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The map label for this gene is dacB [H]

Identifier: 23098797

GI number: 23098797

Start: 1385061

End: 1386242

Strand: Direct

Name: dacB [H]

Synonym: OB1342

Alternate gene names: 23098797

Gene position: 1385061-1386242 (Clockwise)

Preceding gene: 23098794

Following gene: 23098798

Centisome position: 38.15

GC content: 33.5

Gene sequence:

>1182_bases
ATGCTGAGGAAATTTTACATCATGATTATAATAACATCACTTAATTTTTTTATATGGACAAGTACTACGTTAGCAGATGA
AGACCTTTCGTTACATAGTGAATCAGCTATTTTAATGGAAGCGGAATCTGGACAAATTCTTGCAGAGAAAAACGCAAATC
AGCAAATGTATCCGGCGAGTGTAACAAAAATAGCAACTGCGATTTATGTAATAGAAAATGGTAATCTATCTGAAGAAGTA
GAAATAAGTGAAAAAGCTACAGAAGCAGATGGTACTACTGTATTCTTAGCAGAGGGAGAAAAGGTCTCTCTCGGTAAACT
TGTTAAAGGTTTATTAATAAATTCAGGAAATGATGCAGGTGTAGCGATCGCAGAACATATGAATGGCAGTGTTCGTGAAT
TCTCAAAAGAAATGACCGAGTTTTTTGAGGAAAAAGCTGGGATAGAAAATACGAATTTTGATAATCCGCATGGTTTATTT
GATGATAATCATTATACAACGGCTGAGGATCTTGCAAAAATAACGAAATATGCTATGCAAAATGAAAAATTTGTAAAGTT
ATTTGGAATGAAATCCGTGGATTGGGAAAGTAAAACATGGGAAACAACGATTTTAAATCATCATCGAATGGTGAAAGGTG
AAATACCTTATGATGGAGTAACCGGAGGAAAAAATGGATATGTGTCCCGGTCTGGTTTTACTCTTGTTACAACTGCAGAA
CAAGACGGGATGAATTTAATTGCTGTAACTTTGAAGACGCCACTTTCTGAACAATCTTATGAAGATACAGTTGAGTTATT
GGATTATGGGTTTGAACATTACGAACCATTTACAATTGATGAAGGCACTTCCTTTATGACAAATGGCAAGCAATTTAAAA
ATAGTGAAGATTTAACTTATGTTCATGAAGAAGGATCAAAAGTAGAAGAAGAAATTAAAGAAGATGGTAAACTTGTAATT
GAGGATAACGGAGAATTACTGAAGGAATTCCAACTGGATAATGTTGAAAAGAAACAACCGACAATGATGAAAAACAATCA
AGTTGAGAACGAAAATCAAGAAGATAGTCAAAGCTCTGTATATCTCTATATTGGAATTGGAGTAGTTGCAGCAATATTAG
TGCTCGTATTAATTTTATTATGGAGAAGAAAGAAGAGACGAAAGAATAATATATTCATATAA

Upstream 100 bases:

>100_bases
TGTAATATTATAGGTTGTTTACAATTTTTATTCATATATTTACATGAATATACATGTCTATTATTCTTATTTATGGAATG
AATTGAAGGAGTCGTTGGAT

Downstream 100 bases:

>100_bases
TGCAATAAGAAACCTTAAGGTGTTCGACTAAACATCCTTAAGGTTTTTTTCATAACAAAACAAATAATTGGCTGTAATAT
ACAGGTTACAAGGCATAGTG

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MLRKFYIMIIITSLNFFIWTSTTLADEDLSLHSESAILMEAESGQILAEKNANQQMYPASVTKIATAIYVIENGNLSEEV
EISEKATEADGTTVFLAEGEKVSLGKLVKGLLINSGNDAGVAIAEHMNGSVREFSKEMTEFFEEKAGIENTNFDNPHGLF
DDNHYTTAEDLAKITKYAMQNEKFVKLFGMKSVDWESKTWETTILNHHRMVKGEIPYDGVTGGKNGYVSRSGFTLVTTAE
QDGMNLIAVTLKTPLSEQSYEDTVELLDYGFEHYEPFTIDEGTSFMTNGKQFKNSEDLTYVHEEGSKVEEEIKEDGKLVI
EDNGELLKEFQLDNVEKKQPTMMKNNQVENENQEDSQSSVYLYIGIGVVAAILVLVLILLWRRKKRRKNNIFI

Sequences:

