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The map label for this gene is ddl

Identifier: 23098761

GI number: 23098761

Start: 1350503

End: 1351603

Strand: Direct

Name: ddl

Synonym: OB1306

Alternate gene names: 23098761

Gene position: 1350503-1351603 (Clockwise)

Preceding gene: 23098760

Following gene: 23098762

Centisome position: 37.2

GC content: 35.97

Gene sequence:

>1101_bases
ATGAAGAAAAAAGTTGGTATTGTATTTGGAGGTAAGTCAGCAGAACATGAGGTTTCACTGCAGTCTGCTAAAAACATTGT
AGATGCAATCGACTCAGATAAATACGACGTATACTTATTAGGAATTGATAAGCAAGGGAATTGGCATGTCAATGATCGAT
CGCATTACTTGATAAATGAAGAGGATCCGAAATTAATTTCATTAAACAAAACAAATGAAGGAATTGCTGTTATTCCTGGA
GCAGAAAAAAACCAGATCGTTACTTCGAATTCAACTAAATCTCTTGATCAGTTAGATGTAATTTTTCCAATTGTTCATGG
GACGCTCGGAGAAGATGGAAGCTTACAAGGGATGCTTCGAATGGCAAACATTCCATTTGTCGGATCTAGTGTATTGGGCT
CTGCTATTAGCATGGATAAAGACATTGCCAAACGTCTATTGAAAGACGCTGGATTAAAAGTAGCAAAAGGATATGCTTAT
CGAAGTGTGGACAAGGAAAGCATTCATTTTGATAGTCTTAAAGAAGAATTAGGATTACCTATGTTTATTAAACCAGCAAA
CCAGGGTTCGTCGGTTGGTGTTCACAAAGTAGAAAACGAAGAACAATTTTATTCGGCTATTAAGGATGCATTTCAATTTG
ATCATAAATTACTCGTTGAAGAAGCAATTGTAGGAAGGGAAATTGAATGTGCTGTTCTGGGAAATGAAAAACCAGCTGCA
TCTGTTCCTGGAGAAATTTTACCGACGGGAGATTTTTACTCCTATGAAGCGAAATATATTGATGAAACGGGGGCAGTACT
TCAAATTCCTGCTAAACTAGAAGAACCTGTAGTAGACAGTATACAAGATATTGCTTTACAAGCTTTTAAGGCATTGAATT
GTGAAGGTCTGGCAAGAGTTGATGTGTTTTTAACGGATGACAACGAAATTGTAATCAATGAAATAAATACACTTCCAGGT
TTTACAAAAATAAGCATGTATCCAAAATTATGGGAAGAAAGCGGAGTATCTTATCGTGAATTAATCGAGAATTTAATAGA
ACTTGCTATAGAGAGAAATCAACGTGACCAACAATTAAAGAGTTCTAGAAGTGATGGATAG

Upstream 100 bases:

>100_bases
TAAAATATACGATACAATGCAAGTTAATTTTTACATTACATAATATGAAATTAGAGAAATATGTATGAAATAATCGTTGG
TTTTGTTAGGAGAGGATAAC

Downstream 100 bases:

>100_bases
GGGCTAATTACTTAGTCCCTTTTTAATGCTAGATTAGTATATAGTTTTGCTTTAATATAGGTATTAACGTTTAAAGAATT
GAGGGAGTTTGGAATGAATG

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKKKVGIVFGGKSAEHEVSLQSAKNIVDAIDSDKYDVYLLGIDKQGNWHVNDRSHYLINEEDPKLISLNKTNEGIAVIPG
AEKNQIVTSNSTKSLDQLDVIFPIVHGTLGEDGSLQGMLRMANIPFVGSSVLGSAISMDKDIAKRLLKDAGLKVAKGYAY
RSVDKESIHFDSLKEELGLPMFIKPANQGSSVGVHKVENEEQFYSAIKDAFQFDHKLLVEEAIVGREIECAVLGNEKPAA
SVPGEILPTGDFYSYEAKYIDETGAVLQIPAKLEEPVVDSIQDIALQAFKALNCEGLARVDVFLTDDNEIVINEINTLPG
FTKISMYPKLWEESGVSYRELIENLIELAIERNQRDQQLKSSRSDG

Sequences:

