDNNRFQVEFGKFGKQISKQVKDEIEDLNIPGI
NFEEEPMRYYPNGMFASHIIGFAREEEKETDEGVEHTITGVTGMEDVMNDILGGKDGYISYQRDLYNKKLLDPSEVIQLP
EDGDDIYLTIDQKIQTLLEDVMTQADEKYNPTKMNAIIMDPKSGEIVAMSSRPSYDPNNPTDVENWYNDPISTPFEPGST
MKIFTWAAAIEEGVYNGSEGFKSGTYQPNEKIRAIGDHNNGEGWGTISYDEGFERSSNVAASKLLWEKLDSESYYEYLQA
FGFEEPTGIDLPGEAVGKISYNWPSDKLRTAFGQSSTVTPIQQMKAASAIVNGGKMVKPYVVDKVVDSTSGDTISETEQE
YVGQPISEETAEQMKKLMSSVVNGEHGTGKPYQLEDYMVGGKTGTAEIPNPDGGGYLQGRENYVFSFMGMAPIDDPQLMV
YVSIQQPELEPDESGSTPLSFIFKNVVENSLHYMDINPEKNEAPDIQQMEMPDVVNKSTSEVEEEMTETGLNTIVVGEGS
KVVSSSIEAGASVLPNEKVILITDEPTMPDIKGWSLREVMTLANLLDLQVESFGSGYVTTQNIDVGNPLQSGDYLGVELE
GPSSTDDEEESSEEENEEETGEDESE
>Mature_746_residues
MKKHRTTHFMSSIYMIIFVALFLIITGRFMYIQATGEVSGVQLNEWADKQRTASYTMNSERGKIYDKNGMVLAYDRPTYR
MYAILDEAYSENAEEPLHVSNAEETAKKLAPLLGVEEEEIQEPIQNGLDNNRFQVEFGKFGKQISKQVKDEIEDLNIPGI
NFEEEPMRYYPNGMFASHIIGFAREEEKETDEGVEHTITGVTGMEDVMNDILGGKDGYISYQRDLYNKKLLDPSEVIQLP
EDGDDIYLTIDQKIQTLLEDVMTQADEKYNPTKMNAIIMDPKSGEIVAMSSRPSYDPNNPTDVENWYNDPISTPFEPGST
MKIFTWAAAIEEGVYNGSEGFKSGTYQPNEKIRAIGDHNNGEGWGTISYDEGFERSSNVAASKLLWEKLDSESYYEYLQA
FGFEEPTGIDLPGEAVGKISYNWPSDKLRTAFGQSSTVTPIQQMKAASAIVNGGKMVKPYVVDKVVDSTSGDTISETEQE
YVGQPISEETAEQMKKLMSSVVNGEHGTGKPYQLEDYMVGGKTGTAEIPNPDGGGYLQGRENYVFSFMGMAPIDDPQLMV
YVSIQQPELEPDESGSTPLSFIFKNVVENSLHYMDINPEKNEAPDIQQMEMPDVVNKSTSEVEEEMTETGLNTIVVGEGS
KVVSSSIEAGASVLPNEKVILITDEPTMPDIKGWSLREVMTLANLLDLQVESFGSGYVTTQNIDVGNPLQSGDYLGVELE
GPSSTDDEEESSEEENEEETGEDESE

Specific function: Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. PBP-2B is required for vegetative cell division and sporulation septation. Beta-lactamase inactivates the PBPs by acylating an essential serine residue in the active sit

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PASTA domains [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=603, Percent_Identity=24.8756218905473, Blast_Score=151, Evalue=2e-37,
Organism=Escherichia coli, GI1786854, Length=563, Percent_Identity=23.8010657193606, Blast_Score=128, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005543
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03793 PASTA; PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 83053; Mature: 83053