>Translated_393_residues
MLRKFYIMIIITSLNFFIWTSTTLADEDLSLHSESAILMEAESGQILAEKNANQQMYPASVTKIATAIYVIENGNLSEEV
EISEKATEADGTTVFLAEGEKVSLGKLVKGLLINSGNDAGVAIAEHMNGSVREFSKEMTEFFEEKAGIENTNFDNPHGLF
DDNHYTTAEDLAKITKYAMQNEKFVKLFGMKSVDWESKTWETTILNHHRMVKGEIPYDGVTGGKNGYVSRSGFTLVTTAE
QDGMNLIAVTLKTPLSEQSYEDTVELLDYGFEHYEPFTIDEGTSFMTNGKQFKNSEDLTYVHEEGSKVEEEIKEDGKLVI
EDNGELLKEFQLDNVEKKQPTMMKNNQVENENQEDSQSSVYLYIGIGVVAAILVLVLILLWRRKKRRKNNIFI
>Mature_393_residues
MLRKFYIMIIITSLNFFIWTSTTLADEDLSLHSESAILMEAESGQILAEKNANQQMYPASVTKIATAIYVIENGNLSEEV
EISEKATEADGTTVFLAEGEKVSLGKLVKGLLINSGNDAGVAIAEHMNGSVREFSKEMTEFFEEKAGIENTNFDNPHGLF
DDNHYTTAEDLAKITKYAMQNEKFVKLFGMKSVDWESKTWETTILNHHRMVKGEIPYDGVTGGKNGYVSRSGFTLVTTAE
QDGMNLIAVTLKTPLSEQSYEDTVELLDYGFEHYEPFTIDEGTSFMTNGKQFKNSEDLTYVHEEGSKVEEEIKEDGKLVI
EDNGELLKEFQLDNVEKKQPTMMKNNQVENENQEDSQSSVYLYIGIGVVAAILVLVLILLWRRKKRRKNNIFI

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=319, Percent_Identity=28.5266457680251, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI87082022, Length=295, Percent_Identity=30.1694915254237, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1786851, Length=267, Percent_Identity=29.9625468164794, Blast_Score=102, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 44337; Mature: 44337

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRKFYIMIIITSLNFFIWTSTTLADEDLSLHSESAILMEAESGQILAEKNANQQMYPAS
CCCCEEEEEEEECCCEEEEECCEECCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCHH
VTKIATAIYVIENGNLSEEVEISEKATEADGTTVFLAEGEKVSLGKLVKGLLINSGNDAG
HHEEEEEEEEEECCCCCCEEEHHHHCCCCCCCEEEEECCCEECHHHHHHHHEEECCCCCC
VAIAEHMNGSVREFSKEMTEFFEEKAGIENTNFDNPHGLFDDNHYTTAEDLAKITKYAMQ
EEEHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
NEKFVKLFGMKSVDWESKTWETTILNHHRMVKGEIPYDGVTGGKNGYVSRSGFTLVTTAE
CCCEEEEECCCCCCCCCCCEEEHHHCCCEEEECCCCCCCCCCCCCCEEECCCEEEEEECC
QDGMNLIAVTLKTPLSEQSYEDTVELLDYGFEHYEPFTIDEGTSFMTNGKQFKNSEDLTY
CCCCEEEEEEEECCCCCCHHHHHHHHHHCCHHHCCCEEECCCCCCCCCCHHCCCCCCCEE
VHEEGSKVEEEIKEDGKLVIEDNGELLKEFQLDNVEKKQPTMMKNNQVENENQEDSQSSV
EECCCCHHHHHHHCCCCEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
YLYIGIGVVAAILVLVLILLWRRKKRRKNNIFI
EEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLRKFYIMIIITSLNFFIWTSTTLADEDLSLHSESAILMEAESGQILAEKNANQQMYPAS
CCCCEEEEEEEECCCEEEEECCEECCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCHH
VTKIATAIYVIENGNLSEEVEISEKATEADGTTVFLAEGEKVSLGKLVKGLLINSGNDAG
HHEEEEEEEEEECCCCCCEEEHHHHCCCCCCCEEEEECCCEECHHHHHHHHEEECCCCCC
VAIAEHMNGSVREFSKEMTEFFEEKAGIENTNFDNPHGLFDDNHYTTAEDLAKITKYAMQ
EEEHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
NEKFVKLFGMKSVDWESKTWETTILNHHRMVKGEIPYDGVTGGKNGYVSRSGFTLVTTAE
CCCEEEEECCCCCCCCCCCEEEHHHCCCEEEECCCCCCCCCCCCCCEEECCCEEEEEECC
QDGMNLIAVTLKTPLSEQSYEDTVELLDYGFEHYEPFTIDEGTSFMTNGKQFKNSEDLTY
CCCCEEEEEEEECCCCCCHHHHHHHHHHCCHHHCCCEEECCCCCCCCCCHHCCCCCCCEE
VHEEGSKVEEEIKEDGKLVIEDNGELLKEFQLDNVEKKQPTMMKNNQVENENQEDSQSSV
EECCCCHHHHHHHCCCCEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
YLYIGIGVVAAILVLVLILLWRRKKRRKNNIFI
EEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]