>Translated_366_residues
MKKKVGIVFGGKSAEHEVSLQSAKNIVDAIDSDKYDVYLLGIDKQGNWHVNDRSHYLINEEDPKLISLNKTNEGIAVIPG
AEKNQIVTSNSTKSLDQLDVIFPIVHGTLGEDGSLQGMLRMANIPFVGSSVLGSAISMDKDIAKRLLKDAGLKVAKGYAY
RSVDKESIHFDSLKEELGLPMFIKPANQGSSVGVHKVENEEQFYSAIKDAFQFDHKLLVEEAIVGREIECAVLGNEKPAA
SVPGEILPTGDFYSYEAKYIDETGAVLQIPAKLEEPVVDSIQDIALQAFKALNCEGLARVDVFLTDDNEIVINEINTLPG
FTKISMYPKLWEESGVSYRELIENLIELAIERNQRDQQLKSSRSDG
>Mature_366_residues
MKKKVGIVFGGKSAEHEVSLQSAKNIVDAIDSDKYDVYLLGIDKQGNWHVNDRSHYLINEEDPKLISLNKTNEGIAVIPG
AEKNQIVTSNSTKSLDQLDVIFPIVHGTLGEDGSLQGMLRMANIPFVGSSVLGSAISMDKDIAKRLLKDAGLKVAKGYAY
RSVDKESIHFDSLKEELGLPMFIKPANQGSSVGVHKVENEEQFYSAIKDAFQFDHKLLVEEAIVGREIECAVLGNEKPAA
SVPGEILPTGDFYSYEAKYIDETGAVLQIPAKLEEPVVDSIQDIALQAFKALNCEGLARVDVFLTDDNEIVINEINTLPG
FTKISMYPKLWEESGVSYRELIENLIELAIERNQRDQQLKSSRSDG

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=361, Percent_Identity=60.6648199445983, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1786280, Length=354, Percent_Identity=34.4632768361582, Blast_Score=183, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_OCEIH (Q8ERJ6)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692227.1
- ProteinModelPortal:   Q8ERJ6
- SMR:   Q8ERJ6
- GeneID:   1017484
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1306
- NMPDR:   fig|221109.1.peg.1310
- HOGENOM:   HBG427092
- OMA:   CKAINAY
- ProtClustDB:   PRK01966
- BioCyc:   OIHE221109:OB1306-MONOMER
- BRENDA:   6.3.2.4
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 40373; Mature: 40373

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKVGIVFGGKSAEHEVSLQSAKNIVDAIDSDKYDVYLLGIDKQGNWHVNDRSHYLINE
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEECCCCEEEECC
EDPKLISLNKTNEGIAVIPGAEKNQIVTSNSTKSLDQLDVIFPIVHGTLGEDGSLQGMLR
CCCCEEEEECCCCCEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHE
MANIPFVGSSVLGSAISMDKDIAKRLLKDAGLKVAKGYAYRSVDKESIHFDSLKEELGLP
ECCCCEECHHHHHHHHCCCHHHHHHHHHHCCCEEECCCEEECCCCCCCCHHHHHHHCCCC
MFIKPANQGSSVGVHKVENEEQFYSAIKDAFQFDHKLLVEEAIVGREIECAVLGNEKPAA
EEEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
SVPGEILPTGDFYSYEAKYIDETGAVLQIPAKLEEPVVDSIQDIALQAFKALNCEGLARV
CCCCCEEECCCCCCEEEEEECCCCCEEEECHHHCCHHHHHHHHHHHHHHHHCCCCCEEEE
DVFLTDDNEIVINEINTLPGFTKISMYPKLWEESGVSYRELIENLIELAIERNQRDQQLK
EEEEECCCEEEEEECCCCCCCEEEECCHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHH
SSRSDG
HCCCCC
>Mature Secondary Structure
MKKKVGIVFGGKSAEHEVSLQSAKNIVDAIDSDKYDVYLLGIDKQGNWHVNDRSHYLINE
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEECCCCEEEECC
EDPKLISLNKTNEGIAVIPGAEKNQIVTSNSTKSLDQLDVIFPIVHGTLGEDGSLQGMLR
CCCCEEEEECCCCCEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHE
MANIPFVGSSVLGSAISMDKDIAKRLLKDAGLKVAKGYAYRSVDKESIHFDSLKEELGLP
ECCCCEECHHHHHHHHCCCHHHHHHHHHHCCCEEECCCEEECCCCCCCCHHHHHHHCCCC
MFIKPANQGSSVGVHKVENEEQFYSAIKDAFQFDHKLLVEEAIVGREIECAVLGNEKPAA
EEEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
SVPGEILPTGDFYSYEAKYIDETGAVLQIPAKLEEPVVDSIQDIALQAFKALNCEGLARV
CCCCCEEECCCCCCEEEEEECCCCCEEEECHHHCCHHHHHHHHHHHHHHHHCCCCCEEEE
DVFLTDDNEIVINEINTLPGFTKISMYPKLWEESGVSYRELIENLIELAIERNQRDQQLK
EEEEECCCEEEEEECCCCCCCEEEECCHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHH
SSRSDG
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376