Theoretical pI: Translated: 3.98; Mature: 3.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKHRTTHFMSSIYMIIFVALFLIITGRFMYIQATGEVSGVQLNEWADKQRTASYTMNSE
CCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEHHHHCHHHHHHEEECCC
RGKIYDKNGMVLAYDRPTYRMYAILDEAYSENAEEPLHVSNAEETAKKLAPLLGVEEEEI
CCEEEECCCEEEEECCCCEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHH
QEPIQNGLDNNRFQVEFGKFGKQISKQVKDEIEDLNIPGINFEEEPMRYYPNGMFASHII
HHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCHHHHHHH
GFAREEEKETDEGVEHTITGVTGMEDVMNDILGGKDGYISYQRDLYNKKLLDPSEVIQLP
HHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHCCCCCCEEHHHHHHHHCCCCHHHHEECC
EDGDDIYLTIDQKIQTLLEDVMTQADEKYNPTKMNAIIMDPKSGEIVAMSSRPSYDPNNP
CCCCEEEEEECHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCC
TDVENWYNDPISTPFEPGSTMKIFTWAAAIEEGVYNGSEGFKSGTYQPNEKIRAIGDHNN
CCCHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCC
GEGWGTISYDEGFERSSNVAASKLLWEKLDSESYYEYLQAFGFEEPTGIDLPGEAVGKIS
CCCCEEECCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHCEEE
YNWPSDKLRTAFGQSSTVTPIQQMKAASAIVNGGKMVKPYVVDKVVDSTSGDTISETEQE
ECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEECHHHHHHHHHCCCCCCHHHHHHH
YVGQPISEETAEQMKKLMSSVVNGEHGTGKPYQLEDYMVGGKTGTAEIPNPDGGGYLQGR
HCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCEECCC
ENYVFSFMGMAPIDDPQLMVYVSIQQPELEPDESGSTPLSFIFKNVVENSLHYMDINPEK
CCEEEEEECCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCC
NEAPDIQQMEMPDVVNKSTSEVEEEMTETGLNTIVVGEGSKVVSSSIEAGASVLPNEKVI
CCCCCCHHCCCCHHHCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCHHCCCCCCEE
LITDEPTMPDIKGWSLREVMTLANLLDLQVESFGSGYVTTQNIDVGNPLQSGDYLGVELE
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCEEEEEEC
GPSSTDDEEESSEEENEEETGEDESE
CCCCCCCCHHHHHHHCHHHCCCCCCC
>Mature Secondary Structure
MKKHRTTHFMSSIYMIIFVALFLIITGRFMYIQATGEVSGVQLNEWADKQRTASYTMNSE
CCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEHHHHCHHHHHHEEECCC
RGKIYDKNGMVLAYDRPTYRMYAILDEAYSENAEEPLHVSNAEETAKKLAPLLGVEEEEI
CCEEEECCCEEEEECCCCEEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHH
QEPIQNGLDNNRFQVEFGKFGKQISKQVKDEIEDLNIPGINFEEEPMRYYPNGMFASHII
HHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCHHHHHHH
GFAREEEKETDEGVEHTITGVTGMEDVMNDILGGKDGYISYQRDLYNKKLLDPSEVIQLP
HHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHCCCCCCEEHHHHHHHHCCCCHHHHEECC
EDGDDIYLTIDQKIQTLLEDVMTQADEKYNPTKMNAIIMDPKSGEIVAMSSRPSYDPNNP
CCCCEEEEEECHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCC
TDVENWYNDPISTPFEPGSTMKIFTWAAAIEEGVYNGSEGFKSGTYQPNEKIRAIGDHNN
CCCHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCC
GEGWGTISYDEGFERSSNVAASKLLWEKLDSESYYEYLQAFGFEEPTGIDLPGEAVGKIS
CCCCEEECCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHCEEE
YNWPSDKLRTAFGQSSTVTPIQQMKAASAIVNGGKMVKPYVVDKVVDSTSGDTISETEQE
ECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEECHHHHHHHHHCCCCCCHHHHHHH
YVGQPISEETAEQMKKLMSSVVNGEHGTGKPYQLEDYMVGGKTGTAEIPNPDGGGYLQGR
HCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCEECCC
ENYVFSFMGMAPIDDPQLMVYVSIQQPELEPDESGSTPLSFIFKNVVENSLHYMDINPEK
CCEEEEEECCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCC
NEAPDIQQMEMPDVVNKSTSEVEEEMTETGLNTIVVGEGSKVVSSSIEAGASVLPNEKVI
CCCCCCHHCCCCHHHCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCHHCCCCCCEE
LITDEPTMPDIKGWSLREVMTLANLLDLQVESFGSGYVTTQNIDVGNPLQSGDYLGVELE
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCEEEEEEC
GPSSTDDEEESSEEENEEETGEDESE
CCCCCCCCHHHHHHHCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8244929; 9384377; 8289242 [H]