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The map label for this gene is lipA

Identifier: 23098739

GI number: 23098739

Start: 1326507

End: 1327451

Strand: Direct

Name: lipA

Synonym: OB1284

Alternate gene names: 23098739

Gene position: 1326507-1327451 (Clockwise)

Preceding gene: 23098738

Following gene: 23098742

Centisome position: 36.54

GC content: 38.52

Gene sequence:

>945_bases
ATGTCTAATCAACAAACGCATGTACGTAAGCCAGATTGGTTAAAAACAAGAATTAATACGAACAAATCGTATCGAAATTT
AAAAAAGTTGATGCGTGATAATCGGTTAAACACGGTTTGTGAAGAAGCGCGCTGTCCAAACTTACATGAATGCTGGAGCG
AACGAAAAACAGCTACTTTTATGATTTTAGGAGATACATGTACAAGAGGTTGCCGGTTCTGTGCAGTAAAAACAGGGTTA
CCAAATGAACTAGATTGGGGAGAACCAGAACGTGTGGCTGATTCTGTCACCGTAATGGGGTTAAAACATGTGGTAGTAAC
AGCAGTGGCGAGAGATGATTTAAATGATGGCGGTGCAGCAGTGTTTGCGGAAACTGTCCGAGCTATTCGTAGAAAGAATC
CTGGTTGTACTATTGAAATTTTGCCTTCGGATATGAAAGGAGATTATGAAAGTCTCCATACATTAATGGATAGTGGACCA
GATATTTTTAATCATAATATTGAAACCGTACGAAGACTTACAAAACGAGTGCGAGCAAGAGCTATGTACGATCGTTCTTT
AGAGTTACTACGACGGGTAAAAGAGATAGCTCCTAATACACCGACGAAATCAAGTATCATGGTTGGCCTTGGAGAAGAAA
AAGATGAGATTATTCAGGCGATGGATGATTTATTAGCGCATAATGTGGATATTGTTACACTTGGCCAATATTTACAGCCA
ACGAAAAAGCATCTAGAGGTAGTACGCTATTACCATCCAGATGAATTTGAAGAATTGAAAAATATCGCCCTAGAAAAAGG
CTTTTCTCATTGTGAATCTGGTCCATTAGTTCGTTCCTCATACCATGCGGATGAACAAGTTTCTAATGCAGCAGCTCAAC
GAAGAATTAAGTATATGAAAGGTGTAGAAAAGCAAGAAAACAGTCAATTGGATTTTAATTTCTAA

Upstream 100 bases:

>100_bases
GAATTAGAGCCAATAACGTTGACAGAAGAACAGTGGAAAGAAGTGTACCAGATTGCCGAATCAAATTATTCAGAGAATAA
TATAAAAGGAGCTGTTTCAC

Downstream 100 bases:

>100_bases
GTACTAAGAATTTCAGGGTTTAGGACCCAAGAATATACTCAATAGATAAATGGATTTAAGAAATCGTTTCAGAAAATACT
GCGCTTACAACACCAACACG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MSNQQTHVRKPDWLKTRINTNKSYRNLKKLMRDNRLNTVCEEARCPNLHECWSERKTATFMILGDTCTRGCRFCAVKTGL
PNELDWGEPERVADSVTVMGLKHVVVTAVARDDLNDGGAAVFAETVRAIRRKNPGCTIEILPSDMKGDYESLHTLMDSGP
DIFNHNIETVRRLTKRVRARAMYDRSLELLRRVKEIAPNTPTKSSIMVGLGEEKDEIIQAMDDLLAHNVDIVTLGQYLQP
TKKHLEVVRYYHPDEFEELKNIALEKGFSHCESGPLVRSSYHADEQVSNAAAQRRIKYMKGVEKQENSQLDFNF

Sequences:

>Translated_314_residues
MSNQQTHVRKPDWLKTRINTNKSYRNLKKLMRDNRLNTVCEEARCPNLHECWSERKTATFMILGDTCTRGCRFCAVKTGL
PNELDWGEPERVADSVTVMGLKHVVVTAVARDDLNDGGAAVFAETVRAIRRKNPGCTIEILPSDMKGDYESLHTLMDSGP
DIFNHNIETVRRLTKRVRARAMYDRSLELLRRVKEIAPNTPTKSSIMVGLGEEKDEIIQAMDDLLAHNVDIVTLGQYLQP
TKKHLEVVRYYHPDEFEELKNIALEKGFSHCESGPLVRSSYHADEQVSNAAAQRRIKYMKGVEKQENSQLDFNF
>Mature_313_residues
SNQQTHVRKPDWLKTRINTNKSYRNLKKLMRDNRLNTVCEEARCPNLHECWSERKTATFMILGDTCTRGCRFCAVKTGLP
NELDWGEPERVADSVTVMGLKHVVVTAVARDDLNDGGAAVFAETVRAIRRKNPGCTIEILPSDMKGDYESLHTLMDSGPD
IFNHNIETVRRLTKRVRARAMYDRSLELLRRVKEIAPNTPTKSSIMVGLGEEKDEIIQAMDDLLAHNVDIVTLGQYLQPT
KKHLEVVRYYHPDEFEELKNIALEKGFSHCESGPLVRSSYHADEQVSNAAAQRRIKYMKGVEKQENSQLDFNF

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=297, Percent_Identity=45.7912457912458, Blast_Score=269, Evalue=3e-72,
Organism=Homo sapiens, GI37577164, Length=246, Percent_Identity=46.7479674796748, Blast_Score=232, Evalue=3e-61,
Organism=Escherichia coli, GI1786846, Length=287, Percent_Identity=50.1742160278746, Blast_Score=286, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI32564533, Length=288, Percent_Identity=48.6111111111111, Blast_Score=255, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6324770, Length=302, Percent_Identity=47.682119205298, Blast_Score=278, Evalue=9e-76,
Organism=Drosophila melanogaster, GI221513272, Length=306, Percent_Identity=47.3856209150327, Blast_Score=276, Evalue=2e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_OCEIH (Q8ERL8)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692205.1
- ProteinModelPortal:   Q8ERL8
- GeneID:   1017462
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1284
- NMPDR:   fig|221109.1.peg.1288
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- BioCyc:   OIHE221109:OB1284-MONOMER
- BRENDA:   2.8.1.8
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 35892; Mature: 35761

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQQTHVRKPDWLKTRINTNKSYRNLKKLMRDNRLNTVCEEARCPNLHECWSERKTATF
CCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHCCCCEEE
MILGDTCTRGCRFCAVKTGLPNELDWGEPERVADSVTVMGLKHVVVTAVARDDLNDGGAA
EEECCHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VFAETVRAIRRKNPGCTIEILPSDMKGDYESLHTLMDSGPDIFNHNIETVRRLTKRVRAR
HHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHH
AMYDRSLELLRRVKEIAPNTPTKSSIMVGLGEEKDEIIQAMDDLLAHNVDIVTLGQYLQP
HHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEECCHHHCH
TKKHLEVVRYYHPDEFEELKNIALEKGFSHCESGPLVRSSYHADEQVSNAAAQRRIKYMK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
GVEKQENSQLDFNF
CCCHHCCCCCCCCC
>Mature Secondary Structure 
SNQQTHVRKPDWLKTRINTNKSYRNLKKLMRDNRLNTVCEEARCPNLHECWSERKTATF
CCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHCCCCEEE
MILGDTCTRGCRFCAVKTGLPNELDWGEPERVADSVTVMGLKHVVVTAVARDDLNDGGAA
EEECCHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VFAETVRAIRRKNPGCTIEILPSDMKGDYESLHTLMDSGPDIFNHNIETVRRLTKRVRAR
HHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHH
AMYDRSLELLRRVKEIAPNTPTKSSIMVGLGEEKDEIIQAMDDLLAHNVDIVTLGQYLQP
HHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEECCHHHCH
TKKHLEVVRYYHPDEFEELKNIALEKGFSHCESGPLVRSSYHADEQVSNAAAQRRIKYMK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
GVEKQENSQLDFNF
CCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376